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EBI DbfetchID BC050288; SV 1; linear; mRNA; STD; HUM; 2479 BP. XX AC BC050288; XX DT 23-APR-2003 (Rel. 75, Created) DT 15-OCT-2008 (Rel. 97, Last updated, Version 10) XX DE Homo sapiens DnaJ (Hsp40) homolog, subfamily B, member 8, mRNA (cDNA clone DE MGC:43650 IMAGE:5270636), complete cds. XX KW MGC. XX OS Homo sapiens (human) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. XX RN [1] RP 1-2479 RX DOI; 10.1073/pnas.242603899 RX PUBMED; 12477932. RG Mammalian Gene Collection Program Team RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., RA Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., RA Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., RA Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K., RA Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F., RA Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S., RA Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J., RA Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J., RA Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M., RA Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., RA Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., RA Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C., RA Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I., RA Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human and RT mouse cDNA sequences"; RL Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002). XX RN [2] RC NIH-MGC Project URL: http://mgc.nci.nih.gov RP 1-2479 RG NIH MGC Project RA ; RT ; RL Submitted (08-APR-2003) to the EMBL/GenBank/DDBJ databases. RL National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda, RL MD 20892-2590, USA XX DR ASTD; TRAN00000046744. DR Ensembl-Gn; ENSG00000179407; Homo_sapiens. DR Ensembl-Tr; ENST00000319153; Homo_sapiens. DR H-InvDB; HIT000259098. DR ImaGenes; IMAGp998O2111682Q. DR ImaGenes; IRAKp961L1874Q. DR ImaGenes; IRATp970C11104D. XX CC Contact: MGC help desk CC Email: cgapbs-r@mail.nih.gov CC Tissue Procurement: Miklos Palkovits, M.D., Ph.D. CC cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki CC Toshiyuki and Piero Carninci (RIKEN) CC cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) CC DNA Sequencing by: Sequencing Group at the Stanford Human Genome CC Center, Stanford University School of Medicine, Stanford, CA 94305 CC Web site: http://www-shgc.stanford.edu CC Contact: (Dickson, Mark) mcd@paxil.stanford.edu CC Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers, CC R. M. CC Clone distribution: MGC clone distribution information can be found CC through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov CC Series: IRAK Plate: 74 Row: l Column: 18 CC This clone was selected for full length sequencing because it CC passed the following selection criteria: matched mRNA gi: 46094077. CC Differences found between this sequence and the human reference CC genome (build 36) are described in misc_difference features below CC and these differences were also compared to chimpanzee genomic CC sequences available as of 09/15/2004. XX FH Key Location/Qualifiers FH FT source 1..2479 FT /organism="Homo sapiens" FT /lab_host="DH10B" FT /mol_type="mRNA" FT /clone_lib="NIH_MGC_97" FT /clone="MGC:43650 IMAGE:5270636" FT /tissue_type="Testis" FT /note="Vector: pBluescriptR" FT /db_xref="taxon:9606" FT gene 1..2479 FT /gene="DNAJB8" FT /note="synonym: MGC33884" FT misc_difference 200 FT /gene="DNAJB8" FT /note="'C' in cDNA is 'A' in the human genome. The FT chimpanzee genome agrees with the cDNA sequence, suggesting FT that this difference is unlikely to be due to an artifact." FT CDS 1662..2360 FT /codon_start=1 FT /gene="DNAJB8" FT /product="DnaJ (Hsp40) homolog, subfamily B, member 8" FT /db_xref="GDB:11513595" FT /db_xref="GOA:Q8NHS0" FT /db_xref="HGNC:23699" FT /db_xref="InterPro:IPR015609" FT /db_xref="PDB:2DMX" FT /db_xref="UniProtKB/Swiss-Prot:Q8NHS0" FT /protein_id="AAH50288.1" FT /translation="MANYYEVLGVQASASPEDIKKAYRKLALRWHPDKNPDNKEEAEKK FT FKLVSEAYEVLSDSKKRSLYDRAGCDSWRAGGGASTPYHSPFDTGYTFRNPEDIFREFF FT GGLDPFSFEFWDSPFNSDRGGRGHGLRGAFSAGFGEFPAFMEAFSSFNMLGCSGGSHTT FT FSSTSFGGSSSGSSGFKSVMSSTEMINGHKVTTKRIVENGQERVEVEEDGQLKSVTVNG FT KEQLKWMDSK" FT misc_difference 1973 FT /gene="DNAJB8" FT /note="'C' in cDNA is 'T' in the human genome; no amino FT acid change. The chimpanzee genome agrees with the cDNA FT sequence, suggesting that this difference is unlikely to be FT due to an artifact." FT misc_difference 2009 FT /gene="DNAJB8" FT /note="'T' in cDNA is 'C' in the human genome; no amino FT acid change. The chimpanzee genome agrees with the human FT genomic sequence and not the cDNA." FT misc_difference 2466..2479 FT /gene="DNAJB8" FT /note="polyA tail: 14 bases do not align to the human FT genome." XX SQ Sequence 2479 BP; 527 A; 829 C; 629 G; 494 T; 0 other; acaccgcctt tgccatcacc accgtcattg ccccctctac taccaccacc atcaccacca 60 ccgggtctgc ctcagccaga cccaccactg cctctaatta gactgccacc atcatcactg 120 ccaccaccac tgcccctcca atgccttcct gatgtcacca tctgctttct ctctgctatc 180 ctcacaccca actccatcac aaccaccacc ttcatctgag ccgccatcac cctcacagta 240 cacagccctc aaatgtgtac caaggaggag gacatttact acacgctgca ccctgaacag 300 tctgggacca gcggtcaggg aacaaggaat cgagatgctc acctgcagct cccaggtgag 360 cggctctcta gagcttgcct gggagctgct gaggagctca cggtattcca ggaagctccc 420 catccatgcc tcagcctgtg gctcagaagc agggtcttcg cagaagattg ccccgagctg 480 ttgcaaagct caccattgtc acctgcctgc aacggcctct cttttccact ctccaaatcc 540 cttgtattgg caggctctag tctggaatta taccggtgat gggattctgg aaaatccctc 600 agatttctcc aggatgcaag gagaccatgg aagaggaaca agcctgcaag ggacagcagg 660 tgctaactgc taaccgagac atagtgacaa aaatccagcc acagagataa agaatcaggt 720 tcatctgtag ctacatccag tggagaagtt ctgctcttca actcctctgc taagccacat 780 gtgtcagcag gtgtagaatt gagtggaaca cttctttgga tttgctgtcc gctgcagcct 840 tggccctggt ttcacatccc ctccctcact gactcgctgt gtgaccttga gcactgtgtg 900 tctctgagcc tggttcatcc aagagtttac cgacggccct cgtgtgccag ccactgtcct 960 agacagggac agactttccc gcctttgtgg agcttacccc ggaagacctg gccagataat 1020 gggcacaaaa aaagcaggct cttgtcccca ccccagcctg cctgaacccc accttggcct 1080 cctcttgcct cagctgctgc ccagccatcg gccgagtgga catacccagg acgcccttgc 1140 ccgcctcccc caccacggcc acagaccttt gtcactcacc aaggctggaa tcccgtggct 1200 gatcctgcct ctgccctttg gctcccggga ctcagcccac accacctggg tcacagagca 1260 tcccattccc acacaccgtt gtggccacct caccagcagg ggcaggccca tatgcccagg 1320 tttgcctggt gaggagctgg gggcgggggt atgccccgcc ccgggagctg acgtcataaa 1380 aggagctctg gagggcagcc cactctggcc tggccccaca gcggcagtgt ccctcccctc 1440 cccccactcc tctcagtggg ggcccctcca gtccctgaga attggtacta cgaaaaggtg 1500 aactcctggg cagaatcttg cctagagctt gcggagtcca gccaggcccc tgctgaaggg 1560 ccccagacca ccggccactt ctcccccgtc catctgacca gctgggcccc tgcgcccacc 1620 tggcctccac gttccctctc ctctcaccca cacccctggc catggctaac tactacgaag 1680 tgctgggcgt gcaggccagc gcttccccgg aggacatcaa gaaagcctac cgcaagctgg 1740 cccttcgttg gcaccccgac aagaaccctg acaataagga ggaggcggag aagaagttca 1800 agctggtgtc tgaggcctat gaggttctgt ctgactccaa gaaacgctcc ctgtatgacc 1860 gtgctggctg tgacagctgg cgggctggtg gcggggccag cacgccctac cacagcccct 1920 tcgacaccgg ctacaccttc cgtaaccctg aggacatctt ccgggagttt ttcggtggcc 1980 tggacccttt ctcctttgag ttctgggata gcccattcaa tagtgaccgt ggtggccggg 2040 gccatggcct gaggggggcc ttctcggcag gctttggaga atttccggcc ttcatggagg 2100 ccttctcatc cttcaacatg ctgggctgca gcgggggcag ccacaccacc ttctcatcca 2160 cctccttcgg gggctccagt tctggcagct cggggttcaa gtcggtgatg tcgtccaccg 2220 agatgatcaa tggccacaag gtcaccacca agcgcatcgt ggagaacggg caggagcgcg 2280 tggaggtgga ggaagacggg cagctcaagt cggtgactgt gaacggcaag gagcagctca 2340 aatggatgga cagcaagtag gcgctggcca cccggccctg ccttcccacc accaccaccg 2400 tgcatggggc agcaaacacg tggggccgca gacatagcct gatggttaat aaatgtgcca 2460 agtgaaaaaa aaaaaaaaa 2479 // |
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