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EBI DbfetchID BC122039; SV 1; linear; mRNA; STD; VRT; 1242 BP. XX AC BC122039; XX DT 17-AUG-2006 (Rel. 88, Created) DT 19-MAR-2009 (Rel. 100, Last updated, Version 3) XX DE Xenopus tropicalis 3-oxo-5-alpha-steroid 4-dehydrogenase 3, mRNA (cDNA DE clone MGC:147215 IMAGE:7826874), complete cds. XX KW MGC. XX OS Xenopus (Silurana) tropicalis (western clawed frog) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia; OC Batrachia; Anura; Mesobatrachia; Pipoidea; Pipidae; Xenopodinae; Xenopus; OC Silurana. XX RN [1] RP 1-1242 RX DOI; 10.1002/dvdy.10174 RX PUBMED; 12454917. RA Klein S.L., Strausberg R.L., Wagner L., Pontius J., Clifton S.W., RA Richardson P.; RT "Genetic and genomic tools for Xenopus research: The NIH Xenopus RT initiative"; RL Dev. Dyn. 225(4):384-391(2002). XX RN [2] RP 1-1242 RX DOI; 10.1073/pnas.242603899 RX PUBMED; 12477932. RG Mammalian Gene Collection Program Team RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., RA Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., RA Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., RA Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K., RA Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F., RA Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S., RA Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J., RA Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J., RA Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M., RA Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., RA Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., RA Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C., RA Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I., RA Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human and RT mouse cDNA sequences"; RL Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002). XX RN [3] RC NIH-MGC Project RP 1-1242 RA Klein S., Gerhard D.S.; RT ; RL Submitted (15-AUG-2006) to the EMBL/GenBank/DDBJ databases. RL National Institutes of Health, Xenopus Gene Collection (XGC), National RL Institute of Child Health and Human Development, 6100 Executive Boulevard, RL Room 4B01, Rockville, MD 20892-7510, USA XX DR ImaGenes; IMAGp998M1616606Q. DR ImaGenes; IRAKp961I16279Q. DR ImaGenes; IRBNp992F0739D. XX CC Contact: XGC help desk CC Email: cgapbs-r@mail.nih.gov CC Tissue Procurement: Robert M. Grainger CC cDNA Library Preparation: Bruce Blumberg Laboratory, University of CC California, Irvine CC cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) CC DNA Sequencing by: Sequencing Group at the Stanford Human Genome CC Center, Stanford University School of Medicine, Stanford, CA 94305 CC Web site: http://www-shgc.stanford.edu CC Contact: (Dickson, Mark) mcd@paxil.stanford.edu CC Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers, CC R. M. CC Clone distribution: MGC clone distribution information can be found CC through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov CC Series: IRAK Plate: 279 Row: i Column: 16 CC This clone was selected for full length sequencing because it CC passed the following selection criteria: matched mRNA gi: CC 112418645. XX FH Key Location/Qualifiers FH FT source 1..1242 FT /organism="Xenopus (Silurana) tropicalis" FT /lab_host="ElectroMAX DH10B T1 Phage Resistent cells" FT /strain="N6 (Nigerian 6th generation inbred)" FT /mol_type="mRNA" FT /clone_lib="NIH_XGC_tropOva1" FT /clone="MGC:147215 IMAGE:7826874" FT /tissue_type="Ovary, adult female, strain N6 (Nigerian 6th FT generation inbred)" FT /note="Vector: pCS107" FT /db_xref="taxon:8364" FT gene 1..1242 FT /gene="srd5a3" FT CDS 186..1112 FT /codon_start=1 FT /gene="srd5a3" FT /product="3-oxo-5-alpha-steroid 4-dehydrogenase 3" FT /db_xref="GOA:Q0P4J9" FT /db_xref="InterPro:IPR001104" FT /db_xref="UniProtKB/Swiss-Prot:Q0P4J9" FT /protein_id="AAI22040.1" FT /translation="MTLLALVWLLLDATFLITLLWHLLQGCKSGHSLLCSVFQDLIRYG FT KTKTGLQRPAWLQWFDIPKRCFWHFYCVSLIWNGCLLWILLRLLLQSVPVPEWLQLVLH FT FLHAGSEPQILDRELSVILALALLWLHSLRRLLECLFVSVFSNGVIHLVQYCFGLGYYF FT LIGITVLTYCPLDRRTVSTDNLLTQCHWYHILGLALYIWASLHQYRCHCILAGLRKSAS FT GNVINLNHSVPCGDWFERVSCPHYFAELLIYVSIAVVFGLLNTIWWLVVLYVLLNQALA FT ALLCHEFYHEKFDTYPIHRKAFIPFIF" XX SQ Sequence 1242 BP; 297 A; 269 C; 280 G; 396 T; 0 other; ggatatatga ataagataac cagcaggggg cagcagagta cagccatcta atacgctata 60 gtggaacggt atatccccgc cctactttct ccatatgggt gtggcttatg ggatgacaga 120 tgctcactgg cagagaggat gacacatggt agaacatgca gcatgtacag ctatttccgc 180 caggaatgac cctactggcc ctagtttggc ttctgctgga tgccaccttc cttatcaccc 240 tgctgtggca cctgctacag ggatgtaaga gtgggcactc ccttttatgc agtgtcttcc 300 aggatctaat acgctatggg aagactaaaa ctggcttaca gcggccagca tggctccagt 360 ggtttgatat cccaaagcgg tgcttctggc atttttattg tgtctctctg atctggaatg 420 ggtgtctgct gtggatcctc cttcgcttac tgctccaaag tgttccagtt ccagaatggc 480 tgcaactggt gcttcatttc ctgcatgccg gatcagaacc acaaatcctg gatagagagc 540 tttcagttat tctggctctt gctctgttgt ggttgcacag cctgcggaga ttgctggagt 600 gtctgtttgt gagtgtcttt tccaatgggg tcattcactt ggtgcagtac tgctttggct 660 tgggctatta cttcttgatt gggatcactg tactcaccta ttgcccactg gacagaagga 720 ctgtctccac ggacaatctt ctaacgcagt gtcactggta ccatatccta ggactggcac 780 tctatatctg ggcttctcta caccaataca gatgtcattg tattcttgct ggtctgagga 840 aaagtgcatc agggaacgta ataaacctga accattctgt gccctgcgga gactggtttg 900 agagagtgtc ctgccctcat tactttgcag agctcttgat atatgtgtcc atcgctgttg 960 tttttggact tctgaacact atatggtggc tggttgtttt atatgtttta cttaaccagg 1020 cattagctgc acttttgtgt catgagtttt atcatgaaaa gtttgataca tatcctattc 1080 acaggaaagc tttcattcca tttatatttt gaatataact gtatactata tatttccata 1140 tatatgcctc ttttgctttc tctgtttcag tacttaagta agtgtattta tccttgatat 1200 ggcaatacaa gagaatatag tttcaaaaaa aaaaaaaaaa aa 1242 // |
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