EBI Dbfetch

ID   BF074326; SV 1; linear; mRNA; EST; MAM; 572 BP.
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AC   BF074326;
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DT   19-OCT-2000 (Rel. 65, Created)
DT   24-FEB-2005 (Rel. 82, Last updated, Version 4)
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DE   221711 MARC 2BOV Bos taurus cDNA 5', mRNA sequence.
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KW   EST.
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OS   Bos taurus (cattle)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
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RN   [1]
RP   1-572
RX   DOI; 10.1101/gr.170101
RX   PUBMED; 11282978.
RA   Smith T.P., Grosse W.M., Freking B.A., Roberts A.J., Stone R.T., Casas E.,
RA   Wray J.E., White J., Cho J., Fahrenkrug S.C., Bennett G.L., Heaton M.P.,
RA   Laegreid W.W., Rohrer G.A., Chitko-McKown C.G., Pertea G., Holt I.,
RA   Karamycheva S., Liang F., Quackenbush J., Keele J.W.;
RT   "Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries
RT   and construction of a gene index for cattle";
RL   Genome Res. 11(4):626-630(2001).
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DR   Ensembl-Gn; ENSBTAG00000002924; Bos_taurus.
DR   Ensembl-Tr; ENSBTAT00000003800; Bos_taurus.
DR   GOA; P0C591.
DR   HSSP; 1VHR; P51452.
DR   InterPro; IPR016130; Tyr_Pase_AS.
DR   UNILIB; 10685; 2820.
DR   UniProtKB/Swiss-Prot; P0C591; DUPD1_BOVIN.
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CC   Contact: Smith TPL
CC   USDA, ARS, US Meat Animal Research Center
CC   PO Box 166, Clay Center, NE 68933-0166, USA
CC   Tel: 402 762 4366
CC   Fax: 402 762 4390
CC   Email: smith@email.marc.usda.gov
CC   Single pass sequencing. Bases called and alt_trimmed with phred
CC   v0.980904.e. Vector identified by cross_match with the -minscore 18
CC   and -minmatch 12 options.
CC   PCR PRimers
CC   FORWARD: AGGAAACAGCTATGACCAT
CC   BACKWARD: GTTTTCCCAGTCACGACG
CC   Plate: 80  row: I  column: 23
CC   Seq primer: ATTTAGGTGACACTATAG.
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FH   Key             Location/Qualifiers
FH
FT   source          1..572
FT                   /organism="Bos taurus"
FT                   /lab_host="DH10B"
FT                   /mol_type="mRNA"
FT                   /clone_lib="MARC 2BOV"
FT                   /tissue_type="pooled"
FT                   /note="Vector: pCMV SPORT6; Site_1: XbaI; Site_2: XhoI;
FT                   Library made from pooled tissue from testis, thymus,
FT                   semitendonosus muscle, longissimus muscle, pancreas,
FT                   adrenal, and endometrium."
FT                   /db_xref="taxon:9913"
FT                   /db_xref="UNILIB:10685"
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SQ   Sequence 572 BP; 124 A; 187 C; 161 G; 99 T; 1 other;
     ggccgccaca cgccccacgg accagcgcct cctgacccgg ccaggaccca gtacgcacag        60
     ctgcagaaag gaaagaaaat cccttggctc caaaatgaca tccggagaat cgaagacagg       120
     cctcaagaac gtctacccat ctgccaagaa gctgctgccg aaggtggagg agggggaggc       180
     tgaggattac tgtactcctg gagccttcga gctggagcgt ctcttctgga aaggcagtcc       240
     ccagtacacc cacgtcaacg aggtctggcc caagctctac atcggcgatg agacaaccgc       300
     gctggaccgc tatgggctgc agaaggcagg cttcacccac gtgctgaacg ctgcccacgg       360
     ccgctggaac gtggacacgg ggcccgacta ctaccgcgac atggccatcg agtaccatgg       420
     cgtcgaggct gatgacctgc ccagctttga cctcagcgtt ntcttctacc cagctgccgc       480
     tttcattgac gctgccctcc gctatgatca caataagatc ctggttcact gcgtcatggg       540
     ccgcagccgg tcggcgactc tggtcctggc ct                                     572
//