EBI Dbfetch

ID   D10358; SV 1; linear; genomic DNA; STD; PRO; 1654 BP.
XX
AC   D10358;
XX
DT   12-MAY-1992 (Rel. 31, Created)
DT   07-DEC-2007 (Rel. 94, Last updated, Version 8)
XX
DE   Klebsiella aerogenes folA and apaH genes.
XX
KW   diadenosine tetraphosphatase; dihydrofolate reductase.
XX
OS   Klebsiella aerogenes
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Klebsiella.
XX
RN   [1]
RP   1-1654
RX   PUBMED; 1551851.
RA   Azakami H., Sugino H., Murooka Y.;
RT   "Cloning and nucleotide sequence of a negative regulator gene for
RT   Klebsiella aerogenes arylsulfatase synthesis and identification of the gene
RT   as folA";
RL   J. Bacteriol. 174(7):2344-2351(1992).
XX
CC   These data kindly submitted in computer readable form by:
CC   Yoshikatsu Murooka
CC   Department of Fermentation Technology
CC   Faculty of Engineering, Hiroshima University
CC   1-4-1 Kagamiyama
CC   Higashi-Hiroshima 724
CC   Japan
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1654
FT                   /organism="Klebsiella aerogenes"
FT                   /strain="W70"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:28451"
FT   -35_signal      84..89
FT   -10_signal      107..112
FT   RBS             133..137
FT   CDS             145..624
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="folA"
FT                   /product="dihydrofolate reductase"
FT                   /db_xref="GOA:P27498"
FT                   /db_xref="HSSP:1DHI"
FT                   /db_xref="InterPro:IPR017925"
FT                   /db_xref="UniProtKB/Swiss-Prot:P27498"
FT                   /protein_id="BAA01187.1"
FT                   /translation="MISLIAALRVDRVIGMENAMPWNLNEDLAWFKRNTLNKPVVMGRL
FT                   TWESIGRPLPGRKNIVISSKPGSDDRVQWVSSVEEAIAACGDVEEIMVIGGGRVYDEFL
FT                   PKAQKLYLTHIDAEVEGDTHFPDYDPDEWESVFSEFHDADAQNSHSYCFEILERR"
FT   CDS             complement(875..>1654)
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="apaH"
FT                   /product="diadenosine tetraphosphatase"
FT                   /db_xref="GOA:P27510"
FT                   /db_xref="InterPro:IPR004843"
FT                   /db_xref="UniProtKB/Swiss-Prot:P27510"
FT                   /protein_id="BAA01188.1"
FT                   /translation="EFDPRRDTLWLTGDLVARGPGSLEVLRYVKSLGDTVRLVLGNHDL
FT                   HLLAVFAGISRNKPKDRLKPLLEAPDADELLNWLRRQPLLQVDEEKKLVMAHAGITPQW
FT                   DLETAQQCARDVEAVLSSDSYPFFLDAMYGDMPNHWSNELSGLARLRFISNAFTRMRYC
FT                   FPNGQLDMYSKEAPEDAPAPLKPWFAIPGPVSNAYSIAFGHWASLEGRGTPEGIYALDT
FT                   GCCWGGELTCLRWEDKQYFTQPSNRQKSLDEGEAVAS"
XX
SQ   Sequence 1654 BP; 342 A; 467 C; 504 G; 341 T; 0 other;
     ggatcctctt ctggcggccc acggtgtgaa cgtgggcttg ccagcaaatc agaaaatttt        60
     cttattcttt accctccggc cagtcgacga agttgtacgc tttccgtata gtggcgacaa       120
     ttttttgcac tcgggaatga atgaatgatc agtctgattg cggcgttacg ggtagatcgc       180
     gtcattggta tggaaaacgc catgccatgg aacctgaatg aagatctcgc ctggttcaaa       240
     cgcaacacgt taaataagcc ggtggtgatg gggcgtctga cctgggaatc cattgggcgc       300
     ccattgccgg ggcgtaaaaa tatcgtgatc agcagcaaac ccggcagcga cgatcgcgtg       360
     cagtgggtct cctccgtaga agaagcaatt gccgcctgcg gcgatgtgga agagattatg       420
     gtgatcggcg gcggccgggt gtatgacgag ttcctgccga aagcgcagaa gctctacttg       480
     acccatattg atgcggaagt ggaaggcgat acccatttcc cggattacga tccggacgaa       540
     tgggagtcgg tgttcagcga gtttcatgac gccgacgcgc agaactccca tagctactgc       600
     tttgagatcc tcgaacgccg ttaagcgggc aggatagcga taaaaaaacc ccggtcgcgt       660
     ctggcgtgac cggggttttc ttttgcatga attgcgcgac gatggcgcac tcagtatctc       720
     gtcgtgtttc ccgtcgcggg cttcgtcttc ctggagcgct gcccgggcta caaatggcaa       780
     ggaaatgtag gtcgggtaag gcgttagcgc cacccgacaa aacgacgccg caggtagccc       840
     ggataaggcg caacgcgccg ctatccggga agagtcagga cgcgacggct tcgccttcgt       900
     cgaggctttt ctgccggttt gacggctggg tgaagtactg cttatcttcc cagcgcaggc       960
     aggtgagttc cccgccccag cagcagccgg tatccagggc gtaaatcccc tccggcgtgc      1020
     cgcgtccctc cagcgaagcc cagtggccaa aggcaatgct gtaggcattg ctgaccgggc      1080
     cgggaatggc aaaccacggc ttcagcggcg ccggggcgtc ctcgggcgct tccttcgaat      1140
     acatatccag ctggccgttc gggaaacagt agcgcatgcg ggtaaaggcg ttagagataa      1200
     agcgcaggcg cgccaggccg ctcagctcgt tgctccagtg gttcggcatg tcgccgtaca      1260
     tggcatcgag gaagaaagga taggagtcgc tggagagcac tgcttcaaca tcgcgcgcgc      1320
     actgctgggc ggtctccaga tcccactgcg gcgtaatgcc ggcatgggcc atcaccagct      1380
     ttttctcttc atccacctgc agcagcggct ggcgacgcag ccagttgagc agctcatcgg      1440
     catccggcgc ttccagcagt ggtttcagac gatcctttgg cttgttgcgg ctgataccgg      1500
     caaagaccgc cagcaggtgt aagtcatggt tgcccagcac cagtcgtacc gtgtcgccca      1560
     gagatttgac atagcgcagg acctccagcg atcccgggcc tcgggccacc agatcgccgg      1620
     tcagccacag cgtatctctc cgcggatcga attc                                  1654
//