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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   BU415425; SV 1; linear; mRNA; EST; VRT; 770 BP.
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AC   BU415425;
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DT   03-DEC-2002 (Rel. 74, Created)
DT   28-FEB-2006 (Rel. 87, Last updated, Version 4)
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DE   603667886F1 CSEQRBL06 Gallus gallus cDNA clone ChEST607k22 5', mRNA
DE   sequence.
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KW   EST.
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OS   Gallus gallus (chicken)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archosauria; Dinosauria; Saurischia; Theropoda; Coelurosauria; Aves;
OC   Neognathae; Galliformes; Phasianidae; Phasianinae; Gallus.
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RN   [1]
RP   1-770
RX   DOI; 10.1016/S0960-9822(02)01296-4
RX   PUBMED; 12445392.
RA   Boardman P.E., Sanz-Ezquerro J., Overton I.M., Burt D.W., Bosch E.,
RA   Fong W.T., Tickle C., Brown W.R.A., Wilson S.A., Hubbard S.J.;
RT   "A comprehensive collection of chicken cDNAs";
RL   Curr. Biol. 12(22):1965-1969(2002).
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DR   Ensembl-Gn; ENSGALG00000013406; Gallus_gallus.
DR   Ensembl-Tr; ENSGALT00000007629; Gallus_gallus.
DR   GOA; Q5NDL3.
DR   InterPro; IPR007657; Glycosyltransferase_AER61.
DR   UNILIB; 27673; 11233.
DR   UniProtKB/Swiss-Prot; Q5NDL3; AER61_CHICK.
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CC   Contact: Simon Hubbard
CC   Department of Biomolecular Sciences
CC   University of Manchester Institute of Science and Technology (UMIST
CC   )
CC   PO Box 88, Manchester, M60 1QD, UK
CC   Tel: 01612008930
CC   Fax: 01612360409
CC   Email: Simon.Hubbard@umist.ac.uk.
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FH   Key             Location/Qualifiers
FH
FT   source          1..770
FT                   /organism="Gallus gallus"
FT                   /lab_host="DH10B"
FT                   /strain="Layer and broiler"
FT                   /mol_type="mRNA"
FT                   /sex="Male and female"
FT                   /dev_stage="adult"
FT                   /clone_lib="CSEQRBL06"
FT                   /clone="ChEST607k22"
FT                   /tissue_type="Abdominal fat pad"
FT                   /note="Vector: pBluescript II KS(+); Site_1: EcoRI; Site_2:
FT                   Not1; Modification of pBluescript II KS(+) [Stratagene]
FT                   vector to accommodate cDNA produced with the T-trimmed
FT                   protocol (Construction of uni-directionally cloned cDNA
FT                   libraries from messenger RNA for improved 3' end DNA
FT                   sequencing by Glenn Fu, et al. U.S. Patent # 6,387 ,624).
FT                   Cut pBluescript II KS(+) with NotI and EcoRI. Ligate in
FT                   double stranded adaptor containing BsgI and BamHI sites
FT                   [5'ggccgcgtgcagccccggatccgaaaaaaag]
FT                   [5'aattctttttttcggatccggggctgcacgc]"
FT                   /db_xref="taxon:9031"
FT                   /db_xref="UNILIB:27673"
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SQ   Sequence 770 BP; 172 A; 232 C; 173 G; 193 T; 0 other;
     ctgtcgcggg atgtgcagcc gcgctccgct cctcctccgc tccgctaggt ccgtgcgccc        60
     gcggcgtctg cggtgcttct ccccaaagcc gacagaagct gtaagctgcc atgttcatct       120
     tgctgatgtt tgtattgcta ctgcaagaaa tcctggctaa ttccagagat gaaaatctca       180
     ctgagctcaa tagcgttctg gaagagccca cgtacagcta cagggctatc aacctaccag       240
     ccgagcatat tccgtacttc ctgcacaaca atcggcacat tgctggcatc tgcaagcagg       300
     actctcgctg cccgtataaa aaatacttga agaaactaaa atcctgcatt tcaaagtact       360
     ctttggacat cacttaattc attttgacag cagccccgtg caagaaatgt ggccgtctcc       420
     attgtctgaa tgggataact gagtccatga ccggcccgag gcagacgggg aggagagctg       480
     agtgcagggg gcatgggggc aggggagcag tgagctcctg tctccaacag ctcagttaca       540
     aaagcacctg tggccgtcag cctgttctaa ccacacatat tgagggtgtt gatcgcatgt       600
     aatccccacc tgctgtcctg gccaggctct tttcaacaaa ttattcccca gagagcaaga       660
     cctgaccttt ttcttctgat ttggatctcc cgtcccttaa atccttccct ctgctgccag       720
     tacctccagt tctctcttcc ctccctccct ctccttcgaa ctacttctca                  770
//
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