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EBI DbfetchID D11101; SV 1; linear; genomic DNA; STD; PRO; 5183 BP. XX AC D11101; XX DT 15-MAY-1992 (Rel. 31, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 18) XX DE Streptococcus sanguinis genes for StsI restriction endonuclease and StsI DE methylase, complete cds. XX KW . XX OS Streptococcus sanguinis OC Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Streptococcus. XX RN [1] RP 1-5183 RA Kita K.; RT ; RL Submitted (11-MAY-1992) to the EMBL/GenBank/DDBJ databases. RL Keiko Kita, Tottori University, Department of Biotechnology; 4-101 Koyama, RL Tottori, Tottori 680, Japan (Tel:81-857-28-0321(ex.4442), RL Fax:81-857-31-0881) XX RN [2] RP 1-5183 RX DOI; 10.1093/nar/20.16.4167 RX PUBMED; 1387204. RA Kita K., Suisha M., Kotani H., Yanase H., Kato N.; RT "Cloning and sequence analysis of the StsI restriction-modification gene: RT presence of homology to FokI restriction-modification enzymes"; RL Nucleic Acids Res. 20(16):4167-4172(1992). XX FH Key Location/Qualifiers FH FT source 1..5183 FT /organism="Streptococcus sanguinis" FT /isolate="#54" FT /mol_type="genomic DNA" FT /clone="pSts627" FT /note="synonym: Streptococcus sanguis" FT /db_xref="taxon:1305" FT CDS 555..2363 FT /codon_start=1 FT /transl_table=11 FT /product="StsI restriction endonuclease" FT /db_xref="GOA:P29346" FT /db_xref="HSSP:2FOK" FT /db_xref="InterPro:IPR004234" FT /db_xref="UniProtKB/Swiss-Prot:P29346" FT /protein_id="BAA01875.1" FT /translation="MTISINEYSDLNNLAFGLGQDVSQDLKELVKVASIFMPDSKIHKW FT LIDTRLEEVVTDLNLRYELKSVITNTPISVTWKQLTGTRTKREANSLVQAVFPGQCSRL FT AIVDWAAKNYVSVAVAFGLLKFHRADKTFTISEIGIQAVKLYDSEELAELDKFLYERLL FT EYPYAAWLIRLLGNQPSKQFSKFDLGEHFGFIDELGFETAPIEIFLNGLAQAEIDGDKT FT AAQKIKSNFESTSDKYMRWLAGVLVTAGLATSTTKKVTHTYKNRKFELTLGTVYQITAK FT GLTALKEVNGKSRYPRSRKRVMWEFLATKDKEAIAKKTSRSLMLKHLTEKKNPIQAEVI FT ATLINTDYPTLEITPEEVIDDCIGLNRIGIEILIDGDKLTLNDKLFDFEIPVQKDVVLE FT KSDIEKFKNQLRTELTNIDHSYLKGIDIASKKKTSNVENTEFEAISTKIFTDELGFSGK FT HLGGSNKPDGLLWDDDCAIILDSKAYSEGFPLTASHTDAMGRYLRQFTERKEEIKPTWW FT DIAPEHLDNTYFAYVSGSFSGNYKEQLQKFRQDTNHLGGALEFVKLLLLANNYKTQKMS FT KKEVKKSILDYNISYEEYAPLLAEIE" FT CDS complement(2489..4450) FT /codon_start=1 FT /transl_table=11 FT /product="StsI methylase" FT /db_xref="GOA:P29347" FT /db_xref="InterPro:IPR002052" FT /db_xref="UniProtKB/Swiss-Prot:P29347" FT /protein_id="BAA01876.1" FT /translation="MRYIGSKKLLLPEIKKMVDKHTDGSEEVFLDLFAGTNVVANYFKQ FT FYTVYSNDMLFFSYVNAKATIENNSKPSFSKLIQAGISSPMTYLQNLEVNDETIGYYEV FT AYSPTGEANYLSVHNAKKLDIIRSQIESWKNQNLLTEHEYYYLLSSLIEALPFISNTTG FT TYGAFLKHWDKRSLNDLELQDFTIFDNSKQNKAFNEDANELVQKIKADIVYIDTPYNSR FT QYASNYHLLENVARNEHPTLKGITKIFDWKNLKSDYATKGKALVAMRDLIQNINSTHII FT LSYNNEGIISEEDLTNILKEFSVDGIVDIKKIPYRKYQSKNVSKNKEIYELLFYIQRKP FT FSKNKTLNKPLNNVRVSSTKKYIKSPLNYIGGKYKLLNQILPLFPKNINTFVDIFSGGA FT NVGINVKAKKYIFNDMNTRINEMFRYFQTQPPVKLVQQIEEKIDEWGLSKTNEDAFLAF FT RKHYNTNPNPLDLYVLSSFSYNYQFRFNNSMEFNNPFGRNRSHFSENMRNNLLNFVTKL FT QTLDATFTDNYFNEFDFSNLTSNDFIYLDPPYLITTGSYNDGKRGFSDWNNTSEMKLLN FT FMDYLNQHGIRFALSNVTEHKGKTNQLLKDWAYSRNLNVNYLDHNYNNSSHNSKSKGSQ FT EVLITNYETKTFNLLNTK" XX SQ Sequence 5183 BP; 1779 A; 745 C; 874 G; 1785 T; 0 other; agtatatctg caaaatttaa aagattcatc cgcttacttt ttaatattat ccagttagag 60 agggataaaa gctgaatcgg cgaacacctc tgtatttcga caataaaaca gccactgata 120 gaagattttg ctaaaaatgg ctgtttttcc ttattttatc gttcagaatg agagtttaaa 180 aaagaattga attattaact aaaaagataa ttatggtata ataggtacat ttaggaggtt 240 cactatggtt gaatttactc ttgaaccttt ttctaacaac acttttaaat tacttaaatc 300 attgaaagaa aaccaagttg aagtaaaagt catttattat ataccgctct ctcaacaaga 360 gattgcaaac attaacaaaa tgtaaaactg aaaacgaata gacttctgcg tgatttgatt 420 gaaggcggct ttgtctgccc ttatcaaaat aaacgtggaa aatatgaaat cactgaaaaa 480 gtgcataagg tattgcgtct gattcagaag aaaaatatat agaaaaataa agaaataaaa 540 atttaggagg aaatatgaca ataagcatta atgaatacag tgatttgaat aacttagcgt 600 ttggtttagg gcaagatgtt agtcaggatt taaaagagtt agtaaaagtt gcttcaattt 660 ttatgcctga ttcaaaaatt cataaatggt tgatagatac gagattagaa gaagtagtta 720 cagatttaaa tcttcgatat gaattaaaat ctgtaattac taatacgcct atttcagtta 780 cttggaaaca acttactgga acaagaacga agagagaagc taatagttta gttcaagcag 840 tatttccagg acaatgttca aggcttgcaa ttgtagattg ggctgctaaa aattatgtga 900 gtgtagcagt tgcttttgga ctattgaaat tccatcgggc agataaaact ttcacgattt 960 cagagatagg aattcaagca gtaaagttat atgattctga agaattggca gaactggaca 1020 aatttttata tgaaaggcta cttgagtatc cgtatgcagc gtggttgata agattattag 1080 ggaatcaacc ctctaaacag ttttcaaaat ttgatttagg agaacatttt gggtttatag 1140 atgaacttgg atttgaaaca gcccctatcg aaatattttt aaatggacta gcccaggctg 1200 agattgatgg cgataaaaca gcagctcaaa aaatcaaatc aaacttcgaa agtacctctg 1260 ataagtatat gagatggcta gctggtgttt tggttactgc aggattggca acttccacta 1320 ctaaaaaagt tacccataca tacaaaaacc gtaaatttga acttactctt ggtacagtgt 1380 atcagatcac agctaaagga ttaactgcct taaaggaggt taacggaaaa tcaagatatc 1440 ctcgaagtag gaaaagagtg atgtgggaat tcttagcaac aaaggataaa gaagcaatcg 1500 ctaaaaaaac atcacgatct ctaatgttaa aacaccttac tgaaaagaaa aatccaattc 1560 aagcagaggt gattgctact ttaattaaca cagattatcc aactcttgaa ataacacctg 1620 aagaagtgat agatgactgt attgggctta atagaattgg tattgaaatt ctgattgatg 1680 gagataagct aacgttaaat gataaattgt ttgattttga aattcctgtg cagaaagatg 1740 tggtattaga aaaaagtgat attgaaaaat ttaagaatca attgcgtacg gaactaacca 1800 atattgacca ttcttatctt aaaggaattg atatagctag taaaaagaaa accagtaatg 1860 ttgaaaatac ggaatttgaa gcaatatcaa ccaagatttt tacggatgag ttgggttttt 1920 caggcaaaca tctaggagga agcaacaaac cagatggact cctgtgggat gatgattgtg 1980 caattattct tgattcaaaa gcttactcag aaggctttcc actcactgcc tcccacacag 2040 atgctatggg aagatatttg aggcaattta cagagcgaaa agaagaaata aagccaacgt 2100 ggtgggatat tgctccagaa catttagaca atacatattt cgcttacgtt tctgggagtt 2160 tttcgggtaa ttataaggaa cagttacaaa aatttaggca agatacaaac catttaggtg 2220 gggcactaga gtttgttaaa ttgttattac tagcaaataa ttataaaact caaaaaatga 2280 gtaaaaaaga agttaagaaa agtattcttg attataatat ttcatatgaa gaatatgctc 2340 cattacttgc agaaatagag taagagaagt tatcgggata tggattgttt aaataataaa 2400 agatgatttt cgttttgaaa agcatctttt ttcatcttgc ttgaagttat tatgtattga 2460 ggttggtatg ggatagtatg tagttcttct attttgtatt aagcagatta aatgttttag 2520 tttcataatt agttattaga acttcttgac tacctttgct ttttgaatta tgagacgaat 2580 tgttataatt atgatcaaga taattgacat ttagatttct tgaataagcc caatctttta 2640 acaattgatt tgttttacct ttgtgttcag ttacattact gagagcaaat cgaataccat 2700 gttgatttag ataatccata aaatttagta acttcatttc agaagtatta ttccaatctg 2760 aaaatcctcg ttttccatca ttataacttc cagttgtaat taaatatgga ggatcaagat 2820 aaataaaatc attagatgtc aaattcgaaa aatcaaattc attaaaatag ttatctgtga 2880 acgtggcatc taatgtttga agcttagtaa caaaatttaa taaattattt ctcatgtttt 2940 cactaaagtg actacgattt ctaccaaaag gattattgaa ttccatagag ttattgaatc 3000 taaattggta attgtaacta aatgaggata gaacatataa atctaaagga ttaggatttg 3060 tattataatg tttcctgaaa gctaaaaatg catcttcatt agttttactc aatccccact 3120 catcaatctt ttcttcgatt tgttgaacta atttgacagg tggttgagtt tgaaaatatc 3180 gaaacatttc gttgatacga gtattcatat cattaaaaat atatttctta gctttaacat 3240 ttatgcctac attagcgcct ccactaaata tatctacgaa cgtattaatg ttcttaggaa 3300 aaaggggaag tatttgattt aatagtttat attttcctcc aatgtaatta agtggacttt 3360 taatatattt ttttgttgaa ctgactctga cgttatttag aggtttgttc agagttttat 3420 ttttagaaaa tggctttctt tggatataaa ataataattc ataaatttct ttgttcttag 3480 aaacattctt tgattgatac tttcgatatg gaattttttt aatatcaact atgccatcaa 3540 cagaaaattc tttcaaaata ttagtcaaat cctcttcgga gataatacct tcattattgt 3600 aactcaaaat tatatgagtt gaatttatat tttgaattaa atctctcatt gctaccagag 3660 ccttgccttt tgtagcgtaa tcacttttaa ggtttttcca atcgaaaatt tttgtaatac 3720 cttttaaagt tgggtgttca tttctagcga cattttccag taaatgataa ttagaggcat 3780 attgtcttga attataaggg gtatcaatat agacaatatc tgcctttatt ttttgaacta 3840 gttcattcgc atcttcattg aatgctttgt tttgttttga gttatcaaat atagtaaaat 3900 cttgtaattc taaatcattt aaagatcttt tatcccagtg ttttaggaaa gcgccataag 3960 ttcctgtagt gttgctaata aatggcaatg cttctatgag agaagagagt agataataat 4020 attcgtgttc tgttaataaa ttttgattct tccaactctc aatttgacta cgaattatat 4080 ccagtttttt tgcattatgt actgaaaggt aatttgcctc tccagtgggc gaataagcaa 4140 cttcgtagta tcctatcgtt tcatcgttga cttctaaatt ttgtaaataa gtcataggag 4200 aactgatacc tgcctgaata agtttagaaa atgatggttt tgaattattt tcaattgtag 4260 ctttagcatt gacatagcta aaaaataaca tgtcgttcga atagactgtg taaaattgtt 4320 taaaataatt tgcaactaca tttgtaccag caaataaatc taagaacact tcctcagagc 4380 catcagtatg tttatctacc atttttttaa tttcaggtag aagtaatttt tttgaaccaa 4440 tatatctcat ttttacatcc tttatcatcc ttattttatc ataatagaag gaaaaatcca 4500 actatgaata tatatttttc tgccttgata gtttaaatta cgcatgttgg cccactccaa 4560 cacctgtaca ttctcctcat cgatcaatga aactctttcc tctaccaatt cctgatagag 4620 aatatcttga atattcttct gatttaaaat tattgagact agcgaccacc tcgctagttt 4680 tctatatcgg aggatatgac attctttggc aatggcatat agtctcgttt taggaagttc 4740 aaggacctat tcacgtttgt taaaggaaag aacaccatct tcctcgttaa atttgaaata 4800 agagctgtca acagcatctc ttgccttctc ggtttcttcc ttcaaaactt ccatgtaatg 4860 ggaacttggt tttggtaata ctaattcttt ttgccttact tcagatgaag taaggttttt 4920 ctttgtcttt aaaatctaaa taaagataga ttctgcatct ttttgagaag tgggataatc 4980 ccatgtctgt tctccagtta cgaatatagg tgaaaaaacc aatatagtct tgtagatact 5040 tagtggatac atttctcata tcacggtaga tatacttctt aatctcttta tggagtccat 5100 ttactcaacc aagggataag ccattctggt atttagttcg gtgaaggtat caaatagcat 5160 ttctccctat tggtaatatg atg 5183 // |
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