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EBI DbfetchID D10387; SV 1; linear; genomic DNA; STD; PRO; 6190 BP. XX AC D10387; XX DT 20-JUL-1992 (Rel. 32, Created) DT 13-MAY-2009 (Rel. 100, Last updated, Version 11) XX DE Serratia marcescens genes for leader peptide, aspartokinase I-homoserine DE dehydrogenase I, homoserine kinase, threonine synthase, complete cds. XX KW . XX OS Serratia marcescens OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Serratia. XX RN [1] RP 1-6190 RA Omori K.; RT ; RL Submitted (27-JAN-1992) to the EMBL/GenBank/DDBJ databases. RL Contact:Kenji Omori Tanabe Seiyaku, Co. Ltd, Lead Generation Research RL Laboratory; 16-89, Kashima-3-chome, Yodogawa-ku, Osaka, Osaka 532, Japan XX RN [2] RP 1-6190 RX PUBMED; 8423151. RA Omori K., Imai Y., Suzuki S., Komatsubara S.; RT "Nucleotide sequence of the Serratia marcescens threonine operon and RT analysis of the threonine operon mutations which alter feedback inhibition RT of both aspartokinase I and homoserine dehydrogenase I"; RL J. Bacteriol. 175(3):785-794(1993). XX DR RFAM; RF00506; Thr_leader. XX FH Key Location/Qualifiers FH FT source 1..6190 FT /organism="Serratia marcescens" FT /strain="Sr41" FT /isolate="TLr156" FT /mol_type="genomic DNA" FT /clone="pSK301" FT /db_xref="taxon:615" FT -35_signal 736..741 FT -10_signal 757..762 FT misc_feature 769..773 FT /note="transcription start site" FT RBS 788..791 FT CDS 804..872 FT /codon_start=1 FT /transl_table=11 FT /product="leader peptide" FT /function="attenuation control" FT /db_xref="GOA:Q04208" FT /db_xref="InterPro:IPR011720" FT /db_xref="UniProtKB/Swiss-Prot:Q04208" FT /protein_id="BAA38479.1" FT /translation="MRNISLTTTIITTTDTTGNGAG" FT repeat_region 861..905 FT /rpt_type=INVERTED FT /rpt_unit_range=896..905 FT /rpt_unit_range=861..870 FT repeat_region 891..925 FT /rpt_type=INVERTED FT /rpt_unit_range=891..904 FT /rpt_unit_range=912..925 FT RBS 940..943 FT CDS 953..3412 FT /codon_start=1 FT /transl_table=11 FT /gene="thrA1-5/A2-5/BC" FT /product="aspartokinase I-homoserine dehydrogenase I" FT /note="fused-type enzyme" FT /db_xref="GOA:P27725" FT /db_xref="HSSP:1EBF" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:P27725" FT /protein_id="BAA38480.1" FT /translation="MRVLKFGGTSVANAERFLRVADIMESNARQGQVATVLSAPAKITN FT HLVAMIDKTVAGQDILPNMSDAERIFADLLSGLAQALPGFEYDRLKGVVDQEFAQLKQV FT LHGVSLLGQCPDSVNAAIICRGEKLSIAIMEGVFRAKGYPVTVINPVEKLLAQGHYLES FT TVDIAESTLRIAAAAIPADHIVLMAGFTAGNDKGELVVLGRNGSDYSAAVLAACLRADC FT CEIWTDVDGVYTCDPRTVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCL FT IKNTSNPQAPGTLIGKDSTDADMPVKGITNLNNMAMINVSGPGMKGMVGMAARVFAVMS FT RAGISVVLITQFSSEYSISFCVPQGELQRARRALEEEFYLELKDGVLDPLDVMERLAII FT SVVGDGMRTLRGISARFFSALARANINIVAIAQGSSERSISVVVSNDSATTGVRVSHQM FT LFNTDQVIEVFVIGVGGVGGALIEQIYRQQPWLKQKHIDLRVCGIANSRVMLTNVHGIA FT LDSWRDALAGAQEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYVDFLADGFHVVT FT PNKKANTSSMNYYQQLRAAAAGSHRKFLYDTNVGAGLPVIENLQNLLNAGDELVRFSGI FT LSGSLSFIFGKLDEGLSLSAATLQARANGYTEPDPRDDLSGMDVARKLLILAREAGYKL FT ELSDIEVEPVLPPSFDASGDVDTFLARLPELDKEFARNVANAAEQGKVLRYVGLIDEGR FT CKVRIEAVDGNDPLYKVKNGENALAFYSRYYQPLPLVLRGYGAGNDVTAAGVFADLLRT FT LSWKLGV" FT variation 1414 FT /replace="g" FT /note="wild type" FT variation 2007 FT /replace="c" FT /note="wild type" FT variation 2390 FT /replace="c" FT /note="wild type" FT variation 2423 FT /replace="c" FT /note="wild type" FT RBS 3404..3407 FT CDS 3414..4343 FT /codon_start=1 FT /transl_table=11 FT /product="homoserine kinase" FT /EC_number="2.7.1.39" FT /db_xref="GOA:P27722" FT /db_xref="InterPro:IPR014721" FT /db_xref="UniProtKB/Swiss-Prot:P27722" FT /protein_id="BAA38482.1" FT /translation="MVKVYAPASIGNVSVGFDVLGAAVSPIDGTLLGDCVSVEAAETFS FT LQNAGRFVSKLPAEPKENIVYQCWERFCQEIGREVPVAMRLEKNMPIGSGLGSSACSVV FT AGLMAMNEFCDRPLDKTTLLGLMGELEGRISGSVHYDNVAPCYLGGLQLMLEEEGIISQ FT EVPCFDDWLWVMAYPGIKVSTAEARAILPAQYRRQDCISHGRYLAGFIHACHTRQPQLA FT AKLMQDVIAEPYRTRLLPGFAEARKAAQEIGALACGISGSGPTLFAVCNDGATAQRMAA FT WLQQHYLQNDEGFVHICRLDTAGARLLG" FT RBS 4338..4340 FT CDS 4347..5636 FT /codon_start=1 FT /transl_table=11 FT /product="threonine synthase" FT /EC_number="4.2.3.1" FT /db_xref="GOA:P27735" FT /db_xref="HSSP:1KL7" FT /db_xref="InterPro:IPR004450" FT /db_xref="UniProtKB/Swiss-Prot:P27735" FT /protein_id="BAA01222.1" FT /translation="MKLYNLKDHNEQVSFAQAIKQGLGKQQGLFFPLDLPEFELTEIDH FT LLEQDFVTRSSRILSAFIGEEVPETALKKRVQAAFEFPAPVAKVTDDVSCLELFHGPTL FT AFKDFGGRFMAQMLAEVAGEQPVTILTATSGDTGAAVAHAFYGLKNVRVVILYPQGKIS FT PLQEKLFCTLGGNIHTVAIDGDFDACQALVKQAFDDQELKDALHLNSANSINISRLLAQ FT ICYYFEAVAQLPQEARNQLVISVPSGNFGDLTAGLLAKSLGLPVKRFIAATNANDTVPR FT FLTSGQWQPHATVATLSNAMDVSQPNNWPRVEELFRRKVWQLKELGHAAVSDETTKDTM FT RELAELGYISEPHAAIAYRALRDQLQEGEFGLFLGTAHPAKFKESVEAILGQELPLPKA FT LALRAELPLLSHTLPASFGELRKFLMGLPA" FT stem_loop 5658..5681 FT /note="pot. transcription terminator" XX SQ Sequence 6190 BP; 1191 A; 1830 C; 1952 G; 1217 T; 0 other; gtcgacagcg aggcgcatct gcagccggcg gcgcgctggg tggcacatgg ggccggcgag 60 atcctcgacg gcgtgcaaac gttcgccacg ctggaacagg cgctggcgga tgtggatttt 120 accgtcgcca ccaccgcccg cagtcgcgcg cgttttcact actactgcac gccgcctgag 180 ctgctggaac agctgagtga gcgtaagcag tgggtcggcc aggccgcgct ggtattcggc 240 cgcgaggatt ccggcctgac caacgaagag ctggcgctgg cggatttgct gaccggcgtg 300 ccgatgcagg cggattatcc gtcgctcaat ctgggccagg cggtgatggt gtactgctac 360 cagttggcga gcctgatggg cgtcaacgcc gcgccgcagg aggctgcggc gcctgaaggg 420 caactcaggg cgttgcgcca ccgcgccgat gctttgttgg atgccctgga ggtcggcgat 480 gaccaaaaat tgcgcgactg gctgcaccag cgcctcggtg cgttgccgca gcgagatacg 540 gcgatgctgc atacgctgct gcacgacatc gaaaaaaaac tcgccaagtg atagcggctc 600 cagcaggcgg ttactcagtg tgatccacta ttaaaccggc tttaggttgt ttttttgttc 660 tgtcattttg cttttcacct gttgcatcgc gcattaccgc cgctgaacgg aaaataagca 720 aaaataagaa atcgtttgac ttgggatagc gattgcttta accaatagag gggacagagt 780 atttcatgag acagacagac aacatgcgca atatcagcct gactaccaca attattacca 840 ccaccgatac cacaggtaac ggggcgggct gacgcgcaca ggaaacacag aaaaaagccc 900 gcacctaatc agtgcgggct tttttttcgg cttaaattcg gagagatcac aaatgcgagt 960 gctgaaattt ggcggaacct cggtagcgaa tgcggaacgt tttctgcgcg tcgccgacat 1020 catggagagt aacgcgcgtc agggacaggt agccacggtc ttgtccgccc ccgcaaagat 1080 caccaaccat ctggtggcga tgatcgacaa aacggtggcg ggccaggaca ttctgccgaa 1140 tatgagcgac gccgagcgga tctttgccga cctgctgagc ggactggcgc aggcgctgcc 1200 gggctttgaa tacgatcgtt tgaaaggcgt ggtcgatcag gaattcgcgc agctcaaaca 1260 ggtgctgcat ggcgtctcgc tgctggggca gtgcccggac agcgtgaacg cggcgatcat 1320 ctgccgtggc gaaaagctct ccatcgccat catggaaggg gtgttccgcg ccaagggtta 1380 tccggtcacg gtgatcaacc cggtggagaa actactggcg cagggccact acctggagtc 1440 caccgtggac atcgccgagt ctacgctgcg catcgccgcc gcggcgatcc cggccgatca 1500 catcgtgctg atggccggtt tcaccgccgg taatgataag ggcgagctgg tggtgctggg 1560 ccgcaacggc tccgactatt ccgccgcggt gctggccgcc tgcctgcgcg ccgactgttg 1620 cgagatctgg accgacgtcg acggcgttta tacctgcgat ccgcgcaccg tgccggacgc 1680 caggttactg aaatcgatgt cgtaccagga agcgatggag ctttcctatt tcggcgccaa 1740 agtgttacac ccccgcacca tcactccgat tgcccagttt caaatccctt gcctgattaa 1800 aaacacctcc aacccgcagg cccccggcac gctgatcggc aaagacagca ccgatgccga 1860 tatgccggtg aagggcatca ccaacctgaa caacatggcg atgatcaacg tctccggccc 1920 gggcatgaaa ggcatggtcg gcatggccgc acgggtcttc gccgtgatgt cgcgcgccgg 1980 catttcggtg gtgctgatca cccaattctc ttccgaatac agcatcagct tctgcgtgcc 2040 gcagggtgaa ctgcagcgcg cccgtcgcgc gctggaagag gagttctatc tggagctgaa 2100 agacggcgtg ctggatccgc tggacgtgat ggaacgcctg gcgatcatct cggtggttgg 2160 cgacggcatg cgcaccctgc gcggcatttc cgcacgcttc ttctccgcgc tggcgcgcgc 2220 caacatcaat atcgttgcca tcgcccaagg gtcttccgaa cgttccattt cggtggtggt 2280 cagcaatgat tccgccacca ccggcgtgcg cgtcagccac cagatgctgt tcaacaccga 2340 tcaggtgatc gaagtgttcg tcatcggcgt cggcggcgtc ggcggggcgt tgatcgagca 2400 gatctatcgc cagcagccgt ggttgaagca aaaacatatc gatctgcggg tgtgcggcat 2460 cgccaactcg cgcgtgatgc tgaccaatgt gcacggcatc gcgctggaca gctggcgcga 2520 cgcgctggcc ggcgcgcagg agccgttcaa tctcggccgt ctgatccggc tggtgaagga 2580 atatcacctg ctgaacccgg tgatcgtcga ctgtacctcc agtcaggcgg tggccgatca 2640 gtacgtggac ttcctggcgg acggcttcca tgtggtgacg ccgaacaaaa aggccaacac 2700 ctcgtcgatg aactattatc agcaactgcg cgcggccgcc gccggttcgc atcgcaagtt 2760 cctgtacgac accaacgtcg gtgccggtct gccggtgatt gagaacctgc aaaacctgct 2820 gaacgccggt gatgaactgg tacgtttctc cggtatcctg tccggctcgc tgtcctttat 2880 cttcggcaag ctggacgaag ggctgtcgct gtcggcggcg accctgcagg cccgggccaa 2940 cggctacacc gagccggatc cgcgcgacga tctgtccggg atggatgtgg cgcgtaagct 3000 gctgatcctg gcgcgcgagg ccggttacaa actggaactg agcgatatcg aggtcgagcc 3060 ggtgctgccg ccgtccttcg acgcgtcggg cgacgtggac accttcctgg cgcggttgcc 3120 ggagctggat aaagagttcg cgcgcaacgt ggctaacgcc gccgagcaag gcaaggtgct 3180 gcgctatgtc ggcctgatcg acgaagggcg ctgcaaggtg cgcattgagg cggtggacgg 3240 caacgatccg ttgtataaag tgaagaacgg cgagaacgcg ctggccttct acagccgcta 3300 ctatcagccg ttgccgctgg tgctgcgcgg ctacggcgcc ggtaacgatg tgactgcagc 3360 gggcgtattc gccgatctgc tgcgcacact gtcatggaag ttgggagttt aatatggtta 3420 aggtgtatgc accggcctcg atcggcaacg tcagcgtcgg tttcgacgtg ctgggcgcgg 3480 cggtttcgcc gatcgacggc acgctgctgg gcgactgtgt cagcgtcgaa gccgccgaga 3540 cgttcagttt gcagaatgcc gggcgcttcg tcagcaagct gccggccgag ccgaaagaga 3600 acatcgttta tcaatgctgg gagcgcttct gccaggagat cggccgcgaa gtgccggtgg 3660 cgatgcgttt ggaaaagaac atgccgatcg gatcggggct ggggtccagc gcctgttcgg 3720 tggtcgccgg gctgatggcg atgaacgaat tctgcgatcg tccgttggat aagacgacgc 3780 tgctcggcct gatgggcgag ctggaagggc gcatttccgg cagcgtgcat tacgacaacg 3840 tggcgccttg ctatctgggc ggcctgcagc tgatgctgga agaagaaggc attatcagcc 3900 aggaggtgcc gtgctttgac gattggctgt gggttatggc ctatccgggc atcaaagtgt 3960 ccaccgccga agcgcgcgcc attttacccg cgcaataccg tcgtcaggat tgcatcagcc 4020 atggccgcta tctggccggc tttattcacg cctgccatac ccggcagccg cagctggccg 4080 ccaaattgat gcaggacgtg atcgccgaac cgtaccgcac ccggctgttg ccgggcttcg 4140 ccgaagcgcg caaggcggcg caggagatcg gcgcgttggc ctgcggcatc tccggctctg 4200 gcccgacgct gttcgccgtc tgcaatgacg gcgccaccgc gcaacgcatg gccgcctggc 4260 tgcaacaaca ctatctgcaa aacgacgaag gttttgttca tatttgccgt ctggataccg 4320 caggcgctcg actactggga taacgcatga aactgtacaa ccttaaggat cacaacgagc 4380 aggtcagctt cgcgcaggcg atcaaacaag gcctgggcaa gcaacagggg ctgttcttcc 4440 cgctggatct gccggagttc gaactgaccg aaatcgacca cctgctggag caggattttg 4500 tcacccgcag cagccgcatt ttgtcggcct ttatcggcga agaggtgccg gaaacggcgc 4560 tgaagaaacg cgtgcaggcc gcctttgagt tcccggcgcc ggtggcgaaa gtgaccgatg 4620 acgtctcctg cctggagctg ttccacggcc cgacgctggc gttcaaagac ttcggcggcc 4680 gctttatggc gcagatgctg gcggaagtgg ccggcgagca gccggtgacc attctgaccg 4740 ccacctccgg cgacaccggc gccgccgtgg cgcacgcctt ctacggcctg aaaaacgtgc 4800 gggtggtgat cctgtatccg cagggcaaaa tcagcccgct gcaggaaaag ctgttctgca 4860 ccctgggcgg caacattcac accgtggcga tcgacggtga cttcgatgct tgtcaggcgc 4920 tggtgaagca ggcgttcgac gatcaggaat tgaaagacgc gctgcacctg aactcggcca 4980 actcgattaa catcagccgt ttgctggcgc agatttgcta ctacttcgaa gcggtggcgc 5040 agctgccgca ggaagcgcgc aatcagctgg tgatttcggt gccgagcggc aacttcggcg 5100 atctgaccgc cgggctgctg gccaaatcgc tgggcctgcc ggtgaagcgc ttcatcgccg 5160 ccaccaacgc caacgacacc gtgccgcgct tcctgaccag cggccagtgg cagccgcacg 5220 ctacggtcgc gacgctgtcc aacgccatgg acgtcagcca gccgaacaac tggccgcgcg 5280 tggaagagct gttccgtcgc aaggtctggc agttgaagga gctgggtcac gcggcggtca 5340 gcgacgagac caccaaagac acgatgcgtg agctggccga gctgggctat atctccgaac 5400 cgcatgcggc catcgcctac cgcgcgctgc gtgatcagtt gcaggaaggg gagttcggcc 5460 tgttcctcgg caccgcgcat ccggccaagt ttaaagagag tgtggaagcg atactcggcc 5520 aggagttgcc gttgccgaaa gcgctggcgc tgcgggctga gctgccgctg ctgtcgcaca 5580 cgctgccggc cagcttcggc gagctgcgca agttcctgat ggggctgcct gcctgaagtt 5640 cggcaggcta aaatgcgaaa agccgtcttg cgacggcttt tttttatgcc tgctccgggc 5700 gtttgaacac cagttcattg ccttgcgagg cggcttcgtc gaaggcgtag ccttccaggt 5760 tgaagtccgc cagctgctcg ctgcgcgtca ggcggttttt gatgataaag cggctcatca 5820 acccgcgcgc tttcttggcg tagaagctga tgaccttgaa cttgccgttc ttctcgtcga 5880 ggaacaccgg tttgatcagc gagccgtgca gcttggccgg cttcaccgct ttgaaatact 5940 cgtctgacgc caggttgacc accacgtcgt cgccctgttg ctccagcgct tcgttgagct 6000 ttttggtgat ctgatcgccc cagaagctgt acagatcttt gcccttcggg ttttccagct 6060 tgatgcccat ctccagccgg tacggcatca tcagatcgag cgggcgcagt acgccataca 6120 ggccggacag catgcgcaga tgctgctggg cgaagtcgaa atcggcttcg ttgaagtcct 6180 gcgcctgcag 6190 // |
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