EBI Dbfetch

ID   BC075542; SV 1; linear; mRNA; STD; VRT; 1913 BP.
XX
AC   BC075542;
XX
DT   02-JUL-2004 (Rel. 80, Created)
DT   16-JUL-2006 (Rel. 88, Last updated, Version 8)
XX
DE   Xenopus tropicalis mannosyl (alpha-1,6-)-glycoprotein
DE   beta-1,2-N-acetylglucosaminyltransferase, mRNA (cDNA clone MGC:89475
DE   IMAGE:6991825), complete cds.
XX
KW   MGC.
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OS   Xenopus (Silurana) tropicalis (western clawed frog)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Mesobatrachia; Pipoidea; Pipidae; Xenopodinae; Xenopus;
OC   Silurana.
XX
RN   [1]
RP   1-1913
RX   DOI; 10.1002/dvdy.10174
RX   PUBMED; 12454917.
RA   Klein S.L., Strausberg R.L., Wagner L., Pontius J., Clifton S.W.,
RA   Richardson P.;
RT   "Genetic and genomic tools for Xenopus research: The NIH Xenopus
RT   initiative";
RL   Dev. Dyn. 225(4):384-391(2002).
XX
RN   [2]
RP   1-1913
RX   DOI; 10.1073/pnas.242603899
RX   PUBMED; 12477932.
RG   Mammalian Gene Collection Program Team
RA   Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D.,
RA   Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F.,
RA   Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H.,
RA   Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K.,
RA   Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F.,
RA   Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S.,
RA   Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J.,
RA   Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J.,
RA   Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M.,
RA   Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X.,
RA   Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA   Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C.,
RA   Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I.,
RA   Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.;
RT   "Generation and initial analysis of more than 15,000 full-length human and
RT   mouse cDNA sequences";
RL   Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002).
XX
RN   [3]
RC   NIH-MGC Project
RP   1-1913
RA   Klein S., Gerhard D.S.;
RT   ;
RL   Submitted (28-JUN-2004) to the EMBL/GenBank/DDBJ databases.
RL   National Institutes of Health, Xenopus Gene Collection (XGC), National
RL   Institute of Child Health and Human Development, 6100 Executive Boulevard,
RL   Room 4B01, Rockville, MD 20892-7510, USA
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DR   ImaGenes; IMAGp998K2414669Q.
DR   ImaGenes; IRAKp961J11170Q.
DR   ImaGenes; IRBNp992F0217D.
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CC   Contact: XGC help desk
CC   Email: cgapbs-r@mail.nih.gov
CC   Tissue Procurement: Robert M. Grainger
CC   cDNA Library Preparation: Life Technologies, Inc.
CC   cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
CC   DNA Sequencing by: Genome Sequence Centre,
CC   BC Cancer Agency, Vancouver, BC, Canada
CC   info@bcgsc.bc.ca
CC   Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
CC   Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
CC   Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
CC   Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
CC   Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
CC   Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
CC   Kim MacDonald,  Mike R. Mayo, Josh Moran, Diana Palmquist, JR
CC   Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
CC   Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
CC   Clone distribution: MGC clone distribution information can be found
CC   through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
CC   Series: IRAK Plate: 170 Row: j Column: 11
CC   This clone was selected for full length sequencing because it
CC   passed the following selection criteria: matched mRNA gi: 55742203.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1913
FT                   /organism="Xenopus (Silurana) tropicalis"
FT                   /lab_host="DH10B"
FT                   /mol_type="mRNA"
FT                   /clone_lib="NICHD_XGC_Emb6"
FT                   /clone="MGC:89475 IMAGE:6991825"
FT                   /tissue_type="Embryo, Silurana tropicalis, neurula (stages
FT                   14-19)"
FT                   /note="Vector: pCMV-SPORT6.1"
FT                   /db_xref="taxon:8364"
FT   gene            1..1913
FT                   /gene="mgat2"
FT                   /note="synonym: MGC89475"
FT   CDS             322..1620
FT                   /codon_start=1
FT                   /gene="mgat2"
FT                   /product="mannosyl (alpha-1,6-)-glycoprotein
FT                   beta-1,2-N-acetylglucosaminyltransferase"
FT                   /db_xref="GOA:Q6DIJ6"
FT                   /db_xref="InterPro:IPR007754"
FT                   /db_xref="UniProtKB/TrEMBL:Q6DIJ6"
FT                   /protein_id="AAH75542.1"
FT                   /translation="MRCRIYKRKVIILTLVVVACGLALWSSGRQKKNGFVPEVETDRFQ
FT                   SKGHISPAARKASNESLANKEQKAKVDNMTLAYRSVVFQWNFDQAIRNVEKINRPQDDV
FT                   VVVVQVHNRPEFLKRLLDSLGKAKGIENVLLVFSHDYWSPEINQIIASVDFCQVLQIFF
FT                   PFSIQLYPNEFPGHDPKDCPRDIKKKDAIELGCINAEYPDSFGHYREAKFSQTKHHWWW
FT                   KLQFVWDKLKVLKEHHGLVLFIEEDHYLAPDFYYILNKMWQKKNEECSDCDMLCLGTYA
FT                   QTPFADKAGKVEVKTWRSTEHNMGMAMTRETYKKLISCSETFCTYDDYNWDWTLQYLTV
FT                   NCLPKFWKVMVPEVPRIYHIGDCGMHHNKPCRPTTESAKLEALFTSSQQELFPENIAIS
FT                   RRYTMAALSPHVKNGGWGDIRDHELCKSYHRLQ"
XX
SQ   Sequence 1913 BP; 618 A; 332 C; 408 G; 555 T; 0 other;
     ccggcgttgg gattttcagt aagacaggag gaaaggagca ttaacagttt agcggaatct        60
     gtgctgctgg cacgaagggc atcctgcccc atgtcctttt aacaaatgct gcacggatcc       120
     tacactcatc tagtgacaag tgttttgcta atgatcttca ggggtgtact ctgcagatct       180
     ccattataga ccaggatgag ttagctcaaa gttatttttt ctttctaatg ctccagtctg       240
     gaatttgctt tccatcacac aggcaggccc tgtgttatgt gccagcttcc cttcgtttca       300
     tagctgcaaa gcacctataa catgaggtgt agaatataca aaaggaaagt gatcattctc       360
     acattggtgg tggttgcttg tggcttggct ttatggagta gtggaagaca aaagaaaaat       420
     ggttttgttc cagaagtgga aactgacaga tttcaaagca aggggcacat atcccctgca       480
     gctagaaaag cgtccaatga gtcactcgca aacaaagagc agaaggcaaa ggttgataac       540
     atgacactgg cctacagatc agtagtgttc caatggaatt ttgaccaagc tataagaaat       600
     gtagaaaaaa taaacaggcc tcaggatgat gtggttgtag tagtccaggt gcacaacagg       660
     cctgaatttt tgaaacgtct cttggactca cttggaaaag caaagggtat tgagaatgtg       720
     ctgttggtgt ttagccatga ttactggtcc ccagaaatta accagatcat tgctagtgtt       780
     gatttttgtc aagttttaca gatttttttc cccttcagta ttcagcttta tcccaacgaa       840
     tttcctggac atgatcctaa agactgtcca agagacataa agaaaaaaga tgcaattgaa       900
     ctgggctgta tcaatgcaga atatccagac tctttcggac attacagaga ggctaaattc       960
     tcccaaacaa aacatcactg gtggtggaaa ttgcagtttg tgtgggataa gttaaaggta      1020
     ttaaaagagc atcatggatt ggttttgttc attgaagaag atcactactt ggctccagat      1080
     ttttactaca tccttaacaa aatgtggcaa aagaaaaatg aagagtgctc tgactgtgac      1140
     atgctatgtc ttggaacata tgctcaaaca ccattcgctg acaaagctgg taaagtggaa      1200
     gtgaaaacgt ggagatccac tgagcataac atgggaatgg caatgaccag ggaaacttac      1260
     aaaaaactta tatcttgttc tgaaaccttc tgcacttatg atgactataa ctgggactgg      1320
     actcttcagt atttaactgt taattgtctt cctaagttct ggaaagttat ggtccctgag      1380
     gttcccagaa tttaccatat aggagactgt ggtatgcacc ataataagcc ttgtagaccc      1440
     actacagaaa gtgccaaact tgaggctttg tttacaagca gccaacaaga gttatttcct      1500
     gagaatatag ctattagcag gaggtataca atggctgctt tatcgcccca tgttaaaaat      1560
     ggtggatggg gtgatataag agaccatgaa ttgtgtaaaa gttatcatag gttacagtga      1620
     aactgaacct ggtgaacctt tttaagtttg taatattgca tactgtcttt gctgttccag      1680
     aaaactaagg ggatttatgg attttattaa gttgagcaat tgtacatgtc aaaattgtca      1740
     taatagacaa tgcatttcat ttgtgtgaaa tctcttaaga aaggctgttt ttgtccattg      1800
     tttcttacta atgaaaaaat caagcaatgt gcattatgtt tatcaaattc atttagaaaa      1860
     taaatgattt aattatctga aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa             1913
//