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EBI DbfetchID BC045461; SV 1; linear; mRNA; STD; VRT; 3352 BP. XX AC BC045461; XX DT 11-FEB-2003 (Rel. 74, Created) DT 21-OCT-2008 (Rel. 97, Last updated, Version 13) XX DE Danio rerio ER degradation enhancer, mannosidase alpha-like 1, mRNA (cDNA DE clone MGC:55816 IMAGE:3817790), complete cds. XX KW MGC. XX OS Danio rerio (zebrafish) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; OC Cyprinidae; Danio. XX RN [1] RP 1-3352 RX DOI; 10.1073/pnas.242603899 RX PUBMED; 12477932. RG Mammalian Gene Collection Program Team RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., RA Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., RA Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., RA Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K., RA Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F., RA Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S., RA Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J., RA Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J., RA Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M., RA Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., RA Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., RA Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C., RA Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I., RA Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human and RT mouse cDNA sequences"; RL Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002). XX RN [2] RC NIH-MGC Project URL: http://mgc.nci.nih.gov RP 1-3352 RG NIH MGC Project RA ; RT ; RL Submitted (31-JAN-2003) to the EMBL/GenBank/DDBJ databases. RL National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda, RL MD 20892-2590, USA XX DR Ensembl-Gn; ENSDARG00000025094; Danio_rerio. DR Ensembl-Tr; ENSDART00000088961; Danio_rerio. DR ImaGenes; IMAGp998H159107Q. DR ImaGenes; IRAKp961I19102Q. DR ImaGenes; IRBOp991D0916D. XX CC Contact: MGC help desk CC Email: cgapbs-r@mail.nih.gov CC Tissue Procurement: Dr. Sumio Sugano and Dr. Ko-ichi Kawakami CC cDNA Library Preparation: Dr. Sumio Sugano and Dr. Ko-ichi Kawakami CC cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) CC DNA Sequencing by: Institute for Systems Biology CC http://www.systemsbiology.org CC contact: amadan@systemsbiology.org CC Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha CC Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting CC Clone distribution: MGC clone distribution information can be found CC through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov CC Series: IRAK Plate: 102 Row: i Column: 19 CC This clone was selected for full length sequencing because it CC passed the following selection criteria: matched mRNA gi: 28279609. XX FH Key Location/Qualifiers FH FT source 1..3352 FT /organism="Danio rerio" FT /lab_host="DH10B" FT /strain="AB" FT /mol_type="mRNA" FT /clone_lib="Sugano Kawakami zebrafish DRA" FT /clone="MGC:55816 IMAGE:3817790" FT /tissue_type="Whole body, adult, (one male and one female, FT including unfertilized eggs)" FT /note="Vector: pME18S-FL3" FT /db_xref="taxon:7955" FT gene 1..3352 FT /gene="edem1" FT /note="synonyms: MGC55816, zgc:55816" FT CDS 63..1637 FT /codon_start=1 FT /gene="edem1" FT /product="ER degradation enhancer, mannosidase alpha-like FT 1" FT /db_xref="GOA:Q7ZVP7" FT /db_xref="HSSP:1FO3" FT /db_xref="InterPro:IPR001382" FT /db_xref="UniProtKB/TrEMBL:Q7ZVP7" FT /db_xref="ZFIN:ZDB-GENE-040426-808" FT /protein_id="AAH45461.1" FT /translation="MQWRSIVVGLLVLRVLLNCAVWLVFGLGPSWGFDFPIKFTFNSHK FT IELFNDGERATVKTNAWLRHLYDSRNPQAKTCSKISGNSPKKSYLSFFEEGRDEYDRKY FT SSFPDALKVKMRAMARDMFYFGYDNYMKFAFPEDELNPIACEGRGPDVLNPSNININDV FT LGNYSLTLIDTLDTLLVLGNVTEFHRAVKLVIDTVSFDKDSTVQVFEANIRILGSLISA FT HILLTDPRHPFGDVGLKDYDNELLHLAHDLAVRLLPAFENTSTGIPYPRVNLKNGVPPD FT SINETCTAGAGSLLVEFGILSRLIGDSTFEWVARRAVKALWRLRSNETGLLGNVVNIQT FT GQWVGKQSGLGAGMDSFYEYLLKSYILFGEKEDFRMFTAAYESIQSHLRRGREACNEGE FT GDPPLYVNVNMFNGQIMNTWIDSLQAFFPGLQVLNGDVENAICLHAFYYAIWKRFGALP FT ERYNWQLQAPDVLFYPLRPELVESTYLLYQATKNPFYLHVGMDILESLEKKYQSEVWLR FT YSSSCSG" XX SQ Sequence 3352 BP; 945 A; 663 C; 726 G; 1018 T; 0 other; gcgcgtcgtt aagtcgcagc ggctaagtta attatgactt tgaaggccga cagcaggcaa 60 agatgcaatg gaggtcaata gtcgtgggtc tccttgtttt aagagtgctg ctcaattgcg 120 cggtgtggct cgtgtttgga ctgggaccca gctggggctt tgacttcccc attaaattca 180 cgttcaactc gcataaaatt gaacttttca atgatggcga aagagccacc gtgaaaacta 240 acgcctggtt gcggcacctt tatgacagca gaaaccctca agcgaagacc tgctccaaga 300 tcagcgggaa ttcgccaaag aagtcctacc tcagtttctt tgaggagggc agagatgagt 360 atgacagaaa gtacagctct ttcccggacg ccctcaaagt caagatgagg gccatggcgc 420 gggacatgtt ttatttcgga tatgataact acatgaagtt tgcctttcca gaggatgaac 480 tgaaccctat agcatgtgaa ggtcgagggc ctgacgtgct caatccgtca aatatcaaca 540 tcaatgatgt tcttggaaac tactctttaa ctctcatcga tactcttgat acactgttgg 600 ttctcggaaa tgtgaccgaa tttcatcgag ctgttaaatt ggtgattgat acagtatctt 660 ttgacaagga ctccactgtt caagtctttg aggccaacat tagaatcctt ggcagtttga 720 tctcagccca cattctgctg actgacccac ggcacccgtt tggagatgta ggactgaaag 780 actatgacaa tgagctgcta cacctggcac atgatcttgc cgttcgacta ctacctgctt 840 tcgagaacac tagcacaggc atcccttatc caagggtgaa tttaaagaat ggtgttcctc 900 ctgacagcat taatgagacc tgtacggcag gggcaggctc tctgctcgtg gagtttggta 960 ttcttagccg tctgattggt gactctacat ttgaatgggt cgcccggcgg gctgttaaag 1020 ctctgtggag attaagaagc aatgaaactg gactattggg gaatgtggtt aatattcaga 1080 ctggccagtg ggtgggcaag cagagcggtc tgggcgccgg catggactca ttttatgagt 1140 acctcctcaa gtcttacatc ttgtttggag agaaagaaga ctttaggatg ttcactgcag 1200 cctatgagag cattcagagc cacctgagaa gaggaagaga ggcgtgtaat gaaggagaag 1260 gtgacccacc actctatgtg aatgtgaaca tgttcaacgg acagatcatg aacacatgga 1320 ttgactctct gcaggcattc ttccctggac tacaggtttt aaacggagat gttgaaaatg 1380 ccatttgcct tcatgccttc tactatgcaa tctggaagcg tttcggtgct cttcctgaga 1440 ggtacaactg gcagcttcag gccccagatg tccttttcta ccccttaaga cctgaactag 1500 tggagtccac atacctgctc tatcaggcaa ccaagaatcc attttatctt catgttggga 1560 tggacattct tgaaagcctt gaaaaaaaat accaaagtga ggtgtggtta cgctactctt 1620 catcatgtag tggataaatc caaagaagat cgcatggaga gtttcttcct cagtgaaacc 1680 tgcaaatacc tttacttgct ctttgatgaa gacaacccac tgcacaagtc tgagaataag 1740 tacatcttca ctacagaagg ccatgtagtt ccaatagatc agcgtttcag ggagagaagt 1800 tgggaagagc agttcccaaa tgaagaggcc aggaaagacc caagagccaa cataagcaat 1860 tgtgaccgga ttcctgaaga gagacgttat ggtcttccac taaagagcat gtacatgagg 1920 caaatagacc acatggtggg gctgtcctga cgtcagtcct gcctcctgta ggtctgacat 1980 acacacaaac cccctggagg tacttaacaa ctctccaaac agaacttttc agtctcattt 2040 tgcacattga gatttttttt tatcaacaaa tcaactagca ccaattgttc tttttttgtt 2100 ccagtgtgtc tttttttgtg gcatgaaagt gtcactctta agttagatca ggtatttgga 2160 tttggttaca ttttcggatc cagccagcaa acatccacag cataaagcaa acttgttcag 2220 ggcagttcgg ctgttcagaa ttgtaacaac agttattatt attatttttt tttattcttg 2280 ggctgatttc tatattgtgc aatcgagtgt gtttacacac ccagtgttac acggtttctt 2340 cagcgccatg tttaatgaaa cttgatctag tattacaaaa gggctctgtt gggtgaacga 2400 atagtatgtt tgcatgtttc agatgttctt ctcaaggaac aagctcttaa ataagctcat 2460 gatgacagct gaatcattat acgcctttca gaaacacgca caacagaggg gctacatttt 2520 ctgcaaatct caaaaatcat caaatccaca cgttctttat cttttcatat tttgtgcctt 2580 ggtttgaaag atgtacaaca cttatatttt tgcatgattc aacatttccg attgcactga 2640 tttcattttt tttgctatca gtgcattgag ataaatggaa aggtttggtt atgtagcata 2700 catgtttggt atgttttgat ccaaattaac attgaaaacc gtatttaatt aagtgcgttt 2760 tccacagcag ctcaaacaag tgttgcaatt ttcttctctc tctctctgtt agttctggac 2820 atttatacta acaataaatc cttaaacaat atgaagtttg ttggaccaaa tattacattt 2880 aacaaaaaaa gtgccttatt catattttca ttttccttca gcgctcagga ttaatagtat 2940 gccataatgc tgtcactctg aggtgatgtg catgtaatgt gtacgtttct taactctttt 3000 gtttttatgc atatgtgttg ttagtgcaaa tgttttctga acaaaagttg acaataaggg 3060 atgatcaata tgctggtaag agaagaattc attttgttgg tgttatagat catgatttat 3120 ttcgggcaca tagggaggat actgtcagta attacttagg gggttgtatt tattcagatt 3180 gttttttatt agatgcaggt attctaattc aaattgtttc atgttgatct gcttgatttt 3240 attgttattt aacctttagt ccaaatggag acggaggttg atgtctacac tgcattttat 3300 caaatgaaag aaaaaaaata ataaaaacgt tacttaaaaa aaaaaaaaaa aa 3352 // |
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