EBI Dbfetch

ID   CR761607; SV 2; linear; mRNA; STD; VRT; 1903 BP.
XX
AC   CR761607;
XX
DT   09-SEP-2004 (Rel. 81, Created)
DT   04-OCT-2006 (Rel. 89, Last updated, Version 6)
XX
DE   Xenopus tropicalis finished cDNA, clone TGas067g11
XX
KW   .
XX
OS   Xenopus (Silurana) tropicalis (western clawed frog)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Mesobatrachia; Pipoidea; Pipidae; Xenopodinae; Xenopus;
OC   Silurana.
XX
RN   [1]
RP   1-1903
RA   Amaya E., Ashurst J.L., Bonfield J.K., Croning M.D.R., Chen C-K.,
RA   Davies R.M., Francis M.D., Garrett N., Gilchrist M.J., Grafham D.V.,
RA   McLaren S.R., Papalopulu N., Rogers J., Smith J.C., Taylor R.G., Voigt J.,
RA   Zorn A.M.;
RT   ;
RL   Submitted (04-OCT-2006) to the EMBL/GenBank/DDBJ databases.
RL   Sanger Institute, Hinxton, Cambridgeshire, CB10 1SA, UK. E-mail enquiries:
RL   trop@sanger.ac.uk
XX
CC   Sanger Xenopus tropicalis EST/cDNA project.
CC   
CC   This sequence is from a Xenopus Gene Collection (XGC) library, from a
CC   library constructed by Aaron M. Zorn. cDNA was prepared from RNA extracted
CC   from gastrula embryos. EcoRI-NotI cut cDNA was then ligated into pCS107
CC   with EcoRI at the 5' end and NotI at the 3' end.
CC   Vector: pCS107; Site_1: EcoRI; Site_2: NotI
CC   Host: Escherichia coli XL1-blue
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1903
FT                   /organism="Xenopus (Silurana) tropicalis"
FT                   /mol_type="mRNA"
FT                   /dev_stage="gastrula (stage 10.5-13 mixed)"
FT                   /clone_lib="XGC-gastrula"
FT                   /clone="TGas067g11"
FT                   /db_xref="taxon:8364"
FT   mRNA            1..1881
FT                   /gene="pi4k2b"
FT                   /locus_tag="TGas067g11.1-001"
FT                   /product="phosphatidylinositol 4 kinase type II beta"
FT   CDS             5..1483
FT                   /gene="pi4k2b"
FT                   /locus_tag="TGas067g11.1-001"
FT                   /standard_name="OTTXETP00000001534"
FT                   /product="phosphatidylinositol 4 kinase type II beta"
FT                   /note="match: proteins: Q8TCG2"
FT                   /db_xref="GOA:Q28G26"
FT                   /db_xref="InterPro:IPR018936"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q28G26"
FT                   /protein_id="CAJ83481.1"
FT                   /translation="MEPKQTADARDSPPLLVFLEPAAEEVTAHTAPLSPNPQSARAAPG
FT                   SAVRFFSDSAREEEAGEDEPLLKKSGPVSPRAARKGRTRLSSSSDRENMSGGHVGNGEF
FT                   NVILDDLEFADIIHRAEQAIESGVFPERISQGSSGSYFVKDPKGKIIGVFKPKSEEPYG
FT                   HLNPKWTKYFHKICCPCCFGRGCLVPNQGYLSEAGAYLVDEKLGLGVVPKTKVVWLVSE
FT                   TFNYSAIDRAKSRGKKYALEKVPKVGRKFHRIGLPPKVGSFQLFVDGYKEADYWLRKFE
FT                   TDPLPENTRKQLQCQFEKLVILDYVIRNTDRGNDNWLIRYDSQDDDELMEKGDDFPLKD
FT                   WKEIKEPVIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLPQAKVPFSQETRDLILPRISD
FT                   MNFVQDLCEDLYELFKTDKGFDKATFEKQMSVMRGQILNLTQALKDGKTPIQLVQMPRV
FT                   VVERSCSGSQGRIVQMSNAFTQTFHCRKPFFSSW"
FT   polyA_signal    1850..1855
FT   polyA_site      1881
XX
SQ   Sequence 1903 BP; 562 A; 364 C; 460 G; 517 T; 0 other;
     ggacatggag ccgaagcaaa cagccgacgc tagggactcc ccgccgttgc tggtgttcct        60
     ggagcccgct gctgaagaag tgacggctca caccgctccg ctctctccca acccccagtc       120
     agccagagct gctcctggca gcgccgtgcg cttcttttcc gatagcgccc gggaggaaga       180
     ggccggggag gatgagccgc tgctgaagaa gtctggcccg gtgtcccctc gggctgcgag       240
     gaaagggagg actcggctta gttcgtcctc ggacagagag aacatgagcg ggggtcacgt       300
     tggaaatggg gagtttaatg tcattttgga tgacttggaa tttgctgata taattcaccg       360
     agcagagcaa gccatagaga gcggtgtgtt tccagaaaga atctctcaag gatccagtgg       420
     aagttacttt gttaaagatc caaaagggaa aattattggc gtgtttaaac caaaatcaga       480
     ggagccttat gggcatctaa atccaaaatg gaccaaatat tttcacaaga tttgctgccc       540
     ctgctgcttt ggtcgtggat gtcttgttcc taatcagggg tacctttctg aggctggtgc       600
     ctatctggta gatgagaaat tgggattagg tgttgttcca aaaaccaagg tagtgtggct       660
     tgtaagcgaa acattcaatt acagtgcaat agatcgagca aaatcgcgag gcaaaaaata       720
     tgctttagaa aaggtgccca aagtgggtag aaagtttcac cgtatcggac tacctcctaa       780
     ggttggttcc ttccagctct ttgttgatgg ctataaggaa gctgactact ggctaaggaa       840
     atttgaaaca gaccccttac ctgaaaatac cagaaagcaa cttcagtgtc agtttgagaa       900
     attagtaata cttgattatg ttattagaaa tacggaccga ggcaatgata attggctaat       960
     taggtatgac agtcaggatg acgatgagtt gatggaaaag ggagacgatt ttcctctaaa      1020
     ggattggaag gaaatcaagg aaccagtaat caaaattgct gcaattgaca atggtttagc      1080
     ttttcctttt aaacatcccg atgaatggag agcgtacccg ttccactggg cttggcttcc      1140
     ccaagctaaa gttcctttca gtcaggaaac cagagattta attctgccac gcatctctga      1200
     tatgaatttc gtccaggacc tttgtgaaga tctttatgaa ctttttaaga ctgataaagg      1260
     atttgacaag gctacatttg aaaaacagat gtctgtgatg agaggacaga ttctgaatct      1320
     gacgcaggca ttaaaagatg gcaaaactcc tattcagcta gtacagatgc ctcgtgtggt      1380
     ggtggagcgt tcctgcagcg gcagccaagg cagaattgtg cagatgagca atgcatttac      1440
     acagactttt cattgcagaa aacccttctt ctcttcctgg taacaggaga aatgttatta      1500
     taaagctgct cttttgcaca agcactgcaa taaagtgaaa cctgctgtcg gtcgtcaact      1560
     gccaaaaccc tcgggctgtg gtactaatta gtattctgaa tgtaattaaa aggtttacag      1620
     ttcagtccgt attgtaacgt cagggattca cataggactg tgttggctgt actgtatttt      1680
     tatatgacta attaaacttg tacattttgg aggaaaaaaa atgttcaaat ggttgtgctc      1740
     cttcaggggg ttcctatatg ctggaatgat aatgccgtaa atatagcaat gcaaagtaga      1800
     tttatttata atgtaaaaag ctgatatttt gtaatattat atttgccata ttaaatgatg      1860
     aaataaaatg ttttttctca ctttaaaaaa aaaaaaaaaa aaa                        1903
//