EBI Dbfetch

ID   BT021843; SV 1; linear; mRNA; STD; MAM; 1425 BP.
XX
AC   BT021843;
XX
DT   26-MAR-2005 (Rel. 83, Created)
DT   27-NOV-2008 (Rel. 97, Last updated, Version 3)
XX
DE   Bos taurus protein O-fucosyltransferase 1 (POFUT1), mRNA, complete cds.
XX
KW   FLI_CDNA.
XX
OS   Bos taurus (cattle)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
XX
RN   [1]
RP   1-1425
RX   DOI; 10.1101/gr.170101
RX   PUBMED; 11282978.
RA   Smith T.P., Grosse W.M., Freking B.A., Roberts A.J., Stone R.T., Casas E.,
RA   Wray J.E., White J., Cho J., Fahrenkrug S.C., Bennett G.L., Heaton M.P.,
RA   Laegreid W.W., Rohrer G.A., Chitko-McKown C.G., Pertea G., Holt I.,
RA   Karamycheva S., Liang F., Quackenbush J., Keele J.W.;
RT   "Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries
RT   and construction of a gene index for cattle";
RL   Genome Res. 11(4):626-630(2001).
XX
RN   [2]
RP   1-1425
RX   DOI; 10.1186/1471-2164-6-166
RX   PUBMED; 16305752.
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences";
RL   BMC Genomics 6:166-166(2005).
XX
RN   [3]
RP   1-1425
RA   Harhay G.P., Sonstegard T.S., Van Tassell C.P., Clawson M.L., Heaton M.P.,
RA   Keele J.W., Snelling W.M., Weidmann R.T., Smith T.P.L.;
RT   ;
RL   Submitted (17-MAR-2005) to the EMBL/GenBank/DDBJ databases.
RL   Molecular Genetic Research Unit, USDA-ARS U.S. Meat Animal Research Center,
RL   P.O. Box 166, Spur 18D, Clay Center, NE 68933, USA
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DR   Ensembl-Gn; ENSBTAG00000016231; Bos_taurus.
DR   Ensembl-Tr; ENSBTAT00000021595; Bos_taurus.
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CC   Contact: Gregory P. Harhay at harhay@email.marc.usda.gov.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1425
FT                   /organism="Bos taurus"
FT                   /lab_host="Escherichia coli DH10B"
FT                   /mol_type="mRNA"
FT                   /clone_lib="1BOV"
FT                   /clone="1BOV27L4; plate 27 row L column 4"
FT                   /tissue_type="pooled"
FT                   /note="Vector: pCMV SPORT6; Site_1: NotI; Site_2: SalI;
FT                   Library made from pooled tissue from lymph node, ovary,
FT                   fat, hypothalamus, and pituitary."
FT                   /db_xref="taxon:9913"
FT   gene            1..1425
FT                   /gene="POFUT1"
FT                   /note="putative, similar to GeneID: 23509"
FT   CDS             49..1104
FT                   /codon_start=1
FT                   /gene="POFUT1"
FT                   /product="protein O-fucosyltransferase 1 isoform 1
FT                   precursor"
FT                   /db_xref="GOA:Q7YRE6"
FT                   /db_xref="InterPro:IPR019378"
FT                   /db_xref="UniProtKB/TrEMBL:Q7YRE6"
FT                   /protein_id="AAX46690.1"
FT                   /translation="MGAAAWAPSLLPPRVSLLLLLLPLPGLPVGSWDPAGYLLYCPCMG
FT                   RFGNQADHFLGSLAFAKLLNRTLAVPPWIEYQHHKPPFTNVHVSYQKYFKLEPLQAYHR
FT                   VISLEDFMEKLAPTHWPPEKRVAYCFEVAAQRSPDKKTCPMKEGNPFGPFWDQFHVSFN
FT                   KSELFAGISFSASYKDQWIQRFSPEEHPVLALPGAPAQFPVLEEHRPLQKYMVWSDEMV
FT                   RTGEAQIHAHLIRPYVGIHLRIGSDWKNACAMLKDGTAGAHFMASPQCVGYSRHTTAPL
FT                   TMTMCLPDLKEIRRALKLWVTALNAQSVYIATDSESYLPEIQQLFKGKSLSFAVPKASE
FT                   MSLLLGDRKGK"
FT   repeat_region   1128..1208
FT                   /rpt_family="CHR-2A"
FT   repeat_region   1209..1334
FT                   /rpt_family="L1MC"
XX
SQ   Sequence 1425 BP; 308 A; 427 C; 360 G; 330 T; 0 other;
     ttccctccca gtctccgcgc cgcggcgcgc tctggcactg gacccgctat gggcgccgcc        60
     gcgtgggcac cgtcgctctt gccgccgcgt gtgtctctcc tgctgctgct tttgccgctc       120
     ccggggctgc ccgtgggctc ctgggatccg gccggttacc tgctctactg cccatgcatg       180
     gggcgttttg ggaaccaggc tgatcatttc ttgggctccc tggcatttgc aaagctgttg       240
     aaccgcaccc tggctgtacc tccttggatt gagtaccagc atcacaagcc tcctttcacc       300
     aacgtccatg tgtcctacca gaagtacttc aagctggagc ccctccaggc ctaccatcga       360
     gtcatcagcc tggaggactt catggagaag ctggcaccca cccactggcc ccctgagaag       420
     cgggtggcat actgctttga ggtggcagcc cagcgaagtc ctgataagaa gacatgcccc       480
     atgaaggaag gaaatccctt tggcccattt tgggatcagt ttcatgtgag tttcaacaag       540
     tccgagcttt ttgcaggcat ttccttcagt gcctcctaca aagaccagtg gatccagaga       600
     ttttccccag aggaacatcc agtgcttgcc ctgcctggtg cccctgccca gttccctgtc       660
     ctggaggagc acaggcccct ccagaagtac atggtgtggt cagacgagat ggtgaggaca       720
     ggggaggccc agatccacgc ccacctcatc cggccctacg tgggcattca cctgcgcatt       780
     ggctctgact ggaaaaacgc gtgtgccatg ctgaaagatg gaaccgctgg cgcccacttc       840
     atggcctccc cacaatgcgt gggctacagc cgccacacca ccgccccgct caccatgacc       900
     atgtgcctcc ctgacctgaa ggaaattagg cgcgctctga agctctgggt gacagcgctg       960
     aacgcccaat cggtctacat tgccaccgac tctgagagtt acctgcccga gatccagcag      1020
     ctcttcaaag ggaagagcct gagttttgct gttccgaagg catcagagat gtccttgctg      1080
     ctcggggaca ggaaagggaa gtgatggacc ttcatcacac tacaggcgac cttcctgatc      1140
     cagggattga acctgcatct cctgcactgg gaggcaaatg ctttaccgtt ggccacctag      1200
     gaagccccat tctgctttcc caccagcagt gaatgagagc tcccactgct tcatgttttc      1260
     acccacattt ggtgttgtcg ctcttctgga tgttggccat tctaataagt gtgtaatggt      1320
     agctacttat tattcacaat aaatatagtt ctgcttacta cataaaattt agaaaataca      1380
     aaaagtgtta agaagaaaat aagaataaac tataatgcca atatc                      1425
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