EBI Dbfetch

ID   BT020691; SV 1; linear; mRNA; STD; MAM; 1913 BP.
XX
AC   BT020691;
XX
DT   17-FEB-2005 (Rel. 82, Created)
DT   27-NOV-2008 (Rel. 97, Last updated, Version 5)
XX
DE   Bos taurus 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic
DE   acid acyltransferase, delta) (AGPAT4), mRNA, complete cds.
XX
KW   FLI_CDNA.
XX
OS   Bos taurus (cattle)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
XX
RN   [1]
RP   1-1913
RX   DOI; 10.1101/gr.170101
RX   PUBMED; 11282978.
RA   Smith T.P., Grosse W.M., Freking B.A., Roberts A.J., Stone R.T., Casas E.,
RA   Wray J.E., White J., Cho J., Fahrenkrug S.C., Bennett G.L., Heaton M.P.,
RA   Laegreid W.W., Rohrer G.A., Chitko-McKown C.G., Pertea G., Holt I.,
RA   Karamycheva S., Liang F., Quackenbush J., Keele J.W.;
RT   "Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries
RT   and construction of a gene index for cattle";
RL   Genome Res. 11(4):626-630(2001).
XX
RN   [2]
RP   1-1913
RX   DOI; 10.1186/1471-2164-6-166
RX   PUBMED; 16305752.
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences";
RL   BMC Genomics 6:166-166(2005).
XX
RN   [3]
RP   1-1913
RA   Harhay G.P., Sonstegard T.S., Clawson M.L., Heaton M.P., Keele J.W.,
RA   Snelling W.M., Weidmann R.T., Smith T.P.L.;
RT   ;
RL   Submitted (16-FEB-2005) to the EMBL/GenBank/DDBJ databases.
RL   Molecular Genetic Research Unit, USDA-ARS U.S. Meat Animal Research Center,
RL   P.O. Box 166, Spur 18D, Clay Center, NE 68933, USA
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DR   Ensembl-Gn; ENSBTAG00000007668; Bos_taurus.
DR   Ensembl-Tr; ENSBTAT00000010089; Bos_taurus.
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CC   Contact: Gregory P. Harhay at harhay@email.marc.usda.gov.
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FH   Key             Location/Qualifiers
FH
FT   source          1..1913
FT                   /organism="Bos taurus"
FT                   /lab_host="Escherichia coli DH10B"
FT                   /mol_type="mRNA"
FT                   /clone_lib="1BOV"
FT                   /clone="1BOV41J5; plate 41 row J column 5"
FT                   /tissue_type="pooled"
FT                   /note="Vector: pCMV SPORT6; Site_1: NotI; Site_2: SalI;
FT                   Library made from pooled tissue from lymph node, ovary,
FT                   fat, hypothalamus, and pituitary."
FT                   /db_xref="taxon:9913"
FT   gene            1..1913
FT                   /gene="AGPAT4"
FT                   /note="putative, similar to GeneID: 56895"
FT   CDS             313..1449
FT                   /codon_start=1
FT                   /gene="AGPAT4"
FT                   /product="1-acylglycerol-3-phosphate O-acyltransferase 4
FT                   (lysophosphatidic acid acyltransferase, delta)"
FT                   /db_xref="GOA:Q5E9R2"
FT                   /db_xref="InterPro:IPR002123"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q5E9R2"
FT                   /protein_id="AAX08708.1"
FT                   /translation="MDLVALLKSHFLCHLIFCYVFIVSGLIINTIQLCTLLLWPVNKQL
FT                   FRKINCRLSYCVSSQLVMLLEWWSGTECVIYTDPRAYPKYGKENAIVVLNHKFEIDFLC
FT                   GWSLAERFGVLGGSKVLAKKELAYVPIIGWMWYFTEMVFCTRKWEQDRKTVSESLLHLR
FT                   DYPEKYFFLIHCEGTRFTEKKHQISMQVAQAKGLPSLKHHLLPRTKGFAVTVRSLRNVV
FT                   SAVYDCTLNFRNNENPTLLGVLNGKKYHADLYVRRIPLEEVPEEEDKCAAWLHKLYQEK
FT                   DAFQEEYSHTGTFPETPVVPPRRPWTLVNWLFWASMLLYPFFRFVVNMVSSGSSLTLAS
FT                   FVLVFFVASMGVRWMIGVTEIDKGSAYGNMDSKQKHSD"
FT   repeat_region   1650..1685
FT                   /rpt_unit_seq="tg"
XX
SQ   Sequence 1913 BP; 380 A; 529 C; 556 G; 448 T; 0 other;
     tgtcttacag cgcctgtaaa ctagtggcaa tttctgcacc ccagcctgct ccctttcagt        60
     gtccgatctc taagtccatg tgtccagggc tgccagaaag gagggatctg cggttcgtgc       120
     tgtttgcagg cggcttcacc tctgggcgtg gcggatgtgt gcgcccctct gtctctccct       180
     cgttatttct ctccttggct ggatttccct aaaacctctg gaaggggcga attcctgagt       240
     cccagcctcc cgccccgctg gccgtgttct gcggtgggtg ttcgctgtct gggcgaggag       300
     cacgaccccg gaatggacct cgtcgccttg ctgaagtctc acttcctctg ccacctcatc       360
     ttctgctacg tcttcattgt ctcggggctc atcatcaaca ccattcagct ctgcacgctc       420
     cttctctggc cagtgaacaa gcagctcttc cggaagatca actgccggct gtcctactgc       480
     gtctccagcc agctggtgat gctgctggag tggtggtcgg gtacggagtg tgtcatctac       540
     acggaccccc gcgcctaccc caagtacggg aaggaaaacg ccattgtcgt tctgaaccac       600
     aagtttgaaa tcgactttct ctgcggctgg agcctggctg agcgctttgg ggtcttaggg       660
     ggctccaagg tcctggctaa gaaggagctg gcctacgtcc ccatcatcgg ctggatgtgg       720
     tacttcaccg agatggtctt ctgcacgcgc aagtgggagc aggaccgcaa gactgtctcc       780
     gagagcctcc tgcacctgcg ggactacccc gagaagtact ttttcctgat tcactgcgag       840
     ggcacacggt tcacagagaa gaaacaccag atcagcatgc aggtggccca ggccaagggg       900
     ctgcccagcc tcaagcacca cctgctgcct cgcaccaagg gcttcgcggt gaccgtgagg       960
     agtttgagaa atgtagtttc agctgtatat gactgtacac tcaatttcag aaacaatgaa      1020
     aacccaacac tgctgggagt cctcaatggc aagaaatatc acgccgattt atacgttagg      1080
     cggattcctc tagaagaagt cccggaggag gaggacaagt gtgcggcctg gctgcacaaa      1140
     ctctaccagg agaaggacgc cttccaggag gagtactccc acacgggcac cttccccgag      1200
     acacccgtgg tgcccccgcg gcggccctgg acgcttgtca actggctctt ctgggcctcg      1260
     atgctcctct accccttctt tcgcttcgtc gtcaacatgg tcagcagcgg ctcctccctg      1320
     acgcttgcca gctttgttct cgtcttcttt gtggcttcca tgggggttcg atggatgata      1380
     ggcgtcacgg aaattgacaa gggctctgcc tacggcaaca tggacagcaa gcagaaacat      1440
     agcgactgac ccgggatgtg tcaccacctg aaggggacct tggggggaat ggtggacgcc      1500
     gccatcaccc tgggcgggac ccagtgatga agctagggga gcccctgctg gggaggcagg      1560
     ggctgcagtc tcagcgtcag gtggggagga ggatgtactt aaatctttcc cccagcacgc      1620
     tttagtgggc ttcgggtctc gttctttccg tgtgtgagtc tgtgtgagtg cgtgtatgtg      1680
     tgtgttaaga acatatgtga gaatgaggta tgctgagtgg gatcattgcg agggtatctg      1740
     aggtgcgggg ggagggcaga aatggggacc tcggagggtg gggggttccc tgccatcctt      1800
     tggtgctgag ttttctgtta accctggctc gctacagagt gaaagacact ttggtaagat      1860
     gactaaatta tgcctccaaa aaaacaaaca aaaattaaac tgttgggctt ctc             1913
//