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EBI DbfetchID D00704; SV 1; linear; genomic DNA; STD; PRO; 3106 BP. XX AC D00704; XX DT 17-JUL-1991 (Rel. 28, Created) DT 18-NOV-2009 (Rel. 102, Last updated, Version 5) XX DE Aneurinibacillus aneurinilyticus genes for BanI restriction endonuclease, DE BanI modification methylase, complete cds. XX KW . XX OS Aneurinibacillus aneurinilyticus OC Bacteria; Firmicutes; Bacillales; Paenibacillaceae; Aneurinibacillus group; OC Aneurinibacillus. XX RN [1] RX PUBMED; 2358438. RA Maekawa Y., Yasukawa H., Kawakami B.; RT "Cloning and nucleotide sequences of the BanI restriction-modification RT genes in Bacillus aneurinolyticus"; RL J. Biochem. 107(4):645-649(1990). XX DR StrainInfo; 33950; IAM 1077. XX CC BanI methlase contains sequences common to cytosine-specific CC methylasea. XX FH Key Location/Qualifiers FH FT source 1..3106 FT /organism="Aneurinibacillus aneurinilyticus" FT /strain="IAM 1077" FT /mol_type="genomic DNA" FT /clone="pBanIRM8" FT /note="1625 bp upstream of EcoRI site." FT /note="synonym: Bacillus aneurinolyticus" FT /db_xref="taxon:1391" FT RBS 491..495 FT /note="ribosome binding sequence of BanI restriction gene" FT CDS 502..1566 FT /codon_start=1 FT /transl_table=11 FT /product="BanI restriction endonuclease" FT /db_xref="GOA:P19887" FT /db_xref="UniProtKB/Swiss-Prot:P19887" FT /protein_id="BAA00612.1" FT /translation="MAQLKYNKDIDELERNAAKWWPDFLAKKESSTSIIPKLVESQDAF FT ISLLNLSKNNPFDIFQLIDASKFPPNLFLKHLVVLTDFGGEPLNRLNQNFDSLFPMIPY FT GIHYITKVLGKFEFFWNEKKYEYVFQELPVTSLTNSKLKIDGASISKTVPLSDLYKDVI FT VLLMFGANAVNSEVSEVLMKCEVGNLIGKTDELKKFIKERYIFVSRITGGAEANTLGQV FT AQTHVIDFLRTRFGSKGHDIKSNGHIEGVTHNDGQTLTTFDVVIKKGSKSVAIEISFQV FT TTNSTIERKAGQAKARYDMVSDTGNYIAYIIDGAGNFQRKNAITTICNNSHCTVAYTEE FT ELNVLLKFILEKLE" FT RBS 1576..1579 FT /note="ribosome binding sequence of BanI modification gene" FT CDS 1585..2871 FT /codon_start=1 FT /transl_table=11 FT /product="BanI modification methylase" FT /db_xref="GOA:P19888" FT /db_xref="InterPro:IPR001525" FT /db_xref="InterPro:IPR018117" FT /db_xref="UniProtKB/Swiss-Prot:P19888" FT /protein_id="BAA00613.1" FT /translation="MKIKFVDLFAGIGGIRIGFERAAKRFELETECVLSSEIDKKACET FT YALNFKEEPQGDIHEITSFPEFDFLLAGFPCQPFSYAGKQQGFGDTRGTLFFEVERVLR FT DNRPKAFLLENVRGLVTHDKGRTLKTIISKLEELGYGVSYLLLNSSTFGVPQNRVRIYI FT LGILGSKPKLTLTSNVGAADSHKYKNEQISLFDESYATVKDILEDSPSEKYRCSDEFIG FT QLSKVVGNNFELLHGYRLIDYRGGNSIHSWELGIKGDCTKEEIEFLNQLIANRRKKIYG FT THQDGKALTLEQIRTFYNHDQLEVIIKSLLQKGYLREEENKFNPVCGNMSFEVFKFLDP FT DSISITLTSSDAHKLGVVQNNVPRRITPRECARLQGFPDDFILHSNDNFAYKQLGNSVT FT VKVVEKVIEDLFQNNVNELFGQMKLANVV" FT terminator 2881..2903 FT /note="transcritional termination signal" XX SQ Sequence 3106 BP; 1029 A; 523 C; 640 G; 914 T; 0 other; atcaaatgca gatgttatcg tcgtggaccc gccgcgcaaa ggctgtgatg agcggttgtt 60 ggacaccatt gtagccatga agcccgagcg cgtagtatat gtatcatgta atccggctac 120 cctggcgcgc gacctgcgta ttctggaaga tgggggttac aggacgatag aggtgcagcc 180 gtggacatgt ttccccatac gaatcatgtg gagtgctgtt cactgttggt caggaatgaa 240 aactaatatg gagatatatt ggcgaaaatc aaaaaggagc gggtaatgct actgatcggt 300 agccccgctt ttatttttgt gcgcacaact attcaattaa agtagggtac ggatttaaca 360 acacttacac tggaaatcag aaactatttt ttgtgaatcg acaccccgtg tcctctataa 420 attattgcta gtgtgtcgaa acaagagtaa tgcgacaaaa aatgatataa tatagaaaaa 480 ctttggaggt gaggatataa gatggcacag cttaaataca ataaagatat tgatgaattg 540 gaaagaaacg ctgcaaagtg gtggccggat tttctagcta aaaaggagtc gtcaacgagc 600 attattccta aactggtcga atcgcaagat gcttttatat ctttattaaa tctcagtaag 660 aacaaccctt tcgacatttt ccaactaata gacgcatcta agttcccacc caatctgttt 720 ctaaagcatc ttgttgtatt aaccgacttt ggtggagagc cactaaatcg cttgaatcaa 780 aactttgatt cgctttttcc catgattccc tatggaatcc attacataac aaaagtgtta 840 gggaaatttg aatttttttg gaatgagaaa aaatatgaat atgtttttca agaacttccg 900 gttacatcat taactaattc aaaactcaag attgacggag caagtatttc taagacagtc 960 ccactttctg atttatataa agatgtaatt gtattactca tgttcggtgc taatgctgtt 1020 aattcggaag tatccgaagt tttgatgaaa tgtgaagttg gaaatctcat tggtaagact 1080 gacgagttga agaagtttat taaggagcgt tatatttttg taagcagaat aacaggaggg 1140 gctgaagcaa atacgttagg tcaagtcgct caaacccatg taatagattt tctaagaact 1200 cgattcgggt ctaagggtca cgatataaaa agtaatggac acattgaagg tgtgacccat 1260 aacgatggac aaacactaac cacttttgat gtagtcataa aaaaaggcag caaaagcgtt 1320 gcgattgaga ttagcttcca ggtaactaca aatagtacca tcgagcgtaa agctggacaa 1380 gctaaagcac gatacgatat ggttagtgat actggcaatt atatagctta tattatcgat 1440 ggagcgggga acttccaacg taaaaatgca atcacaacca tttgtaataa tagccattgc 1500 acagtagctt ataccgaaga agaactaaat gtcttgctaa aattcatctt agaaaaactt 1560 gaatagttat ttggggaggc aactatgaag attaaatttg ttgacttgtt tgctggaatt 1620 ggaggaattc gaataggatt tgaaagagcg gccaaacggt ttgaattgga gactgagtgt 1680 gtactttcta gtgagatcga caaaaaggct tgcgaaacat atgcccttaa ttttaaagag 1740 gaacctcaag gtgatataca cgaaattacc agctttccag aattcgattt tcttcttgct 1800 ggatttcctt gtcaaccctt ttcatatgca ggtaaacaac aaggatttgg ggatacaaga 1860 ggaacattat tttttgaggt ggagagggtt cttagggata accgcccaaa ggctttctta 1920 ttagaaaatg ttcggggact ggtaacacac gacaaaggaa gaactttaaa gacgatcatt 1980 tcaaaattag aggaattggg ttacggggtt agctacctat tacttaacag tagtacgttt 2040 ggtgtccccc aaaaccgagt aaggatttat attttaggca tactcggaag taaaccaaag 2100 ttaactctaa cctctaatgt tggagcagcc gattctcaca agtataagaa tgaacaaata 2160 tctttatttg atgaaagcta tgctactgtt aaagacattt tagaagattc tccaagtgaa 2220 aaatatcgct gttctgacga atttatcggt caactttcaa aggttgttgg taataacttt 2280 gaattgctac atggctatcg cttaattgat tatcgtggcg gtaattcgat tcattcttgg 2340 gaacttggca tcaagggtga ttgtacaaaa gaggaaattg aatttctaaa tcagttgatt 2400 gcaaatagaa gaaagaaaat ttatggtacg caccaagacg gaaaagcctt aactttagag 2460 caaattcgga cgttttataa ccacgatcag cttgaggtaa taataaaatc cctactccaa 2520 aaaggatatt taagagaaga agaaaataaa tttaatcccg tttgtgggaa tatgtctttt 2580 gaggtcttta agttccttga cccagacagt atttcaatca cactcacatc tagcgatgct 2640 cataagttgg gagtagttca gaataatgtt cctagaagga taacacctcg ggaatgtgct 2700 agactgcaag gtttcccaga tgatttcata ctacattcca atgacaattt tgcatacaag 2760 cagttgggga attcagtcac tgtcaaagta gtggaaaaag tgatcgagga tttatttcaa 2820 aacaatgtaa atgaattgtt tggacagatg aaacttgcaa atgttgtatg aatataaatt 2880 aaccccacaa ttattgtggg gttgtttatg tcactatttg tttcagtttc aaccaatcaa 2940 tgaatagtca atatctaaac taggtaaaca tgggttattt ttcaaaatat caactaattt 3000 catacgattg tccaacgtac tttccttata aaatcctctt atcccgccca taccataaac 3060 ctgcattagt tcacaaaaac caataagagt ttcaatttta ttgatc 3106 // |
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