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EBI DbfetchID BC064670; SV 1; linear; mRNA; STD; VRT; 3982 BP. XX AC BC064670; XX DT 24-DEC-2003 (Rel. 78, Created) DT 16-JUL-2006 (Rel. 88, Last updated, Version 9) XX DE Danio rerio carbohydrate (chondroitin) synthase 1, mRNA (cDNA clone DE MGC:64191 IMAGE:6798417), complete cds. XX KW MGC. XX OS Danio rerio (zebrafish) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; OC Cyprinidae; Danio. XX RN [1] RP 1-3982 RX DOI; 10.1073/pnas.242603899 RX PUBMED; 12477932. RG Mammalian Gene Collection Program Team RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., RA Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., RA Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., RA Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K., RA Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F., RA Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S., RA Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J., RA Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J., RA Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M., RA Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., RA Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., RA Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C., RA Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I., RA Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human and RT mouse cDNA sequences"; RL Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002). XX RN [2] RC NIH-MGC Project URL: http://mgc.nci.nih.gov RP 1-3982 RG NIH MGC Project RA ; RT ; RL Submitted (22-DEC-2003) to the EMBL/GenBank/DDBJ databases. RL National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda, RL MD 20892-2590, USA XX DR Ensembl-Gn; ENSDARG00000079027; Danio_rerio. DR Ensembl-Tr; ENSDART00000104536; Danio_rerio. DR ImaGenes; IMAGp998E0814313Q. DR ImaGenes; IRAKp961O09117Q. DR ImaGenes; IRBOp991G108D. XX CC Contact: MGC help desk CC Email: cgapbs-r@mail.nih.gov CC Tissue Procurement: Dr. Chi-Bin Chien CC cDNA Library Preparation: Invitrogen Corp CC cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) CC DNA Sequencing by: Sequencing Group at the Stanford Human Genome CC Center, Stanford University School of Medicine, Stanford, CA 94305 CC Web site: http://www-shgc.stanford.edu CC Contact: (Dickson, Mark) mcd@paxil.stanford.edu CC Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers, CC R. M. CC Clone distribution: MGC clone distribution information can be found CC through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov CC Series: IRAK Plate: 117 Row: o Column: 9 CC This clone was selected for full length sequencing because it CC passed the following selection criteria: matched mRNA gi: 40352709. XX FH Key Location/Qualifiers FH FT source 1..3982 FT /organism="Danio rerio" FT /lab_host="DH10B" FT /mol_type="mRNA" FT /clone_lib="NCI_CGAP_ZEmb3" FT /clone="MGC:64191 IMAGE:6798417" FT /tissue_type="Embryo, zebrafish whole embryo 72 hpf" FT /note="Vector: pCMV-SPORT6.1" FT /db_xref="taxon:7955" FT gene 1..3982 FT /gene="chys1" FT /note="synonyms: Chsy1, fc27h05, zgc:64191, fc27h06, FT wu:fc27h06, wu:fc27h05" FT CDS 286..2691 FT /codon_start=1 FT /gene="chys1" FT /product="carbohydrate (chondroitin) synthase 1" FT /db_xref="GOA:Q6P296" FT /db_xref="InterPro:IPR008428" FT /db_xref="UniProtKB/TrEMBL:Q6P296" FT /db_xref="ZFIN:ZDB-GENE-030131-3127" FT /protein_id="AAH64670.1" FT /translation="MAGRSRRSWFSVLLGLVVGFTLASRLILPRASELKKAGQKRRASG FT GGAGGCGGGGGMMMMMKKEYGGVLMPGTEKPSVPQSSSFLFVGVMTAQKYLNNRAVAAY FT RTWAKTIPGKVEFFSSEGSDTTIPIPIVALQNVDDSYPPQKKSFMMLKYMHDHYLDQFE FT WFMRADDDVYIKSEKLESFLRSLNSSEAIFLGQTGMGARDELGKLALEPGENFCMGGPG FT VIMSREVLRRMVPHIRQCLQEMYTTHEDVEVGRCVRRFAGVQCVWSYEMQQLFYENYEP FT NKKGYIRDLHNSKIHRAITLHPNKNPPYQYRLHSYMLSRKIAELRHRTIQLHREIVQMS FT RYSNTEVHREDLQLGMPPSFMRFHPHQREEVLEWEFLTGKYIFSSSDGQPPRRGIDSSQ FT KMALDDIIMQVMEMINANAKTRGRVIDFKEIQYGYRRVNPMYGAEYVLDLLLLYKKHKG FT KTMTVPVRRHAYLQQTFSKIQFLEEEEMDARVLAARINQDSDSLSFLSNSLKMLVPFKL FT SSPGIEQHEPKEKKINILVPLAGRYEIFLRFMANFEKICLIPNQNVKLLILLFSTDNNT FT ERIKQIELMREYRMKYPKADMEIKPVSGPFSRALALEVGSAHFTNDSLLFYCDVDLLFT FT PDFLTRCRGNTILGEQTYFPIIFSQYDPKVVYAGKVPSDNHYVFTSKTGLWRHYGFGIV FT CVYKGDLVKAGGFDVSIQGWGLEDVDLFNKFVQSGIKLFRSTDTGIVHVHHPVVCDPNL FT DPKQYKMCLGSKASSHGSTQQLAELWLEKNNHSFRKISNSNNESMRTE" XX SQ Sequence 3982 BP; 1044 A; 939 C; 988 G; 1011 T; 0 other; cgagaatgag gatgcagccg cacttctttc cgcaacaaac cgttaattgc tcctgactac 60 ccaccgactc gggctcgaaa tcacggagat acctttacga accccgtcat ggctcttccg 120 gagcggatag tgtgtgatta gatggcagcg gttggtgatt tatggggacc ggagcggtga 180 ccatgctcgg acccaaaccc agcgttgtgg acgggactgc agcagcagca acgggcggcg 240 tttaataaac aaaactctga caaattaaag cgactagtag ctgaaatggc aggaaggagt 300 cgcagatctt ggtttagcgt gttgctgggg ctggtggtcg ggttcacact ggcgtccagg 360 ctcatcctgc cccgggccag cgagctgaag aaagcgggtc agaagcggcg ggcgagcggc 420 ggcggcgcgg gaggctgcgg aggcggcgga gggatgatga tgatgatgaa gaaggagtac 480 ggaggggtct tgatgcccgg taccgagaag ccctcagtgc cccagtctag cagctttctg 540 tttgtgggcg tcatgaccgc gcagaagtac ctgaataacc gcgccgtggc agcatatagg 600 acctgggcca agaccatccc gggcaaggtg gagtttttct ccagtgaagg ttctgacacc 660 accatcccca tccccattgt ggctctgcag aacgtggatg actcgtaccc tccgcagaag 720 aagtccttca tgatgctcaa atacatgcat gaccactatt tggaccagtt cgagtggttc 780 atgcgagccg acgacgacgt ctacatcaaa agcgaaaagc tggagagttt cctaaggagt 840 ctcaacagca gcgaggccat ctttctgggc cagacgggca tgggagcccg cgacgaactg 900 ggcaaactgg cgctggagcc gggggagaac ttctgcatgg gcggccctgg tgttatcatg 960 agccgcgagg tgctgcgcag gatggttccc cacatccgcc agtgcctgca ggagatgtac 1020 accacccatg aggacgtgga ggtgggccgc tgcgtgcgca gattcgccgg agtgcagtgt 1080 gtttggtcct acgagatgca gcagctgttc tatgagaatt acgagccaaa caagaagggc 1140 tacatccgtg atctccacaa cagtaagatc cacagagcca taacccttca ccccaacaag 1200 aacccacctt accagtatcg gttgcacagc tacatgttga gccggaagat tgcagagctg 1260 cgccaccgca ccattcagct ccatcgtgag attgtgcaaa tgagccgcta cagcaacacc 1320 gaggtgcaca gggaagacct tcaactgggc atgcctccat ccttcatgcg ctttcatccc 1380 caccaacgtg aggaggtgct ggaatgggaa ttcctcacgg gaaagtacat tttctcatcc 1440 tcagatggac agcctccccg tcgaggcatt gactcctcgc agaagatggc gctggacgac 1500 atcatcatgc aagttatgga gatgattaac gccaatgcca agacacgcgg aagagtcatt 1560 gacttcaaag agatccaata tggctaccga agggtcaacc ctatgtatgg tgcagagtac 1620 gtactggatc tgctgctgct ctacaagaag cacaagggga agactatgac agtgcccgta 1680 aggaggcatg cgtatcttca gcagaccttc agcaagatcc agttcttgga agaagaagag 1740 atggacgctc gagtcctggc tgccaggatc aaccaagact ccgactcgct gtctttcttg 1800 tccaactccc tcaagatgct cgtcccgttc aagttgagca gcccaggcat cgaacaacac 1860 gaacccaaag agaagaagat caacatcctt gtgccgctgg ccggacgcta cgagattttt 1920 ctgcgcttca tggccaactt cgagaagatc tgcctcattc ccaaccagaa tgtcaagctt 1980 ctgattctgc ttttcagcac ggacaacaac actgagcgca tcaagcaaat cgagctcatg 2040 agggagtatc gcatgaagta ccccaaagcc gatatggaga tcaaaccggt ttccggacca 2100 ttctcccgtg ctctggccct ggaggtcggc tcggcccact ttaccaatga ttctttactc 2160 ttctactgtg acgtggactt actgtttact cctgacttct tgactcgatg tcgtgggaat 2220 accatacttg gggaacagac atactttcct attattttca gccagtatga cccaaaggtt 2280 gtctacgcag ggaaggtgcc cagcgacaac cactacgtgt tcacatccaa aacaggcctg 2340 tggagacact atggttttgg tattgtctgc gtctacaaag gagacctggt caaagctggt 2400 ggttttgacg tctccatcca aggttgggga ctggaggacg ttgacctttt caacaagttt 2460 gtccaatccg ggatcaagct gttccgcagt acggataccg gcatcgttca cgtgcatcac 2520 cctgtggttt gcgaccccaa tcttgatccc aagcagtaca aaatgtgcct gggatccaaa 2580 gcatcctcgc acggctccac gcagcaactc gcagagctat ggttggagaa aaacaaccac 2640 agcttccgaa agatctcgaa ctccaataat gaatcaatga ggacagaata gacctggcaa 2700 gtgcacattg tccatccgtc ttcctcttaa tggtgttttt taaaactacc ttatttattt 2760 tttacaggaa aaagacttct gtggatatat tttgaaataa taactggttt tgtaaactgt 2820 acaggagctg gccgtcacct tcagacttga tcttttttta agtagcgttt caaataacac 2880 aacagtgact taaagaggac gatctatttt acaaatccaa tactgagcgt tatatttatg 2940 gcacctcaaa aacagactgc tgtggacctc gccagaaaaa aaatgctttg aaatggagtc 3000 gaagagggag tttggacacg gacttgtgtg gtgtctggca agatggtaaa tgtttatttg 3060 tgaatgttta cagagagcga tgaacaagtg attcattctt ggtgaagggg atctatagaa 3120 taacaatctt aattctgaca attaagggcc attgatcaac agcttctttt cggttcgttt 3180 tttccagttt acttgtctgg agttgggagg gggatccata ataacactgt ttcgttttta 3240 tcgcttttga tttattgttt ttattgactt ctgtgaatga aactgctggc tgttgatctc 3300 tcctactcat tcccactgtc taatttattg agcgttcagt cttcggggct gaggttgtgt 3360 atattagaga ttgtataacc gtttcagtca tggatagtgt ttttgttccc tctctcgatc 3420 ttgaattact ttaattacca aagactttct ttcttttttt tttttttttg cacagttgct 3480 gtaggtgata tttgttcaag agtgcttgtt tttgtgtgtg cgtgcgtgta tgagtccaaa 3540 tgcaaggcag tagagcagga aaaaacatgt aatgtactgt aggatggagg taccgatgac 3600 tattcaacat ggccctatga aagtatgccg acaacaagcc acttaaagag attttctcat 3660 ttcatatgat cagatttcat ttactcctct tggttattta aattttttga ttcagttggc 3720 ctttctcctg tgattgagcc atgcaccctg tggggacaac ggccgtaacg cggccccgcc 3780 ttcagattcg aggacgatct cccacagtag tctctctttc tgttgtcttt tgtttttctc 3840 tggggcgttt tcatacttca gttctttgtt agcccccata caactgggac aaagttttat 3900 atttaataaa atggaaaatg aaaaagaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 3960 aaaaaaaaaa aaaaaaaaaa aa 3982 // |
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