EBI Dbfetch

ID   D11455; SV 1; linear; genomic DNA; STD; PRO; 1595 BP.
XX
AC   D11455;
XX
DT   09-JUL-1992 (Rel. 32, Created)
DT   31-MAY-2008 (Rel. 96, Last updated, Version 9)
XX
DE   Pseudomonas fluorescens gene for triacylglycerol lipase, complete cds.
XX
KW   .
XX
OS   Pseudomonas fluorescens
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
XX
RN   [1]
RP   1-1595
RA   Rhee J.S.;
RT   ;
RL   Submitted (17-JUN-1992) to the EMBL/GenBank/DDBJ databases.
RL   Contact:Joon Shick Rhee Korea Advanced Institute of Science and Technology,
RL   Dept. of Biotechnology; Yusung-Gu, Kusung-Dong 373-1, Taejon 305-701, Korea
XX
RN   [2]
RX   PUBMED; 1368740.
RA   Chung G.H., Lee Y.P., Jeohn G.H., Yoo O.J., Rhee J.S.;
RT   "Cloning and nucleotide sequence of thermostable lipase gene from
RT   Pseudomonas fluorescens SIK W1";
RL   Agric. Biol. Chem. 55(9):2359-2365(1991).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1595
FT                   /organism="Pseudomonas fluorescens"
FT                   /strain="SIK W1"
FT                   /mol_type="genomic DNA"
FT                   /clone_lib="pUC19"
FT                   /clone="pJH92"
FT                   /db_xref="taxon:294"
FT   RBS             143..146
FT   CDS             154..1503
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /product="triacylglycerol lipase"
FT                   /EC_number="3.1.1.3"
FT                   /note="triacylglycerol acylhydrolase"
FT                   /db_xref="GOA:P26504"
FT                   /db_xref="InterPro:IPR001343"
FT                   /db_xref="InterPro:IPR002921"
FT                   /db_xref="InterPro:IPR011049"
FT                   /db_xref="InterPro:IPR018511"
FT                   /db_xref="InterPro:IPR018512"
FT                   /db_xref="UniProtKB/Swiss-Prot:P26504"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT                   /protein_id="BAA02012.1"
FT                   /translation="MGVFDYKNLGTEASKTLFADATAITLYTYHNLDNGFAVGYQQHGL
FT                   GLGCRHTGRGVARQHRLPGSDPPAFPGILTRKRPPWTRCTQPVGRQSSASALGYGGKVD
FT                   ARGTFFGEKAGYTTAQAEVLGKYDDAGKLLEIGIGFRGTSGPRESLITTPCRSGQRPAR
FT                   RAGPQGLCEKLCRRTFGGLLKTVADYAGAHGLSGKDVLVSGHSLGGLAVNSMADLSTSK
FT                   WAGFYKDANYLAYASPTQSAGDKVLNIGYENDPVFRALDGSTFNLSSLGVHDKAHESTT
FT                   DNIVSFNDHYASTLWNVLPFSIANLSTWVSHLPSAYGDGMTRVLESGFYEQMTRDSTII
FT                   LCPTWSDPARANTWVQDLNRNAEPHTGNTFIIGSDGNDLIQGGKGADFIEGGKGNDTIR
FT                   DNSGHNTFLFSGHFGQDRIIGYQPTGWCSRAPTAAPTCATTRRPWGPIRC"
FT   sig_peptide     154..222
XX
SQ   Sequence 1595 BP; 331 A; 508 C; 486 G; 270 T; 0 other;
     aagcttgcat gcctgcagac aagccccctg ttgcaaacaa accgcctgag gcaagccgac        60
     cccctgtggc gagcgggctt gcccgttggg ctgcgcagca gcaataaaac cacacaatct       120
     ccggccaatt ccaacaacaa gagagacaag acaatgggtg tatttgacta caagaacctc       180
     ggcaccgaag ccagcaaaac cttgttcgcc gatgccaccg caatcacgtt gtatacctat       240
     cacaacctgg ataacggctt cgcagtcggc taccagcaac atggcttggg gctcggctgc       300
     cggcacactg gtcggggcgt tgctcggcag cacagactcc cagggagtga tcccccggca       360
     ttccctggaa tcctgactcg gaaaaggccg ccctggacgc ggtgcacgca gccggttgga       420
     cgccaatcaa gcgccagcgc actgggctac ggcggcaagg tggatgcgcg gggcactttt       480
     tttggcgaga aggccggcta caccacggcc caggccgaag tgctgggcaa gtacgatgac       540
     gccggcaaac tgctcgagat cggcatcggt tttcgtggca cctcgggccc tcgggaaagc       600
     ctgattacga ctccatgccg atctggtcag cgacctgctc gccgcgctgg gccccaagga       660
     ctatgcgaaa aactatgccg gcgaacgttt ggcggcttgc tcaagacggt ggccgactat       720
     gccggcgccc atggcctgag tggcaaggat gtgctggtca gcggccacag cctgggcggc       780
     ctggcggtca acagcatggc cgacctgagc accagcaaat gggcgggttt ctacaaggac       840
     gccaactacc tggcctacgc ctcgcccacc cagagcgccg gcgataaggt cctgaatatc       900
     ggctacgaaa acgacccggt attccgtgcg ctggacggct ccaccttcaa cctgtcgtcc       960
     ctcggcgtgc atgacaaggc ccacgagtcg accaccgaca acatcgtcag cttcaacgac      1020
     cactacgcct cgacgttgtg gaatgtgctg ccgttttcca tcgccaacct gtcgacctgg      1080
     gtgtcgcatt tgcccagcgc ttacggcgac ggcatgacgc gtgtgctgga atcggggttc      1140
     tacgagcaaa tgacccgtga ctcgacgatt atcctgtgcc caacctggtc cgacccggcg      1200
     cgcgccaaca cctgggtcca ggacctcaac cgcaatgccg agccgcacac aggcaatacc      1260
     ttcatcatcg gcagcgacgg caatgacctg atccagggcg gcaagggcgc ggacttcatc      1320
     gaaggcggca agggcaatga cacgatccgc gacaacagcg ggcacaacac ctttttgttc      1380
     agcgggcatt ttggccagga tcggattatc ggctaccagc cgacaggctg gtgttccagg      1440
     gcgccgacgg cagcaccgac ctgcgcgacc acgcgaaggc cgtgggggcc gatacggtgc      1500
     tgagttttgg cgccgactcg gtgacctggt cggggtcggg ctgggcggcc tgtggagcga      1560
     gggtgtgctg atccccgggt accgagctcg aattc                                 1595
//