EBI Dbfetch

ID   BT026161; SV 1; linear; mRNA; STD; MAM; 2226 BP.
XX
AC   BT026161;
XX
DT   14-JUL-2006 (Rel. 88, Created)
DT   27-NOV-2008 (Rel. 97, Last updated, Version 2)
XX
DE   Bos taurus methionine-tRNA synthetase 2 (mitochondrial) (MARS2), nuclear
DE   gene encoding mitochondrial protein, mRNA, incomplete 5' cds.
XX
KW   FLI_CDNA.
XX
OS   Bos taurus (cattle)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
XX
RN   [1]
RP   1-2226
RX   DOI; 10.1101/gr.170101
RX   PUBMED; 11282978.
RA   Smith T.P., Grosse W.M., Freking B.A., Roberts A.J., Stone R.T., Casas E.,
RA   Wray J.E., White J., Cho J., Fahrenkrug S.C., Bennett G.L., Heaton M.P.,
RA   Laegreid W.W., Rohrer G.A., Chitko-McKown C.G., Pertea G., Holt I.,
RA   Karamycheva S., Liang F., Quackenbush J., Keele J.W.;
RT   "Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries
RT   and construction of a gene index for cattle";
RL   Genome Res. 11(4):626-630(2001).
XX
RN   [2]
RP   1-2226
RX   DOI; 10.1186/1471-2164-6-166
RX   PUBMED; 16305752.
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences";
RL   BMC Genomics 6:166-166(2005).
XX
RN   [3]
RP   1-2226
RA   Harhay G.P., Sonstegard T.S., Van Tassell C.P., Clawson M.L., Heaton M.P.,
RA   Keele J.W., Snelling W.M., Weidmann R.T., Smith T.P.L.;
RT   "Sequencing and analysis of Bos taurus full-length insert cDNA clones";
RL   Unpublished.
XX
RN   [4]
RP   1-2226
RA   Harhay G.P., Sonstegard T.S., Van Tassell C.P., Clawson M.L., Heaton M.P.,
RA   Keele J.W., Snelling W.M., Weidmann R.T., Smith T.P.L.;
RT   ;
RL   Submitted (13-JUL-2006) to the EMBL/GenBank/DDBJ databases.
RL   Molecular Genetic Research Unit, USDA-ARS U.S. Meat Animal Research Center,
RL   P.O. Box 166, Spur 18D, Clay Center, NE 68933
XX
DR   Ensembl-Gn; ENSBTAG00000002056; Bos_taurus.
DR   Ensembl-Tr; ENSBTAT00000002662; Bos_taurus.
XX
CC   Contact: Gregory P. Harhay at harhay@email.marc.usda.gov.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2226
FT                   /organism="Bos taurus"
FT                   /lab_host="Escherichia coli DH10B"
FT                   /mol_type="mRNA"
FT                   /clone_lib="1BOV"
FT                   /clone="1BOV28J18; plate 28 row J column 18"
FT                   /tissue_type="pooled"
FT                   /note="Vector: pCMV SPORT6; Site_1: NotI; Site_2: SalI;
FT                   Library made from pooled tissue from lymph node, ovary,
FT                   fat, hypothalamus, and pituitary."
FT                   /db_xref="taxon:9913"
FT   gene            1..2226
FT                   /gene="MARS2"
FT                   /note="putative, similar to GeneID: 92935"
FT   CDS             <1..1772
FT                   /codon_start=3
FT                   /gene="MARS2"
FT                   /product="methionine-tRNA synthetase 2 precursor"
FT                   /db_xref="GOA:A6H7E1"
FT                   /db_xref="InterPro:IPR002304"
FT                   /db_xref="InterPro:IPR009080"
FT                   /db_xref="InterPro:IPR014758"
FT                   /db_xref="InterPro:IPR015413"
FT                   /db_xref="UniProtKB/Swiss-Prot:A6H7E1"
FT                   /protein_id="ABG67000.1"
FT                   /translation="SAFRLLGRRGASRVSLLEDFSFRYYSSGPLGVRDDTRDSRAYFTT
FT                   PIFYVNAAPHIGHLYSALLADALCRHHRLRVPSDAATGFSTGTDEHGLKIQQAAAAAGL
FT                   APSELCDRVSAQFQQLFREADISSTDFIRTTEARHRIAVQHFWGMLKSRGLLYKGLYEG
FT                   WYCASDECFLPEAKVTRQPGPSGDLCPVSLESGHPVSWTKEENYIFRLSQFREPLQQWL
FT                   RGDPQAITPEPFHHTVLQWLEEELPDLSVSRRSSHLHWGIPVPGDDSQTIYVWLDALVN
FT                   YLTVVGYPDAEFKSWWPNTSHIIGKDILKFHAIYWPALLLGAGMSPPHRIYVHSHWTVC
FT                   GQKMSKSLGNVVDPRTCLDRYTVDGFRYFLLRQGVPSWDCDYYDEKVVKLLDSELADAL
FT                   GGLLNRCTANKINPSGIYPAFCATCFPSEPGLVGPSGRAQAEDYALVSAVATLPKQVAD
FT                   HYDNFQIYKALEAVSSCVRQTNGFVQRHAPWKLNWESPVDAPWLGTVLHVALECLRVFG
FT                   TLLQPVTPSLADRLLSRLGVSSTERSLGELHFLSRFYGHPSPFEGRRLGPETGVLFPRL
FT                   DQSRSWLVKAHKT"
XX
SQ   Sequence 2226 BP; 441 A; 612 C; 589 G; 584 T; 0 other;
     tttctgcctt ccggctgcta ggtcgcagag gggcgagtag ggtctcgctc ttggaggact        60
     ttagcttccg ctactacagc tcgggtcctc ttggtgtccg cgacgatacc cgcgactcgc       120
     gcgcctactt taccacacct attttctacg tgaacgccgc gccgcacatc gggcacctgt       180
     actccgcgct gctggcggac gccctgtgcc gacaccatcg cctccgagtt cccagtgacg       240
     ccgccacggg attctccacg ggtaccgatg agcacggcct gaagattcag caggcagccg       300
     ccgccgcggg cctggctccg tccgagctgt gcgacagagt ctctgcccag ttccagcagc       360
     ttttccggga ggctgacatc tcttccaccg acttcatccg caccaccgag gcccggcacc       420
     ggatagctgt gcagcatttc tgggggatgc tgaagtctcg gggtcttctc tataagggcc       480
     tctatgaagg ttggtattgc gcctccgacg agtgcttcct gcctgaagcc aaggtcacca       540
     ggcagcccgg cccgtcaggg gatttgtgtc ctgtatctct cgagagcgga cacccggtct       600
     cctggaccaa ggaagaaaac tacattttta ggctttccca gttccgggag ccgctccagc       660
     agtggctgcg gggcgaccct caggccataa caccggagcc attccatcac acagtccttc       720
     agtggttgga ggaggagctg ccggacctat cggtctctcg aaggagtagc cacttgcact       780
     ggggcattcc ggtgcccggg gacgattcac agaccatcta cgtatggctg gatgccttgg       840
     tcaactacct tactgtagtt ggctacccag acgctgagtt caaatcttgg tggccgaaca       900
     cctctcatat cataggcaag gacattctca aattccatgc tatctattgg cctgccctcc       960
     tcttaggggc cggcatgagc ccaccacacc gcatctatgt ccactcccac tggacagtct      1020
     gtggtcagaa gatgtctaag agcttgggta acgtggtgga tcccaggact tgtcttgacc      1080
     gctatacggt ggatggcttc cgctactttc ttcttcggca gggcgtcccc agctgggatt      1140
     gtgattacta tgatgaaaag gtggttaagt tgttggattc cgagctggca gatgctttgg      1200
     gaggtctcct gaaccgatgt actgccaaca agatcaatcc ttctgggatc tatccggctt      1260
     tctgcgctac ttgctttccc agtgagccag gcttggtagg gccgtcaggt cgtgctcagg      1320
     cagaggacta cgctctagtg agcgcagtgg ccactttgcc caagcaggta gcagaccact      1380
     acgataactt tcagatctat aaggctctgg aggcagtgtc cagctgtgtc cggcagacta      1440
     acggctttgt ccaaaggcat gcgccatgga agctgaactg ggagagcccc gtggatgctc      1500
     cctggctggg tactgtgctt catgtggcct tggagtgttt gcgagtcttt ggaactctgc      1560
     tccagcctgt caccccaagc ctagctgaca ggctgctgtc taggttgggg gtttcttcca      1620
     cagagaggag ccttggagag ctccattttt tgtctcgatt ctatggacat ccatcccctt      1680
     ttgaagggag gagactggga cctgaaactg gggtcttgtt tccaagactg gaccagtcta      1740
     ggtcctggct ggtaaaagcc cacaagacct agaaactcag ttcttattag cttgtggtaa      1800
     aaaagtaaat ttgttatttt cttatttttt gcattttcag ggaaattata ttaatgcttt      1860
     ctaaagtgtt ggatcaaatg agcacataag tactgtccct gtgaaaagag gtttgatagc      1920
     ctttcaggag cctactccct actcttcagt tctctgtgtg cattaaccac tgtcctttgt      1980
     accccagtgt ctctgagatg tctccagggg taagagacaa ctttgctgat actattcctt      2040
     ctcattgtgt acctccgtcc aaaccagtta tttgcccaga ctacctctca tggcttgttt      2100
     tttattggcc tggcctctgc tgggcagaga gtgggggttg gaggccccct acctcagctt      2160
     ttactttgtt acatatgttg agtctcctgt tgaaaacaga ttgtttcatt aaagctttaa      2220
     aaaccc                                                                 2226
//