 |
|
EBI DbfetchID BC067188; SV 1; linear; mRNA; STD; VRT; 2230 BP. XX AC BC067188; XX DT 18-MAR-2004 (Rel. 79, Created) DT 21-OCT-2008 (Rel. 97, Last updated, Version 3) XX DE Danio rerio lactate dehydrogenase A4, mRNA (cDNA clone MGC:76821 DE IMAGE:6960608), complete cds. XX KW MGC. XX OS Danio rerio (zebrafish) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; OC Cyprinidae; Danio. XX RN [1] RP 1-2230 RX DOI; 10.1073/pnas.242603899 RX PUBMED; 12477932. RG Mammalian Gene Collection Program Team RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., RA Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., RA Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., RA Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K., RA Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F., RA Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S., RA Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J., RA Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J., RA Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M., RA Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., RA Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., RA Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C., RA Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I., RA Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human and RT mouse cDNA sequences"; RL Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002). XX RN [2] RC NIH-MGC Project URL: http://mgc.nci.nih.gov RP 1-2230 RA Strausberg R.; RT ; RL Submitted (08-MAR-2004) to the EMBL/GenBank/DDBJ databases. RL National Institutes of Health, Mammalian Gene Collection (MGC), Cancer RL Genomics Office, National Cancer Institute, 31 Center Drive, Room 11A03, RL Bethesda, MD 20892-2590, USA XX DR Ensembl-Gn; ENSDARG00000040856; Danio_rerio. DR Ensembl-Tr; ENSDART00000059886; Danio_rerio. DR ImaGenes; IMAGp998G0714594Q. DR ImaGenes; IRAKp961P12145Q. DR ImaGenes; IRBOp991A0531D. XX CC Contact: MGC help desk CC Email: cgapbs-r@mail.nih.gov CC Tissue Procurement: Leonard I. Zon, M.D. CC cDNA Library Preparation: Invitrogen Corp CC cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) CC DNA Sequencing by: National Institutes of Health Intramural CC Sequencing Center (NISC), CC Gaithersburg, Maryland; CC Web site: http://www.nisc.nih.gov/ CC Contact: nisc_mgc@nhgri.nih.gov CC Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B., CC Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S., CC Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P., CC Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R., CC Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C., CC McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W., CC Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L., CC Young,A., Zhang,L.-H. and Green,E.D. CC Clone distribution: MGC clone distribution information can be found CC through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov CC Series: IRAK Plate: 145 Row: p Column: 12 CC This clone was selected for full length sequencing because it CC passed the following selection criteria: matched mRNA gi: 18858958. XX FH Key Location/Qualifiers FH FT source 1..2230 FT /organism="Danio rerio" FT /lab_host="DH10B" FT /mol_type="mRNA" FT /clone_lib="NCI_CGAP_ZKid1" FT /clone="MGC:76821 IMAGE:6960608" FT /tissue_type="Kidney, zebrafish" FT /note="Vector: pCMV-SPORT6.1" FT /db_xref="taxon:7955" FT gene 1..2230 FT /gene="ldha" FT CDS 80..1081 FT /codon_start=1 FT /gene="ldha" FT /product="ldha protein" FT /db_xref="GOA:Q9PVK5" FT /db_xref="HSSP:1I10" FT /db_xref="InterPro:IPR015955" FT /db_xref="UniProtKB/Swiss-Prot:Q9PVK5" FT /db_xref="ZFIN:ZDB-GENE-991026-5" FT /protein_id="AAH67188.1" FT /translation="MASTKEKLIAHVSKEQPAGPTNKVTVVGVGMVGMAAAVSILLKDL FT TDELALVDVMEDKLKGEAMDLQHGSLFLKTHKIVADKDYSVTANSKVVVVTAGARQQEG FT ESRLNLVQRNVNIFKFIIPNIIKYSPNCILLVVSNPVDILTYVAWKLSGLPRNRVIGSG FT TNLDSARFRYLMGEKLGIHPSSCHGWVVGEHGDSSVPVWSGVNVAGVSLQALNPDLGTD FT KDKEDWKSVHKMVVDSAYEVIKLKGYTSWAIGMSVADLCESILKNMHKCHPVSTLVKGM FT HGVNEEVFLSVPCILGNNGLTDVVHMTLKPEEEKQLVKSAETLWGVQKELTL" FT misc_feature 194..565 FT /gene="ldha" FT /note="Ldh_1_N; Region: lactate/malate dehydrogenase, NAD FT binding domain. L-lactate dehydrogenases are metabolic FT enzymes which catalyse the conversion of L-lactate to FT pyruvate, the last step in anaerobic glycolysis. FT L-2-hydroxyisocaproate dehydrogenases are also members of FT the family. Malate dehydrogenases catalyse the FT interconversion of malate to oxaloacetate. The enzyme FT participates in the citric acid cycle. L-lactate FT dehydrogenase is also found as a lens crystallin in bird FT and crocodile eyes. N-terminus (this family) is a Rossman FT NAD-binding fold. C-terminus is an unusual alpha+beta fold" FT misc_feature 569..1072 FT /gene="ldha" FT /note="Ldh_1_C; Region: lactate/malate dehydrogenase, FT alpha/beta C-terminal domain. L-lactate dehydrogenases are FT metabolic enzymes which catalyse the conversion of FT L-lactate to pyruvate, the last step in anaerobic FT glycolysis. L-2-hydroxyisocaproate dehydrogenases are also FT members of the family. Malate dehydrogenases catalyse the FT interconversion of malate to oxaloacetate. The enzyme FT participates in the citric acid cycle. L-lactate FT dehydrogenase is also found as a lens crystallin in bird FT and crocodile eyes" XX SQ Sequence 2230 BP; 729 A; 420 C; 467 G; 614 T; 0 other; aaaaaaagtc acagctgaga cattccagcc catcctactc tgaattacac agtcaaatat 60 caactatcca cagttgaaga tggcctcaac aaaggagaaa ctcattgctc acgtgagcaa 120 ggagcagcct gctgggccca caaacaaggt gacagtggtt ggtgttggaa tggttggaat 180 ggctgccgcc gtcagcatcc ttctcaagga tctgaccgat gaactcgccc tggtggacgt 240 gatggaagat aagctgaaag gagaggctat ggacttgcag catggaagcc tgtttctcaa 300 gacgcacaag attgtagcgg ataaagatta cagtgtgacc gcaaactcaa aagtggttgt 360 tgtgaccgct ggagctcgtc agcaggaggg tgagagccgc ctgaacctcg tccagaggaa 420 tgttaacatc ttcaagttca ttatcccaaa catcatcaag tacagcccca actgcatcct 480 tttggtggtc tcaaatcctg ttgacatctt gacctatgtg gcctggaagc tgagcggttt 540 gcccaggaac cgtgtgattg gcagtggcac aaacttggat tctgctcgtt tccgctactt 600 gatgggagag aagttgggaa ttcatccatc tagctgccat ggttgggtcg ttggagaaca 660 tggagactcc agcgttcctg tgtggagtgg tgtgaatgtg gctggggtgt ccctccaagc 720 tctaaaccct gacttgggta cagataaaga caaggaggac tggaagagcg tccacaagat 780 ggtggttgac agtgcatatg aagttattaa gctgaagggt tacacttctt gggctattgg 840 aatgtctgta gctgacctgt gtgaaagtat cttgaagaac atgcacaaat gtcacccagt 900 ttctactttg gtcaagggaa tgcatggtgt aaatgaagag gttttcctaa gtgtgccttg 960 cattctgggt aataatggcc tgacagatgt cgtccacatg accctcaaac cagaagagga 1020 aaaacagttg gtgaagagcg ccgagacctt gtggggtgtt cagaaggagc tgaccctgtg 1080 aaccgcggtc tcctccaaaa tgtagccaca aattgcaaac ctcaatgagt cctgcctatc 1140 tgtctcctca cctcataaac ctttaatgcc tgttaagatt agataactcc atgtcactcg 1200 cagaccatta gataacactc cttgtgttag tgattttaac ttcaaaggga taatttattc 1260 ctgttgtctc tacaaaacaa tcagccttag tgaaagtgtg tgtgtctata tgcatattat 1320 tgtctattca agaaagaagc acttgtaaga tgtacaattg cagactctta ttgtattccg 1380 ttctgaggtc agctagcatt ccgaacttgc ctcctgcatc aagcaattgg tcatttacca 1440 acaagcaaac ttcattgtaa tatcaattct atgttttgca tgtacaacaa aatagctctt 1500 tcgctttaaa tctacaatta ttatttataa aaatttgata tcgacctaaa aaaaagaatg 1560 gaataatcct tatttatgaa aaatgtatct gttgattgat taatacttct atttattaaa 1620 tgcaacaatg tatcttacaa tgggcctata aataatttgt aatttctaaa ttaaatggtt 1680 catattgttt taaaaaggtt aaagttaaac aaagtgactt tataaaccat cctgaattta 1740 cagttgtaat ggtttaatga atggacaaat aagtttccaa catgagtgaa gtccattgac 1800 caccatagca gagcctctga acaaaaaaaa atttttattt atgaactgct taaggtattt 1860 gaatagatgt tgacaaatac atgctgagag tgcatgtaac caaactcaat aagggaaata 1920 cagtagtgca aatgatggta ataactatat caccccaact actcgtgtta agtccacact 1980 atatgtatca tctgaaaaaa tgctgttgtc agtgtatttt ttgtgtgtgt ttatcagaac 2040 aacaaagcgc tcctatccag gtggtaaaac acagcagtgg ttttagcttt tacttgaaat 2100 gtgtgattgt agaattaaga cacagcccga ctgaataaac attgtgacta tctacaaaaa 2160 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 2220 aaaaaaaaaa 2230 // |
|