EBI Dbfetch

ID   BC111139; SV 1; linear; mRNA; STD; MAM; 1606 BP.
XX
AC   BC111139;
XX
DT   10-DEC-2005 (Rel. 86, Created)
DT   12-JUN-2008 (Rel. 96, Last updated, Version 6)
XX
DE   Bos taurus thymidylate synthetase, mRNA (cDNA clone MGC:134366
DE   IMAGE:8056801), complete cds.
XX
KW   MGC.
XX
OS   Bos taurus (cattle)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
XX
RN   [1]
RC   NIH-MGC Project
RP   1-1606
RA   Moore S., Alexander L., Brownstein M., Guan L., Lobo S., Meng Y.,
RA   Tanaguchi M., Wang Z., Yu J., Prange C., Schreiber K., Shenmen C.,
RA   Wagner L., Bala M., Barbazuk S., Barber S., Babakaiff R., Beland J.,
RA   Chun E., Del Rio L., Gibson S., Hanson R., Kirkpatrick R., Liu J.,
RA   Matsuo C., Mayo M., Santos R.R., Stott J., Tsai M., Wong D., Siddiqui A.,
RA   Holt R., Jones S.J., Marra M.A.;
RT   ;
RL   Submitted (07-DEC-2005) to the EMBL/GenBank/DDBJ databases.
RL   BC Cancer Agency, Canada's Michael Smith Genome Sciences Centre, Suite 100,
RL   570 West 7th Avenue, Vancouver, British Columbia V5Z 4S6, Canada
XX
DR   Ensembl-Gn; ENSBTAG00000007003; Bos_taurus.
DR   Ensembl-Tr; ENSBTAT00000009213; Bos_taurus.
DR   ImaGenes; IMAGp998E2317310Q.
DR   ImaGenes; IRAKp961M21258Q.
XX
CC   Contact: Robert Kirkpatrick
CC   Canada's Michael Smith Genome Sciences Centre
CC   BC Cancer Agency
CC   Suite 100, 570 West 7th Avenue, Vancouver, British Columbia,
CC   Canada, V5Z 4S6
CC   Tel: 1-604-707-5900 x5406
CC   Fax: 1-604-876-3561
CC   Email: robertk@bcgsc.ca
CC   Tissue Procurement: Y. Meng, M. Taniguchi, L. Guan and S. Moore
CC   cDNA Library Preparation: M. Masaaki, Y. Meng, S. Lobo,  L. Guan
CC   and Dr. S. Moore
CC   cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
CC   DNA Sequencing by: Bovine Genome Sequencing Program, Genome
CC   Sequence Centre,
CC   BC Cancer Agency, Vancouver, BC, Canada
CC   info@bcgsc.bc.ca
CC   Moore S, Alexander L, Brownstein M, Guan L, Lobo S, Meng Y,
CC   Tanaguchi M, Wang Z, Prange C, Schreiber K, Shenmen C, Wagner L,
CC   Ali J, Chun E, Liao N, Beland J, Cruz K, Featherstone R, Kirk H,
CC   Matsuo C, Mayo M, Moore R, Munro S, Roger J, Tam B, Trinh E, Sze W,
CC   Wilton J, Wanger S, Huang P, Chu B, Imanian B, Roscoe R, Holt R,
CC   Jones S, Marra M
CC   Clone distribution: MGC clone distribution information can be found
CC   through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
CC   Series: IRAK Plate: 258 Row: m Column: 21.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1606
FT                   /organism="Bos taurus"
FT                   /lab_host="ElectroMAX DH10B"
FT                   /strain="Crossbred x Angus"
FT                   /mol_type="mRNA"
FT                   /clone_lib="GC_BGC-04"
FT                   /clone="MGC:134366 IMAGE:8056801"
FT                   /tissue_type="Liver, 6 month old female calf, Crossbred x
FT                   Angus"
FT                   /note="Vector: pCMV-SPORT6.0"
FT                   /db_xref="taxon:9913"
FT   gene            1..1606
FT                   /gene="TYMS"
FT                   /note="synonym: TS"
FT   CDS             90..1154
FT                   /codon_start=1
FT                   /gene="TYMS"
FT                   /product="thymidylate synthetase"
FT                   /db_xref="GOA:Q2TA32"
FT                   /db_xref="InterPro:IPR000398"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q2TA32"
FT                   /protein_id="AAI11140.1"
FT                   /translation="MPAAGSEPSRPPSPPGVQEQSAEPRPPPPPHGELQYLGQIEHILR
FT                   CGFRRDDRTGTGTLSVFGMQARYNLRDEFPLLTTKRVFWKGVLEELLWFIKGSTNAKEL
FT                   SSKGVKIWDANGSRDFLDGLGFSDRAEGDLGPVYGFQWRHFGAEYKDMDSEYSGQGVDQ
FT                   LQKVIDTIKTNPNDRRIILCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDM
FT                   GLGVPFNIASYALLTYMIAHITDLKPGDFVHTLGDAHIYLNHIEPLKTQALMELRGQSS
FT                   RSLDGDGQAGTSRWAPVATDTERDRCCELQREPRPFPKLKILRKVETIDDFQAEDFQIE
FT                   GYNPNPTIKMEMAV"
XX
SQ   Sequence 1606 BP; 416 A; 387 C; 426 G; 377 T; 0 other;
     ggtgtggcgg gaaaagagac tcctgggaag cgctgggagg ccgttcccgc ctccgtacgg        60
     ccctcgccgc cgcgcccgca accagcacta tgccagcggc cggctccgag ccgtcgcgcc       120
     cgccgtcgcc gcccggcgtg caggagcaga gcgccgagcc gcggccgccg ccgccgccgc       180
     acggggagct gcagtacctg gggcagatag aacacatcct ccgctgcggc ttccggaggg       240
     atgaccgcac cggcactggc accctgtcgg tgttcgggat gcaggcgcgg tacaacttga       300
     gagatgaatt tcctctgctg acaaccaaac gtgttttctg gaaaggtgtt ttggaggagt       360
     tgctgtggtt tatcaaggga tccaccaacg ctaaggaact ctcttccaag ggagtgaaaa       420
     tttgggatgc caatgggtcc cgagacttct tggatggcct gggcttctcc gacagagctg       480
     aaggggattt aggcccagtt tatggcttcc agtggaggca ttttggggct gaatacaaag       540
     atatggattc agagtattca ggtcaaggag tagatcaact gcaaaaagtg atcgacacaa       600
     tcaaaaccaa ccctaacgac agaagaatca tcctgtgtgc ttggaatcca aaagatctgc       660
     ctctcatggc cctcccccca tgccacgccc tctgccagtt ctacgtggtg aatggggagt       720
     tgtcctgcca gttgtaccag cggtcggggg acatgggcct gggtgtgccc ttcaacattg       780
     ccagctacgc cctgctcacc tacatgatcg cacacatcac ggacctgaag ccaggtgact       840
     tcgtgcacac tctgggagat gcacacattt acctgaatca catcgagcca ctgaaaactc       900
     aggctctgat ggagctaaga ggacagtctt ccaggtctct ggatggtgat ggacaggcag       960
     ggaccagcag atgggcccca gtggccacag acactgaacg tgacaggtgc tgtgagctgc      1020
     agcgagaacc aaggcctttc cccaagctca aaatccttcg aaaagttgag acaatcgatg      1080
     acttccaggc cgaagacttt cagattgaag gctacaatcc taatccgact attaaaatgg      1140
     aaatggctgt ctagagtgct tttaaaggca ttgtttgaag gatatttaca cccaggtaaa      1200
     agttcactgg gctctaaagg cgaaggatct aggccaaaac gtgttagtga tctgttggtt      1260
     gttatccatt aacttttaag aatgttgcca ctggcaaata agagctgtgc tgttctctta      1320
     gtaataaaag gccttgggtt tgggtgactc actgagggtg tctgtacata cagtgatttt      1380
     gaataaagac aggaaagaga atttatatat attaattcaa atttcataga gtttttagga      1440
     aaatggtcag tgcatttcaa gaattatata cactatttcc ctaaatctga gcaagctggg      1500
     taacacccat ccgtcatgtt aatgcctagt gtggttatga acgtttaaag ttttatatct      1560
     tgctacaata aatgttctgc gttcatgttt aaaaaaaaaa aaaaaa                     1606
//