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EBI DbfetchID BC057757; SV 1; linear; mRNA; STD; VRT; 3010 BP. XX AC BC057757; XX DT 18-SEP-2003 (Rel. 77, Created) DT 27-MAY-2005 (Rel. 83, Last updated, Version 3) XX DE Xenopus laevis hypothetical protein MGC69150, mRNA (cDNA clone MGC:69150 DE IMAGE:5048691), complete cds. XX KW MGC. XX OS Xenopus laevis (African clawed frog) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia; OC Batrachia; Anura; Mesobatrachia; Pipoidea; Pipidae; Xenopodinae; Xenopus; OC Xenopus. XX RN [1] RP 1-3010 RX DOI; 10.1002/dvdy.10174 RX PUBMED; 12454917. RA Klein S.L., Strausberg R.L., Wagner L., Pontius J., Clifton S.W., RA Richardson P.; RT "Genetic and genomic tools for Xenopus research: The NIH Xenopus RT initiative"; RL Dev. Dyn. 225(4):384-391(2002). XX RN [2] RP 1-3010 RX DOI; 10.1073/pnas.242603899 RX PUBMED; 12477932. RG Mammalian Gene Collection Program Team RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., RA Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., RA Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., RA Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K., RA Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F., RA Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S., RA Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J., RA Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J., RA Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M., RA Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., RA Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., RA Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C., RA Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I., RA Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human and RT mouse cDNA sequences"; RL Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002). XX RN [3] RC NIH-MGC Project RP 1-3010 RA Klein S., Strausberg R.; RT ; RL Submitted (03-SEP-2003) to the EMBL/GenBank/DDBJ databases. RL National Institutes of Health, Xenopus Gene Collection (XGC), National RL Institute of Child Health and Human Development, 6100 Executive Boulevard, RL Room 4B01, Rockville, MD 20892-7510, USA XX DR ImaGenes; IMAGp998P0411132Q. DR ImaGenes; IRAKp961M21129Q. DR ImaGenes; IRBHp990B1215D. XX CC Contact: XGC help desk CC Email: cgapbs-r@mail.nih.gov CC Tissue Procurement: Dr. Igor Dawid CC cDNA Library Preparation: Life Technologies, Inc. CC cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) CC DNA Sequencing by: Sequencing Group at the Stanford Human Genome CC Center, Stanford University School of Medicine, Stanford, CA 94305 CC Web site: http://www-shgc.stanford.edu CC Contact: (Dickson, Mark) mcd@paxil.stanford.edu CC Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers, CC R. M. CC Clone distribution: MGC clone distribution information can be found CC through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov CC Series: IRAK Plate: 129 Row: m Column: 21 CC This clone was selected for full length sequencing because it CC passed the following selection criteria: Hexamer frequency ORF CC analysis, Similarity but not identity to protein. XX FH Key Location/Qualifiers FH FT source 1..3010 FT /organism="Xenopus laevis" FT /lab_host="DH10B" FT /mol_type="mRNA" FT /clone_lib="NICHD_XGC_Ov1" FT /clone="MGC:69150 IMAGE:5048691" FT /tissue_type="Ovary, Xenopus" FT /note="Vector: pCMV-SPORT6" FT /db_xref="taxon:8355" FT gene 1..3010 FT /gene="MGC69150" FT CDS 134..2851 FT /codon_start=1 FT /product="MGC69150 protein" FT /db_xref="GOA:Q6PF21" FT /db_xref="InterPro:IPR000738" FT /db_xref="UniProtKB/Swiss-Prot:Q6PF21" FT /protein_id="AAH57757.1" FT /translation="MKLFVGEGNPQGVKVLAAAALWAQHVQIDRLQQEEKIVPFMSQPR FT LPVLDLENGNYLFLSNAICRYFYLSSGHDMCDLSNQWLEWEAAELQPALSAALYAHVVQ FT GKKKEDVMATISASLKHLDQSLAGKSSPYLIKDALTVVDIVVWGSIYPLIVDASNLPEE FT MASLKRWFQNVSQLEQCQKAASSLLKDKGSSVFKPFLQKQPAPITPPGKSVCKEQEGED FT MPSLSEEDIQAAAEAWAKGLTGASKPKQRPHPILPVEGEKNVLITSALPYVNNVPHLGN FT IIGSVLSADVFARYCRLRNWNTLYICGTDEYGTATETKAMEEGLTPQQICDKYNAIHTA FT IYQWFNISFDYFGRTTTQHQTTISQDIFHRLLEREFLLTDTVEQLRCEKCQRFLADRFV FT EGICPFCNYEEARGDQCDKCGKLINAVELKKPQCKICKQSPVIKSSKHLFLDLPKLEKR FT LEQWLEQSFSTGDWTSNARFITRSWIRDGLKPRCITRDLKWGTPVPLDGFRDKVFYVWF FT DAPIGYISITANYTDQWEKWWKSPQQVQLYNFMAKDNVPFHSVVFPSCLLGAEDNYTLV FT NHLVATEYLNYEDGKFSKSRGVGVFGDMAKDTGIPADIWRFYLLYVRPEGQDSAFSWSD FT LMLKNNSELLNNLGNFVNRAGMFVQKFFNGCVPEMELLSEDKRLLAQVAAELQQYNLLL FT EKVRIRDALRCILNISRHGNQYIQVNEPWKCIKGNQQEQKRAGTVTGVAVNMAALLSIM FT LHPYMPTISSVIQEQLLMPQESKVLTTDFCCCLQSGHQIGNVSPLFQKLENDQIESLRK FT RFGGGQVKTESKVSPSQEAPEQQAPKASGPERVKELMQELEKQGNHVRELKGKKAEKSV FT IDPEVQKLLALKKELALAEGKSPDPPTQKGKKKK" FT misc_feature 134..334 FT /note="GST_N; Region: Glutathione S-transferase, N-terminal FT domain. Function: conjugation of reduced glutathione to a FT variety of targets. Also included in the alignment, but are FT not GSTs: * S-crystallins from squid. Similarity to GST FT previously noted. * Eukaryotic elongation factors 1-gamma. FT Not known to have GST activity" FT misc_feature 371..667 FT /note="GST_C; Region: Glutathione S-transferase, C-terminal FT domain. Function: conjugation of reduced glutathione to a FT variety of targets. Also included in the alignment, but are FT not GSTs: * S-crystallins from squid. Similarity to GST FT previously noted. * Eukaryotic elongation factors 1-gamma. FT Not known to have GST activity" FT misc_feature 908..1333 FT /note="tRNA-synt_1; Region: tRNA synthetases class I (I, L, FT M and V). Other tRNA synthetase sub-families are too FT dissimilar to be included" FT misc_feature 1352..2029 FT /note="tRNA-synt_1; Region: tRNA synthetases class I (I, L, FT M and V). Other tRNA synthetase sub-families are too FT dissimilar to be included" FT misc_feature 2675..2764 FT /note="WHEP-TRS; Region: WHEP-TRS domain" XX SQ Sequence 3010 BP; 866 A; 648 C; 736 G; 760 T; 0 other; cgatggtaga acgtcgtgcg ggactctagt tcccaatgca cgttgcgaca cacgtgtggc 60 cggttttcat gtgagttgca tcagctgtgc ctgttgcact gtttgccgct agggaagtgt 120 ctgatcgctg accatgaagt tgtttgtggg tgaggggaac cctcaggggg tgaaggtgct 180 ggctgccgcc gctctctggg ctcaacatgt acagatcgac aggctacagc aggaagaaaa 240 gattgttcct tttatgtcac aaccaagact accagtgctg gacttggaaa atggaaatta 300 tctcttctta tctaatgcaa tctgcaggta cttctacttg tcatctggtc atgatatgtg 360 cgatctaagc aatcagtggt tggaatggga agcagccgag cttcagccag cactctctgc 420 tgcgttatat gcacatgttg ttcaggggaa aaaaaaggaa gatgtcatgg caaccattag 480 tgcatccctt aagcatttgg atcaaagcct tgcaggaaaa tccagtccat accttatcaa 540 ggatgcactc actgttgtgg atattgttgt gtggggctct atctatcctc taattgtgga 600 tgcatctaat cttccagaag agatggcatc acttaagcgc tggtttcaaa atgttagtca 660 gctagaacag tgccaaaaag ctgcgtcatc gctgttgaaa gataagggga gctcagtctt 720 caagcctttc ctccagaagc agcctgcacc cataacccca cctggcaagt ccgtttgtaa 780 ggagcaagag ggagaggaca tgccgtctct gtctgaggag gatatacaag ctgctgctga 840 agcttgggct aaaggtctca ctggtgcttc aaagccaaag cagagaccac acccaatcct 900 gccagtggaa ggagaaaaaa acgttctcat caccagtgct ttgccctatg tcaataacgt 960 tccgcacctt ggaaatatca ttggttctgt gctcagcgca gatgtttttg ctaggtactg 1020 caggctgcgg aactggaaca cactctacat atgtggaacg gatgaatatg gcacagcaac 1080 agaaaccaaa gctatggagg aagggttgac accccaacag atctgtgaca agtacaatgc 1140 tatccacact gcgatatatc agtggtttaa catctctttt gactattttg gacggacaac 1200 aacccaacac caaaccacta tttcccaaga tatctttcat cgacttctcg agagggaatt 1260 tttactgaca gacacagtgg aacagcttcg ctgtgagaaa tgccagaggt tcttggctga 1320 tcgctttgtg gaaggaatat gtcctttctg taactacgaa gaggcgagag gggaccaatg 1380 tgacaaatgt gggaagctca taaatgctgt agagttaaag aaaccacagt gcaaaatttg 1440 taagcagtcc cctgtcatta aatcctccaa gcatctcttc ttggacttgc caaagctgga 1500 gaaacgtttg gaacaatggt tggaacagtc cttctctacc ggtgactgga catcaaatgc 1560 ccgtttcata actcgctcat ggatacgtga tggactgaag cctcgttgta tcacacgaga 1620 tctgaaatgg ggcacacctg tgccactgga tggtttcaga gacaaggtgt tctatgtctg 1680 gtttgatgct cccattggct atatttctat tacagccaac tatacagatc agtgggaaaa 1740 gtggtggaag agtcctcaac aggtccagct ttataatttt atggcaaagg acaatgttcc 1800 tttccacagt gttgtcttcc cttcctgcct tttgggtgca gaagataact acacattggt 1860 caaccacctt gtagctacag aatatttaaa ttatgaggat ggtaaattct ccaagagtcg 1920 aggagttggg gtatttggag atatggcaaa ggacactggt atccctgcgg atatatggag 1980 attctaccta ctttatgtga gaccagaagg acaagacagt gctttcagct ggagtgatct 2040 catgctcaaa aacaactctg agctcctcaa caaccttggc aattttgtca acagggctgg 2100 tatgtttgtg cagaagtttt tcaatggctg tgttcctgaa atggagctgc tttcagaaga 2160 taagaggctt ctggcccaag ttgcagcaga actgcagcag tacaacctgc ttctggagaa 2220 agttcgcatc cgagatgctc ttcgatgtat tctgaacatt tcacgtcatg gcaatcagta 2280 catccaagtt aacgaaccat ggaagtgcat caaaggaaac caacaagagc agaagagagc 2340 aggcacagtg actggagtgg ctgtaaacat ggccgctctt ctctccatta tgttgcatcc 2400 atacatgcca acaatcagca gtgttatcca ggagcagctg ctaatgccac aagaatctaa 2460 agtgttaacc accgacttct gctgttgtct gcaaagtggc caccaaattg gaaatgtgag 2520 tccactgttc cagaagttgg agaacgacca gatagaatcc ttgcgcaaac gctttggagg 2580 agggcaggta aaaacagagt caaaagtgtc tccatctcag gaagctcctg agcagcaagc 2640 acctaaagcc tctgggccag aaagagtgaa ggagctgatg caggagctgg aaaaacaggg 2700 taaccatgtg cgggagctaa aggggaaaaa agccgaaaaa tcagtcattg accctgaggt 2760 gcaaaagctt ttagcattaa agaaagaact ggcgctcgca gaaggcaagt caccggatcc 2820 tcccactcag aagggcaaga aaaagaaata gactttttgt ttgtttgctt gtgcatcttg 2880 tattttatga gctggtcagc aatggatggg ccagtgatac tttgtgtaga cacatcacat 2940 cagggagaaa gtgaaacaag ggaacactct tttaaattaa atattttggt cattcaaaaa 3000 aaaaaaaaaa 3010 // |
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