EBI Dbfetch

ID   BC075396; SV 1; linear; mRNA; STD; VRT; 1305 BP.
XX
AC   BC075396;
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DT   02-JUL-2004 (Rel. 80, Created)
DT   16-JUL-2006 (Rel. 88, Last updated, Version 9)
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DE   Xenopus tropicalis malate dehydrogenase 1, NAD (soluble), mRNA (cDNA clone
DE   MGC:89131 IMAGE:7007067), complete cds.
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KW   MGC.
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OS   Xenopus (Silurana) tropicalis (western clawed frog)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Mesobatrachia; Pipoidea; Pipidae; Xenopodinae; Xenopus;
OC   Silurana.
XX
RN   [1]
RP   1-1305
RX   DOI; 10.1002/dvdy.10174
RX   PUBMED; 12454917.
RA   Klein S.L., Strausberg R.L., Wagner L., Pontius J., Clifton S.W.,
RA   Richardson P.;
RT   "Genetic and genomic tools for Xenopus research: The NIH Xenopus
RT   initiative";
RL   Dev. Dyn. 225(4):384-391(2002).
XX
RN   [2]
RP   1-1305
RX   DOI; 10.1073/pnas.242603899
RX   PUBMED; 12477932.
RG   Mammalian Gene Collection Program Team
RA   Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D.,
RA   Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F.,
RA   Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H.,
RA   Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K.,
RA   Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F.,
RA   Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S.,
RA   Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J.,
RA   Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J.,
RA   Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M.,
RA   Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X.,
RA   Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA   Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C.,
RA   Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I.,
RA   Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.;
RT   "Generation and initial analysis of more than 15,000 full-length human and
RT   mouse cDNA sequences";
RL   Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002).
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RN   [3]
RC   NIH-MGC Project
RP   1-1305
RA   Klein S., Gerhard D.S.;
RT   ;
RL   Submitted (28-JUN-2004) to the EMBL/GenBank/DDBJ databases.
RL   National Institutes of Health, Xenopus Gene Collection (XGC), National
RL   Institute of Child Health and Human Development, 6100 Executive Boulevard,
RL   Room 4B01, Rockville, MD 20892-7510, USA
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DR   ImaGenes; IMAGp998K0114708Q.
DR   ImaGenes; IRAKp961L23171Q.
DR   ImaGenes; IRBNp992H0920D.
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CC   Contact: XGC help desk
CC   Email: cgapbs-r@mail.nih.gov
CC   Tissue Procurement: Rob Granger, University of Virginia
CC   cDNA Library Preparation: Open Biosystems
CC   cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
CC   DNA Sequencing by: Genome Sequence Centre,
CC   BC Cancer Agency, Vancouver, BC, Canada
CC   info@bcgsc.bc.ca
CC   Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
CC   Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
CC   Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
CC   Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
CC   Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
CC   Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
CC   Kim MacDonald,  Mike R. Mayo, Josh Moran, Diana Palmquist, JR
CC   Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
CC   Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
CC   Clone distribution: MGC clone distribution information can be found
CC   through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
CC   Series: IRAK Plate: 171 Row: l Column: 23
CC   This clone was selected for full length sequencing because it
CC   passed the following selection criteria: matched mRNA gi: 55742195.
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FH   Key             Location/Qualifiers
FH
FT   source          1..1305
FT                   /organism="Xenopus (Silurana) tropicalis"
FT                   /lab_host="DH10B"
FT                   /mol_type="mRNA"
FT                   /clone_lib="NICHD_XGC_Swb1N"
FT                   /clone="MGC:89131 IMAGE:7007067"
FT                   /tissue_type="Whole body, male, 10 months old, strain F6,
FT                   normal"
FT                   /note="Vector: pExpress1"
FT                   /db_xref="taxon:8364"
FT   gene            1..1305
FT                   /gene="mdh1"
FT                   /note="synonym: MGC89131"
FT   CDS             75..1079
FT                   /codon_start=1
FT                   /gene="mdh1"
FT                   /product="malate dehydrogenase 1, NAD (soluble)"
FT                   /db_xref="GOA:Q6DIY9"
FT                   /db_xref="InterPro:IPR016040"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q6DIY9"
FT                   /protein_id="AAH75396.1"
FT                   /translation="MPEPVKVLVTGAAGQIAYSLLYGIAKGDVFGKDQPLILVLLDITP
FT                   MMTVLEGVVMELQDCALPLLKEVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKA
FT                   NVKIFKSQGAALNKYSKKSVKVIVVGNPANTNCLTAMKSAPSIPKENFSCLTRLDHNRA
FT                   KGQIALKLNVASDDVKNVIIWGNHSSTQYPDASHATVNLQGKDVGAFEAVKNDDWLKGE
FT                   FITTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPDGQFVSMGVISDGNSYGIP
FT                   EDLMYSFPLTIKNKTWKIVEGLPINDFSREKMDLTAKELHEEKETALEFLSCE"
XX
SQ   Sequence 1305 BP; 392 A; 252 C; 303 G; 358 T; 0 other;
     gcagcagcta gctcagaagc agcgactcgc agcgcacgga aaggccattg ccatctgcac        60
     gtttcagaaa caaaatgcct gaacctgtga aagtccttgt gaccggagct gctggtcaga       120
     ttgcttactc cctgctctat ggtattgcta aaggcgatgt ctttggaaaa gaccagcctc       180
     ttatacttgt tcttttggat attaccccta tgatgactgt acttgaaggt gtagtgatgg       240
     agttgcagga ctgtgctctg cccctgctga aagaggtgat tgctaccgat aaggaagatg       300
     tggccttcaa agatttggat gttgccattc tggttggttc catgccaaga agggagggca       360
     tggaaagaaa ggacttgctg aaagccaatg taaagatttt taagtctcag ggtgcagctc       420
     tgaacaagta ttccaagaag agtgttaagg ttattgttgt ggggaatcca gcaaacacaa       480
     attgcctgac tgcaatgaag tctgctccat ccatccctaa agagaacttc agctgtctga       540
     cacgcttaga tcacaacaga gccaaaggac aaattgctct gaagttgaat gttgcttctg       600
     atgatgtaaa gaatgtaatt atctggggta atcactcatc cactcagtat cctgatgctt       660
     cccatgcaac tgtgaatctg caaggaaaag atgttggtgc ctttgaagca gtgaagaatg       720
     atgactggct aaagggtgaa tttatcacta cagtgcagca aagaggagca gctgttatta       780
     aggccagaaa gttgtctagt gcaatgtctg ctgctaaagc catctgcgat catgtgaggg       840
     atatctggtt tggaacccct gatgggcagt ttgtttccat gggagttatt tcggatggaa       900
     actcttatgg tatacctgaa gacctgatgt attcattccc tcttaccatc aagaataaga       960
     cctggaaaat agtggaaggc ctgcctatta atgacttttc ccgtgagaaa atggatctca      1020
     cagctaaaga acttcatgaa gaaaaagaaa ctgcacttga atttctgtcc tgcgaatgac      1080
     tagtgacaga ttccacaacg cacttaaccc ctgagaaaat gaaaagttct ctttaagttt      1140
     cctaccaagt gctatttatg ttgtctagca gtggtcacct gacagtcgca cttgattttg      1200
     ttttgtatat gtgactagtg ttatcaatca ttcctagcaa tagtcattaa atgaaaaccc      1260
     tgggtggttt taaagtgaaa aaaaaaaaaa aaaaaaaaaa aaaaa                      1305
//