EBI Dbfetch

ID   BC041194; SV 1; linear; mRNA; STD; VRT; 1381 BP.
XX
AC   BC041194;
XX
DT   27-DEC-2002 (Rel. 74, Created)
DT   21-OCT-2008 (Rel. 97, Last updated, Version 6)
XX
DE   Xenopus laevis hydroxysteroid (17-beta) dehydrogenase 12, mRNA (cDNA clone
DE   MGC:52629 IMAGE:4681332), complete cds.
XX
KW   MGC.
XX
OS   Xenopus laevis (African clawed frog)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Mesobatrachia; Pipoidea; Pipidae; Xenopodinae; Xenopus;
OC   Xenopus.
XX
RN   [1]
RP   1-1381
RX   DOI; 10.1002/dvdy.10174
RX   PUBMED; 12454917.
RA   Klein S.L., Strausberg R.L., Wagner L., Pontius J., Clifton S.W.,
RA   Richardson P.;
RT   "Genetic and genomic tools for Xenopus research: The NIH Xenopus
RT   initiative";
RL   Dev. Dyn. 225(4):384-391(2002).
XX
RN   [2]
RP   1-1381
RX   DOI; 10.1073/pnas.242603899
RX   PUBMED; 12477932.
RG   Mammalian Gene Collection Program Team
RA   Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D.,
RA   Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F.,
RA   Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H.,
RA   Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K.,
RA   Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F.,
RA   Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S.,
RA   Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J.,
RA   Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J.,
RA   Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M.,
RA   Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X.,
RA   Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA   Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C.,
RA   Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I.,
RA   Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.;
RT   "Generation and initial analysis of more than 15,000 full-length human and
RT   mouse cDNA sequences";
RL   Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002).
XX
RN   [3]
RC   NIH-MGC Project
RP   1-1381
RA   Klein S., Strausberg R.;
RT   ;
RL   Submitted (16-DEC-2002) to the EMBL/GenBank/DDBJ databases.
RL   National Institutes of Health, Xenopus Gene Collection (XGC), National
RL   Institute of Child Health and Human Development, 6100 Executive Boulevard,
RL   Room 4B01, Rockville, MD 20892-7510, USA
XX
DR   ImaGenes; IMAGp998E1310561Q.
DR   ImaGenes; IRAKp961B2394Q.
DR   ImaGenes; IRBHp990H041D.
XX
CC   Contact: XGC help desk
CC   Email: cgapbs-r@mail.nih.gov
CC   Tissue Procurement: Dr. Igor Dawid
CC   cDNA Library Preparation: Life Technologies, Inc.
CC   cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
CC   DNA Sequencing by: Institute for Systems Biology
CC   http://www.systemsbiology.org
CC   contact: amadan@systemsbiology.org
CC   Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
CC   Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
CC   Clone distribution: MGC clone distribution information can be found
CC   through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
CC   Series: IRAK Plate: 94 Row: b Column: 23
CC   This clone was selected for full length sequencing because it
CC   passed the following selection criteria: Hexamer frequency ORF
CC   analysis, Similarity but not identity to protein.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1381
FT                   /organism="Xenopus laevis"
FT                   /lab_host="DH10B"
FT                   /mol_type="mRNA"
FT                   /clone_lib="NICHD_XGC_Emb4"
FT                   /clone="MGC:52629 IMAGE:4681332"
FT                   /tissue_type="Embryo, stage 31/32, Xenopus"
FT                   /note="Vector: pCMV-SPORT6"
FT                   /db_xref="taxon:8355"
FT   gene            1..1381
FT                   /gene="let-767co-prov"
FT                   /note="synonym: MGC52629"
FT   CDS             93..1049
FT                   /codon_start=1
FT                   /product="let-767co-prov protein"
FT                   /db_xref="GOA:Q8AVY8"
FT                   /db_xref="InterPro:IPR016040"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q8AVY8"
FT                   /protein_id="AAH41194.1"
FT                   /translation="MAPESLAEVPGCNCFWYLGVVAATWWGLRAAWCLLNGARVWVLGS
FT                   GAQVGPTIGKWAVVTGATDGIGKAYAEELARRGMNIVLISRSPEKLEEAAIHIKQKFKV
FT                   ETKIIAADFGKPTEIYERIEAGLRDLEIGVLVNNVGISYEYPEYFLEIPDLENTLDKMI
FT                   NINIMSVCQMTRLVLPGMLGRGKGVVLNISSASGMYPVPLLTVYSATKAFVDFFSRGLH
FT                   AEYRSKGVTVQSVLPFFVATKLAKIRKPTWDKPSPETYVRSALNTVGLQTQTNGYLPHA
FT                   ITGWISTSLVPVSAAISMGMKMNKGLRARFLKKAKQN"
FT   misc_feature    252..866
FT                   /note="adh_short; Region: short chain dehydrogenase. This
FT                   family contains a wide variety of dehydrogenases"
XX
SQ   Sequence 1381 BP; 351 A; 360 C; 358 G; 312 T; 0 other;
     ccggccttgt tcttgtcttc gcacacggtg ttcagctgct cttccgggtg ctgttccatt        60
     tagcccaaca attcccgtgt ttcccgtgag acatggcccc ggagtccctg gcggaggtgc       120
     ctggctgtaa ttgcttctgg tacttgggtg tggtagcggc cacctggtgg ggtctgaggg       180
     ctgcatggtg cttgctgaac ggtgcccggg tctgggtgct ggggagtgga gcgcaggtgg       240
     gccccacgat cgggaaatgg gcagttgtta ctggtgccac tgacggaatc ggaaaagcct       300
     atgccgaaga gctcgcaagg cggggaatga acatcgttct gatcagccga tctccagaaa       360
     aactagaaga ggctgccatt cacataaaac agaaattcaa ggtggagacg aagatcattg       420
     cggctgattt tggaaaaccc acggaaatct atgagcggat tgaagcaggc ctgcgagatc       480
     tggagattgg cgtcctggtt aacaacgttg gcatatctta tgaataccca gagtattttc       540
     ttgaaatccc agacctggaa aataccctgg acaaaatgat caacattaac atcatgtctg       600
     tatgtcagat gacacgattg gtgctgccgg gcatgctggg aaggggcaaa ggagtcgtac       660
     tgaacatctc ctcggccagc ggcatgtacc ctgtcccctt gctcacagtg tattctgcca       720
     ccaaagcgtt tgtggatttc ttctcccggg ggctccatgc agaatacaga agcaagggtg       780
     tcaccgtgca gagcgttctc ccattctttg tcgccaccaa actggccaag atccgtaagc       840
     caacctggga caagccaagc ccggaaacct acgtccgatc ggctctgaat actgtcggct       900
     tacagacgca gaccaacggc tacctgcccc acgccatcac gggctggatc agcacctctt       960
     tagtgccggt ctctgccgca atcagcatgg gcatgaagat gaacaagggt ctccgcgctc      1020
     gcttcctgaa gaaggccaaa caaaactaag aggagctgtt gccaggagca gccgcggccc      1080
     catagtggta ctcggcgtgt gcacccaaga gagtgtgtca ccaaaatcaa ccaaaggaac      1140
     ccacttagtt tgcctttgat ctctgaccag gaatatgccg ccaagtatgc tgtggcagct      1200
     caacctaaac tagtctacta accaacctat gcacaactaa ctagttcata tgattagact      1260
     ctaggacagg actgatcatg tgatctgcac tatagactgt tctactgccc tgtcttcctg      1320
     ctccccaaca actgatgaac tcttgattag taataaatca ataacaaaaa aaaaaaaaaa      1380
     a                                                                      1381
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