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EBI DbfetchID BC151313; SV 1; linear; mRNA; STD; MAM; 4180 BP. XX AC BC151313; XX DT 14-AUG-2007 (Rel. 92, Created) DT 12-JUN-2008 (Rel. 96, Last updated, Version 2) XX DE Bos taurus pyruvate dehydrogenase (lipoamide) alpha 1, mRNA (cDNA clone DE MGC:165778 IMAGE:8415761), complete cds. XX KW MGC. XX OS Bos taurus (cattle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae; OC Bovinae; Bos. XX RN [1] RC NIH-MGC Project RP 1-4180 RA Moore S., Alexander L., Brownstein M., Guan L., Lobo S., Meng Y., RA Tanaguchi M., Wang Z., Yu J., Prange C., Schreiber K., Shenmen C., RA Wagner L., Bala M., Barbazuk S., Barber S., Babakaiff R., Beland J., RA Chun E., Del Rio L., Gibson S., Hanson R., Kirkpatrick R., Liu J., RA Matsuo C., Mayo M., Santos R.R., Stott J., Tsai M., Wong D., Siddiqui A., RA Holt R., Jones S.J., Marra M.A.; RT ; RL Submitted (31-JUL-2007) to the EMBL/GenBank/DDBJ databases. RL BC Cancer Agency, Canada's Michael Smith Genome Sciences Centre, Suite 100, RL 570 West 7th Avenue, Vancouver, British Columbia V5Z 4S6, Canada XX DR Ensembl-Gn; ENSBTAG00000019852; Bos_taurus. DR Ensembl-Tr; ENSBTAT00000026448; Bos_taurus. XX CC Contact: Robert Kirkpatrick CC Canada's Michael Smith Genome Sciences Centre CC BC Cancer Agency CC Suite 100, 570 West 7th Avenue, Vancouver, British Columbia, CC Canada, V5Z 4S6 CC Tel: 1-604-707-5900 x5406 CC Fax: 1-604-876-3561 CC Email: robertk@bcgsc.ca CC Tissue Procurement: M. Taniguchi, Y. Meng, S. Lobo, L. Guan and S. CC Moore CC cDNA Library Preparation: M. Masaaki, Y. Meng, S. Lobo, L. Guan CC and Dr. S. Moore CC cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) CC DNA Sequencing by: Bovine Genome Sequencing Program, Genome CC Sequence Centre, CC BC Cancer Agency, Vancouver, BC, Canada CC info@bcgsc.bc.ca CC Moore S, Alexander L, Brownstein M, Guan L, Lobo S, Meng Y, CC Tanaguchi M, Wang Z, Prange C, Schreiber K, Shenmen C, Wagner L, CC Ali J, Chun E, Liao N, Beland J, Cruz K, Featherstone R, Kirk H, CC Matsuo C, Mayo M, Moore R, Munro S, Roger J, Tam B, Trinh E, Sze W, CC Wilton J, Wanger S, Huang P, Chu B, Imanian B, Roscoe R, Holt R, CC Jones S, Marra M CC Clone distribution: MGC clone distribution information can be found CC through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov CC Series: IRAK Plate: 309 Row: i Column: 9. XX FH Key Location/Qualifiers FH FT source 1..4180 FT /organism="Bos taurus" FT /lab_host="DH10B" FT /strain="Hereford" FT /mol_type="mRNA" FT /clone_lib="GC_BGC-11" FT /clone="MGC:165778 IMAGE:8415761" FT /tissue_type="Hypothalamus, 8.5 month female Hereford" FT /note="Vector: pCMV-SPORT6.0" FT /db_xref="taxon:9913" FT gene 1..4180 FT /gene="PDHA1" FT /note="synonym: PDHA" FT CDS 107..1279 FT /codon_start=1 FT /gene="PDHA1" FT /product="PDHA1 protein" FT /db_xref="GOA:A7MB35" FT /db_xref="InterPro:IPR017597" FT /db_xref="UniProtKB/Swiss-Prot:A7MB35" FT /protein_id="AAI51314.1" FT /translation="MRKMLAAVSRVLSGVAQKPASRVLVASRHFANDATFEIKKCDLHR FT LEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGL FT EAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRRGGCAKGKGGSMHMYAKNFYGG FT NGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFIC FT ENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATKFAAAYCRSGKGPIL FT MELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDV FT EVRKEIEDAAQFATADPEPPLEELGYHIYCNDPPFEVRGANQWIKFKSIS" XX SQ Sequence 4180 BP; 1125 A; 916 C; 944 G; 1195 T; 0 other; tgaagtgtaa acgtcagctc agggcacctg aaggagactt ggacgcacct gggccgcact 60 cctgggctcg ttgaaggttc gcagctgcag ccactgcctg cgctccatga ggaagatgct 120 cgccgccgtc tctcgcgtgt tgtcgggcgt cgcccagaag ccggcaagca gagtgctggt 180 ggcatctcgt cattttgcaa atgatgctac ttttgaaatt aagaaatgtg atcttcaccg 240 gctggaagag ggccctcctg tcactacagt gcttaccagg gaggatgggc tcaaatacta 300 caggatgatg cagaccgttc gccgaatgga gttaaaagca gatcagctgt ataaacagaa 360 aattattcgt ggtttctgtc acttgtgtga tggtcaggaa gcttgttgtg taggcctgga 420 ggccggcatc aaccccacag accatctgat cacagcgtac cgggctcatg gctttacctt 480 tactcgtgga ctctctgtcc gggagatcct tgcagagctt acaggacgaa gaggtggttg 540 tgctaaagga aaaggaggat caatgcatat gtatgccaag aatttctacg gaggcaatgg 600 catcgtggga gctcaggtgc ccctgggagc tgggattgct ctggcctgta agtataatgg 660 aaaagacgag gtctgtttga ctttgtacgg tgatggcgct gctaatcagg gtcagatatt 720 cgaagcttac aatatggcag ctttgtggaa attgccttgt attttcatct gtgagaataa 780 ccgctatggg atgggaacat ctgtggaaag agcagcagcc agcactgatt actacaagag 840 aggcgacttt attcctgggc tgagggtaga tggaatggat atcctgtgtg tccgggaggc 900 cacaaaattt gcagctgcct attgtagatc tggaaagggg cctatactga tggagctgca 960 gacttaccgt taccatggac acagcatgag tgatcctgga gtgagttacc gtacccgaga 1020 agagattcag gaagttagaa gtaagagtga ccctatcatg ctcctcaagg acaggatggt 1080 gaacagcaat ctcgccagtg ttgaagaact aaaggaaatt gatgtggaag tgagaaaaga 1140 aattgaggat gctgctcagt ttgctactgc tgatcctgaa ccacctttgg aagaactcgg 1200 ctatcacatt tactgcaacg atccaccttt tgaagtccgg ggtgcaaacc agtggatcaa 1260 gtttaagtcc atcagttaag gcgagataca cacaccttta gtgggttatt tggcagcaac 1320 tgtaaggaat gctttgtgtt ctcaactttg tcaaggagga aaacccatag agagaaagcg 1380 ctgtagattg agatagtgtt tgcatgtggt ttgtacagtg ttgcattaag agaggggatt 1440 gcacagataa ggttatttta gttatatagg tctaaaatag gtatagtttg cttaaccctc 1500 caaattattt ctttaaaata cttctatttg atttcattgt atactagttt taacacttcc 1560 cttgcagctc agctggtaaa gaatctgcct gcaatgtagg agatcccggt tcgattcctg 1620 ggttgggaag atcccctgga gaagggatag gctacccact ccagtattct tgggcttccc 1680 ttgtggctca gctgttaaag aatctgcctg caatgcagga gacctgggtt caatccctgg 1740 attgggaaga tcccctggag aattccatgg actgtatagt ccatggggtc acaaagagtt 1800 ggacacgagt gagcaacttt gattttaact ttactaaaag gaaaaacaat tccttgtatg 1860 cctgtccaac tctgccgcca ccttgtggga ggatcagtgt cctgtccctt gcagtgccca 1920 atagcttacc tcagttcagt tcagtcactc agttgtgtcc gactcttcgt gaccccatga 1980 attgcagcac gccaggcctc cctgtcctca ccaactcccg gagttcaccg aaacccaggt 2040 ccatcgagtc ggtgatgcca tccagccatc tcatcctctg tcgtcccctt ctcctcctgc 2100 ccccaatccc tcccagcatc tgagtctttt ccaatgagtc aactcttcgc atgaggtggc 2160 caaagtactg gagtttcagc tttagcatca ttccttccaa agaacaccca ggactgatct 2220 cctttagaat ggactggttg gatcgccttg cagtctaagg gactctcaag actcttctcc 2280 aacaccacag ttcaaaagca tcaattcttc ggtcctcagc tttcttcaca gtccaagtcc 2340 cacatccaca catgactact ggaaaaacca tagccttgac tagatggacc tttgttggca 2400 aaataacgtc tctgcttttg aatatgctat ctaggttggt cataactttc cttccaagga 2460 gtaagcgtct tttaattaac agcttaccta gtgtacccta atgtgcacac acgcccctgt 2520 tagtgtgcac gtcggtgctg aagcctgttc acactgaacc atcatgtctc attaataaaa 2580 cacaattatt tattataact tggcaggggt ggaaggacaa ttctacttca tttcaaaggc 2640 caaataagtt agttttctcc atttatacat tcaatataaa tatgaagcta ttttcttaac 2700 agttttctat ttacattcat atcaaacatt attaaataca aggcatagtc ttatcgattt 2760 tatctttttc taattttaag tgcaattcca gttaatgtat ggaatgtata attgaaatag 2820 tgattccgag cctttttgtt aaacacctat tgttcaacct ttccagcaaa tgtttgaagt 2880 taaagaatcc cataatactt tgggatgtca agaccaagtt ttacaatcac agttctcaaa 2940 atacatacag ttctactcac ctttaaaaca tgttgagaaa tattaacatt ttttccataa 3000 ttctttaaga acttgaagtt ctaaatttgt tcagtgaaca gtggtgtttt ccttgtggaa 3060 tcgcagcagg attggtgtgt atgtaacaga agctttaaca aagatgtcag ctctgggctc 3120 cctctatttc ctgcgtggct gattattaag cctcatcttg ggttgctggc gccccctgag 3180 cccgtcttct gtactgggag actgcagtcc agagcctgca gagaagacca ccgctacgaa 3240 acaaacagaa gtcttcttta gagtgagtag taaggaaaga tgacttcaaa gcaataccac 3300 acaatacttc tttaggagcc cttatggcaa aagctatagt atgtacttct agacttgttt 3360 tgctggctag tcttaaaaat gcagtcccta acttaaaaac taaaatagga atatgttccc 3420 ttcacatatg gaagtgaaat ttctacccca gcacgagtgg gatgtggcct tagaggtatg 3480 tacaggagtg ccagggcacc tgtgcaaagt aggctattaa agatgaccag tggaggagaa 3540 tcaaagggtt tgagtgctgt cagctcctgt ctttgctcat tccactccca gcctggcagt 3600 ctgttgactt tgcttactgg gcctttagct tctgctccct ctcagctttt ccccagcttc 3660 acccttggaa actccagagt ttggagtacg gttgggtagt ttttgtttcc tgactagggg 3720 aaagggggaa agctaccagc actttctttt ctactgcttt tatgtaaaag cctaactaca 3780 cccaaaaagc cattttctgt tacacaaaat gttttgaggc tattcttacc gtagtctaag 3840 gtaccttagg tcttccttag tgatatcatt aagaatatca gacagtgtgt aaccctcttc 3900 aagaatctga aacaaagatg acatacatca agagctaagc agctttgtaa taacagccta 3960 ggaatggttt ggttctcagc ttaccttttc aattgttctc gtatccgctc cttgcagttg 4020 caaccaatcg acaagctctt tatctgttcg ctgctcctga gacccaggag gggatactga 4080 gttctctgta atgattagaa atgtgttatg ttgtatactg atgtgtaaca gtaaaagatg 4140 gatgaaataa actattccag aatgaaaaaa aaaaaaaaaa 4180 // |
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