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EBI DbfetchID BC047502; SV 1; linear; mRNA; STD; HUM; 3481 BP. XX AC BC047502; XX DT 04-MAR-2003 (Rel. 75, Created) DT 15-OCT-2008 (Rel. 97, Last updated, Version 12) XX DE Homo sapiens protein phosphatase 1, regulatory (inhibitor) subunit 3D, mRNA DE (cDNA clone MGC:48529 IMAGE:5312727), complete cds. XX KW MGC. XX OS Homo sapiens (human) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. XX RN [1] RP 1-3481 RX DOI; 10.1073/pnas.242603899 RX PUBMED; 12477932. RG Mammalian Gene Collection Program Team RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., RA Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., RA Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., RA Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K., RA Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F., RA Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S., RA Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J., RA Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J., RA Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M., RA Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., RA Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., RA Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C., RA Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I., RA Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human and RT mouse cDNA sequences"; RL Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002). XX RN [2] RC NIH-MGC Project URL: http://mgc.nci.nih.gov RP 1-3481 RG NIH MGC Project RA ; RT ; RL Submitted (28-FEB-2003) to the EMBL/GenBank/DDBJ databases. RL National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda, RL MD 20892-2590, USA XX DR H-InvDB; HIT000053147. DR ImaGenes; IMAGp998I1611792Q. DR ImaGenes; IRAKp961M1391Q. DR ImaGenes; IRATp970A0463D. XX CC Contact: MGC help desk CC Email: cgapbs-r@mail.nih.gov CC Tissue Procurement: Miklos Palkovits, M.D., Ph.D. CC cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki CC Toshiyuki and Piero Carninci (RIKEN) CC cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) CC DNA Sequencing by: Baylor College of Medicine Human Genome CC Sequencing Center CC Center code: BCM-HGSC CC Web site: http://www.hgsc.bcm.tmc.edu/cdna/ CC Contact: amg@bcm.tmc.edu CC Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H., CC Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati, CC A.N., Gibbs, R.A. CC Clone distribution: MGC clone distribution information can be found CC through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov CC Series: IRAK Plate: 91 Row: m Column: 13 CC This clone was selected for full length sequencing because it CC passed the following selection criteria: matched mRNA gi: 37622904. CC Differences found between this sequence and the human reference CC genome (build 36) are described in misc_difference features below CC and these differences were also compared to chimpanzee genome CC (build 1). XX FH Key Location/Qualifiers FH FT source 1..3481 FT /organism="Homo sapiens" FT /lab_host="DH10B" FT /mol_type="mRNA" FT /clone_lib="NIH_MGC_96" FT /clone="MGC:48529 IMAGE:5312727" FT /tissue_type="Brain, hypothalamus" FT /note="Vector: pBluescriptR" FT /db_xref="taxon:9606" FT gene 1..3481 FT /gene="PPP1R3D" FT CDS 367..1266 FT /codon_start=1 FT /gene="PPP1R3D" FT /product="protein phosphatase 1, regulatory (inhibitor) FT subunit 3D" FT /db_xref="HGNC:9294" FT /db_xref="InterPro:IPR005036" FT /db_xref="InterPro:IPR017434" FT /db_xref="UniProtKB/TrEMBL:Q86X09" FT /protein_id="AAH47502.1" FT /translation="MSRGPSSAVLPSALGSRKLGPRSLSCLSDLDGGVALEPRACRPPG FT SPGRAPPPTPAPSGCDPRLRPIILRRARSLPSSPERRQKAAGAPGAACRPGCSQKLRVR FT FADALGLELAQVKVFNAGDDPSVPLHVLSRLAINSDLCCSSQDLEFTLHCLVPDFPPPV FT EAADFGERLQRQLVCLERVTCSDLGISGTVRVCNVAFEKQVAVRYTFSGWRSTHEAVAR FT WRGPAGPEGTEDVFTFGFPVPPFLLELGSRVHFAVRYQVAGAEYWDNNDHRDYSITCRN FT HALHMPRGECEESWIHFI" FT misc_difference 1056 FT /gene="PPP1R3D" FT /note="'A' in cDNA is 'G' in the human genome; no amino FT acid change. The chimpanzee genome agrees with the human FT genomic sequence and not the cDNA." FT misc_difference 1183 FT /gene="PPP1R3D" FT /note="'A' in cDNA is 'C' in the human genome; no amino FT acid change. The chimpanzee genome agrees with the human FT genomic sequence and not the cDNA." FT misc_difference 1195 FT /gene="PPP1R3D" FT /note="'A' in cDNA is 'C' in the human genome; amino acid FT difference: 'I' in cDNA, 'L' in the human genome. The FT chimpanzee genome agrees with the human genomic sequence FT and not the cDNA." FT misc_difference 1281 FT /gene="PPP1R3D" FT /note="'A' in cDNA is 'G' in the human genome. The FT chimpanzee genome agrees with the human genomic sequence FT and not the cDNA." FT misc_difference 1666 FT /gene="PPP1R3D" FT /note="1 base in cDNA is not found in the human genome. The FT chimpanzee genome agrees with the human genomic sequence FT and not the cDNA." FT misc_difference 1783^1784 FT /gene="PPP1R3D" FT /note="1 base in the human genome, G, is not found in cDNA. FT The chimpanzee genome agrees with the human genomic FT sequence and not the cDNA." FT misc_difference 1785 FT /gene="PPP1R3D" FT /note="'A' in cDNA is 'C' in the human genome. The FT chimpanzee genome agrees with the human genomic sequence FT and not the cDNA." FT misc_difference 1985 FT /gene="PPP1R3D" FT /note="'G' in cDNA is 'A' in the human genome. The FT chimpanzee genome agrees with the human genomic sequence FT and not the cDNA." FT misc_difference 2484 FT /gene="PPP1R3D" FT /note="'A' in cDNA is 'C' in the human genome. The FT chimpanzee genome agrees with the human genomic sequence FT and not the cDNA." FT misc_difference 2501 FT /gene="PPP1R3D" FT /note="'G' in cDNA is 'A' in the human genome. The FT chimpanzee genome agrees with the human genomic sequence FT and not the cDNA." FT misc_difference 3467..3481 FT /gene="PPP1R3D" FT /note="polyA tail: 15 bases do not align to the human FT genome." XX SQ Sequence 3481 BP; 860 A; 869 C; 874 G; 878 T; 0 other; aagtaacgcc gcggaccggg aaagtgggag gggccgctcc cggaacgcag ccttcttgta 60 agaacctcca aggaagcaag aagaaaaaag aggcgctacc ctgcgctccc gggaggcctc 120 tgaaagcttc cactagagaa aaactcccca ctcttacaat ttctttaacc gcaagaagcg 180 gaggacctgg acaaggactc gaggagcaag gtggcgaacc aagggtaggg cgcaccgggc 240 ccgagaggtc ccccgcaggt tgcagatacg gtggactctc tgcggcttct gagcacggag 300 ggagctgtcg cggggtcggg aggtcgtctc tcatccctgc ctcatcttcc gacggccggc 360 ggggccatgt ccagaggccc gagctccgcg gtcctgccta gcgccctggg atcccggaag 420 ctcggccccc ggagcctcag ctgcctgtcg gacctggacg gcggcgtggc cctggagccg 480 cgggcctgta ggccccctgg gagcccgggc cgcgcgccgc cgccaacgcc agcgccgtcg 540 ggctgcgacc cccgcctgcg gcccatcatc ctgcggcggg cgcgctcact gcccagctcc 600 cccgagcgcc gccagaaggc cgcgggcgcg ccgggcgctg cgtgtcggcc gggctgcagc 660 cagaagctcc gcgtgcgctt cgccgacgcc ctgggcttgg agctggcaca ggtcaaggtg 720 ttcaacgcgg gagacgaccc gtccgtgccg ctgcacgtgc tgtcgcggct cgcaatcaac 780 tcggacctgt gctgcagcag ccaggacctg gagttcaccc tgcattgcct ggtgcccgat 840 ttcccgccgc ccgtcgaggc cgccgacttt ggcgagcgcc tgcagcggca gctcgtgtgc 900 ctggagcgtg tcacttgctc ggaccttggc atcagcggta cggtgcgcgt gtgcaacgtg 960 gccttcgaga agcaggtggc tgtgcgctac actttctcgg gctggcgcag tacccacgag 1020 gcggtggcgc ggtggcgcgg gcccgcaggc cccgaaggca cggaggacgt tttcaccttc 1080 ggctttccag taccgccctt cctgctggag ctcggctccc gcgtgcactt cgcggtgcgc 1140 taccaagtgg cgggtgccga gtactgggac aacaacgacc acagagacta cagcatcaca 1200 tgtcgcaacc acgcgctgca catgcctcgc ggggagtgcg aagagagctg gatccacttc 1260 atctgagccg cgcggggacc agccacctgg agcctccaca cctaagctgc gcctcctgtc 1320 atttccctgc tgggctctca catctatctg gttgttcttc accaccctcc aagtcctctg 1380 acctaatttt ctgctgcaag gtcccctggc agtggcccat cctgtcttct acttgaaacg 1440 tctgagtcac ttggtctaaa aagtagcctc aggtggccag aaggccgagt tgtgtaatga 1500 gttggggcag ggtggtgggg taggtgcatg ggagggtggg ccctctgggg aggcccaagc 1560 agcttgtttt gcaaaggccc aagtcctcct gctaggaaaa gcttttgcat gtgtcctgaa 1620 tgtgtctctg taaacatagc tgttatttat tattgtgatg ttggggacct ttagcccata 1680 gcggatgcct cctcaggaat attctcgatt taataagcta aaaaaaaaaa gttctgtgtt 1740 tgtgcccaca gtaaacagaa tgtacagtgt gacccacatc gtgtacctcc aggcattcct 1800 gccccaggat aaggaaaacc tgagcccaca tctgattgcc aacggctgta gccttctaac 1860 agtgggaaac tattaatagg caagaaattt ctgctttatc agtcatcaca caaccattcc 1920 tgggcattga gactgtcctg gttaggctgt gtgtctccta cattaacagc tggggaggag 1980 ggaagagctt cagactttta aactagcaaa acagttattt ctctgcattc acaattctga 2040 tttcatgtat ttctctgtac atgtttgatc attatcaggc cattctgttt aagtttgaaa 2100 ctaagcattt taagaagtct tgcctcagtt atttcagttt tcaggtgact agagaatttc 2160 aggcaacagt gccctttgca attacctttt tgatgattaa ggtgactatt tttatttctt 2220 gttaagcatc agaagatgtc agcattcatc ttttgggagt ccagttacaa ggtggaacta 2280 gaaggtgttt tcagcaatag ccagagcact ggaggaaaaa taattgacaa atatttatac 2340 ttttaaattg ttttgaactg tatttacata catcatgttt tatcaggtac ccaccaactt 2400 tatactttct gccttcagat agagtttcta acattttcta gtttgtcagt tttaaaatat 2460 aaaattcttt tatgttttta aacagtcaac caagaagtca ggaccactgt cccctgctga 2520 gttaggcaat cacttaaaac tccaaagaac tccaggctga ggagttcttt tggtgctgaa 2580 cagtggtctg acaacaggtg ctagaattaa ggtgtgggaa aaaaaaaaaa acagccagga 2640 aggacaagat ctccaatttg tgaagcttat gatacagctg aaaccagtct gatcaatttt 2700 atgctacctg tcaccaagaa attaactcta aatttttgaa tgcttgttgg catctgtcaa 2760 atcaatactg aaaggatgca tttcggcttt taaccagttt ggttctgatc attgcaacct 2820 ctgtgctttt ataattcaaa gaacaaggat agctttcttc ggtatctttt aatataagaa 2880 cactgaattt tggcttgaaa tattgtgact aaattgcaca atctctggct attagcactg 2940 ttcactacat taacatgcaa gagagagaga agccttgtta catttcctgc tatttaacaa 3000 actgtccaat taggtcagca agcctgttag ggccttcact gctacgcccc tggccccaaa 3060 acagagagca agacagttgt ccagagaatt tagatatgag ttaagagctg ccagatttat 3120 tttaaggcat gtagagaatc tcataatcta tgtgaaagtc acttgccaaa aaggttttgg 3180 ttatgaaagt gaatgttata tatttaatat taagtgaacc tcccgtgcct tttaaaaaat 3240 accaacatta tgaaatttaa aagcttaaat atatttgcct agttgataaa gaggctgtgg 3300 aacaactgaa ggagaacttt cagaccctaa aacagttata ttacttagat gttaaaattt 3360 tctagtgggc tgtgcaattg ctgggttgtc cccccaaaca tattttaata tgttatttaa 3420 aatgatttta cccaagccta agtattaaag tatatgtgca aaaacaaaaa aaaaaaaaaa 3480 a 3481 // |
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