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EBI DbfetchID BC064683; SV 1; linear; mRNA; STD; VRT; 3406 BP. XX AC BC064683; XX DT 24-DEC-2003 (Rel. 78, Created) DT 19-MAR-2009 (Rel. 100, Last updated, Version 5) XX DE Xenopus laevis probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, DE mitochondrial precursor, mRNA (cDNA clone MGC:68840 IMAGE:4679813), DE complete cds. XX KW MGC. XX OS Xenopus laevis (African clawed frog) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia; OC Batrachia; Anura; Mesobatrachia; Pipoidea; Pipidae; Xenopodinae; Xenopus; OC Xenopus. XX RN [1] RP 1-3406 RX DOI; 10.1002/dvdy.10174 RX PUBMED; 12454917. RA Klein S.L., Strausberg R.L., Wagner L., Pontius J., Clifton S.W., RA Richardson P.; RT "Genetic and genomic tools for Xenopus research: The NIH Xenopus RT initiative"; RL Dev. Dyn. 225(4):384-391(2002). XX RN [2] RP 1-3406 RX DOI; 10.1073/pnas.242603899 RX PUBMED; 12477932. RG Mammalian Gene Collection Program Team RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., RA Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., RA Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., RA Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K., RA Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F., RA Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S., RA Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J., RA Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J., RA Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M., RA Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., RA Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., RA Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C., RA Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I., RA Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human and RT mouse cDNA sequences"; RL Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002). XX RN [3] RC NIH-MGC Project RP 1-3406 RA Klein S., Gerhard D.S.; RT ; RL Submitted (22-DEC-2003) to the EMBL/GenBank/DDBJ databases. RL National Institutes of Health, Xenopus Gene Collection (XGC), National RL Institute of Child Health and Human Development, 6100 Executive Boulevard, RL Room 4B01, Rockville, MD 20892-7510, USA XX DR ImaGenes; IMAGp998F0610557Q. DR ImaGenes; IRAKp961A12129Q. DR ImaGenes; IRBHp990F0718D. XX CC Contact: XGC help desk CC Email: cgapbs-r@mail.nih.gov CC Tissue Procurement: Dr. Igor Dawid CC cDNA Library Preparation: Life Technologies, Inc. CC cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) CC DNA Sequencing by: Sequencing Group at the Stanford Human Genome CC Center, Stanford University School of Medicine, Stanford, CA 94305 CC Web site: http://www-shgc.stanford.edu CC Contact: (Dickson, Mark) mcd@paxil.stanford.edu CC Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers, CC R. M. CC Clone distribution: MGC clone distribution information can be found CC through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov CC Series: IRAK Plate: 129 Row: a Column: 12 CC This clone was selected for full length sequencing because it CC passed the following selection criteria: matched mRNA gi: 40352989 CC Unstable clone, instability not involving the open reading frame. XX FH Key Location/Qualifiers FH FT source 1..3406 FT /organism="Xenopus laevis" FT /lab_host="DH10B" FT /mol_type="mRNA" FT /clone_lib="NICHD_XGC_Emb4" FT /clone="MGC:68840 IMAGE:4679813" FT /tissue_type="Embryo, stage 31/32, Xenopus" FT /note="Vector: pCMV-SPORT6" FT /db_xref="taxon:8355" FT gene 1..3406 FT /gene="MGC68840" FT /gene_synonym="dhtkd1" FT CDS 144..2927 FT /codon_start=1 FT /gene="MGC68840" FT /gene_synonym="dhtkd1" FT /product="MGC68840 protein" FT /db_xref="GOA:Q6P286" FT /db_xref="InterPro:IPR005475" FT /db_xref="UniProtKB/Swiss-Prot:Q6P286" FT /protein_id="AAH64683.1" FT /translation="MNVFAHRCSSCRRAVVLWRPGLHRLYRTERGVYGYRPKISAGSQL FT QESSTRGKQAASPTVDHGLARLVTAYREHGHKAAKINPLFTGQAVMDMVPEIQEIMEIL FT HGPFSTTGLLNMGKSEATIEEVLAYLDHTYCGQISIETSQLQNYKEREWFSRRFEELKQ FT ESFSTEERKHLARLMLECQEFDHFLATKFSTVKRYGGEGAESMMGFFHEMLKMCSFGGV FT TDVIIGMPHRGRLNLLTGLLQFPPELMFRKMRGLSEFPENSPSIGDVLSHLTSSVDLDF FT GSHRPLHVTMLPNPSHLEAINPVAVGKTRARQQSLSDGDYSTESSAQPGDKVVCLQVHG FT DASISGQGIVTETFTLSNLPHYRIGGSIHLIVNNQLGYTTPAERGRSSLYSSDVGKIVG FT CAVIHVNGDDPEEVLRATRLAVEYQRCFRKDVIIDLLCYRQWGHNELDEPFFTNPSMYK FT IIRSRKSIPDVYSERLIAEGLMTEEEATEIRTTYYSKFNDHLSNMTLYSPPSTNLQAHW FT REMIEPSARTTTWDTGLPADLLKFIGAKSVEVPEEFKMHSHLLKMHAQSRVQKLQEATK FT LDWATAEALAFGSLLCQGFNIRISGQDVGRGTFSQRHAMLVCQETNDTYIPLNHMTPDQ FT KGFLEVSNSALSEEAVLGFEYGMSIESPKLLPIWEAQFGDFFNGAQIIFDTFISGGEAK FT WLLQSGIVILLPHGYDGAGPEHSSCRIERFLQMCDSTEEGVDGDTVNMFVVHPTTPAQY FT FHLLRRQMVRSFRKPLIVASPKMLLRYPAAVSSLEDIAPGKTFRSVIGDSSADPKSVSK FT VILCSGKHYYALHKQREALGEQGRSSAIIRVEELCPFPLEALQQEIHRYPKAKDFIWSQ FT EEPQNMGAWTFVAPRFEKQLACKLRLVSRPALPAPAVGIGTLHQQQQEEITVKTLS" XX SQ Sequence 3406 BP; 1049 A; 645 C; 752 G; 960 T; 0 other; cgagggaatg ctgtcatttt ctgacagctt tgtaaggcta ttacgttgta gtgcaatggt 60 agcagtagca tgtgatattt tcttcatacc acgcggacct ggatattttt ggagtttgca 120 aaagaattta ctgactgtca tggatgaatg tgtttgctca tcgctgcagc tcatgcaggc 180 gggcggttgt attatggaga cccggcctgc acagattgta tcgcacggag cgtggggtgt 240 acgggtacag accgaagata agcgcaggca gccagctcca ggagagcagt acccgaggca 300 agcaagcggc cagcccgaca gtggatcatg gccttgctcg ccttgttact gcttaccgtg 360 aacatggtca caaagcagcc aagattaacc cattatttac tggtcaggca gtaatggata 420 tggtaccaga gatacaagag ataatggaaa tactccatgg accatttagc actactggac 480 tattgaacat gggaaaatca gaagcaacaa ttgaagaggt gttagcatat ctggatcata 540 catattgtgg ccagatttct attgaaacaa gccagttaca aaattacaaa gagagggaat 600 ggttttccag aagatttgaa gagttgaaac aagaaagttt ttcaacagaa gagaggaaac 660 atttagcaag actgatgtta gaatgtcagg aatttgacca ctttctggcc actaagtttt 720 caacggtgaa gcggtatgga ggagagggcg ctgagagcat gatgggattt ttccatgaga 780 tgctaaaaat gtgttcattt ggtggtgtta cagatgtaat tataggaatg ccccacagag 840 ggaggctaaa tcttcttaca ggacttctcc agtttccacc agagctgatg tttcgtaaaa 900 tgcgtggctt aagcgagttc cctgaaaact ccccttcaat aggcgacgta ctttcccact 960 taacttcttc tgtagatctg gattttggtt cccaccgacc tctgcatgta actatgttac 1020 caaatccttc acacttggaa gcaataaacc cagtagctgt tggcaaaact cgagcaaggc 1080 agcaatcctt gtcagatggt gattattcta cagagagctc tgcacaacct ggtgacaagg 1140 ttgtgtgtct acaggtacat ggtgatgctt caatttctgg tcaaggaatt gttacagaaa 1200 cttttacatt atcaaatctt cctcattatc ggataggcgg aagcattcat ctgattgtta 1260 ataaccagtt aggttataca acgcctgctg aacgtgggag gtcttccttg tacagcagcg 1320 atgttggcaa aattgttggc tgtgctgtaa tacatgttaa cggagatgac cctgaagagg 1380 ttttaagagc tacacgatta gcagtcgaat atcaaaggtg ttttcgcaag gatgtaatca 1440 tagatttact ctgctacaga caatggggac ataatgaact tgatgagcca tttttcacaa 1500 atcctagcat gtataaaatt atccgatcta gaaaaagcat acctgatgtt tactctgagc 1560 gattaattgc ggaagggcta atgacagagg aagaagcaac tgagataagg actacttact 1620 actccaaatt taatgaccac ctttcgaata tgacattata cagtccacca tccacaaact 1680 tgcaggctca ttggagggag atgattgaac catctgctcg aaccacaaca tgggatacag 1740 gtcttcctgc tgatcttctc aagtttattg gagccaagtc tgtagaagtt cctgaggaat 1800 tcaaaatgca cagccatctt ctcaaaatgc atgcacagtc tagagtacaa aagctgcagg 1860 aagcaaccaa gctagactgg gccacggctg aagctttggc ttttgggtca ttgctttgcc 1920 aaggctttaa tattcgaatt agtggtcagg atgtgggaag aggaaccttc agtcagaggc 1980 atgcaatgct ggtctgtcag gaaactaatg acacatacat ccctttgaac cacatgactc 2040 ctgatcaaaa gggatttttg gaggtgagca acagtgcatt gtccgaagaa gctgtgcttg 2100 gatttgaata tggaatgagc attgaaagcc ccaaattgct tccaatttgg gaggcacagt 2160 ttggtgattt tttcaatggt gcacaaataa tatttgacac tttcatttct ggaggtgaag 2220 ctaaatggct tttacaaagt ggtattgtaa ttctccttcc ccatggctac gatggtgctg 2280 gcccagaaca ctcctcatgc agaattgaac gctttttaca gatgtgtgac agtacagagg 2340 aaggagttga tggagatacc gttaatatgt ttgtagtgca tccaactact cctgcacagt 2400 actttcacct gctgcgcaga cagatggttc gcagtttcag gaaacctctc attgttgctt 2460 cacctaaaat gcttctaaga tatccggctg ctgtttcaag ccttgaggat attgcaccag 2520 gaaaaacatt tagatctgtt attggagatt catctgcaga ccccaaaagt gtaagcaaag 2580 tcatactttg ctctggcaaa cattactatg cactgcacaa acagagagaa gccctaggag 2640 agcaagggcg cagctctgct atcatcagag tggaagagct ttgcccattt cctttagaag 2700 ctctgcaaca agaaattcat agatacccaa aggccaaaga ttttatttgg agccaagaag 2760 agccacagaa catgggtgcc tggacatttg ttgcacccag gtttgaaaaa caacttgctt 2820 gtaagttgcg tcttgtaagt agacctgctc ttccagcacc agctgtaggt attggcaccc 2880 tgcatcaaca gcaacaagaa gaaatcacgg tgaaaacatt atcgtaactg gaataatagt 2940 gaactttatt tttttctaca tttggactct ttggcttgtg atgaatgtga ttttgcagtt 3000 ttatggagtt tttagctatt ggctcattga gcagacaaaa aaattatgtt gtttgtactc 3060 agaaatcctg ccatcattcc tcttataata gggatcaaag cactacagaa aagctagtgt 3120 ttgtcagcag tgtgtatata tatatatata tatatatata tatatatata tatatatata 3180 tatatatata tatatatata tatatatata tatatatata tatctctaca acattttata 3240 actgcttgaa atgcacagag taagaatgat atcaggaaga aaatatttac tgtaaacata 3300 tgaatattgc tgtgatcaga catttgaaac catttatgtc tttggcaaca taaaccatta 3360 aaatgattca gtagtgtaaa aaaaaaaaaa aaaaaaaaaa aaaaaa 3406 // |
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