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EBI DbfetchID CR931644; SV 1; linear; genomic DNA; STD; PRO; 13844 BP. XX AC CR931644; XX DT 30-JUN-2005 (Rel. 84, Created) DT 18-SEP-2008 (Rel. 97, Last updated, Version 5) XX DE Streptococcus pneumoniae strain 573/62 (serotype 8). XX KW . XX OS Streptococcus pneumoniae OC Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Streptococcus. XX RN [1] RP 1-13844 RX DOI; 10.1371/journal.pgen.0020031 RX PUBMED; 16532061. RA Bentley S.D., Aanensen D.M., Mavroidi A., Saunders D., Rabbinowitsch E., RA Collins M., Donohoe K., Harris D., Murphy L., Quail M.A., Samuel G., RA Skovsted I.C., Kaltoft M.S., Barrell B., Reeves P.R., Parkhill J., RA Spratt B.G.; RT "Genetic analysis of the capsular biosynthetic locus from all 90 RT pneumococcal serotypes"; RL Plos Genet. 2(3):e31-e31(2006). XX RN [2] RP 1-13844 RA Bentley S.D.; RT ; RL Submitted (09-DEC-2004) to the EMBL/GenBank/DDBJ databases. RL Submitted on behalf of the Pathogen Sequencing Unit, Sanger Institute, RL Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: RL sdb@sanger.ac.uk XX CC NOTE: This sequence was generated from a PCR product representing the CC region from dexB to aliA and is not necessarily responsible for the CC expressed capsule serotype. For a detailed description of how CDS CC products were predicted see the associated publication. XX FH Key Location/Qualifiers FH FT source 1..13844 FT /organism="Streptococcus pneumoniae" FT /strain="573/62" FT /mol_type="genomic DNA" FT /db_xref="taxon:1313" FT CDS <1..134 FT /codon_start=3 FT /transl_table=11 FT /gene="dexB" FT /locus_tag="SPC08_0001" FT /db_xref="UniProtKB/TrEMBL:Q4JZI8" FT /protein_id="CAI32944.1" FT /translation="SNEEQDLTVEGKVKSVLIENTLAQEVFEKQILVPWDAFCVELL" FT CDS 293..458 FT /pseudo FT /transl_table=11 FT /gene="aliB" FT /locus_tag="SPC08_0002" FT /product="putative oligopeptide-binding protein AliB FT (pseudogene)" FT CDS complement(459..1334) FT /pseudo FT /transl_table=11 FT /gene="tnp" FT /locus_tag="SPC08_0003" FT /product="putative IS630-Spn1 transposase" FT /note="member of homology group 4" FT misc_feature complement(945..1316) FT /note="HMMPfam hit to PF01710, Transposase, score 3.1e-23" FT CDS 1568..3013 FT /transl_table=11 FT /gene="wzg" FT /locus_tag="SPC08_0004" FT /product="integral membrane regulatory protein Wzg" FT /note="member of homology group 0" FT /db_xref="GOA:Q4K2P0" FT /db_xref="InterPro:IPR004474" FT /db_xref="UniProtKB/TrEMBL:Q4K2P0" FT /protein_id="CAI32947.1" FT /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAFR FT YLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNRLN FT ATSNYSEYSLSVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLTVDQ FT SSSYLVAYKSLIAGETKAIVLNSVFENIIELEYPDYASKIKKIYTKGFTKKVEAPKTSK FT NQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTSRDAYVPIADGGNNQK FT DKLTHAGIYGVDSSIHTLENLYGVDINYYVLLNFTSFLKMIDLLGGVDVHNDQEFSALH FT GKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTSTEVLKNYS FT TIINSLQDSIQTNMPIETMIDLVNTQLESGGNYKVNFQDLKGTGRMDLPSYAMPDSNLY FT MMEIDDSSLAVVKAAIQDVMEGR" FT misc_feature 1568..1684 FT /note="Signal peptide predicted for SPC0263 by SignalP 2.0 FT HMM (Signal peptide probability 0.999) with cleavage site FT probability 0.540 between residues 39 and 40" FT misc_feature 1772..2119 FT /note="HMMPfam hit to PF02916, DNA polymerase processivity FT factor, score 3.1e-62" FT misc_feature 2297..2740 FT /note="HMMPfam hit to PF03816, Cell envelope-related FT transcriptional a, score 2.3e-67" FT CDS 3015..3746 FT /transl_table=11 FT /gene="wzh" FT /locus_tag="SPC08_0005" FT /product="protein-tyrosine phosphatase Wzh" FT /EC_number="3.1.3.48" FT /note="member of homology group 1" FT /db_xref="GOA:Q9AHA6" FT /db_xref="InterPro:IPR016667" FT /db_xref="UniProtKB/TrEMBL:Q9AHA6" FT /protein_id="CAI32948.1" FT /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRRK FT GMFETPEEKIAESFLQVREIAKEVADDLVIAYGAEIYYTPDVLDKLGKKRIPTLNDSRY FT ALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALGNNEKRVRELIDMGCYTQVN FT SSHVLKPKLFGERYKFMKKRAQYFLEQNLVHVIASDMHNLDGRPPHMAEAYDFVTQKYG FT EAKAQELFIDNPRKIIMDQLI" FT misc_feature 3018..3626 FT /note="HMMPfam hit to PF02811, PHP domain, score 2.7e-36" FT CDS 3752..4447 FT /transl_table=11 FT /gene="wzd" FT /locus_tag="SPC08_0006" FT /product="capsular polysaccharide biosynthesis protein Wzd" FT /note="member of homology group 2" FT /db_xref="GOA:Q9AHA5" FT /db_xref="InterPro:IPR005829" FT /db_xref="UniProtKB/TrEMBL:Q9AHA5" FT /protein_id="CAI32949.1" FT /translation="MMKEQNTIEIDVFQLFKTLWKRKLMILLVALVTGAGAFAYSTFIV FT KPEYTSTTRIYVVNRNQGDKSGLTNQDLQAGSYLVKDYREIILSQDALEKVATNLKLDM FT PAKTLASKVQVTVPTDTRIVSISVKDKQPEEASRIANSLREVAVERIVAVTRVSDVTTL FT EEARPATTPSSPNVRRNSLFGFLGGAVVTVIAVLLIELFDTRVKRPEDVEDVLQIPLLG FT LVPDLDKMK" FT misc_feature 3752..3868 FT /note="Signal peptide predicted for SPC0265 by SignalP 2.0 FT HMM (Signal peptide probability 0.721) with cleavage site FT probability 0.467 between residues 39 and 40" FT misc_feature 3773..4189 FT /note="HMMPfam hit to PF02706, Chain length determinant FT protein, score 1e-55" FT misc_feature 4295..4372 FT /note="PS00217 Sugar transport proteins signature 2." FT CDS 4457..5146 FT /transl_table=11 FT /gene="wze" FT /locus_tag="SPC08_0007" FT /product="tyrosine-protein kinase Wze" FT /EC_number="2.7.1.112" FT /note="member of homology group 3" FT /db_xref="GOA:Q9AHA4" FT /db_xref="InterPro:IPR005702" FT /db_xref="UniProtKB/TrEMBL:Q9AHA4" FT /protein_id="CAI32950.1" FT /translation="MPTLEISQAKLDFVKKAEEYYNSLCMNLQLSGDGLKVFSITSVKL FT GEGKSTTSTNIAWAFARAGYKTLLIDGDIRNSVMLGVFKARDKITGLTEFLSGTTDLSQ FT GLCDTNIENLFVIQAGSVSPNPTALLQSKNFSTMLETLRKYFDYIIVDTAPVGVVIDAA FT IITRKCDASILVTEAGEINRRDIQKAKEQLEHTGKPFLGVVLNKFDTSVNKYGSYGNYG FT DYGKNKK" FT CDS 5161..6528 FT /transl_table=11 FT /gene="wchA" FT /locus_tag="SPC08_0008" FT /product="undecaprenylphosphate glucosephosphotransferase FT WchA (initial sugar transferase)" FT /note="member of homology group 5" FT /db_xref="GOA:Q4K2N6" FT /db_xref="InterPro:IPR003362" FT /db_xref="UniProtKB/TrEMBL:Q4K2N6" FT /protein_id="CAI32951.1" FT /translation="MNGKVVNSSLAIIHSFLVILLTYLLSAVREAEIASATAIALYILH FT YFVFYISDYGQDFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYFLT FT LHALLVYVLNLFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDRLIESNEVVGELVAV FT SVLDKPDFQHDCLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMGIDVT FT VNLNAFDRSLARNKQIREMAGLNVVIFSTTFYKTSHVIAKRIIDIVGALVGLILCGLVS FT VVLVPLIRKDGGSAIFAQTRIGKNGRHFTFYKFRSMCVDAEDKKRELMEQNTMQGGMFK FT VDDDPRITKVGRFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPEQKRRLSF FT KPGITGLWQVSGRSEIKNFDEVVKLDVAYIDDWTIWKDIEILLKTVKVVLMKDGAK" FT misc_feature 5161..5268 FT /note="Signal peptide predicted for SPC0267 by SignalP 2.0 FT HMM (Signal peptide probability 0.810) with cleavage site FT probability 0.396 between residues 36 and 37" FT misc_feature 5944..6525 FT /note="HMMPfam hit to PF02397, Bacterial sugar transferase, FT score 5.3e-157" FT CDS 6533..7012 FT /transl_table=11 FT /gene="wciQ" FT /locus_tag="SPC08_0009" FT /product="putative glycosyl transferase (in conjunction FT with wciR) WciQ" FT /note="member of homology group 220" FT /db_xref="GOA:Q4K2N5" FT /db_xref="InterPro:IPR013969" FT /db_xref="UniProtKB/TrEMBL:Q4K2N5" FT /protein_id="CAI32952.1" FT /translation="MKNKKKVCFCASSGGHYEQLMKLEPIMRQQDSYLITEYTDYNKGE FT SEKKDINKVFYLLQINRLEFFWIVKLIWISLKSLYILLRIRPKVVICTGVLAMIPFCLL FT SKMFGAKLIYIESFAKVTTPTLTGRILYRYADRFYVQWESMLDIYPNAVYKGGLY" FT CDS 7013..7492 FT /transl_table=11 FT /gene="wciR" FT /locus_tag="SPC08_0010" FT /product="putative glycosyl transferase (in conjunction FT with wciQ) WciR" FT /note="member of homology group 221" FT /db_xref="GOA:Q9AHA2" FT /db_xref="InterPro:IPR007235" FT /db_xref="UniProtKB/TrEMBL:Q9AHA2" FT /protein_id="CAI32953.1" FT /translation="MIFITVGTQKHPFNRLLKKIDQLIELEVIKEEVFAQIGASDYKPK FT YFSYNSFLSSIDMNEKMREASMVITHGGTASIVKALKLRKKIIAVPRLEQFGEHVDNHQ FT LQIITVLGEEGYILPCFDIEQLGTVYQKAQTFTTKPYISNSYALLEDIRQYIDSL" FT misc_feature 7013..7489 FT /note="HMMPfam hit to PF04101, Glycosyltransferase family, FT score 4.8e-59" FT CDS 7505..8569 FT /transl_table=11 FT /gene="wciS" FT /locus_tag="SPC08_0011" FT /product="putative glycosyl transferase WciS" FT /note="member of homology group 222" FT /db_xref="GOA:Q9X9A5" FT /db_xref="InterPro:IPR001296" FT /db_xref="UniProtKB/TrEMBL:Q9X9A5" FT /protein_id="CAI32954.1" FT /translation="MTKVLQIGPSINLSKGGMSAVIRDLTYSPLLSGDFDITAYQSFID FT GSLVTRLTYSSYALLKFVVHSGNYDIYHIHTATRGSCWRKLLYLKLLKSKNKKAILHIH FT GAEFQIFYDSLPEYKKNKVREMLELSDYVIVLSQTWYDFFSNININAKIVIVENGVDTS FT FYVEKKKSITSNNFLFLGRMGKRKGAYDLIDAMNQAVAINPNLHLTMAGDGELEDIRQK FT ISNLNLTDHITIYDWVNQRDKKILFQANQTLILPSYNEGLPMAILEAMASGLAIISTPV FT GGIPEIIHEDNGWLIQPGDISQLSNIILEASYNPDVVSLMGSNNHKLVEEKYSFHSMHG FT KIKKIYNTLLETKK" FT misc_feature 7988..8488 FT /note="HMMPfam hit to PF00534, Glycosyl transferases group, FT score 1.3e-33" FT CDS 8569..10029 FT /transl_table=11 FT /gene="wzx" FT /locus_tag="SPC08_0012" FT /product="flippase Wzx" FT /note="member of homology group 47" FT /db_xref="GOA:Q9X9A6" FT /db_xref="InterPro:IPR002797" FT /db_xref="UniProtKB/TrEMBL:Q9X9A6" FT /protein_id="CAI32955.1" FT /translation="MTKKSITRNFIFNTILTMSSFIFPLISFPYVSRVLMADGLGRVTF FT ASSIIDYALTFAMLGMPLYGVKVCAGYIKNKEKLSQTVRELLVINLLVGFLVMLLLATM FT VIIIPRFRQEWQLIIITSSTIPLNIIGIEWLYKALEDYSYISIRTLTFKILGFLLMFLV FT VRTKDDYMVYAAITVLASHGSFILNFFRSRTFLLENISQRLNLKQHIKPLLILFFLSVS FT WTIYKNTDVVMMGFLTSDTEIGYYSTALKIRSIVLSVVTSLGTVVLPRLVKYYKEGKYN FT EAKKILNKSSSFIMLSSLYFIGYIVINAREIILFIAGRNYLGAIPTLQVSIFSAIFVGY FT SIMYGTNILVSIGKENVTIQASIIGVVLNICLNFIMIPKFAALGAGIATSIGEAVMVLY FT EIIYLGKDGWSYFERLNILKIIVVFIFSTFMLYIMKDFFVGYPLFIYIVISGVIYSIIY FT IFGLMILRENLLSSWKISILNRFNIIKE" FT misc_feature 8569..8679 FT /note="Signal peptide predicted for SPC0271 by SignalP 2.0 FT HMM (Signal peptide probability 0.910) with cleavage site FT probability 0.543 between residues 37 and 38" FT misc_feature 8584..9393 FT /note="HMMPfam hit to PF01943, Polysaccharide biosynthesis FT protein, score 1.6e-40" FT CDS 10042..10770 FT /transl_table=11 FT /gene="wciT" FT /locus_tag="SPC08_0013" FT /product="putative glycosyl transferase" FT /note="member of homology group 211" FT /db_xref="GOA:Q9AHA0" FT /db_xref="InterPro:IPR007577" FT /db_xref="UniProtKB/TrEMBL:Q9AHA0" FT /protein_id="CAI32956.1" FT /translation="MIPKKIHYIWFGGSEKPDVVLKCINSWKKYMPDYEIVEWNEDNFD FT LSDSQFAKSAYESRKWAFASDYARFKILSKYGGIYFDTDVELLKTIPDDILAHSSFTGF FT EYIGEVNPGLVYACMPDDKIAKYMVQYYEQASFDINHLVTVNTIITDYLLKNNFQKNNQ FT FQIIDGLAIYPDDYFCGYDQEVKEVRLTERTISIHHYSATWKTRTLKRKVQMIVKTIIG FT AENYRKLLYCKRKITNAVSK" FT misc_feature 10114..10380 FT /note="HMMPfam hit to PF04488, Glycosyltransferase FT sugar-binding regi, score 8.3e-27" FT CDS 10754..11917 FT /transl_table=11 FT /gene="wzy" FT /locus_tag="SPC08_0014" FT /product="oligosaccharide repeat unit polymerase Wzy" FT /note="member of homology group 223" FT /db_xref="UniProtKB/TrEMBL:Q9X9A8" FT /protein_id="CAI32957.1" FT /translation="MPYLNNVPQNKFSIRLFDIAIVLLTLKMLVSSIPVFDFVFPQKFQ FT NILVILGYILIFLHIFEKRKYTLQFIISIILITTLLLYTSIQMQNYVYFTSWFMLIGTI FT HYDLRRVIKIIFIVSLSIMFISIFISLLMYIIDYKREILINIRRNETVRAFTFGFIHPN FT KFTIVLSNLCLMFIWLIKDRLKYYHVTFCLFIQLFFYFFTQTRTALLVSIVIFALLYIY FT MFVENLELRWIGYSFFCISTFLGVLAFQFYPSNNKFSIFIDNILTGRIKLAAYARTFFG FT YTFWGQYVDKEIVWDPIWGLTSFTFDSFYSFLMSNAGIIWLLILSVLFVKLQKYLDNKS FT LILLLAWSMYAVTETDLIFPSYGFQFLFLSILFTNTSTCSTIMLKNN" FT CDS 11986..13224 FT /transl_table=11 FT /gene="ugd" FT /locus_tag="SPC08_0015" FT /product="UDP-glucose 6-dehydrogenase Ugd" FT /note="member of homology group 20" FT /db_xref="GOA:Q9AH99" FT /db_xref="HSSP:1DLJ" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/TrEMBL:Q9AH99" FT /protein_id="CAI32958.1" FT /translation="MKIAVTGTGYVGLSIAVLLAQHHQVIAVDIIPEKVELINQRKSPI FT QDDYIEKYLAEKDLNLVATLDAKEAYKDADFVVIAAPTNYDPKKNFFDTSAVESVIETV FT LKYNPDAIMVIKSTVPVGYTESARKKFQTENILFSPEFLRESKALYDNLYPSRIILGTD FT KNNERLVSVAETFAGLLQEGALKEDIDTLYMGFTEAEAVKLFANTYLALRVSYFNELDT FT YSEMKGLDTQAIIDGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYEDVPEELIGA FT IVRSNSTRKDFIADQVLKMVDYYDYSAHNVFNERVEKDIIIGIYRLTMKSNSDNFRQSS FT IQGIMKRIKAKGVRVVIYEPTLESGSTFFGSSIINDLEEFKRLSNAIVANRYDNSLEDV FT KEKVYTRDIFERD" FT misc_feature 11986..12066 FT /note="Signal peptide predicted for SPC0274 by SignalP 2.0 FT HMM (Signal peptide probability 0.610) with cleavage site FT probability 0.508 between residues 27 and 28" FT misc_feature 11986..12402 FT /note="HMMPfam hit to PF00056, lactate/malate FT dehydrogenase, NAD bind, score 0.00018" FT misc_feature 11986..12498 FT /note="HMMPfam hit to PF01210, NAD-dependent FT glycerol-3-phosphate deh, score 3.4e-05" FT misc_feature 11986..12528 FT /note="HMMPfam hit to PF03721, UDP-glucose/GDP-mannose FT dehydrogenase, score 6.7e-53" FT misc_feature 12571..12852 FT /note="HMMPfam hit to PF00984, UDP-glucose/GDP-mannose FT dehydrogenase, score 4.3e-38" FT misc_feature 12952..13203 FT /note="HMMPfam hit to PF03720, UDP-glucose/GDP-mannose FT dehydrogenase, score 4.9e-06" FT CDS 13286..13381 FT /pseudo FT /transl_table=11 FT /gene="HG265" FT /locus_tag="SPC08_0016" FT /product="UDP-galactopyranose mutase (pseudogene)" FT /note="member of homology group 265" FT CDS 13435..13635 FT /pseudo FT /transl_table=11 FT /gene="HG266" FT /locus_tag="SPC08_0017" FT /product="putative acetyltransferase (fragment)" FT /note="member of homology group 266" FT CDS 13797..>13844 FT /transl_table=11 FT /gene="aliA" FT /locus_tag="SPC08_0018" FT /db_xref="UniProtKB/TrEMBL:Q4JZ89" FT /protein_id="CAI32961.1" FT /translation="MMKSSKLLALAGVTLL" XX SQ Sequence 13844 BP; 4482 A; 2028 C; 2704 G; 4630 T; 0 other; tgtccaatga agagcaagac ttgacagtag aaggaaaagt caaatctgtc ttgattgaaa 60 acaccctagc tcaagaagtt tttgaaaaac aaatcttagt tccatgggat gctttctgtg 120 tggaattact ataaatattt tttgcagaaa aatttaaaat tgaaatcata taaaaacaag 180 ggaggactgt ataaaaagca gaaatccttt gttttttata accaaggttt ataaactttc 240 attttcgaaa ttcaattaac tttacaaatt cccactatta aggagaaaga agatgaacat 300 aaagaagcgt gtccttagtg caggcctgac ttttgcatct gctttgcttt tagctgcttg 360 cggccaatca ggctcagata caaaaactta ctcatcaacc tttagtggaa atccaactac 420 atttaactat ctattagact attacgctga taatatagtc aattgaaaca agagcaggac 480 aaaagagcct cgtaaaaggt attgcaactt ggtaatacct ttttgaggtg ctttttgata 540 tgagcccatg ttttctcaat aggattgtac tcaggtgagt agggaggaag aggtaaaagt 600 ttacgcctaa actcttcaca caagagttct agcttcccca ttctatggaa tcttgcatta 660 tccataataa taaccgatgg tgtggttaat gttggtaaga gaaacttctg aaaccaagct 720 tcaaaaaagt cgctcgtcat cgtctcttcg taagtcattg gagcgattaa ctcaccattc 780 atttgttaga cctgcaacca aagaaatcct ctgatatctt cttccagata ctttgcctct 840 tattaactga ttaactgact ttttaatgag cgaccatatt ctcgataaaa ataagtatcg 900 aatcctgttt catcaatcta aacaggtgct aggtgcttta aactattaaa attcttaaga 960 aataaggcta ctttttctgg gtcttgttca tagtaggtgt ggttcttttt tcgagtgtag 1020 cccatagctt tgagcgcata gtggatggta gttggatgac agccaaattc agaagctatt 1080 tcagtcaaat aagcatctgg attgtcagta agatagtttt taagtctatc tctatcaact 1140 tttcttggtt ttgttccttt tacttggtgg tttagctctc ctgttttctc ttttagcttt 1200 aaccagccat aaatggtatt acgtgagatt tggaaaacgt gtgatgcttc tgttatacta 1260 cctgttcgct cacaataaga gagaactttt ttacgaaaat ctattgaata tgccataaga 1320 agattatacc tcattgtgta ctatagtaga ttgaaactag aatagtacac atctgcttct 1380 aaaacattgt tagaaatcga tttgactgtc ccgatcgatt tgtcatgttc ttatttcatt 1440 ttactatatt tttgttttgc gggaagtcta ctaagatact taaagatgca gatagtgaaa 1500 aaaaggtgta gacattaccg taaaaaagtg atataatcgt aagatgttca atgtataggt 1560 gttaatcatg agtagacgtt ttaaaaaatc acgttcacag aaagtgaagc gaagtgttaa 1620 tatagttttg ttgactattt atttattgtt agtttgtttt ttattgttct taatctttaa 1680 gtacaatatc cttgctttta gatatcttaa tctagtggta actgcgttag tcctactagt 1740 tgccttggta gggctactct tgattatcta taaaaaagct gaaaaattta ctatttttct 1800 gttggtgttc tctatccttg tcagctcagt gtcgctcttt gcagtacagc agtttgttgg 1860 actgaccaat cgtttaaatg cgacttctaa ttactcagaa tattcgctca gtgtcgctgt 1920 tttagcagat agtgagatcg aaaatgttac gcaactgacg agtgtgacag caccgactgg 1980 gactgataat gaaaatattc aaaaactact agctgatatc aagtcaagtc agaataccga 2040 tttgacggtc gaccagagtt cgtcttactt ggttgcttac aagagtttga ttgcagggga 2100 gactaaggcc attgtcctaa atagtgtctt tgaaaatatc atcgagttag agtatccaga 2160 ctacgcatcg aagataaaga agatttatac caagggattc actaaaaaag tagaagctcc 2220 taagacgtct aagaatcagt ctttcaatat ctatgttagt ggaattgaca cctatggacc 2280 tattagttcg gtgtcgcgat cagatgtcaa tatcctgatg actgtcaatc gtgataccaa 2340 gaaaatcctc ttgaccacaa cgtcacgtga tgcctatgta ccaatcgcag atggtggaaa 2400 taatcaaaaa gataaattaa cccatgcggg catttatgga gttgattcgt ccattcacac 2460 cttagaaaac ctctatggag tggatatcaa ttactatgtg ctattgaact tcacttcttt 2520 cttgaaaatg attgacttat tgggaggggt agatgttcat aatgatcaag agttttcagc 2580 tctacatggg aagttccatt tcccagtagg gaatgtccat ctagactctg agcaggctct 2640 aggttttgta cgtgaacgct actcactagc cgatggagac cgtgaccgtg gtcgcaacca 2700 acaaaaggtg attgtggcta tccttcaaaa attaacgtca accgaagtac tgaaaaatta 2760 tagtacgatc attaatagct tgcaagattc tatccaaaca aatatgccga ttgagactat 2820 gatagattta gtgaatactc agttggaaag tggaggtaat tataaagtaa attttcaaga 2880 tttaaaaggg acaggtcgga tggatcttcc ttcttatgca atgccagaca gtaacctcta 2940 tatgatggaa atagatgata gtagtttagc tgtagttaaa gcagctatac aggatgtgat 3000 ggagggtaga tgaaatgata gacatccatt cgcatatcgt ttttgatgta gatgacggtc 3060 ccaagtcaag agaggaaagc aaggctctct tggcagaatc ctacaggcag ggggtgcgaa 3120 ccattgtctc tacctctcac cgtcgcaagg gtatgtttga aactccggaa gagaagatag 3180 cagaaagctt tcttcaggtt cgggaaattg caaaagaagt agcagatgat ttagtcattg 3240 cttacggggc tgaaatttac tacacaccag atgttctgga taagctggga aaaaagcgga 3300 ttccgaccct caatgatagt cgttatgcct tgatagagtt tagtatgaac actccttatc 3360 gcgatattca tagcgccttg agcaagatct tgatgttggg aattactcca gtcattgccc 3420 acattgagcg ctatgatgct cttggaaata atgaaaaacg cgttcgagaa ctgattgata 3480 tgggctgtta cacgcaagta aatagttcac atgtcctcaa accaaaactt tttggagaac 3540 gttataaatt catgaaaaaa agagctcagt attttttaga gcagaatttg gttcatgtca 3600 ttgcaagtga catgcacaat ctagacggca gacctcctca tatggcagaa gcatatgact 3660 ttgttaccca aaaatacgga gaagcgaagg ctcaggaact ttttatagac aatcctcgaa 3720 aaattataat ggatcaacta atttaggaga aatgatgaaa gaacaaaaca cgatagaaat 3780 cgatgtattt caattattta aaaccttgtg gaaacgcaag ctaatgattt tattagtggc 3840 acttgtgaca ggtgcggggg cttttgcata tagcactttt attgttaagc cagaatatac 3900 gagtaccacg cgaatttacg tagtgaatcg caatcaagga gacaagtcgg ggctgacaaa 3960 tcaggatttg caggcaggat cttatctggt aaaagactac cgtgagatta tcctttcgca 4020 ggatgcattg gaaaaagtag cgacaaattt gaagttggac atgccagcaa aaacgttagc 4080 tagcaaagtt caagtgactg taccaactga cactcgtatc gtctcaatct ctgtcaagga 4140 taaacagcca gaggaagcca gtcgtatcgc taattctcta cgagaagttg ctgtagaaag 4200 gatcgtcgct gtaacgcgag tatctgatgt aacgacactt gaagaagcgc gaccagctac 4260 gactccttct tctccaaatg ttcgacgcaa ttccttgttt ggttttcttg gaggagcagt 4320 tgtaacagta attgctgttc ttttgattga gttgttcgac acccgtgtga aacgtcctga 4380 agatgttgaa gatgtactgc aaattccact tttagggctc gttccagatt tggacaaaat 4440 gaaataggag gaagttatgc caacattaga aatctcacag gcaaaattgg attttgtaaa 4500 aaaggcagag gaatattata actctttgtg catgaaccta cagttaagtg gagatggttt 4560 gaaagtattt tctatcactt ctgtgaaact aggagaagga aaatcaacga cttccaccaa 4620 tatcgcttgg gcttttgcgc gtgcaggtta caaaacgctg ctgattgatg gagatattcg 4680 caattctgtt atgttaggtg tctttaaagc aagggataag attacaggcc tgacagaatt 4740 tttatcagga actacagacc tatcacaagg gctttgtgat accaatatcg aaaatctctt 4800 tgtaattcag gctggctctg tgtcaccgaa tccgacagct cttcttcaaa gtaagaattt 4860 cagtacaatg cttgaaacct tgcgtaaata ttttgactac atcattgtag atactgctcc 4920 tgtcggtgtc gtgattgatg cggctattat tacgcgaaaa tgcgatgctt ctattttagt 4980 gacggaggca ggtgaaataa atcgacggga tattcaaaaa gcaaaagaac agttggaaca 5040 cacagggaag ccgtttttgg gagttgtgtt gaataaattc gatacttcag taaacaaata 5100 cggttcttat ggaaattatg gagattacgg gaaaaataaa aaatagattg agggatagag 5160 atgaatggaa aagtagtaaa ttcttcattg gccataatcc atagttttct tgttatttta 5220 ttgacttatc tacttagtgc tgtgagagaa gcggagattg cttcagcaac agctattgca 5280 ctttatatcc tccattattt tgtcttttat atcagtgatt atggacagga tttctttaaa 5340 aggggatatt tgattgaact tgtccagaca ttgaaatata tcctattctt tgcactagcg 5400 attagtattt ctaatttttt cttagaggat cgatttagta tttccagacg aggcatgatt 5460 tacttcctca cattacatgc tctcttagtc tatgtgctaa acctatttat caagtggtat 5520 tggaagcggg cttatcccaa ctttaaagga agtaagaaga ttctcctact tacagcaact 5580 tctcgtgtcg aaaaggtact ggatagatta atagaatcaa atgaggttgt tggggagttg 5640 gtagccgtca gtgtcttaga taaaccagat tttcagcatg attgtttaaa ggtagtagca 5700 gagggggaga tagtaaactt tgcgactcat gaggtggtcg atgaagtctt tatcaatctt 5760 ccaagtgaaa aatacaatat tggagagctt gtctctcagt ttgaaacgat gggaattgat 5820 gtaacagtca atctaaatgc ttttgatcgt agtttggcac gtaacaagca aattcgtgag 5880 atggcaggat taaacgttgt gattttttct acaacatttt ataagactag ccacgtgatt 5940 gctaagcgga ttattgatat cgtgggtgca ttggtagggc tgatactatg tggtctagtc 6000 agtgttgtac tggtcccttt gattcgaaag gatgggggct ctgctatttt tgctcagacg 6060 cgtattggaa aaaatggtcg ccacttcact ttttacaagt tccgctccat gtgtgtagat 6120 gctgaggaca aaaaaagaga actcatggaa caaaatacca tgcagggtgg aatgtttaag 6180 gtggatgatg acccacgtat cacgaaagtt ggtcgtttta tacggaagac tagcttggac 6240 gagctgccac agttttataa tgttctaaag ggagatatga gtttggttgg tacacgacca 6300 ccaacagtgg acgagtatga acactatact ccagaacaaa aacgtcgtct aagttttaaa 6360 cctggtataa caggtttatg gcaggttagt ggacgaagtg agattaagaa tttcgatgaa 6420 gttgtcaagt tagatgtagc ttatatagat gattggacaa tctggaaaga tattgaaatc 6480 ttattgaaga cagttaaagt tgtattgatg aaggatggag cgaagtaaag gaatgaaaaa 6540 taaaaaaaaa gtatgttttt gtgcttcgtc agggggacat tatgagcaac taatgaagct 6600 tgaaccgatt atgagacaac aagacagcta tttaatcaca gaatatactg attataacaa 6660 aggagagtct gagaagaaag atataaacaa ggtgttttat cttttgcaaa ttaatcgttt 6720 ggaatttttt tggatcgtta aattgatttg gatttccttg aaatcacttt atatactgtt 6780 aagaatacgc ccaaaagtag ttatctgtac aggtgttctt gcaatgatac cgttttgttt 6840 gctttcaaaa atgtttgggg ctaaattaat ttatattgaa tcttttgcaa aagtgaccac 6900 tcctacttta acaggtagaa tactataccg ttacgcagat agattctatg tgcaatggga 6960 gagtatgcta gatatctacc caaatgctgt ttataaaggg gggctttatt aggtgatttt 7020 tataactgta gggactcaaa aacacccttt taatcgcttg ttaaaaaaaa tagatcaact 7080 tattgaatta gaagtgataa aggaagaggt gtttgctcag attggagctt ctgattataa 7140 acctaaatac ttttcttata attcattttt gagttcaatt gatatgaatg agaagatgag 7200 agaagctagt atggtaatta cacatggtgg gactgctagt attgttaaag ctttgaaatt 7260 aagaaaaaaa attatagcgg ttccacgatt agagcagttt ggagaacatg tggataatca 7320 ccaattacaa atcatcacag ttcttggtga ggaaggttat attttgccat gctttgatat 7380 agagcagtta ggaactgttt atcaaaaagc tcagactttt acaacaaagc catatatttc 7440 taatagttat gcacttttag aagatatacg acaatacata gatagtcttt gaaaggaaat 7500 ttagatgaca aaagttttgc aaattgggcc tagtataaat ttatctaaag gtggtatgag 7560 cgctgttatt agagatttga catatagccc gttattgtca ggtgattttg atataactgc 7620 ttatcaatct tttattgatg gctctcttgt aacacggtta acctattcta gttatgctct 7680 tcttaaattt gtagttcatt ctggtaatta tgacatatac catattcaca ctgctacaag 7740 agggagttgt tggagaaaat tactgtattt gaaattactt aaaagtaaaa ataaaaaagc 7800 tattttgcat attcatggag cagaatttca aatattttat gactcacttc ctgagtataa 7860 aaaaaataaa gttagagaaa tgttagaatt atctgattat gtgattgtac tttcccaaac 7920 ttggtatgat tttttttcaa atattaatat aaatgcaaaa attgtaatag ttgaaaatgg 7980 tgtagacact tctttttatg tagaaaaaaa gaaaagtata acaagcaaca actttctttt 8040 tttaggaagg atgggcaaaa gaaaaggagc ctatgattta atagatgcca tgaatcaagc 8100 agtggctata aatcctaatt tgcatttgac aatggctgga gatggagaac ttgaagacat 8160 acgtcaaaag atatcaaatc tgaatttgac tgatcatatt acaatatatg attgggttaa 8220 tcagagagat aaaaaaatac tatttcaagc taatcagact ttgatattgc cttcttataa 8280 tgaggggctc ccgatggcaa tattggaagc tatggcttct ggattagcaa tcatatcaac 8340 gcctgtaggt ggcatacctg aaattataca cgaggataat ggctggttaa ttcaaccggg 8400 tgatatttct cagttatcta atattatttt agaagcaagt tacaatcccg atgttgtaag 8460 tttaatgggg agcaataatc ataagttggt tgaagagaag tatagttttc atagtatgca 8520 tggtaaaatt aaaaaaattt ataatactct attagaaacg aaaaaataat gactaaaaaa 8580 tctattacta gaaattttat tttcaataca attctaacta tgtccagttt tatttttcca 8640 ctcatcagtt tcccatatgt ttcaagggtt ctgatggcag atgggttggg acgagtaacg 8700 ttcgcttctt caattattga ttatgcccta acatttgcaa tgttagggat gccattatat 8760 ggtgttaaag tctgtgcagg atatattaaa aataaggaga agctaagtca gactgtcaga 8820 gaattgttag tcattaattt attagtagga tttcttgtga tgcttctttt agcaactatg 8880 gtaataataa ttcctcgttt tagacaggaa tggcagctta ttattatcac gtcatctaca 8940 attcctctca atattatagg aatagagtgg ttatacaaag cattggaaga ctattcgtat 9000 atttcaatta gaacattgac ttttaaaata ttaggtttcc ttttgatgtt tttagtcgtc 9060 cgaacaaaag atgactatat ggtatatgcg gcaattacag ttttagctag tcatggttct 9120 tttattttga atttttttag atctagaact ttcctactag aaaatatctc tcaaagattg 9180 aatttaaagc aacatataaa gccgttatta attttatttt ttttatctgt atcatggact 9240 atttataaga atacagatgt cgttatgatg ggatttctaa ctagtgatac agaaattggt 9300 tactattcaa cagcattaaa aattcgttct atagtgctca gtgtagtaac ttctttaggg 9360 acagttgtac tacctcgttt agtcaaatat tataaagaag gaaagtataa tgaagcaaag 9420 aaaatactaa acaaaagttc ttcatttatt atgctaagtt ctttatattt tattggctat 9480 attgtaatta atgccagaga aattattctt tttattgcgg gtaggaatta cttaggggct 9540 atcccgacac ttcaagtatc tatttttagt gcaatatttg ttggctactc tattatgtat 9600 ggaaccaata tcctagtatc tataggtaaa gaaaatgtaa ccattcaggc aagtattatt 9660 ggtgttgttt taaatatttg tttgaatttt attatgatac ccaaatttgc tgcactaggg 9720 gcaggtattg caacttctat aggagaagct gttatggtat tatatgaaat aatatacttg 9780 ggaaaggatg gttggagcta ttttgaaaga ttaaatattt taaagattat tgtagtattt 9840 atatttagta cgtttatgtt atatattatg aaagattttt ttgttggcta tcctttattt 9900 atttatattg taatttcagg ggttatttat agtattatat atatatttgg tcttatgata 9960 ctaagagaaa atttgctttc aagttggaaa atatcaatac taaatcggtt taatattatt 10020 aaagaatgag gagaatatta tatgattcct aaaaaaatcc actatatttg gttcggtgga 10080 agtgagaaac cagatgttgt cttaaaatgt attaattctt ggaaaaaata tatgccagac 10140 tatgagattg ttgaatggaa tgaggataat tttgatttat cagattcaca atttgcgaag 10200 tctgcatatg aatctaggaa atgggcattt gcatcggatt atgctcgttt taaaatttta 10260 tctaagtatg gtgggatcta ttttgatacg gatgttgagt tgttaaaaac aataccagat 10320 gatatattag ctcattcatc ttttacaggt tttgaatata ttggtgaagt aaatccagga 10380 cttgtatatg cttgtatgcc agatgataag atagcaaaat atatggtgca atattatgag 10440 caagcaagtt ttgatatcaa tcatttggta actgtcaata caattattac agattatcta 10500 ttaaaaaata actttcaaaa aaataatcag ttccaaataa ttgatggatt agcaatttat 10560 ccagatgatt acttttgtgg ttatgatcag gaggtcaagg aggttaggct tacggaacgg 10620 acgattagta ttcatcatta tagtgctact tggaaaacaa gaactttaaa aagaaaagta 10680 cagatgattg ttaaaacaat tataggagct gagaattata ggaaattatt atattgtaag 10740 aggaaaataa ccaatgccgt atctaaataa tgtaccgcaa aataaatttt ctataagatt 10800 atttgatatt gctatagttt tattgacttt aaagatgtta gtttcttcga ttccagtttt 10860 tgactttgtg tttcctcaaa aatttcaaaa tatccttgtt attttaggtt atatattgat 10920 ttttttacat atttttgaga aaagaaagta cacattacaa tttataatta gtataattct 10980 aattactaca ttactgcttt atactagtat acaaatgcaa aattatgttt actttacgag 11040 ttggtttatg ttgattggta cgattcatta tgatttaaga agagtaataa aaattatatt 11100 tattgtatca ttaagtatta tgttcatatc aatttttata tcacttctta tgtatataat 11160 tgattataaa cgagaaattc ttattaatat ccgaagaaac gaaactgtca gagcatttac 11220 atttggtttt atacatccca ataaatttac tattgtacta tctaacttat gtcttatgtt 11280 tatttggtta atcaaagatc gtttgaaata ttatcatgta actttttgtc tttttattca 11340 gttattcttt tattttttca cgcagactag aacagctcta ctagtatcta tagtaatttt 11400 tgctctttta tatatttata tgtttgttga aaatcttgaa cttagatgga taggatactc 11460 attcttttgt atttctactt ttttaggagt tttggcattt caattttatc cttctaataa 11520 taagttttca atttttattg ataatatcct gactggacgt attaaattag ctgcatacgc 11580 aagaaccttt tttggttata cattctgggg tcaatatgtt gataaagaaa ttgtttggga 11640 tcctatttgg ggattaacta gtttcacttt tgattcgttt tattccttct tgatgagtaa 11700 tgctggcatc atatggctac ttatcttatc agtcttattt gtaaaactac aaaaatattt 11760 agacaataaa agtttgattt tattattagc ttggtctatg tatgcggtaa cagaaactga 11820 tttaatattt ccaagttatg gattccagtt cttattttta agtatacttt ttactaatac 11880 tagtacatgt tctacaataa tgttaaagaa taattaattg aaggaatcta tatattttct 11940 aatttgtaga atgataggca aatatatgag gagggaagaa aaagtatgaa aatagcagta 12000 acaggtacgg gatatgttgg cctatccatt gcggttttat tggctcagca tcatcaagta 12060 atagcggtgg acattattcc tgagaaggtc gagttaatta atcaaagaaa gtcacctatt 12120 caagatgact acatagagaa atatctggca gaaaaggatt taaatttagt ggctacattg 12180 gatgcaaaag aagcatataa agatgcggat tttgttgtga ttgcagctcc aactaactat 12240 gatccaaaga agaacttctt tgacacttct gctgtagagt ctgtcattga aacggtgctt 12300 aaatataatc cagatgctat catggtcatt aaaagtacgg ttccagttgg ttatacagag 12360 tcagctcgaa agaaatttca gactgaaaat atcttattta gtcctgaatt tttgcgtgag 12420 tcaaaagcac tttacgataa cttatatccc agccgtatta ttttagggac agataaaaat 12480 aatgaacgct tggtaagtgt tgctgagacg tttgcgggat tgctccaaga aggcgcttta 12540 aaagaagata ttgatacatt gtacatgggc tttactgaag ctgaggcggt caagcttttt 12600 gcaaatactt acctagcttt gcgtgtttct tattttaatg agctggatac ttattctgag 12660 atgaaggggc tagatactca agctattatt gatggagttg gtttggatcc acgaatcggc 12720 tctcactata acaatccttc cttcgggtat ggtggttact gtctccctaa agataccaag 12780 caacttcttg ccaactatga agatgtacca gaagaactta taggagctat agtacggagc 12840 aattcgacac gtaaagactt tattgccgat caggttctta agatggtaga ttattatgat 12900 tattcagccc ataatgtttt taatgagaga gtagaaaaag atattattat tggtatctat 12960 agattgacca tgaagagtaa tagcgataat tttcgtcaaa gttctattca aggtattatg 13020 aaacgtataa aagctaaggg agtaagagta gttatttatg aaccaacttt agaaagtggg 13080 agtacatttt ttggaagttc cattatcaat gatttagagg aattcaaacg actgagtaat 13140 gccattgttg ctaatcgcta tgataactca ttagaagatg tcaaggaaaa agtctataca 13200 cgtgatattt ttgaaagaga ttgatttagt ataataaaaa tgtaaaaaat taattgttag 13260 aagcggtcta tactgagaag caatgggacg cttagcagat tatacatatt acgatatgca 13320 tgttgtgatt gaacgagcgc tcgaagttat agaaaaagag tttgaaaatg acaaaaatta 13380 gaatcaattg gatagatttt ggaaaagggt tctcaatatt tttagtttta gtagagcgtc 13440 tatatcctcc attccccaat ttgtagtatg attctaattc tgatgttgaa agtgggaatt 13500 agctctactt tacttcatat tgttatcgga attgttttgg gctggcattt atccatccta 13560 gcaacctata tattgaaaaa aattccattt ttgaatattg ttttattacc acagaagtat 13620 attaaattaa aataaattat tttggctctt tgtcagctaa catctggaga ggacaatcac 13680 cgtcttctcc tttttggtct tttcagaata tactaaacta acccaaaaat tctgaaaatt 13740 ctgttgacat ctttttgaaa agagtctata atggagagaa agttttaaag gagaaaatga 13800 tgaaaagttc aaaactactt gcccttgcgg gcgtgacatt attg 13844 // |
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