EBI Dbfetch

ID   BC090469; SV 1; linear; mRNA; STD; VRT; 2495 BP.
XX
AC   BC090469;
XX
DT   11-MAR-2005 (Rel. 83, Created)
DT   21-OCT-2008 (Rel. 97, Last updated, Version 8)
XX
DE   Danio rerio retinol saturase (all-trans-retinol 13,14-reductase), mRNA
DE   (cDNA clone MGC:113107 IMAGE:6900936), complete cds.
XX
KW   MGC.
XX
OS   Danio rerio (zebrafish)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Cyprinidae; Danio.
XX
RN   [1]
RP   1-2495
RX   DOI; 10.1073/pnas.242603899
RX   PUBMED; 12477932.
RG   Mammalian Gene Collection Program Team
RA   Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D.,
RA   Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F.,
RA   Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H.,
RA   Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K.,
RA   Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F.,
RA   Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S.,
RA   Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J.,
RA   Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J.,
RA   Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M.,
RA   Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X.,
RA   Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA   Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C.,
RA   Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I.,
RA   Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.;
RT   "Generation and initial analysis of more than 15,000 full-length human and
RT   mouse cDNA sequences";
RL   Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002).
XX
RN   [2]
RC   NIH-MGC Project URL: http://mgc.nci.nih.gov
RP   1-2495
RG   NIH MGC Project
RA   ;
RT   ;
RL   Submitted (22-FEB-2005) to the EMBL/GenBank/DDBJ databases.
RL   National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda,
RL   MD 20892-2590, USA
XX
DR   Ensembl-Gn; ENSDARG00000018600; Danio_rerio.
DR   Ensembl-Tr; ENSDART00000013167; Danio_rerio.
DR   ImaGenes; IMAGp958D233203Q.
DR   ImaGenes; IRALp962C1064Q.
DR   ImaGenes; IRBVp5006D017D.
XX
CC   Contact: MGC help desk
CC   Email: cgapbs-r@mail.nih.gov
CC   Tissue Procurement: Genome Institute of Singapore
CC   cDNA Library Preparation: S. Mathavan, Chia-Lin Wei, and Yijun
CC   Ruan, Genome Institute of Singapore
CC   cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
CC   DNA Sequencing by: National Institutes of Health Intramural
CC   Sequencing Center (NISC),
CC   Gaithersburg, Maryland;
CC   Web site: http://www.nisc.nih.gov/
CC   Contact: nisc_mgc@nhgri.nih.gov
CC   Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
CC   Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
CC   Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
CC   Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
CC   Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
CC   McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
CC   Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
CC   Young,A., Zhang,L.-H. and Green,E.D.
CC   Clone distribution: MGC clone distribution information can be found
CC   through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
CC   Series: IRAL Plate: 64 Row: c Column: 10
CC   This clone was selected for full length sequencing because it
CC   passed the following selection criteria: matched mRNA gi: 60688348.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2495
FT                   /organism="Danio rerio"
FT                   /lab_host="DH10B"
FT                   /mol_type="mRNA"
FT                   /clone_lib="GISZF001"
FT                   /clone="MGC:113107 IMAGE:6900936"
FT                   /tissue_type="Embryo, 7 different stages (from just
FT                   fertilized embryos to 72 hours just hatched baby fish)"
FT                   /note="Vector: pDNR-LIB"
FT                   /db_xref="taxon:7955"
FT   gene            1..2495
FT                   /gene="retsat"
FT                   /note="synonyms: fd46d06, zgc:113107, wu:fd46d06"
FT   CDS             44..1867
FT                   /codon_start=1
FT                   /gene="retsat"
FT                   /product="retinol saturase (all-trans-retinol
FT                   13,14-reductase)"
FT                   /db_xref="GOA:Q5BLE8"
FT                   /db_xref="InterPro:IPR002937"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q5BLE8"
FT                   /db_xref="ZFIN:ZDB-GENE-050320-11"
FT                   /protein_id="AAH90469.1"
FT                   /translation="MWFAVVAIFLALVAFLYRYVVGSGPNPFAIDTREPLKPMVFDRKL
FT                   KNKVLKQGFLASRVPEDLDAVVVGSGIGGLAIAVLLAKVGKKVLVLEQHDRAGGCCHTF
FT                   KEQGFEFDVGIHYIGELSNHKPLRCIIDQMTNGQLQWDPLENPFDNVVIGPPENRRIYQ
FT                   IYSGRKRYMDELKKCFPGEEKAIDEYVRLCKEVGQGVWVMVLLKFLPTPIANFLVRTGL
FT                   ANRLTSFSRYASRSLTDVVNELTQNKDLRAVLSYIFGTYGKIPKEASFSMHSLIVNHYM
FT                   NGAWYPKGGATEIAYHMIPIIEKAGGAVLVRAPVNRILLNDAKEAIGVSVLKGQEEVHV
FT                   RAPIVISDAGIFNTYEYLLPKDVQTMPAIQKQLSMLQHGDSGLSIFIGLDGTKEELGLK
FT                   ADNYFIYPENNIDELLEDYRSGNREESAKKNPLIFVASPSAKDSTWPERTPGKSTLTVV
FT                   SFANYEWFEEWKDDKVKNRSTDYKQLKELFINYILEAVTEIYPKIKDRIEYVDAGTPIT
FT                   NQHYIAAPRGEIYGADHGIPRFSAELNATIRAQTPIKNLYLTGQDLMLCGFAGALTGAL
FT                   TCGSVILNRNLHLEAFSLAKRVQNGNNKKKT"
XX
SQ   Sequence 2495 BP; 757 A; 488 C; 575 G; 675 T; 0 other;
     ggggactgac agcgcaagaa aatagcacgc tgcaacttac aaaatgtggt tcgctgtcgt        60
     cgcaattttc ttggctttag tggccttttt gtatagatat gttgtgggaa gtggaccgaa       120
     cccttttgcg atcgacacac gagagcccct gaagcccatg gtgttcgacc gaaagctgaa       180
     gaataaagtc ctgaaacaag gtttcttggc cagtagggtt cctgaggatt tggatgcagt       240
     agtggtcggc agtgggattg gtggactggc aattgctgtc ctgttggcca aagtgggcaa       300
     gaaagttctg gttctggagc aacatgaccg ggctggagga tgctgtcaca cctttaagga       360
     gcaaggcttt gagtttgatg ttggcatcca ctatattggg gaactgtcga atcacaagcc       420
     gttacgctgt attattgacc aaatgaccaa tgggcagctg cagtgggatc cgctggaaaa       480
     cccctttgac aacgtggtca ttggtccacc tgaaaaccgg cgcatatacc aaatctacag       540
     cggcagaaaa cgctacatgg acgaactaaa gaagtgcttc ccaggagagg aaaaggccat       600
     tgatgaatac gtgagacttt gcaaggaagt tggacaaggt gtatgggtta tggtcctgct       660
     gaaatttctc ccgaccccta ttgcaaattt cctggtgcgc acaggtctgg ccaatcgctt       720
     gacttccttc tctcgatatg cgtcccgcag cctaacggat gtggtgaatg agcttacaca       780
     gaataaggac ctgagagccg tgctctccta catctttgga acctatggta aaatacccaa       840
     agaagcaagc ttttccatgc acagtttgat tgtcaaccac tatatgaatg gtgcttggta       900
     cccaaagggt ggtgctactg agatcgccta ccacatgatc cccattattg agaaggcagg       960
     aggtgctgtg cttgttagag cacctgtaaa ccgcattctc ctcaatgatg ctaaagaggc      1020
     tattggtgtg agtgttctga aaggacagga ggaagtgcat gtacgagctc ccatagtgat      1080
     ttcagatgct ggaatcttta atacttatga atatctcctg cccaaagatg tgcagactat      1140
     gcctgcaatt cagaaacagt tgagtatgct gcagcatgga gacagtggtc tcagcatttt      1200
     cattggcctg gatgggacaa aagaggagtt gggcttaaaa gcagacaatt actttatcta      1260
     ccctgagaac aatatagacg agctgttgga agattatagg agcggaaaca gggaagaatc      1320
     tgctaaaaag aatcctctga tttttgtggc ctcaccctct gccaaagact caacctggcc      1380
     agaaagaaca ccaggtaaat ccaccctgac tgttgttagt tttgctaact atgaatggtt      1440
     tgaagaatgg aaagatgaca aagtgaagaa cagaagcaca gattataagc agttgaagga      1500
     gttgttcatc aattacatat tagaggcagt gactgagatc tatccgaaga tcaaggacag      1560
     gattgaatat gtggatgcag gcaccccaat cactaatcag cattacattg ccgcacctag      1620
     gggagagatt tatggcgctg atcatggaat tcctcgcttc agtgctgaac tgaatgccac      1680
     cattagagca cagacaccca ttaaaaacct ctaccttaca ggtcaggatc tgatgttgtg      1740
     tggttttgct ggagcgttga caggagctct gacttgcggt tctgtcatcc tgaaccggaa      1800
     tctacaccta gaagccttca gtcttgccaa aagagtccaa aatggcaaca acaagaagaa      1860
     aacataaaga tcagtattac ataatcttgg tagtcattca tatcacgtat ggctctcgag      1920
     aaggagaaca aagagagaac ctgcattatt tttaaagaaa tgaaccactg cctagtgcct      1980
     acagtaacaa tacactgcag agccttaccg gtataacaca caactctaat ctttcatcta      2040
     cttcttaaat caagggaaat acgtcagttg attcaataaa tggacttact gaaataatag      2100
     aattattttt gtaaataaaa aagtgttgat taaaaaattc cagttttctg aaatccaatt      2160
     aactttttta aatgtaattg aatattttta agcaagcaga agtaatgcac ttggtactgt      2220
     gtattttttg tttttaacta actgagcatg aactaaaatg gtgtcttttg attttaatta      2280
     ttgtggctct acagtgggtc atatgtctgt attcagggaa tttcatgcag atgtaagatg      2340
     aatgcaactt cacatttaca tgtgtgcttc ctttttaggt tgctgtgatt tttttcagac      2400
     ttgtacacta aatgtgctaa ttcaatggat gcttgtgcat accgaataaa cattttaact      2460
     tgaacaccaa aaaaaaaaaa aaaaaaaaaa aaaaa                                 2495
//