EBI Dbfetch

ID   BT021513; SV 1; linear; mRNA; STD; MAM; 1698 BP.
XX
AC   BT021513;
XX
DT   24-MAR-2005 (Rel. 83, Created)
DT   27-NOV-2008 (Rel. 97, Last updated, Version 3)
XX
DE   Bos taurus pyrroline-5-carboxylate reductase 1 (PYCR1), mRNA, complete cds.
XX
KW   FLI_CDNA.
XX
OS   Bos taurus (cattle)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
XX
RN   [1]
RP   1-1698
RX   DOI; 10.1007/s00335-001-2145-4
RX   PUBMED; 12140684.
RA   Sonstegard T.S., Capuco A.V., White J., Van Tassell C.P., Connor E.E.,
RA   Cho J., Sultana R., Shade L., Wray J.E., Wells K.D., Quackenbush J.;
RT   "Analysis of bovine mammary gland EST and functional annotation of the Bos
RT   taurus gene index";
RL   Mamm. Genome 13(7):373-379(2002).
XX
RN   [2]
RP   1-1698
RX   DOI; 10.1186/1471-2164-6-166
RX   PUBMED; 16305752.
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences";
RL   BMC Genomics 6:166-166(2005).
XX
RN   [3]
RP   1-1698
RA   Harhay G.P., Sonstegard T.S., Van Tassell C.P., Clawson M.L., Heaton M.P.,
RA   Keele J.W., Snelling W.M., Weidmann R.T., Smith T.P.L.;
RT   ;
RL   Submitted (17-MAR-2005) to the EMBL/GenBank/DDBJ databases.
RL   Molecular Genetic Research Unit, USDA-ARS U.S. Meat Animal Research Center,
RL   P.O. Box 166, Spur 18D, Clay Center, NE 68933, USA
XX
DR   Ensembl-Gn; ENSBTAG00000000042; Bos_taurus.
DR   Ensembl-Gn; ENSBTAG00000016516; Bos_taurus.
DR   Ensembl-Tr; ENSBTAT00000000046; Bos_taurus.
DR   Ensembl-Tr; ENSBTAT00000021956; Bos_taurus.
DR   Ensembl-Tr; ENSBTAT00000042618; Bos_taurus.
XX
CC   Contact: Gregory P. Harhay at harhay@email.marc.usda.gov.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1698
FT                   /organism="Bos taurus"
FT                   /lab_host="Escherichia coli DH10B"
FT                   /mol_type="mRNA"
FT                   /clone_lib="5BOV"
FT                   /clone="5BOV21E21; plate 21 row E column 21"
FT                   /tissue_type="pooled"
FT                   /note="Vector: pCMV SPORT6; Site_1: NotI; Site_2: SalI;
FT                   Library made from pooled mRNA isolated from mammary tissues
FT                   at eight physiological, developmental, and disease states."
FT                   /db_xref="taxon:9913"
FT   gene            1..1698
FT                   /gene="PYCR1"
FT                   /note="putative, similar to GeneID: 5831"
FT   CDS             90..1052
FT                   /codon_start=1
FT                   /gene="PYCR1"
FT                   /product="pyrroline-5-carboxylate reductase 1 isoform 1"
FT                   /db_xref="GOA:Q58DT4"
FT                   /db_xref="InterPro:IPR000304"
FT                   /db_xref="InterPro:IPR004455"
FT                   /db_xref="InterPro:IPR016040"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q58DT4"
FT                   /protein_id="AAX46360.1"
FT                   /translation="MSVGFIGAGQLAFALAKGFTAAGVVAAHKIMASSPDMDLATVSAL
FT                   RKMGVNLTHHNKETVQHSDVLFLAVKPHIIPFILDEIAANIEARHIVVSCAAGVTISSI
FT                   EKKLTAFQPAPKVIRCMTNTPVVVREGATVYATGTHAQVEDGRLLEQLMSSVGFCTEVE
FT                   EDLIDAVTGLSGSGPAYAFTALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLDSEQ
FT                   HPGQLKDNVCSPGGATIHALHVLESGGFRSLLIKAVEASCTRTRELQSMADQEKVSPAA
FT                   IKKTILDKVKLDSPPGTSLAPSGHSKLLPRSMAPAGKQD"
XX
SQ   Sequence 1698 BP; 341 A; 558 C; 483 G; 316 T; 0 other;
     cgtggcgggc gcggcggtgc agactggggg cgacacgtac gggagccggt gccagctgag        60
     agggcggtgg gcgcagacct ccagacacta tgagcgtggg cttcatcggg gctggccagt       120
     tggcctttgc gctggccaag ggcttcacag cagcaggtgt tgttgctgcc cacaagataa       180
     tggccagttc tccagacatg gacctggcca ccgtttctgc cctcaggaag atgggggtga       240
     acctcacgca tcacaacaag gagaccgtgc agcacagcga cgtgctcttc ctggccgtga       300
     agccacacat catccccttc atcctggatg agatcgccgc caacattgag gctcggcaca       360
     tcgtggtgtc ctgtgcagcc ggtgtcacca tcagctccat tgagaagaag ctgacggcgt       420
     tccagccagc tcccaaagtc atccgctgca tgaccaacac gccggtcgtg gtgcgggaag       480
     gggccacagt gtatgccaca ggcacccatg cccaggtgga ggacggcagg ctcctggagc       540
     agctcatgag cagcgtgggc ttctgcacgg aggtggagga ggacctgatt gatgccgtca       600
     cagggctcag tggcagtggc ccagcctatg cattcacagc cctggacgca ctggctgatg       660
     gaggtgtgaa gatgggactt ccaagacgcc tggcagtccg cctcggggcc caggctctgc       720
     tgggggctgc caagatgcta ctggactctg aacagcaccc aggccaactc aaggacaacg       780
     tctgctcacc aggcggggcc acgatccatg cgttgcacgt gctggagagt gggggctttc       840
     gctcgctgct catcaaggcc gtggaggcct cctgcacccg cacacgagag ctgcagtcca       900
     tggccgacca ggagaaggtc tcaccagctg ccatcaagaa aaccatcctg gacaaggtga       960
     aactggactc ccctcccggc acctcgctgg ccccttctgg ccactccaag ctgctgcccc      1020
     gcagcatggc cccagcaggc aaacaggact gaggggtcga gcctgcctgt gggccactct      1080
     ccaccttctc ctgggctcac agctcttgcg ctgggagtat ctagccccag gctacacagg      1140
     gtgtcctcac gctgatccac actgcccatg gcttggtcca ctcggacccg cgagtcatag      1200
     ccggggacgg ggcacatcac ctcccagtgg gaacctccat ggtcccccaa gcgtgtcctg      1260
     gtccaaaaca aggattctct ggcctcagcc tcctgattca tccgtcccca aaccacgtca      1320
     ggaccacctt cctccaaagc tcaggaggct tgagggggct ttactctccc ccctcagcat      1380
     tagctggctt cagcttgagt acaaaggtca aggccccacc cctccccacc cccaccacga      1440
     aggcccaccc aaggagagca tctgcccttt ccccactcac ctttcctcag tgtgggaagg      1500
     acctgagggt ccccgacatc ctgcagaaga gcccatggtt cttggccagc aggacatttg      1560
     agaaaaaggg tcttcagtcg gttggacttc tgaggcccca acactcacgc catctatctc      1620
     cctttcctag cctgacccac agacgctgtc agctctgtct cttaactttt taggataaat      1680
     aaattggttt ccagcccc                                                    1698
//