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EBI DbfetchID BC043761; SV 1; linear; mRNA; STD; VRT; 3081 BP. XX AC BC043761; XX DT 14-JAN-2003 (Rel. 74, Created) DT 21-OCT-2008 (Rel. 97, Last updated, Version 6) XX DE Xenopus laevis hypothetical protein MGC52920, mRNA (cDNA clone MGC:52920 DE IMAGE:4889645), complete cds. XX KW MGC. XX OS Xenopus laevis (African clawed frog) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia; OC Batrachia; Anura; Mesobatrachia; Pipoidea; Pipidae; Xenopodinae; Xenopus; OC Xenopus. XX RN [1] RP 1-3081 RX DOI; 10.1002/dvdy.10174 RX PUBMED; 12454917. RA Klein S.L., Strausberg R.L., Wagner L., Pontius J., Clifton S.W., RA Richardson P.; RT "Genetic and genomic tools for Xenopus research: The NIH Xenopus RT initiative"; RL Dev. Dyn. 225(4):384-391(2002). XX RN [2] RP 1-3081 RX DOI; 10.1073/pnas.242603899 RX PUBMED; 12477932. RG Mammalian Gene Collection Program Team RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., RA Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., RA Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., RA Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K., RA Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F., RA Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S., RA Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J., RA Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J., RA Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M., RA Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., RA Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., RA Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C., RA Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I., RA Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human and RT mouse cDNA sequences"; RL Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002). XX RN [3] RC NIH-MGC Project RP 1-3081 RA Klein S., Strausberg R.; RT ; RL Submitted (10-JAN-2003) to the EMBL/GenBank/DDBJ databases. RL National Institutes of Health, Xenopus Gene Collection (XGC), National RL Institute of Child Health and Human Development, 6100 Executive Boulevard, RL Room 4B01, Rockville, MD 20892-7510, USA XX DR ImaGenes; IMAGp998M0610808Q. DR ImaGenes; IRAKp961E1095Q. DR ImaGenes; IRBHp990E014D. DR ImaGenes; RZPDp988G0373D. XX CC Contact: XGC help desk CC Email: cgapbs-r@mail.nih.gov CC Tissue Procurement: Dr. Igor Dawid CC cDNA Library Preparation: Life Technologies, Inc. CC cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) CC DNA Sequencing by: Genome Sequence Centre, CC BC Cancer Agency, Vancouver, BC, Canada CC info@bcgsc.bc.ca CC Steven Jones, Jennifer Asano, Ian Bosdet, Yaron Butterfield, CC Susanna Chan, Readman Chiu, Chris Fjell, Erin Garland, Ran Guin, CC Letticia Hsiao, Martin Krzywinski, Reta Kutsche, Oliver Lee, Soo CC Sen Lee, Victor Ling, Carrie Mathewson, Candice McLeavy, Steven CC Ness, Pawan Pandoh, Anna-Liisa Prabhu, Parvaneh Saeedi, Jacqueline CC Schein, Duane Smailus, Michael Smith, Lorraine Spence, Jeff Stott, CC Michael Thorne, Miranada Tsai, Natasja van den Bosch, Jill Vardy, CC George Yang, Scott Zuyderduyn, Marco Marra. CC Clone distribution: MGC clone distribution information can be found CC through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov CC Series: IRAK Plate: 95 Row: e Column: 10 CC This clone was selected for full length sequencing because it CC passed the following selection criteria: Hexamer frequency ORF CC analysis, Similarity but not identity to protein. XX FH Key Location/Qualifiers FH FT source 1..3081 FT /organism="Xenopus laevis" FT /lab_host="DH10B" FT /mol_type="mRNA" FT /clone_lib="NICHD_XGC_Emb2" FT /clone="MGC:52920 IMAGE:4889645" FT /tissue_type="Embryo, stage 17/19, Xenopus" FT /note="Vector: pCMV-SPORT6" FT /db_xref="taxon:8355" FT gene 1..3081 FT /gene="MGC52920" FT CDS 72..1358 FT /codon_start=1 FT /product="MGC52920 protein" FT /db_xref="GOA:Q7ZYJ3" FT /db_xref="InterPro:IPR001206" FT /db_xref="UniProtKB/Swiss-Prot:Q7ZYJ3" FT /protein_id="AAH43761.1" FT /translation="MARVVRIFKTLRNHWKKSTVGFCLLAYGSHWLYGKHCDNLLRRAA FT CEEAQVFGNHQILPHSAIKKATVFLNPAACKGKARTLFEKNAAPVLHLAGIDITVVKTD FT YEGQAKKLLELMEKTDLIIVAGGDGTVQEVITGLLRRDDEASFSKIPIGFIPLGGTNTL FT SHTLYPERENKVEQITEATLSILKGETVPLDVLQIKGEQDQPVFAVQGIRWGSYRDASV FT KVSKYWYLGPLKARAAHLFSALKEWPQQHQASISYLGPAERPPEEPEQKPSRPPLYVRI FT YRRLALYWSPPKVEVPVEPTPEPWEEAQLSAVELSITTQNHQPDLLRTLDSMSIHIEPD FT TISKGKFIQLGAQKMTDPLLHPEDSQVLLASRCSLHLPQGTEGHFSIDSEEYEAMSVDV FT TLLPRKLHFLCHPTRKQELLQSPTATAQS" FT misc_feature 273..653 FT /note="DAGKc; Region: Diacylglycerol kinase catalytic FT domain (presumed). Diacylglycerol (DAG) is a second FT messenger that acts as a protein kinase C activator. The FT catalytic domain is assumed from the finding of bacterial FT homologues" XX SQ Sequence 3081 BP; 1024 A; 805 C; 660 G; 592 T; 0 other; ggagaaacaa cgtgcgttta tagggagggc ggaagcacac agcagctttc tgcaaacttt 60 ggactccaac aatggcgcgg gtggtacgga tctttaaaac tctccggaac cactggaaga 120 aaagcactgt gggattttgt ttgttggctt atggaagcca ttggctgtat ggcaaacact 180 gtgataacct actgagaaga gccgcctgtg aagaagcgca ggtctttgga aaccaccaga 240 tcctccccca ttcggcgatc aagaaagcaa cggtatttct aaatccggca gcatgtaaag 300 gcaaagcgag gaccctcttt gagaagaatg cagcccccgt cctacattta gccggcatag 360 atatcactgt tgtaaagaca gattatgaag gtcaagctaa gaagctgctg gaactcatgg 420 aaaagacaga tctgatcatt gttgctggag gagatggaac tgtacaagag gttatcacgg 480 ggctcctgcg cagggacgat gaggcctcct tcagtaaaat ccccatcgga ttcatcccac 540 tgggagggac caacacactt agccacaccc tctatcctga aagggagaat aaggtcgagc 600 agatcacaga ggccacactt tccatactaa aaggagagac tgttcccctt gatgtccttc 660 agataaaggg ggagcaagat cagcctgtgt ttgcagtgca gggaatacga tggggcagct 720 accgggatgc ttcagtgaaa gtcagcaagt attggtatct tgggccactg aaggccagag 780 cagctcatct gttcagtgca ctaaaggagt ggccccagca acatcaggcc tccatctcat 840 acctcggacc tgctgaacga cccccggagg agccggagca gaaaccaagt cgcccccctc 900 tctatgtccg aatatatagg agactggctc tttactggtc acctcctaaa gttgaggtcc 960 ctgttgaacc caccccagaa ccctgggagg aagcccagct ctctgctgtt gaactttcta 1020 tcaccaccca gaaccatcag cctgacctcc tgcgcacttt ggattccatg agtattcata 1080 tagaacctga caccatctct aaagggaaat ttatccagtt gggtgctcag aagatgactg 1140 accctctact gcaccctgag gactcccagg ttctacttgc cagtcgctgc agcctgcacc 1200 tcccccaggg aacggagggg catttcagca tcgattctga ggagtatgaa gccatgtccg 1260 tggatgtgac ccttttgccc cgcaagctgc attttctgtg ccatcccaca cggaaacaag 1320 agcttctgca gagcccaaca gcaactgccc aaagctagcg ccaccctgtg ataaagcatt 1380 atcaaccaat gcaaaaacta cagcgggaat gtagcgccac catgtggctg aaggcaatgg 1440 ttttacccaa cacaagtcag aaacaagagc caatgattct acccaacaca agtcagaaac 1500 aagagccaat gattctaccc aacacaagtc agaaacaagg gccaatggtt ctacccaaca 1560 caagtcagaa acaagggcca atggttttac ccaacacaag tcagaaacaa gggccaatgg 1620 ttctacccaa cacaagtcag aaacaagagc caatgattct acccaacaca agtcagaaac 1680 aagggccaat gattctaccc aacacaagtc agaaacaagg gccaatggtt ctacccaaca 1740 caagtcagaa acaagggcca atgattctac ccaacacaaa ttagaaacaa gggtcaatgg 1800 ttctacccaa cgcaagttag aaacaagggc caatgattct acccaatgca agtcagaaac 1860 aagggccaat ggttctaccc aacacaagtc agaaaaaagg gccaatggtt ctacccaaca 1920 caagtcagaa acaagagcca atggttctac ccaacacaag tcagaaacaa gagccaatga 1980 ttctacccaa cacaagtcag aaacaagggc caatgattct acccaacaca agtcagaaac 2040 aagggccaat ggttctaccc aacacaagtc agaaacaagg gccaatggtt ctacccaaca 2100 caagtcagaa acaagagcca atggttctac ccaacacaag tcagaaacaa gggccaatgg 2160 ttctacccaa cacaagtcag aaacaagggc caatggttct acccaacaca agtcagaaac 2220 aagggccaat ggttctaccc aacacaagtc agaaacaagg gccaatggtt ctacccaaca 2280 caagtcagaa acaagagcca atgattctac ccaacacaag tcagaaacaa gggccaatga 2340 ttctacccaa cacaagtcag aaacaagggc caatggttct acccaacaca agtcagaaac 2400 aagggccaat gattctaccc aacacaaatt agaaacaagg gccaatgatt ctacccaaca 2460 caaattagaa acaagggtca atggttctac ccaacgcaag ttagaaacaa gggccaatga 2520 ttctacccaa cgcaagtcag aaacaagggc caatggttct acccaacaca agttagaaac 2580 aagggtcaat ggttctaccc aacgcaagtc agaaagatgg gccaatggtt ctacccaaca 2640 caagtcagaa acaagggcca atggttctat ccaacacaag tcagaaacaa ggggcaatat 2700 cactgccatg gtcacttgct cgtccgtcct actgccagct ctacagagac attatagttc 2760 tgctctgctt ctcacttacg ctgcatggaa cctgctgcac cagcaacact gtatgagagg 2820 tactggaaca ccagttgggt tagttctaca gaacagagcc tatccctcat ttgactgaac 2880 tggagatcag gaacagcggg tccaaacaac ccagcctgga gtgagaatcc aatactccca 2940 ctccagggtt tgttttcgga cacctgataa gacagaataa tgaataaatg taagtgcttt 3000 taactttgca gtcaccatta tatgtgaaca tgtgttaaac gaatatatac cctttaaaaa 3060 taaaaaaaaa aaaaaaaaaa a 3081 // |
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