EBI Dbfetch

ID   BT020936; SV 1; linear; mRNA; STD; MAM; 2596 BP.
XX
AC   BT020936;
XX
DT   17-FEB-2005 (Rel. 82, Created)
DT   27-NOV-2008 (Rel. 97, Last updated, Version 5)
XX
DE   Bos taurus mannosyl (alpha-1,3-)-glycoprotein
DE   beta-1,2-N-acetylglucosaminyltransferase (MGAT1), mRNA, complete cds.
XX
KW   FLI_CDNA.
XX
OS   Bos taurus (cattle)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
XX
RN   [1]
RP   1-2596
RX   DOI; 10.1101/gr.170101
RX   PUBMED; 11282978.
RA   Smith T.P., Grosse W.M., Freking B.A., Roberts A.J., Stone R.T., Casas E.,
RA   Wray J.E., White J., Cho J., Fahrenkrug S.C., Bennett G.L., Heaton M.P.,
RA   Laegreid W.W., Rohrer G.A., Chitko-McKown C.G., Pertea G., Holt I.,
RA   Karamycheva S., Liang F., Quackenbush J., Keele J.W.;
RT   "Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries
RT   and construction of a gene index for cattle";
RL   Genome Res. 11(4):626-630(2001).
XX
RN   [2]
RP   1-2596
RX   DOI; 10.1186/1471-2164-6-166
RX   PUBMED; 16305752.
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences";
RL   BMC Genomics 6:166-166(2005).
XX
RN   [3]
RP   1-2596
RA   Harhay G.P., Sonstegard T.S., Clawson M.L., Heaton M.P., Keele J.W.,
RA   Snelling W.M., Weidmann R.T., Smith T.P.L.;
RT   ;
RL   Submitted (16-FEB-2005) to the EMBL/GenBank/DDBJ databases.
RL   Molecular Genetic Research Unit, USDA-ARS U.S. Meat Animal Research Center,
RL   P.O. Box 166, Spur 18D, Clay Center, NE 68933, USA
XX
DR   Ensembl-Gn; ENSBTAG00000001546; Bos_taurus.
DR   Ensembl-Tr; ENSBTAT00000002024; Bos_taurus.
XX
CC   Contact: Gregory P. Harhay at harhay@email.marc.usda.gov.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2596
FT                   /organism="Bos taurus"
FT                   /lab_host="Escherichia coli DH10B"
FT                   /mol_type="mRNA"
FT                   /clone_lib="3BOV"
FT                   /clone="3BOV92L1; plate 92 row L column 1"
FT                   /tissue_type="pooled"
FT                   /note="Vector: pCMV SPORT6; Site_1: NotI; Site_2: SalI;
FT                   Library made from pooled tissue from marrow, alveolar
FT                   macrophage, ovary, fetal semitendonosus muscle, and fetal
FT                   longissimus muscle."
FT                   /db_xref="taxon:9913"
FT   gene            1..2596
FT                   /gene="MGAT1"
FT                   /note="putative, similar to GeneID: 4245"
FT   CDS             201..1544
FT                   /codon_start=1
FT                   /gene="MGAT1"
FT                   /product="mannosyl (alpha-1,3-)-glycoprotein
FT                   beta-1,2-N-acetylglucosaminyltransferase"
FT                   /db_xref="GOA:Q5E9I4"
FT                   /db_xref="InterPro:IPR004139"
FT                   /db_xref="UniProtKB/TrEMBL:Q5E9I4"
FT                   /protein_id="AAX08953.1"
FT                   /translation="MLKKQSAGLVLWGAILFVAWNALLLLFFWTRPAPGRLPSDSALDD
FT                   DPASLTREVIRLAQDAEVELERQRGLLQQIREHHARWSQRWRVPTVAPPVPPRVPVTTP
FT                   PAVIPILVIACDRSTVRRCLDKLLNYRPSAEHFPIIVSQDCGHEETAQVIASYGSAVMH
FT                   IRQPDLSTIAVPPDHRKFQGYYKIARHYRWALGQVFHEFKFPAAVVVEDDLEVAPDFFE
FT                   YFQATYPLLRADPSLWCVSAWNDNGKEQMVDSSKPELLYRTDFFPGLGWLLLAELWAEL
FT                   EPKWPKAFWDDWMRRPEQRQGRACVRPEISRTMTFGRKGVSHGQFFDQHLKFIKLNQHF
FT                   VPFTQLDLSYLRQETYDRDFLARVYGAPLLQVEKVRTSERSELQEVRVQYTSRDSFKAF
FT                   AKALGVMDDLKSGVPRAGYRGIVSFLYRGRRVHLAPPQTWDGYDPSWN"
FT   repeat_region   1659..1682
FT                   /rpt_unit_seq="t"
XX
SQ   Sequence 2596 BP; 501 A; 742 C; 722 G; 631 T; 0 other;
     tgggattctc agttttggaa atggagtact ggatgggggt ttgatgatct tcagggaagc        60
     agctgagaga agaaaggtgc atggctgctt cctaatcctg gagcccagag gaagcatccc       120
     tagaacctcg ggggtgtgag ccaggcaagc tgagggcagc cttgagccct acccttcccg       180
     cctcccctgc gggggccagg atgctgaaga agcagtctgc agggcttgtg ctgtggggtg       240
     ccatcctctt tgtggcctgg aacgccctgc tgctcctctt cttttggaca cgcccagcgc       300
     ctggcaggct accctcagac agtgctcttg atgatgaccc cgccagcctc acccgcgagg       360
     tgatccgcct ggcccaggac gctgaggtgg agttggagcg gcagcggggc ctgttgcagc       420
     agatcaggga gcaccatgct aggtggagcc agcggtggag ggtacccacc gtggccccgc       480
     ccgtcccgcc acgagtgcct gtgaccactc caccagctgt aatccccatc ctggtcatcg       540
     cctgtgaccg cagcactgtt cggcgctgcc tggacaagct gctgaattac cggccttctg       600
     ctgagcactt tcccatcatt gtcagccagg actgcgggca tgaggagaca gcccaggtta       660
     tcgcctccta cggcagtgct gtcatgcaca tccggcagcc tgacctgagc accattgcag       720
     tgccacccga ccaccgaaag ttccagggct actacaagat tgctcggcac taccgctggg       780
     cactgggcca ggtctttcac gagttcaagt tcccagcagc cgtggtggta gaggatgatc       840
     tggaggtggc tccagacttc ttcgagtact ttcaggccac ttacccgctg ctgagagccg       900
     acccctccct ctggtgtgtg tctgcctgga acgacaatgg caaggagcag atggtggact       960
     ccagcaagcc cgaactgctc taccgcacag actttttccc tggcctgggc tggctgctgt      1020
     tggctgagct ttgggctgag ctggagccca agtggcccaa ggccttttgg gatgactgga      1080
     tgcgccggcc agagcagcgg cagggccggg cctgcgtgcg gcccgaaatc tccagaacaa      1140
     tgacctttgg ccgcaaaggt gtgagccacg ggcagttctt tgaccagcac ctcaagttta      1200
     tcaagctgaa ccagcacttc gtgcccttca cccagctgga cctgtcgtac ctgcgacagg      1260
     agacttatga cagggatttc cttgcacgtg tctatggtgc tcccctgctg caggtagaga      1320
     aagtgaggac cagtgagcgg agcgagctgc aggaggtgcg agtgcagtac acgagcaggg      1380
     acagcttcaa ggcctttgcc aaggccctgg gagtcatgga tgacctcaag tctggtgtcc      1440
     ccagggccgg ctaccggggc atcgtcagct tcctgtaccg gggccgccgt gtccacctgg      1500
     ccccacccca gacctgggat ggttatgatc ctagctggaa ttagcagcac ctgcgtgtcc      1560
     atcgtgggcc ccctccttgc catctcatgg cctaagatgg gaccgcagtc tctgggcttc      1620
     atcgtcctct ctgtgtttct ctcttgggtc catttatctt tttttttttt ttttttcttt      1680
     ttccgagtgg cattcaggtg cacagatgat aaggtgaggg ggtcaaatca ggggactctt      1740
     tctgggatat gttgggggag ttattgaaca agaaaccact gtgtggtagg agggacttga      1800
     gcttatcatg gccacacaca tccacattcc aggttttctc ctaggacatc ggtggagagg      1860
     ttgacaactt tagttctctt gatcaggcct cttttccctc tcctggctct tctagccaga      1920
     gtatgagccc tcctcctgta cctgtccccg accatctctg ctttctccca attggagtag      1980
     gggtaggggg ctgtattatg tgagaagggg atgtatctgt gccggaatga gactaaccaa      2040
     aggggtttca tcaccgtcag ggtccagata gagctgttag gttgtcaggg tttactgcct      2100
     cctgcctgta tttctcttct tcccctctct ttcatctctg acctcctctc agctcttctt      2160
     tctatgcagc ccagcagttc atgattctaa gatgaaatgg tgaaggggaa aagcagaatg      2220
     ttccccccag ctcatccact gtggtgggga gaaagacttg gatgctgggg cacccctccc      2280
     tcattcttcc tctaagaaac agactgcccc tctctcctct ccatccagtc ctttccaaaa      2340
     accagtcctt tccctgttgc tctaggacag ttcatgctag cctggctttt ggaggccctc      2400
     tcagtgtgag gtacagcagc caagaccatt cgacccaacc tccctccctt ctctcccaaa      2460
     catccccggg tacacagatc aggatgcaga tgggttggag agcaatgtgt gtgtttttgt      2520
     ctcttgcctg acctcagttc atggaggaaa gtttaagcta cagaattatt ttcaaaaata      2580
     aaggctgact tgtctg                                                      2596
//