EBI Dbfetch

ID   BC105543; SV 1; linear; mRNA; STD; MAM; 1853 BP.
XX
AC   BC105543;
XX
DT   23-FEB-2006 (Rel. 86, Created)
DT   21-OCT-2008 (Rel. 97, Last updated, Version 6)
XX
DE   Bos taurus 3-hydroxyisobutyrate dehydrogenase, mRNA (cDNA clone MGC:128999
DE   IMAGE:8120949), complete cds.
XX
KW   MGC.
XX
OS   Bos taurus (cattle)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
XX
RN   [1]
RC   NIH-MGC Project
RP   1-1853
RA   Moore S., Alexander L., Brownstein M., Guan L., Lobo S., Meng Y.,
RA   Tanaguchi M., Wang Z., Yu J., Prange C., Schreiber K., Shenmen C.,
RA   Wagner L., Bala M., Barbazuk S., Barber S., Babakaiff R., Beland J.,
RA   Chun E., Del Rio L., Gibson S., Hanson R., Kirkpatrick R., Liu J.,
RA   Matsuo C., Mayo M., Santos R.R., Stott J., Tsai M., Wong D., Siddiqui A.,
RA   Holt R., Jones S.J., Marra M.A.;
RT   ;
RL   Submitted (20-SEP-2005) to the EMBL/GenBank/DDBJ databases.
RL   BC Cancer Agency, Canada's Michael Smith Genome Sciences Centre, Suite 100,
RL   570 West 7th Avenue, Vancouver, British Columbia V5Z 4S6, Canada
XX
DR   Ensembl-Gn; ENSBTAG00000001036; Bos_taurus.
DR   Ensembl-Tr; ENSBTAT00000001374; Bos_taurus.
DR   ImaGenes; IMAGp998F1917476Q.
DR   ImaGenes; IRAKp961H19252Q.
XX
CC   Contact: Robert Kirkpatrick
CC   Canada's Michael Smith Genome Sciences Centre
CC   BC Cancer Agency
CC   Suite 100, 570 West 7th Avenue, Vancouver, British Columbia,
CC   Canada, V5Z 4S6
CC   Tel: 1-604-707-5900 x5406
CC   Fax: 1-604-876-3561
CC   Email: robertk@bcgsc.ca
CC   Tissue Procurement: Lee Alexander, USDA, ARS, NPA
CC   cDNA Library Preparation: Express Genomics
CC   cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
CC   DNA Sequencing by: Bovine Genome Sequencing Program, Genome
CC   Sequence Centre,
CC   BC Cancer Agency, Vancouver, BC, Canada
CC   info@bcgsc.bc.ca
CC   Moore S, Alexander L, Brownstein M, Guan L, Lobo S, Meng Y,
CC   Tanaguchi M, Wang Z, Prange C, Schreiber K, Shenmen C, Wagner L,
CC   Ali J, Chun E, Liao N, Beland J, Cruz K, Featherstone R, Kirk H,
CC   Matsuo C, Mayo M, Moore R, Munro S, Roger J, Tam B, Trinh E, Sze W,
CC   Wilton J, Wanger S, Huang P, Chu B, Imanian B, Roscoe R, Holt R,
CC   Jones S, Marra M
CC   Clone distribution: MGC clone distribution information can be found
CC   through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
CC   Series: IRAK Plate: 252 Row: h Column: 19.
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FH   Key             Location/Qualifiers
FH
FT   source          1..1853
FT                   /organism="Bos taurus"
FT                   /lab_host="DH10B"
FT                   /strain="L1 Hereford"
FT                   /mol_type="mRNA"
FT                   /clone_lib="GC_BGC-17"
FT                   /clone="MGC:128999 IMAGE:8120949"
FT                   /tissue_type="Ascending colon, 6 month male fetus, L1
FT                   Hereford"
FT                   /note="Vector: pExpress1"
FT                   /db_xref="taxon:9913"
FT   gene            1..1853
FT                   /gene="HIBADH"
FT   CDS             3..1013
FT                   /codon_start=1
FT                   /gene="HIBADH"
FT                   /product="3-hydroxyisobutyrate dehydrogenase"
FT                   /db_xref="GOA:Q2HJD7"
FT                   /db_xref="InterPro:IPR016040"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q2HJD7"
FT                   /protein_id="AAI05544.1"
FT                   /translation="MAASLRLRGAASGLRYWSRRQPPAVASLAAVCSRSMASKTPVGFI
FT                   GVGNMGNPMAKNLMKHGYPLIIYDVFPDACKEFLDAGEQVVSSPADVAEKADRIITMLP
FT                   TSINAIEAYSGANGILKKVKKGSLLIDSSTIDPMVSKELAKEVEKMGAVFMDAPVSGGV
FT                   GAARSGNLTFMVGGVEEEFAAAQELLGCMGSNVVYCGAVGTGQAAKICNNLLLAISMIG
FT                   TAEAMNLGIRLGLDPKLLAKILNMSSGRCWSSDTYNPVPGVMDGVPSANNYQGGFGTTL
FT                   MAKDLGLAQDSATSTKSPILLGSQAHQIYRMMCAKGYSKKDFSSVFQFLREEETF"
XX
SQ   Sequence 1853 BP; 526 A; 372 C; 407 G; 548 T; 0 other;
     gcatggcagc ctccttacgg ctccgtggag ccgcctccgg cctccggtat tggagccgcc        60
     ggcagcctcc ggcagtggcc agccttgcag cggtgtgttc taggtcaatg gcttctaaga       120
     ctccagttgg attcattgga gttggcaaca tggggaatcc aatggcaaaa aatctcatga       180
     aacatggcta tccacttatt atttatgatg tgttccctga tgcttgcaaa gagtttctag       240
     atgcaggtga acaggtagtg tcttccccag cagatgtagc tgaaaaagct gacagaatta       300
     ttaccatgtt gcccaccagc atcaatgcga tagaagctta ttctggagca aatggaattc       360
     taaaaaaagt gaagaaaggc tcactattaa tagattccag tactattgat cctatggttt       420
     caaaagaact ggccaaagaa gttgaaaaaa tgggagcagt tttcatggat gcccctgttt       480
     ctggtggtgt gggagccgct cggtcgggga acctcacatt tatggtagga ggagtcgaag       540
     aagaatttgc tgctgcccaa gagttgctgg ggtgcatggg ctccaatgtg gtgtattgtg       600
     gagctgttgg gacagggcag gctgcgaaga tctgcaacaa cttgctgtta gctattagta       660
     tgattggaac tgctgaagct atgaatctgg gaatcaggtt agggcttgac ccaaaactct       720
     tggctaaaat cctaaatatg agctcaggac gatgttggtc aagtgacact tataatcctg       780
     tacctggggt gatggatgga gttccctcgg ctaataacta tcagggcgga ttcggaacaa       840
     cactcatggc gaaggatctg ggattagcac aagactctgc caccagcacg aagagcccaa       900
     tccttctggg cagtcaagcc catcagatct acaggatgat gtgtgcaaaa ggctactcaa       960
     aaaaagactt ctcatccgtg ttccagtttc tacgagagga ggagacattc tgaacttgcc      1020
     ctttggctgg ggacactgtt ggaaaccaaa ctctcttgga gccacttata gcttacttca      1080
     caagtaaatg ggcttaatca gaggtcacct gtctgctttt gattgtctag gtcacaataa      1140
     accctgggac ttttcaccca tttttaactg cttattgttt ttatctattt agaaaacacc      1200
     tagtacctga attgtttttt ttttttcctt ttctgcaagc cactgatggt ctctgctagc      1260
     tagccaactg acctttttca aaagtttgat tcctgagcat cctcagctga aacgttgcct      1320
     acttcaatca ggatattatt tactccactt ttcactttca caaataagac caattttttt      1380
     caacaggata aaacctatcc tcaaggaagg agaagaaatt ggtatgagga aatgagttag      1440
     ggaaagggaa agtatagtga attactccct gtgtccctca ggaagtttat cctgttaacc      1500
     cagtggtggt ggtcttgcat tgaggttatg gtttattttc caagagttga taaagcaaga      1560
     gaatgccttg ctgcatgtta tgtaatttgg accctgttat agtatctcag tgtgcccctc      1620
     ttgtaacaac tcccaatact cagcaaactt tttttttaaa tatatttttg cttccttgta      1680
     cattcttact acatattttt tgacttcaga agaatgactt ctttagaact gtttcagagc      1740
     caatgatata tgctttagat aattattaca ttatcctaaa tataaccata ttattttgaa      1800
     ttcaaataaa tttctatgct gataataaaa aaaaaaaaaa aaaaaaaaaa aaa             1853
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