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EBI DbfetchID D10668; SV 1; linear; genomic DNA; STD; PRO; 2492 BP. XX AC D10668; D01166; XX DT 09-MAR-1992 (Rel. 31, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Haemophilus parainfluenzae Hpal restriction-modification genes, complete DE cds. XX KW HpaI endonuclease; HpaI methyltransferase; KW HpaI restriction-modification system. XX OS Haemophilus parainfluenzae OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; OC Pasteurellaceae; Haemophilus. XX RN [1] RP 1-2492 RA Ito H.; RT ; RL Submitted (13-SEP-1991) to the EMBL/GenBank/DDBJ databases. RL Hiroyuki Ito, Takara Shuzo Co.,Ltd., Bioproducts Development Center; Seta RL 3-4-1, Otsu, Shiga 520-21, Japan (Tel:0775-43-7200, Fax:0775-43-2494) XX RN [2] RP 1-2492 RA Ito H.; RT ; RL Unpublished. XX RN [3] RX DOI; 10.1093/nar/20.4.705 RX PUBMED; 1542567. RA Ito H., Shimato H., Sadaoka A., Kotani H., Kimizuka F., Kato I.; RT "Cloning and expression of the HpaI restriction-modification genes"; RL Nucleic Acids Res. 20(4):705-709(1992). XX FH Key Location/Qualifiers FH FT source 1..2492 FT /organism="Haemophilus parainfluenzae" FT /mol_type="genomic DNA" FT /clone="pHPA2" FT /db_xref="taxon:729" FT RBS 358..361 FT /note="put. rRNA binding site" FT CDS 368..1132 FT /codon_start=1 FT /transl_table=11 FT /product="HpaI endonuclease" FT /EC_number="3.1.21.4" FT /db_xref="GOA:P29537" FT /db_xref="UniProtKB/Swiss-Prot:P29537" FT /protein_id="BAA01518.1" FT /translation="MKYEEINFKVPVESPYYPNYSQCVIERIYSILRNQKDMGDDRIII FT NTNLKKGLPLENINKIAGPMIEAWAEEVFSGIRDNRDNQYNLINVEAQERLGISDIILQ FT FQVNNNVITGNVDVKATSNDIPDSGKSPNITSFSRIRTAYVKDPNFIFIILSIKHSVYV FT KRNEYTNLMDGIMQIIDFNVYDLKYISDSDISYNPALGTGQIQIKDIHYVSSQKRTTWQ FT MCQLLDLKYLRSKKRTIEQFYNEAKRNKWIKD" FT RBS 1100..1103 FT /note="put. rRNA binding site" FT CDS 1117..2061 FT /codon_start=1 FT /transl_table=11 FT /product="HpaI methyltransferase" FT /EC_number="2.1.1.72" FT /db_xref="GOA:P29538" FT /db_xref="HSSP:1G60" FT /db_xref="InterPro:IPR002052" FT /db_xref="UniProtKB/Swiss-Prot:P29538" FT /protein_id="BAA01519.1" FT /translation="MDQRLICSNAIKALKNLEENSIDLIITDPPYNLGKDYGTTDDNLN FT FNKYLEFSHEWLEECYRVLKPHGTIYIFMGMKYISYIYKILEQDLGMYFNSWITWYYTQ FT GIGKTRGYSPRHDDILMFTKHPKKFTFNLDRIRVPQKYYRSVNNMRGANPSNVWEFSHV FT HYCNKNRKPHPTQKPEALYERMILASSNEGDIVLDPFVGSGTLNFVCKHLNRSGIGIDI FT NKEYIEMAKERLDSEFNGFDSIDERMKRCPNDLSDPVIRKQYIINHINWFLKNHENARE FT EFLNEVKTKYYKKMTQEEKRLLDKENNLSFDFS" XX SQ Sequence 2492 BP; 969 A; 385 C; 408 G; 730 T; 0 other; gaattcgaca accaaggttt aatcaatgaa aactatgtcg gcatccgccc tgcaccaggt 60 tatccaagct gcccagaaca tactgaaaaa tccttgattt gggatttatt agaagtggaa 120 cagcgcatcg gtatgaaact caccgaaagc tacgccatgt ggccggcggc ttccgtctgt 180 ggttggtact tcacccatcc agcaagtaac tatttcactt taggtcgcat tgatgaagac 240 caagctcaag attatgccaa acgtaaaggc tgggatgaga gagagatgat gaaatggttg 300 ggtgtggcga tgaagtaaag gagagaatat aaaattagaa atttaagtta ataaataagg 360 aaacctcatg aaatacgaag aaattaattt taaagtgcct gtagagtccc cttattaccc 420 aaattattct caatgtgtaa tagaaagaat atattccatt cttcgaaatc aaaaagatat 480 gggagatgat agaataatta ttaatactaa tttaaagaaa ggacttccat tagaaaatat 540 aaataaaata gcaggaccta tgattgaagc ttgggctgaa gaagtttttt caggaattag 600 agataataga gataaccaat ataatctaat taatgttgaa gctcaagaaa gattaggaat 660 ttctgatata atacttcaat tccaagttaa taataatgta attacaggaa atgtggatgt 720 aaaagcaaca tcaaatgata ttcctgatag tgggaaaagc cctaatatca caagctttag 780 tcgaattaga actgcatatg ttaaagaccc taattttatt tttataatat tatccataaa 840 acacagtgta tatgtaaaaa gaaatgaata cacaaattta atggatggaa taatgcaaat 900 tattgacttt aatgtttatg atttaaagta tatatctgat tcagacataa gttataaccc 960 agcattagga actggtcaaa ttcaaataaa ggatattcat tatgtttcct ctcaaaaaag 1020 aacaacctgg caaatgtgcc agttattaga tttaaaatac ttaagaagca aaaaaagaac 1080 tatagaacaa ttctataatg aggcaaaaag aaataaatgg atcaaagatt aatttgcagc 1140 aatgctataa aagcattaaa aaatttagaa gaaaattcta tagatttaat tataacagat 1200 cctccatata atttggggaa agactatggg actactgacg ataatttaaa ttttaataaa 1260 tacttagaat ttagtcatga atggctagaa gaatgttata gagttctaaa accacatgga 1320 acaatatata tatttatggg aatgaaatat atatcttata tatataaaat attagagcaa 1380 gatttaggta tgtacttcaa tagttggatc acttggtatt acacgcaagg aatcggaaaa 1440 actagaggat attctcctag gcatgatgat attctaatgt ttacaaaaca tcctaaaaaa 1500 tttactttca atttggatcg tattagagtt cctcaaaagt attatagaag tgtaaataat 1560 atgagaggtg caaatcctag taatgtttgg gaattttctc acgtccatta ttgcaataaa 1620 aatcgaaagc ctcatccaac tcaaaaacca gaggctttat atgaaagaat gatactcgct 1680 tcatcaaatg aaggagacat tgtattggac cctttcgttg gttctggaac actaaatttt 1740 gtttgtaaac atctcaaccg ttcgggaatc ggtattgata taaataaaga atatattgaa 1800 atggccaaag aacggctaga ttctgaattt aatggttttg atagtataga cgaaagaatg 1860 aagcgatgtc ctaatgactt aagtgatcca gtaataagaa aacaatatat tataaatcat 1920 attaattggt ttctaaaaaa tcatgaaaac gctagagaag aatttttaaa tgaagtaaag 1980 acgaaatatt ataaaaaaat gactcaagaa gaaaaaaggc tacttgataa agaaaataac 2040 ttatcttttg atttttctta aagagagtct caacaaaagc acaagtactt tatttacact 2100 tgtgctatct ttcttatttg caacattaca ccgctataca atgcatatca cagtatgcac 2160 ctagcttcaa caaaacagct aaaaatcaac cgcactttca cttccaaact gcgcataact 2220 aatcccatct cttgcacaat ccccaatttt cgctatcaca ttccctttct cattcactct 2280 actctcggaa ctttatcaat gaataaattt aaaatagcat tactcacggc tgcattcgta 2340 gggttgtttg cttgtaccac aacacaacaa ccaaagaaaa ccgttaaaac acgatacctt 2400 aaaaataaca ttagccaagc ggaattaaac aatccgaaag attacaagcg ttattactat 2460 tcttgccgta attcagaaac gggctcgaat tc 2492 // |
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