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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   CR761081; SV 2; linear; mRNA; STD; VRT; 1293 BP.
XX
AC   CR761081;
XX
DT   09-SEP-2004 (Rel. 81, Created)
DT   04-OCT-2006 (Rel. 89, Last updated, Version 6)
XX
DE   Xenopus tropicalis finished cDNA, clone TEgg049g04
XX
KW   .
XX
OS   Xenopus (Silurana) tropicalis (western clawed frog)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Mesobatrachia; Pipoidea; Pipidae; Xenopodinae; Xenopus;
OC   Silurana.
XX
RN   [1]
RP   1-1293
RA   Amaya E., Ashurst J.L., Bonfield J.K., Croning M.D.R., Chen C-K.,
RA   Davies R.M., Francis M.D., Garrett N., Gilchrist M.J., Grafham D.V.,
RA   McLaren S.R., Papalopulu N., Rogers J., Smith J.C., Taylor R.G., Voigt J.,
RA   Zorn A.M.;
RT   ;
RL   Submitted (04-OCT-2006) to the EMBL/GenBank/DDBJ databases.
RL   Sanger Institute, Hinxton, Cambridgeshire, CB10 1SA, UK. E-mail enquiries:
RL   trop@sanger.ac.uk
XX
CC   Sanger Xenopus tropicalis EST/cDNA project.
CC   
CC   This sequence is from a Xenopus Gene Collection (XGC) library, from a
CC   library constructed by Aaron M. Zorn. cDNA was prepared from RNA extracted
CC   from eggs. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at
CC   the 5' end and NotI at the 3' end.
CC   Vector: pCS107; Site_1: EcoRI; Site_2: NotI
CC   Host: Escherichia coli XL1-blue
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FH   Key             Location/Qualifiers
FH
FT   source          1..1293
FT                   /organism="Xenopus (Silurana) tropicalis"
FT                   /mol_type="mRNA"
FT                   /dev_stage="egg"
FT                   /clone_lib="XGC-egg"
FT                   /clone="TEgg049g04"
FT                   /db_xref="taxon:8364"
FT   mRNA            1..1275
FT                   /gene="mdh1"
FT                   /locus_tag="TEgg049g04.1-001"
FT                   /product="malate dehydrogenase 1, NAD (soluble)"
FT   CDS             73..1077
FT                   /gene="mdh1"
FT                   /locus_tag="TEgg049g04.1-001"
FT                   /standard_name="OTTXETP00000005314"
FT                   /product="malate dehydrogenase 1, NAD (soluble)"
FT                   /note="match: proteins: P40925 Q80Y13"
FT                   /db_xref="GOA:Q6DIY9"
FT                   /db_xref="InterPro:IPR016040"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q6DIY9"
FT                   /protein_id="CAJ81507.1"
FT                   /translation="MPEPVKVLVTGAAGQIAYSLLYGIAKGDVFGKDQPLILVLLDITP
FT                   MMTVLEGVVMELQDCALPLLKEVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKA
FT                   NVKIFKSQGAALNKYSKKSVKVIVVGNPANTNCLTAMKSAPSIPKENFSCLTRLDHNRA
FT                   KGQIALKLNVASDDVKNVIIWGNHSSTQYPDASHATVNLQGKDVGAFEAVKNDDWLKGE
FT                   FITTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPDGQFVSMGVISDGNSYGIP
FT                   EDLMYSFPLTIKNKTWKIVEGLPINDFSREKMDLTAKELHEEKETALEFLSCE"
FT   polyA_signal    1244..1249
XX
SQ   Sequence 1293 BP; 383 A; 250 C; 301 G; 359 T; 0 other;
     agcagctagc tcagaagcag cgactcgcag cgcacggaaa ggccattgcc atctgcacgt        60
     ttcagaaaca aaatgcctga acctgtgaaa gtccttgtga ccggagctgc tggtcagatt       120
     gcttactccc tgctctatgg tattgctaaa ggcgatgtct ttggaaaaga ccagcctctt       180
     atacttgttc ttttggatat tacccctatg atgactgtac ttgaaggtgt agtgatggag       240
     ttgcaggact gtgctctgcc cctgctgaaa gaggtgattg ctaccgataa ggaagatgtg       300
     gccttcaaag atttggatgt tgccattctg gttggttcca tgccaagaag ggagggcatg       360
     gaaagaaagg acttgctgaa agccaatgta aagattttta agtctcaggg tgcagctctg       420
     aacaagtatt ccaagaagag tgttaaggtt attgttgtgg ggaatccagc aaacacaaat       480
     tgcctgactg caatgaagtc tgctccatcc atccctaaag agaacttcag ctgtctgaca       540
     cgcttagatc acaacagagc caaaggacaa attgctctga agttgaatgt tgcttctgat       600
     gatgtaaaga atgtaattat ctggggtaat cactcatcca ctcagtatcc tgatgcttcc       660
     catgcaactg tgaatctgca aggaaaagat gttggtgcct ttgaagcagt gaagaatgat       720
     gactggctaa agggtgaatt tatcactaca gtgcagcaaa gaggagcagc tgttattaag       780
     gccagaaagt tgtctagtgc aatgtctgct gctaaagcca tctgcgatca tgtgagggat       840
     atctggtttg gaacccctga tgggcagttt gtttccatgg gagttatttc agatggaaac       900
     tcttatggta tacctgaaga cctgatgtat tcattccctc ttaccatcaa gaataagacc       960
     tggaaaatag tggaaggcct gcctattaat gacttttccc gtgagaaaat ggatctcaca      1020
     gctaaagaac ttcatgaaga aaaagaaact gcacttgaat ttctgtcctg cgaatgacta      1080
     gtgacagatt ccacaatgca cttaacccct gagaaaatga aaagttctct ttaagtttcc      1140
     taccaagtgc tatttatgtt gtctagcagt ggtcacctga cagtcgcact tgattttgtt      1200
     ttgtatatgt gactagtgtt atcaatcatt cctagcaata gtcattaaat gaaaaccctg      1260
     ggtggtttta aagtgaaaaa aaaaaaaaaa aaa                                   1293
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