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EBI DbfetchID BC045236; SV 1; linear; mRNA; STD; VRT; 2053 BP. XX AC BC045236; XX DT 01-FEB-2003 (Rel. 74, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Xenopus laevis tyrosyl-tRNA synthetase, mRNA (cDNA clone MGC:53284 DE IMAGE:5570143), complete cds. XX KW MGC. XX OS Xenopus laevis (African clawed frog) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia; OC Batrachia; Anura; Mesobatrachia; Pipoidea; Pipidae; Xenopodinae; Xenopus; OC Xenopus. XX RN [1] RP 1-2053 RX DOI; 10.1002/dvdy.10174 RX PUBMED; 12454917. RA Klein S.L., Strausberg R.L., Wagner L., Pontius J., Clifton S.W., RA Richardson P.; RT "Genetic and genomic tools for Xenopus research: The NIH Xenopus RT initiative"; RL Dev. Dyn. 225(4):384-391(2002). XX RN [2] RP 1-2053 RX DOI; 10.1073/pnas.242603899 RX PUBMED; 12477932. RG Mammalian Gene Collection Program Team RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., RA Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., RA Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., RA Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K., RA Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F., RA Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S., RA Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J., RA Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J., RA Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M., RA Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., RA Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., RA Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C., RA Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I., RA Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human and RT mouse cDNA sequences"; RL Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002). XX RN [3] RC NIH-MGC Project RP 1-2053 RA Klein S., Strausberg R.; RT ; RL Submitted (27-JAN-2003) to the EMBL/GenBank/DDBJ databases. RL National Institutes of Health, Xenopus Gene Collection (XGC), National RL Institute of Child Health and Human Development, 6100 Executive Boulevard, RL Room 4B01, Rockville, MD 20892-7510, USA XX DR ImaGenes; IMAGp998O0812311Q. DR ImaGenes; IRAKp961N0296Q. DR ImaGenes; IRBHp990A089D. XX CC Contact: XGC help desk CC Email: cgapbs-r@mail.nih.gov CC Tissue Procurement: Dr. Igor Dawid CC cDNA Library Preparation: Life Technologies, Inc. CC cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) CC DNA Sequencing by: Genome Sequence Centre, CC BC Cancer Agency, Vancouver, BC, Canada CC info@bcgsc.bc.ca CC Steven Jones, Jennifer Asano, Ian Bosdet, Yaron Butterfield, CC Susanna Chan, Readman Chiu, Chris Fjell, Erin Garland, Ran Guin, CC Letticia Hsiao, Martin Krzywinski, Reta Kutsche, Oliver Lee, Soo CC Sen Lee, Victor Ling, Carrie Mathewson, Candice McLeavy, Steven CC Ness, Pawan Pandoh, Anna-Liisa Prabhu, Parvaneh Saeedi, Jacqueline CC Schein, Duane Smailus, Michael Smith, Lorraine Spence, Jeff Stott, CC Michael Thorne, Miranada Tsai, Natasja van den Bosch, Jill Vardy, CC George Yang, Scott Zuyderduyn, Marco Marra. CC Clone distribution: MGC clone distribution information can be found CC through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov CC Series: IRAK Plate: 96 Row: n Column: 2 CC This clone was selected for full length sequencing because it CC passed the following selection criteria: Hexamer frequency ORF CC analysis, Similarity but not identity to protein. XX FH Key Location/Qualifiers FH FT source 1..2053 FT /organism="Xenopus laevis" FT /lab_host="DH10B" FT /mol_type="mRNA" FT /clone_lib="NICHD_XGC_Emb4" FT /clone="MGC:53284 IMAGE:5570143" FT /tissue_type="Embryo, stage 31/32, Xenopus" FT /note="Vector: pCMV-SPORT6" FT /db_xref="taxon:8355" FT gene 1..2053 FT /gene="yars-prov" FT /note="synonym: MGC53284" FT CDS 259..1845 FT /codon_start=1 FT /product="yars-prov protein" FT /db_xref="GOA:Q7ZX51" FT /db_xref="InterPro:IPR002305" FT /db_xref="InterPro:IPR002307" FT /db_xref="InterPro:IPR002547" FT /db_xref="InterPro:IPR012340" FT /db_xref="InterPro:IPR014729" FT /db_xref="InterPro:IPR015624" FT /db_xref="InterPro:IPR016027" FT /db_xref="UniProtKB/Swiss-Prot:Q7ZX51" FT /protein_id="AAH45236.1" FT /translation="MGDSLTLEGKAQLITRNLQELLGEDKMKEILKERPLRIYWGTATT FT GKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWDLLELRTRYYEQVIQAM FT LQSIGVPLERLRFIRGTEFQLSKEYTLDVYRLSSVVTQHDAKKAGAEVVKQVEHPLLSG FT LLYPGLQALDEEYLKVDAQFGGVDQRKIFTFAEKYLPALGYAKRIHLMNPMVPGLTGAK FT MSSSEEESKIDLLDSPADVKKKLKKAFCEPGNVENNGVLSFVRHVLFPLKSEFVVLRDE FT KFGGNKTYTDFETLEKDFAEELVHPGDLKASVEKALNKLLHPIREKFNSPEMKKLSNDA FT YPDASKQKSVPKGSTKNSGTEEIDPSLLDLRVGKILSVSQHPDADSLYVESVDVGEANP FT RCVVSGLVQYVPSDQLLGRSVVLLCNLKPQKMRGIESQGMLLCASTEGEQKQVEPLDPP FT SGSAPGERIYIEGYENGEPEGELKPKKKVFEKLQVDFRISDDLCAQWKGKNFLTKLGSV FT TCKTLRGGSIG" FT misc_feature 349..1221 FT /note="tRNA-synt_1b; Region: tRNA synthetases class I (W FT and Y)" FT misc_feature 1366..1656 FT /note="tRNA_bind; Region: Putative tRNA binding domain. FT This domain is found in prokaryotic methionyl-tRNA FT synthetases, prokaryotic phenylalanyl tRNA synthetases the FT yeast GU4 nucleic-binding protein (G4p1 or p42, ARC1), FT human tyrosyl-tRNA synthetase, and endothelial-monocyte FT activating polypeptide II. G4p1 binds specifically to tRNA FT form a complex with methionyl-tRNA synthetases. In human FT tyrosyl-tRNA synthetase this domain may direct tRNA to the FT active site of the enzyme. This domain may perform a common FT function in tRNA aminoacylation" XX SQ Sequence 2053 BP; 601 A; 422 C; 509 G; 521 T; 0 other; ctagccgcta ccgctaccga taactattgg gccatcatta gttcctttaa gatcgattcg 60 ctctcgctcc ttcgctgtac tgatctaagc agcgggcaat gcactcgcag ccatagctgt 120 agattgccga gcacattgag tggaatgttt ctggcttctg actgacgcaa tcagtggcaa 180 caaggaagca aatctactgc agcaacacgt gtaccggatt tgtctctgtg atttaataca 240 catatccagc aaggcaaaat gggtgacagc ttaacacttg aagggaaggc acagctcatc 300 acacggaatc ttcaggagct ccttggagag gacaaaatga aggaaatcct gaaagagaga 360 cctctgcgga tatactgggg cacagcaacc acaggaaaac cacatgtggc ttattttgtt 420 cctatgtcca agatcgcaga cttcctgaag gctggttgtg aggtgaccat cctgtttgca 480 gacctgcatg catatttaga taatatgaaa gcaccttggg atctgctgga gctcagaaca 540 agatactatg agcaagtcat ccaggctatg ctacagagca tcggagtccc gcttgagagg 600 ttacgcttta ttcggggaac agaatttcag ctcagcaaag agtacactct ggatgtatac 660 agactgtctt ctgtagttac tcagcatgat gctaagaaag ctggagctga ggtagtaaaa 720 caagtggaac acccgctgct cagtgggctt ctgtacccag ggttgcaggc tctggatgag 780 gaatacttga aggtagacgc ccagtttgga ggagttgacc aaaggaaaat cttcacattt 840 gcagaaaagt accttccggc tcttggttat gcaaaacgta ttcaccttat gaatcctatg 900 gtgccaggac tcacaggggc taaaatgagc tcctctgagg aggaatccaa aattgatctc 960 ctggactctc ctgcagatgt gaaaaaaaag ttaaagaagg cgttctgtga gcctgggaat 1020 gtagaaaaca atggggtttt aagctttgtc agacatgtcc tctttcctct aaaatcggaa 1080 tttgtggttc ttcgggatga gaaatttgga ggaaataaaa cttacactga ttttgaaaca 1140 ctggaaaaag actttgctga agagctggtt catcccggtg atctgaaggc ctcagttgag 1200 aaagctctga ataagctatt gcaccctatc agagaaaagt ttaatagccc tgaaatgaag 1260 aaattgagta atgatgcata ccctgatgcc agcaaacaga agtctgtgcc taaaggatct 1320 actaaaaact ctggaactga ggagatcgat ccttctcttc tagatttgcg agttgggaag 1380 attctcagtg tgagccagca cccagatgca gacagcctgt atgtggagtc agtggatgta 1440 ggtgaggcga atccacgctg tgtagtcagc gggctggtgc aatatgttcc ttctgaccaa 1500 ctgctgggcc gctcagtggt tttgctgtgc aacttaaagc cacagaagat gagaggaatc 1560 gagtcacagg gcatgctgct ttgcgcatcc acggaagggg aacaaaaaca agtggaacca 1620 cttgaccctc cttcaggttc tgctcctggt gagcgcatct atattgaggg atatgagaat 1680 ggagagccag agggagagct caaacctaag aagaaagtat ttgagaagtt gcaggttgac 1740 ttcaggattt cagatgattt atgcgctcaa tggaaaggga agaattttct gaccaaactg 1800 gggtctgtaa cctgtaaaac tctgcgaggc ggtagcattg gttgagcatg tttttttttt 1860 ttatcatgct ccgattcatc ttcactacca aaacagaatc acattgctgg atcccagaca 1920 acaaggggtt tatcgtccca ctatttatat aatcttcggt tgcacgtgac ctgctctagg 1980 gtcaacgtag aggtgaaaaa attgtaattc tggtatgaaa taaagttgta taccatcaaa 2040 aaaaaaaaaa aaa 2053 // |
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