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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   BC118238; SV 1; linear; mRNA; STD; MAM; 1446 BP.
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AC   BC118238;
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DT   27-JUN-2006 (Rel. 88, Created)
DT   12-JUN-2008 (Rel. 96, Last updated, Version 6)
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DE   Bos taurus thiopurine S-methyltransferase, mRNA (cDNA clone MGC:139740
DE   IMAGE:8281873), complete cds.
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KW   MGC.
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OS   Bos taurus (cattle)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
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RN   [1]
RC   NIH-MGC Project
RP   1-1446
RA   Moore S., Alexander L., Brownstein M., Guan L., Lobo S., Meng Y.,
RA   Tanaguchi M., Wang Z., Yu J., Prange C., Schreiber K., Shenmen C.,
RA   Wagner L., Bala M., Barbazuk S., Barber S., Babakaiff R., Beland J.,
RA   Chun E., Del Rio L., Gibson S., Hanson R., Kirkpatrick R., Liu J.,
RA   Matsuo C., Mayo M., Santos R.R., Stott J., Tsai M., Wong D., Siddiqui A.,
RA   Holt R., Jones S.J., Marra M.A.;
RT   ;
RL   Submitted (23-JUN-2006) to the EMBL/GenBank/DDBJ databases.
RL   BC Cancer Agency, Canada's Michael Smith Genome Sciences Centre, Suite 100,
RL   570 West 7th Avenue, Vancouver, British Columbia V5Z 4S6, Canada
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DR   Ensembl-Gn; ENSBTAG00000019300; Bos_taurus.
DR   Ensembl-Tr; ENSBTAT00000025698; Bos_taurus.
DR   ImaGenes; IMAGp998K2317945Q.
DR   ImaGenes; IRAKp961C03269Q.
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CC   Contact: Robert Kirkpatrick
CC   Canada's Michael Smith Genome Sciences Centre
CC   BC Cancer Agency
CC   Suite 100, 570 West 7th Avenue, Vancouver, British Columbia,
CC   Canada, V5Z 4S6
CC   Tel: 1-604-707-5900 x5406
CC   Fax: 1-604-876-3561
CC   Email: robertk@bcgsc.ca
CC   Tissue Procurement: Lee Alexander, USDA, ARS, NPA
CC   cDNA Library Preparation: Express Genomics
CC   cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
CC   DNA Sequencing by: Bovine Genome Sequencing Program, Genome
CC   Sequence Centre,
CC   BC Cancer Agency, Vancouver, BC, Canada
CC   info@bcgsc.bc.ca
CC   Moore S, Alexander L, Brownstein M, Guan L, Lobo S, Meng Y,
CC   Tanaguchi M, Wang Z, Prange C, Schreiber K, Shenmen C, Wagner L,
CC   Ali J, Chun E, Liao N, Beland J, Cruz K, Featherstone R, Kirk H,
CC   Matsuo C, Mayo M, Moore R, Munro S, Roger J, Tam B, Trinh E, Sze W,
CC   Wilton J, Wanger S, Huang P, Chu B, Imanian B, Roscoe R, Holt R,
CC   Jones S, Marra M
CC   Clone distribution: MGC clone distribution information can be found
CC   through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
CC   Series: IRAK Plate: 269 Row: c Column: 3.
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FH   Key             Location/Qualifiers
FH
FT   source          1..1446
FT                   /organism="Bos taurus"
FT                   /lab_host="DH10B"
FT                   /strain="L1 Hereford"
FT                   /mol_type="mRNA"
FT                   /clone_lib="GC_BGC-27"
FT                   /clone="MGC:139740 IMAGE:8281873"
FT                   /tissue_type="Basal ganglia, female calf 8.5 months, L1
FT                   Hereford"
FT                   /note="Vector: pExpress1"
FT                   /db_xref="taxon:9913"
FT   gene            1..1446
FT                   /gene="TPMT"
FT   CDS             92..829
FT                   /codon_start=1
FT                   /gene="TPMT"
FT                   /product="thiopurine S-methyltransferase"
FT                   /db_xref="GOA:Q17QQ2"
FT                   /db_xref="InterPro:IPR016822"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q17QQ2"
FT                   /protein_id="AAI18239.1"
FT                   /translation="MGDSRALLDSEEYPNTEAQKDRVLTLEEWQEKWVNHKTGFHQEQG
FT                   HQLLKKYLDTFLKGEKALRVFFPLCGKAVEMKWFADRGHSVVGVEISELGIRDFFTEQN
FT                   LSYSEEPIMEIPGAKIFKSSSGNISLYCCNLFDLPRANIGKFDRIWDRGALVAVNPSDR
FT                   KRYSDVMLSLTRPGFRYLLSVFSYDPTKHAGPPFYVTDGEVKKLFGSVCNIQCLEKVDV
FT                   FEERHKSWGIDQIIERLYLFTEK"
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SQ   Sequence 1446 BP; 522 A; 222 C; 293 G; 409 T; 0 other;
     ggcgggcgga cagcgcacga ggtaggggcc gccgggcgcg gaggaaggca ctggggacat        60
     ttgtttgtga gccagagcca tctgcaaaac catgggtgat tcaagagcct tacttgacag       120
     tgaagagtac ccaaacactg aggctcagaa agaccgagtg ctgactctgg aagaatggca       180
     agaaaagtgg gtgaaccaca aaactggatt tcatcaagaa caaggacatc agctattaaa       240
     gaagtatttg gatacctttc ttaaaggcga gaaagcactg cgggtatttt ttcccctttg       300
     tggaaaagca gttgagatga aatggtttgc agaccgagga cacagtgtag taggcgtgga       360
     aatcagcgaa cttgggatac gggacttttt tacggagcag aatctttctt actcagaaga       420
     accaattatg gaaattcctg gagccaaaat attcaagagt tcttcaggga acatttcatt       480
     gtattgctgc aacctttttg atcttcccag agcaaatatt ggcaaatttg ataggatttg       540
     ggacagagga gccttagtcg ctgttaatcc aagtgatcgc aaacgctact cagatgtaat       600
     gttgtcccta acgagacctg ggtttcggta cctcttgtct gttttttctt acgatccaac       660
     caaacatgca ggtccaccgt tttacgttac agatggggaa gttaaaaaat tgttcggttc       720
     agtatgcaat attcaatgtc tagagaaggt tgatgttttt gaagaacgac ataaaagttg       780
     gggaatcgac cagattattg aaaggttata tctatttaca gaaaagtaaa tgagacctaa       840
     atagaagcaa ataatatcaa aatgtttttg aacaattgaa aattgtgcta aggcatgaaa       900
     atggatgact tttgcaggaa aaattgttca caaatcatat cacagatctt tgctaaaaaa       960
     atgcttataa ctttttagaa aaagcacata ttttggaaag gagtgtatga ctgagaataa      1020
     aatattgtac ttgtatgtct tacagattgt aagaattttt tacaatcagc agatattact      1080
     catataatta aaaaagaaaa aagaaatgca atgtatgtaa caaatataat agaaaaatgg      1140
     tatctttatg aagtttagaa gcactgagga gatgcagcac ttttaacagg gttcaaggta      1200
     aaagctaacc atctaagaca ttataataaa acattaattt ttactataaa ccctcaaatt      1260
     gtactaaatc ctagcattcc ttttcatatt tttcttctta tataatttta tatgcgacag      1320
     aatgaggaaa aaaattttta actaatgaaa ttgaccactc tatatggaac aaaatatttt      1380
     tctcaaactg taaaaataaa tcatttttag aagttttaaa aaaaaaaaaa aaaaaaaaaa      1440
     aaaaaa                                                                 1446
//
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