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EBI DbfetchID D10671; SV 1; linear; genomic DNA; STD; PRO; 3353 BP. XX AC D10671; XX DT 16-OCT-1992 (Rel. 33, Created) DT 14-DEC-1998 (Rel. 58, Last updated, Version 6) XX DE Acinetobacter calcoaceticus AccI restriction-modification genes, complete DE cds. XX KW AccI methylase; AccI restriction endonuclease; deoxyribonuclease; KW methyltransferase; type II restriction-modification system. XX OS Acinetobacter calcoaceticus OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Moraxellaceae; Acinetobacter. XX RN [1] RP 1-3353 RX PUBMED; 1368703. RA Kawakami B., Christophe H., Nagatomo M., Oka M.; RT "Cloning and nucleotide sequences of the AccI restriction-modification RT genes in Acinetobacter calcoaceticus"; RL Agric. Biol. Chem. 55(6):1553-1559(1991). XX FH Key Location/Qualifiers FH FT source 1..3353 FT /organism="Acinetobacter calcoaceticus" FT /mol_type="genomic DNA" FT /clone_lib="pUC18" FT /clone="pAccIRM8" FT /db_xref="taxon:471" FT CDS 569..1669 FT /codon_start=1 FT /transl_table=11 FT /gene="R-AccI" FT /product="AccI restriction endonuclease" FT /db_xref="GOA:P24546" FT /db_xref="InterPro:IPR019054" FT /db_xref="UniProtKB/Swiss-Prot:P24546" FT /protein_id="BAA01522.1" FT /translation="MDYYDRIRELTKNVPVELVDFEQPRDLARTPTQASSNFITNKEQG FT DWAEDLVTRAINENSKNFVAVKYGKSDNLVAGENGFDTFYQDFQTELDTIGKRPDLLIF FT KKTDFDTTLGFDVSQIPHHQITDYVKKAIAGIEVRSSAFLIDKYEEAMQVRTQRFTEIA FT FQTRDKILAEFLDVLDHPSRSKYITLLNTLTLETISIFDFKVPGWRSNERLIEVNNLFK FT RLKVAIKEIQKRDYLSITPKVEDIKVVYKWIETFNVPHFYFQVFFDKVYGISFEQILTI FT ISNSDNDGVIFSVEKDVQNQNKTTIKINSKTGYPIASKVDEPTHESIRKEMDRGRLLFY FT VTFKGGTAYLDLDNLRTILGIEEAEF" FT CDS 1669..3291 FT /codon_start=1 FT /transl_table=11 FT /gene="M-AccI" FT /product="AccI methylase" FT /note="consensus tetra-amino acids of N6-adenine FT methylases, NPPY, at nt 2035-2046" FT /db_xref="GOA:P25201" FT /db_xref="InterPro:IPR002052" FT /db_xref="UniProtKB/Swiss-Prot:P25201" FT /protein_id="BAA01523.1" FT /translation="MIKTTEHIVNNSIERDYSKSISLEHRKKFAQFFTPFPIAYAMAKW FT ILGNKQLKTVLEPAFGLGVFSRAILSQQKEINIKAFEVDETIFENAKEYFDDFENVNIL FT LQDYMYNDWKNKYDGIICNPPYFKFHDYDNKNILKEIETNLKCKLNGFTNLYTLFLLKS FT IHQLSQNGRCAYIIPSEFLNSDYGKLVKTYLIKSKTLRHIIVIDFEENVFDDALTTASI FT ILCANDNITDKVQFNNIQSLQDLSKIDEIINKYPNFLETEQTYNFSDLNPEIKWKAYYQ FT KQNSIKFKNLVPFSTYAKVVRGIATGSNEYFTFNLSKAKEFNIDEQYLLPCICSAKDAK FT TSFFTKQDFEELKKSDKSVFLFNAQNSTDKNISSYIQKGESEEINKRFLTASRTPWYSL FT ENRKPAPIWVSVFNRSGLRFIRNEANISNLTSYHCIIQNKQVVSEIDIDLLFAYLLTDT FT AKQIFEDNSRQYGNGLQKFEPNDLNKGMMLDLGLLDKQTSDEILNLYKEYKYLILDNKN FT GDEIINKIDKILTDKYSEKKHWA" XX SQ Sequence 3353 BP; 1262 A; 507 C; 516 G; 1068 T; 0 other; ggatccaatt ttgtaggaat tttcccgaaa tgtagcgaca ccgcagcgac gtgtctggag 60 taattttgaa aggtactttg gcttcttccc aacaccgaaa ccgtaccttc aaacctttgc 120 aaaagctccg caaatcccgg aacttcgcgc tttgcctgat tgatgatttt gttttctcta 180 agctcttcta ccctcttttt tttgttgcca ttttactgat ttttaattag ttttacaaag 240 taactaaata tggcgctgag aaagctaccg aagttttagt tcaacatagg ttttgcaaaa 300 tgcggggata agtctaagtt gaactatttt agatatttat ccgcaaattg gcttttcatc 360 ttgatttttt ttattaattt aacaatctga aaaagccatt tgctacgttt ctgaattctt 420 gaacttttgg ttcttcgaat tcccgcactt cgcaaagcct ttttccgttt atgcccaatg 480 ctatgagcaa tcgcacaaat actaacccac aataaaaacc gacaaaaaaa gtttaacttt 540 gtagaataaa gtttcataat aaaagaagat ggattattac gacagaatta gagaattaac 600 aaaaaatgtt cctgtagaat tggttgattt tgaacaacca agagacttgg caagaacacc 660 aacacaagca tcttcaaatt ttattacaaa taaagagcaa ggtgattggg ctgaagacct 720 tgttacaagg gcaattaatg aaaattctaa aaattttgta gctgtaaaat atgggaaatc 780 tgacaattta gttgcaggag aaaatggctt tgatactttt tatcaagatt ttcaaactga 840 acttgacacc ataggaaaac gccccgactt gttaatcttt aaaaagacag attttgatac 900 tactttaggt tttgatgtta gtcaaattcc acatcatcaa atcactgatt atgtaaaaaa 960 agcaattgca ggaattgaag ttcgctcaag tgcttttttg attgacaagt acgaagaagc 1020 aatgcaagta agaactcaaa ggtttactga aattgcattt caaacaagag ataaaatatt 1080 agccgaattt ttagatgttt tagaccatcc aagtagaagt aagtacatca ctttattaaa 1140 tactttaacc cttgaaacta tttcaatttt tgactttaaa gttccaggtt ggaggtctaa 1200 tgaaagactt atagaagtaa ataatttatt caaaagacta aaggttgcaa taaaagaaat 1260 tcaaaagaga gattatcttt caataacacc aaaagttgaa gacataaaag tggtttacaa 1320 atggattgaa acattcaatg ttccacattt ttactttcaa gtgtttttcg acaaagtgta 1380 tggaatttca tttgaacaaa ttctaactat aatttcaaat tctgataatg atggtgtaat 1440 tttttccgtt gaaaaagatg tacaaaatca aaataaaaca acgataaaaa tcaactcaaa 1500 aactggttat ccaattgctt caaaggttga tgaaccaact cacgaaagca tcaggaaaga 1560 aatggacaga ggtcgattat tattttatgt aactttcaaa ggtggaactg catatcttga 1620 tttagataat ttacgaacta ttttaggaat agaagaagct gaattttaat gataaaaaca 1680 acggaacata ttgtcaataa ttccattgaa agagattact caaaatcaat ttcattagaa 1740 catcgtaaaa aattcgcaca attttttacg cctttcccca ttgcttatgc aatggctaaa 1800 tggattttag gcaacaaaca attaaaaacc gttcttgaac ctgctttcgg tcttggcgtt 1860 ttttcaagag cgattttaag ccaacagaaa gaaatcaata taaaagcttt tgaagtagat 1920 gaaacgatat ttgaaaacgc aaaagaatat tttgatgatt ttgaaaatgt aaatatactt 1980 ttgcaagatt atatgtacaa tgattggaaa aacaaatatg atggaattat ttgcaatccg 2040 ccctacttta agtttcacga ttatgacaat aaaaatattt taaaagaaat tgaaactaat 2100 cttaaatgca agctgaatgg ctttacaaat ctttatactt tatttttact aaaatcaatt 2160 catcagttaa gtcaaaatgg acgttgtgca tatataattc cttccgaatt tttgaattct 2220 gattacggaa aattagtgaa aacttattta ataaaaagta aaacattacg gcacattatt 2280 gtaattgact ttgaagaaaa tgtatttgat gatgctttaa caaccgcatc aattattctt 2340 tgtgcaaatg ataatataac agataaagtt cagtttaaca atatccaatc attgcaagat 2400 ttaagcaaaa tagatgaaat aataaataaa tatcctaact ttttagaaac cgaacagacc 2460 tataattttt ctgatttaaa tccagaaatt aaatggaagg cttactatca aaaacaaaat 2520 agcattaagt ttaaaaatct tgtgccattt tcgacttacg caaaagtagt tcgtggtata 2580 gcaacaggtt caaatgaata ttttactttt aatctatcaa aagccaaaga atttaatatt 2640 gacgaacaat atttattgcc ttgtatttgt agtgcaaaag atgctaaaac atcatttttc 2700 actaaacaag attttgaaga attaaagaaa agtgataaat ctgtttttct tttcaatgct 2760 caaaattcaa cggataaaaa tataagttca tatatccaaa aaggtgaaag cgaagagata 2820 aataaacggt ttcttactgc aagtagaaca ccttggtatt cattagaaaa tagaaaacct 2880 gcacccattt gggtttctgt ttttaatagg tcaggtttgc ggtttattag aaatgaagca 2940 aatatttcta acctaacttc ttatcattgc attatccaaa acaaacaagt tgtttcagaa 3000 attgacattg atttactttt tgcttatcta ttaacagaca cagctaaaca aatttttgaa 3060 gacaacagtc gtcaatacgg taatggactt caaaaatttg aaccaaatga ccttaataaa 3120 ggaatgatgc ttgatttagg attacttgac aagcagacat cagatgaaat tttaaatctt 3180 tacaaggaat acaaatactt aatccttgac aataaaaacg gagacgaaat aattaataaa 3240 atagataaaa tattaaccga caaatacagc gaaaaaaagc actgggcata ataactaagt 3300 tttcgtcttg tccgaaggac agcgagatga aaaaccctgt tgaacggaag ctt 3353 // |
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