 |
|
EBI DbfetchID BC060386; SV 1; linear; mRNA; STD; VRT; 1320 BP. XX AC BC060386; XX DT 30-OCT-2003 (Rel. 77, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Xenopus laevis hypothetical protein MGC68659, mRNA (cDNA clone MGC:68659 DE IMAGE:4032761), complete cds. XX KW MGC. XX OS Xenopus laevis (African clawed frog) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia; OC Batrachia; Anura; Mesobatrachia; Pipoidea; Pipidae; Xenopodinae; Xenopus; OC Xenopus. XX RN [1] RP 1-1320 RX DOI; 10.1002/dvdy.10174 RX PUBMED; 12454917. RA Klein S.L., Strausberg R.L., Wagner L., Pontius J., Clifton S.W., RA Richardson P.; RT "Genetic and genomic tools for Xenopus research: The NIH Xenopus RT initiative"; RL Dev. Dyn. 225(4):384-391(2002). XX RN [2] RP 1-1320 RX DOI; 10.1073/pnas.242603899 RX PUBMED; 12477932. RG Mammalian Gene Collection Program Team RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., RA Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., RA Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., RA Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K., RA Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F., RA Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S., RA Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J., RA Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J., RA Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M., RA Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., RA Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., RA Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C., RA Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I., RA Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human and RT mouse cDNA sequences"; RL Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002). XX RN [3] RC NIH-MGC Project RP 1-1320 RA Klein S., Strausberg R.; RT ; RL Submitted (27-OCT-2003) to the EMBL/GenBank/DDBJ databases. RL National Institutes of Health, Xenopus Gene Collection (XGC), National RL Institute of Child Health and Human Development, 6100 Executive Boulevard, RL Room 4B01, Rockville, MD 20892-7510, USA XX DR ImaGenes; IMAGp998E189302Q. DR ImaGenes; IRAKp961I24128Q. DR ImaGenes; IRBHp990H0517D. XX CC Contact: XGC help desk CC Email: cgapbs-r@mail.nih.gov CC Tissue Procurement: Dr. Igor Dawid CC cDNA Library Preparation: Life Technologies, Inc. CC cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) CC DNA Sequencing by: National Institutes of Health Intramural CC Sequencing Center (NISC), CC Gaithersburg, Maryland; CC Web site: http://www.nisc.nih.gov/ CC Contact: nisc_mgc@nhgri.nih.gov CC Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B., CC Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S., CC Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P., CC Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R., CC Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C., CC McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W., CC Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L., CC Young,A., Zhang,L.-H. and Green,E.D. CC Clone distribution: MGC clone distribution information can be found CC through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov CC Series: IRAK Plate: 128 Row: i Column: 24 CC This clone was selected for full length sequencing because it CC passed the following selection criteria: Hexamer frequency ORF CC analysis, Similarity but not identity to protein. XX FH Key Location/Qualifiers FH FT source 1..1320 FT /organism="Xenopus laevis" FT /lab_host="DH10B" FT /mol_type="mRNA" FT /clone_lib="NICHD_XGC_Kid1" FT /clone="MGC:68659 IMAGE:4032761" FT /tissue_type="Kidney, adult Xenopus" FT /note="Vector: pCMV-SPORT6" FT /db_xref="taxon:8355" FT gene 1..1320 FT /gene="MGC68659" FT CDS 103..1107 FT /codon_start=1 FT /gene="MGC68659" FT /product="MGC68659 protein" FT /db_xref="GOA:Q6PAB3" FT /db_xref="HSSP:1BDM" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:Q6PAB3" FT /protein_id="AAH60386.1" FT /translation="MPEPVKVLVTGAAGQIAYSLLFGIAKGDVFGKDQPLILVLLDITP FT MMTVLEGVVMELQDCALPLLKEVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKA FT NVKIFKSQGAALNKYSKKSVKVIVVGNPANTNCLTALKSAPSIPKENFSCLTRLDHNRA FT KAQIALKLNVASDDVKNVIIWGNHSSTQYPDASHASVTLQGKDVGAFEAVKNDDWLKGG FT FITTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGQFVSMGVISDGNSYGVP FT EDLMYSFPLTIKNKTWKIVEGLCINDFSREKMDITAKELQDEKETAFEFLSSE" FT misc_feature 106..564 FT /gene="MGC68659" FT /note="Ldh_1_N; Region: lactate/malate dehydrogenase, NAD FT binding domain. L-lactate dehydrogenases are metabolic FT enzymes which catalyse the conversion of L-lactate to FT pyruvate, the last step in anaerobic glycolysis. FT L-2-hydroxyisocaproate dehydrogenases are also members of FT the family. Malate dehydrogenases catalyse the FT interconversion of malate to oxaloacetate. The enzyme FT participates in the citric acid cycle. L-lactate FT dehydrogenase is also found as a lens crystallin in bird FT and crocodile eyes. N-terminus (this family) is a Rossman FT NAD-binding fold. C-terminus is an unusual alpha+beta fold" FT misc_feature 568..1095 FT /gene="MGC68659" FT /note="Ldh_1_C; Region: lactate/malate dehydrogenase, FT alpha/beta C-terminal domain. L-lactate dehydrogenases are FT metabolic enzymes which catalyse the conversion of FT L-lactate to pyruvate, the last step in anaerobic FT glycolysis. L-2-hydroxyisocaproate dehydrogenases are also FT members of the family. Malate dehydrogenases catalyse the FT interconversion of malate to oxaloacetate. The enzyme FT participates in the citric acid cycle. L-lactate FT dehydrogenase is also found as a lens crystallin in bird FT and crocodile eyes" XX SQ Sequence 1320 BP; 384 A; 263 C; 317 G; 356 T; 0 other; ggggcgtaga ggtgaccgca gtcagtctga cacagacagt ttgcagcagc gcagcttgag 60 gccggtcatt atcatctgca cttgtcacgc ttcccaagca aaatgcctga acccgtgaaa 120 gtccttgtga ccggagctgc tggtcagatt gcttactcct tgctctttgg tattgctaaa 180 ggagatgtct ttggaaaaga ccagccactt atacttgttc ttttggatat tacccctatg 240 atgactgtac ttgaaggtgt ggtgatggag ctgcaggact gtgctttgcc cctgctgaaa 300 gaggtgattg ctaccgataa ggaagacgtg gccttcaagg atttggacgt tgccattcta 360 gttggttcta tgccaaggag ggagggcatg gaaagaaaag acttgctgaa agccaatgtg 420 aagatcttta aatctcaggg tgcagctctg aacaagtatt ccaagaagag cgttaaggtt 480 attgttgtgg ggaatccagc aaatacaaac tgcctgactg cactgaagtc tgctccatct 540 atccctaaag agaacttcag ctgtctgaca cgtttagatc acaaccgagc aaaagcacaa 600 attgctctga agttgaatgt tgcatctgat gatgtaaaga acgtaattat atggggtaat 660 cactcatcaa ctcagtatcc tgatgcttct catgcgtctg taactcttca aggaaaagac 720 gttggggcct ttgaagcagt gaagaatgac gactggctaa agggtggatt tatcactact 780 gtgcagcaaa gaggagcagc tgttattaag gccagaaagt tgtccagtgc aatgtctgct 840 gctaaagcca tctgtgatca tgtgagggat atctggtttg gaaccccaga ggggcagttt 900 gtttccatgg gggtcatttc ggatggaaac tcttatggtg tgcctgaaga cctgatgtat 960 tcattccctc ttaccatcaa gaataagacc tggaaaatag tggaaggcct gtgtatcaat 1020 gacttttccc gggagaaaat ggatatcacc gctaaagaac ttcaggatga aaaagaaact 1080 gcatttgaat ttctgtccag cgaatgacta gtgacagatt ccacaatgca cttaaccccc 1140 gagaaaatga aaagttctct ttaagtttca taccaagtgc tatttatgtt gtcaagcaat 1200 ggccacaaag tcacactgga atccattttg tatctgtggc tagtgttcta aatcattcct 1260 agcaatagtc attaaatgaa aaccccacgt ggtttttaaa gtgaaaaaaa aaaaaaaaaa 1320 // |
|