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EBI DbfetchID BC044703; SV 1; linear; mRNA; STD; VRT; 3404 BP. XX AC BC044703; XX DT 24-JAN-2003 (Rel. 74, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Xenopus laevis exostoses (multiple) 2, mRNA (cDNA clone MGC:53381 DE IMAGE:5571351), complete cds. XX KW MGC. XX OS Xenopus laevis (African clawed frog) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia; OC Batrachia; Anura; Mesobatrachia; Pipoidea; Pipidae; Xenopodinae; Xenopus; OC Xenopus. XX RN [1] RP 1-3404 RX DOI; 10.1002/dvdy.10174 RX PUBMED; 12454917. RA Klein S.L., Strausberg R.L., Wagner L., Pontius J., Clifton S.W., RA Richardson P.; RT "Genetic and genomic tools for Xenopus research: The NIH Xenopus RT initiative"; RL Dev. Dyn. 225(4):384-391(2002). XX RN [2] RP 1-3404 RX DOI; 10.1073/pnas.242603899 RX PUBMED; 12477932. RG Mammalian Gene Collection Program Team RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., RA Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., RA Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., RA Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K., RA Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F., RA Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S., RA Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J., RA Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J., RA Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M., RA Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., RA Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., RA Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C., RA Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I., RA Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human and RT mouse cDNA sequences"; RL Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002). XX RN [3] RC NIH-MGC Project RP 1-3404 RA Klein S., Strausberg R.; RT ; RL Submitted (17-JAN-2003) to the EMBL/GenBank/DDBJ databases. RL National Institutes of Health, Xenopus Gene Collection (XGC), National RL Institute of Child Health and Human Development, 6100 Executive Boulevard, RL Room 4B01, Rockville, MD 20892-7510, USA XX DR ImaGenes; IMAGp998A1612315Q. DR ImaGenes; IRAKp961J0497Q. DR ImaGenes; IRBHp990D0910D. XX CC Contact: XGC help desk CC Email: cgapbs-r@mail.nih.gov CC Tissue Procurement: Dr. Igor Dawid CC cDNA Library Preparation: Life Technologies, Inc. CC cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) CC DNA Sequencing by: National Institutes of Health Intramural CC Sequencing Center (NISC), CC Gaithersburg, Maryland; CC Web site: http://www.nisc.nih.gov/ CC Contact: nisc_mgc@nhgri.nih.gov CC Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B., CC Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S., CC Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P., CC Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R., CC Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C., CC McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W., CC Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L., CC Young,A., Zhang,L.-H. and Green,E.D. CC Clone distribution: MGC clone distribution information can be found CC through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov CC Series: IRAK Plate: 97 Row: j Column: 4 CC This clone was selected for full length sequencing because it CC passed the following selection criteria: Hexamer frequency ORF CC analysis, Similarity but not identity to protein. XX FH Key Location/Qualifiers FH FT source 1..3404 FT /organism="Xenopus laevis" FT /lab_host="DH10B" FT /mol_type="mRNA" FT /clone_lib="NICHD_XGC_Emb4" FT /clone="MGC:53381 IMAGE:5571351" FT /tissue_type="Embryo, stage 31/32, Xenopus" FT /note="Vector: pCMV-SPORT6" FT /db_xref="taxon:8355" FT gene 1..3404 FT /gene="ext2-prov" FT /note="synonym: MGC53381" FT CDS 82..2238 FT /codon_start=1 FT /product="ext2-prov protein" FT /db_xref="GOA:Q7ZTM7" FT /db_xref="HSSP:1OMX" FT /db_xref="InterPro:IPR004263" FT /db_xref="InterPro:IPR015338" FT /db_xref="UniProtKB/TrEMBL:Q7ZTM7" FT /protein_id="AAH44703.1" FT /translation="MCASVKYSTRGPVLIPRMKSKHRVYYIALFSVVLLGLIATGMFQF FT WPHTIETSSDWTVDKRSVHDVPLVRLSSDCPIPERGDLSCRMHTCFDVYRCGYNPKNKV FT KVYIYPLQKYTDEYSVTVGGTISREYNQLLSAVSQSDFYTEDVSRACLFLPSIDVLNQD FT SLRIKETAQAMAQLTRWDRGTNHLLFNMLPGGPPDYNTALDVPRDRALLAGGGFSTWTY FT RQGYDVSIPVYSSLSAEVNLPEKAPGTRTHFLLSSQTSLHPEFRSELEAIRAENGESVL FT ILEKCSNYSDGAAALRKRCYKNTVYDYPQILQESTFCIVLRGARLGQGLLSDVLQAGCV FT PVIIADSYVLPFSEVLDWKRASVVIPEEKMFEMYSILQGIPQRQVEEMQRQARWFWEGY FT FSSMKSIALATLQIINDRIYPYAARSYEEWNDPPSVQRSSVNNPLFLPIIPPQSQGFTA FT VVLTYDRVESLFRVITEISKVPSLSKLLVVWNNQNKNPPEASVWPKIRVPLKVVRTTEN FT KLSNRFFPYSEIETEAVLAIDDDIIMLTSDELQFGYEVWREFPDRLVGYPGRLHLWDHE FT MSKWKYESEWTNEVSMVLTGAAFYHKYFNYLYTYKMPGDIKNWVDAHMNCEDIAMNFLV FT ANVTGKAPIKVTPRKKFKCPECTAIDGLSLDQTHMVERSECINKFASVFGTMPLKVVEH FT RADPVLYKDDFPEKLKSFPNIGSL" FT misc_feature 397..1257 FT /note="Exostosin; Region: Exostosin family. The EXT family FT is a family of tumour suppressor genes. Mutations of EXT1 FT on 8q24.1, EXT2 on 11p11-13, and EXT3 on 19p have been FT associated with the autosomal dominant disorder known as FT hereditary multiple exostoses (HME). This is the most FT common known skeletal dysplasia. The chromosomal locations FT of other EXT genes suggest association with other forms of FT neoplasia. EXT1 and EXT2 have both been shown to encode a FT heparan sulphate polymerase with both D-glucuronyl (GlcA) FT and N-acetyl-D-glucosaminoglycan (GlcNAC) transferase FT activities. The nature of the defect in heparan sulphate FT biosynthesis in HME is unclear" XX SQ Sequence 3404 BP; 885 A; 847 C; 806 G; 866 T; 0 other; gtcgtgagac ggctggagga ggggcggccc gggagctgct tggggccaag cagatccgaa 60 tcctgggagg agggggccag tatgtgtgcc tcggtcaagt acagcacaag gggtccagtc 120 ctgatcccca ggatgaagtc caagcaccgc gtttactaca tcgccctctt ctctgtggtg 180 ctgctgggac ttattgccac gggcatgttt cagttctggc cgcacactat cgagacttcc 240 agtgattgga cggtggataa gagaagcgtt catgacgtgc cattggtcag actctcgtcg 300 gactgtccaa tcccagagag gggagactta agctgcagga tgcacacctg ctttgacgta 360 tatcgctgcg gatacaaccc caagaacaag gtgaaggtgt acatctaccc gctgcagaaa 420 tacacagacg agtacagcgt gacggtgggg ggcaccatct cccgggagta caaccagctt 480 ctcagtgccg tctcccagag cgacttctac accgaagatg tgagcagggc ctgtctcttt 540 ctgccctcca tcgatgttct caaccaagac agtctgcgta ttaaagagac ggcacaagcg 600 atggcacagc taaccagatg ggaccgaggt accaaccacc tactatttaa catgcttcct 660 ggtggacccc cagattataa cactgctctt gatgtccccc gagacagagc cttgttggcc 720 ggaggaggat tttcgacgtg gacctaccgg cagggttatg acgtcagtat cccagtgtac 780 agctccctgt ctgcagaggt gaacctacct gagaaagccc ctggcacccg aacccacttc 840 ctcctgtcct cccagacttc tttgcaccca gagttccgct cagagctgga agccatccgg 900 gcagagaatg gagaatcggt gctgatcttg gaaaagtgca gcaattattc tgacggtgcc 960 gcggctctcc gcaagcgctg ctacaagaac acggtgtacg actacccgca gatcctccag 1020 gaatccacct tctgtatagt cctgcgaggg gctcggctgg ggcaggggct tctcagtgac 1080 gtcctgcagg ctggttgtgt gcccgttatt atcgcagatt cctatgtgct tcccttctcc 1140 gaggtcctgg attggaaaag agcatcggtg gtgataccag aagagaagat gtttgagatg 1200 tacagcattc tacagggaat tcctcagcgg caagtggaag agatgcagcg tcaggctcgc 1260 tggttctggg aagggtattt cagctccatg aaatccatcg ctttggcgac ccttcagatt 1320 attaacgacc gcatctaccc ctacgctgct cgctcatacg aagagtggaa cgacccgccc 1380 tctgtgcaaa ggagcagtgt aaacaaccct ctcttcctgc cgatcatccc ccctcagtcc 1440 caggggttca ccgccgtggt gctgacgtac gaccgggtcg aaagcttgtt ccgtgtcatc 1500 accgaaatct ccaaggtccc aagtctgtcc aagctgctag tggtgtggaa taatcagaat 1560 aaaaaccctc ccgaagcttc tgtttggcca aagatccggg ttccattgaa agtcgtgagg 1620 accactgaga acaagctgag caaccgcttc ttcccttaca gtgaaattga gacagaagcc 1680 gttctggcta ttgacgatga catcatcatg ctgacatcag atgagcttca gtttggttac 1740 gaggtatggc gggagtttcc agacaggttg gtgggatacc cgggccggct gcacctgtgg 1800 gatcatgaaa tgagcaagtg gaaatatgaa tccgagtgga caaacgaggt gtcgatggtt 1860 ctgacgggtg cagctttcta ccacaagtac ttcaactacc tgtacaccta caagatgccc 1920 ggggacatca agaactgggt cgacgcccac atgaactgcg aagatatcgc catgaacttc 1980 ttagtggcca acgtgacagg caaggcacct attaaggtga ctccgagaaa gaagttcaag 2040 tgcccagaat gcacggccat cgatgggctg tcactggatc agacgcacat ggtggagaga 2100 tctgaatgta tcaacaagtt tgcgtcggtt ttcggcacca tgcccctgaa ggtggtggag 2160 caccgtgctg accccgtgct gtacaaggac gacttcccag agaagctgaa gagtttcccc 2220 aacatcggca gcctgtgaga atgtcgccga gcaaccgcat cttcactttg cggcactgct 2280 gcaccgtcac cctggcccct agtctttcca tttcacacgt tttattggtg attttaacac 2340 gtctctccta gctgctctct ctacttatta tggtcggact tgttataggg ataagataaa 2400 cctacaacgt gttttatatt aaatacctgc cggcaaccaa tcagaatcca ctgagaatat 2460 cgcagagcct ttattggaca ccgtgtcatg ggaatatata atatattcat ctatataact 2520 gctcattaca agcagaaaca caatgagctt gatatagaaa cggctgccgg tcgccatgtt 2580 tatttcccca gaattccact ttctcacata gtggtgactg tctcccacgt tagtgaccgg 2640 tctcagccaa tcatatattt gctttcactc ataagccgca gctgattggt ggagctcata 2700 ccccctacta tacactcacc cacacccgca tctttcaggg actctttctc atagatgtgc 2760 atttcgttct cttttatatt ggtcatgttt ttatattatt atgttttttt tagcatttta 2820 aatatttttt ttaaagattt ccctttcacc tatagatctt tatattattt aatgttgttt 2880 ttttttttaa tgaataggaa ctcgcagtgc taatgggggg gtagacttta cctgaattct 2940 gtaacctcaa ccattcccgt gaggtttctc taggtggtct cacctcccct gcccagttca 3000 acccccagtt aaaggggtgg ctcaccttta atttaacttt taattaggga tgcaccaaat 3060 ccaggattcg tttcagaatt ctgccttttt cagcaggatt cagtcaaatc cttctgcccg 3120 gccaaaccaa atcctaattt gcatatgcaa attaaggggt gggagggaaa tttcgtgatt 3180 tttttcctct tcccacctct aatttgcata tgcaaattcg gatttggttc gggcgaacct 3240 tttgtgaagg attttggatt tagtggattc agtgcattcc tacttttact atgttataaa 3300 ttggataatt ctaagtaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 3360 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa 3404 // |
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