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EBI DbfetchID BC041180; SV 1; linear; mRNA; STD; VRT; 2243 BP. XX AC BC041180; XX DT 15-JAN-2003 (Rel. 74, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Xenopus laevis similar to mannoside acetylglucosaminyltransferase 1, mRNA DE (cDNA clone MGC:52514 IMAGE:4885316), complete cds. XX KW MGC. XX OS Xenopus laevis (African clawed frog) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia; OC Batrachia; Anura; Mesobatrachia; Pipoidea; Pipidae; Xenopodinae; Xenopus; OC Xenopus. XX RN [1] RP 1-2243 RX DOI; 10.1002/dvdy.10174 RX PUBMED; 12454917. RA Klein S.L., Strausberg R.L., Wagner L., Pontius J., Clifton S.W., RA Richardson P.; RT "Genetic and genomic tools for Xenopus research: The NIH Xenopus RT initiative"; RL Dev. Dyn. 225(4):384-391(2002). XX RN [2] RP 1-2243 RX DOI; 10.1073/pnas.242603899 RX PUBMED; 12477932. RG Mammalian Gene Collection Program Team RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., RA Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., RA Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., RA Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K., RA Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F., RA Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S., RA Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J., RA Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J., RA Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M., RA Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., RA Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., RA Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C., RA Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I., RA Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human and RT mouse cDNA sequences"; RL Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002). XX RN [3] RC NIH-MGC Project RP 1-2243 RA Klein S., Strausberg R.; RT ; RL Submitted (16-DEC-2002) to the EMBL/GenBank/DDBJ databases. RL National Institutes of Health, Xenopus Gene Collection (XGC), National RL Institute of Child Health and Human Development, 6100 Executive Boulevard, RL Room 4B01, Rockville, MD 20892-7510, USA XX DR ImaGenes; IMAGp998H2110797Q. DR ImaGenes; IRAKp961O2394Q. DR ImaGenes; IRBHp990D103D. DR ImaGenes; RZPDp988D0570D. XX CC Contact: XGC help desk CC Email: cgapbs-r@mail.nih.gov CC Tissue Procurement: Dr. Igor Dawid CC cDNA Library Preparation: Life Technologies, Inc. CC cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) CC DNA Sequencing by: Institute for Systems Biology CC http://www.systemsbiology.org CC contact: amadan@systemsbiology.org CC Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha CC Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting CC Clone distribution: MGC clone distribution information can be found CC through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov CC Series: IRAK Plate: 94 Row: o Column: 23 CC This clone was selected for full length sequencing because it CC passed the following selection criteria: matched mRNA gi: 15211611. XX FH Key Location/Qualifiers FH FT source 1..2243 FT /organism="Xenopus laevis" FT /lab_host="DH10B" FT /mol_type="mRNA" FT /clone_lib="NICHD_XGC_Emb2" FT /clone="MGC:52514 IMAGE:4885316" FT /tissue_type="Embryo, stage 17/19, Xenopus" FT /note="Vector: pCMV-SPORT6" FT /db_xref="taxon:8355" FT gene 1..2243 FT /gene="MGC52514" FT CDS 563..1897 FT /codon_start=1 FT /product="MGC52514 protein" FT /db_xref="GOA:Q7ZYV0" FT /db_xref="HSSP:1FO8" FT /db_xref="InterPro:IPR004139" FT /db_xref="UniProtKB/TrEMBL:Q7ZYV0" FT /protein_id="AAH41180.1" FT /translation="MPRKVSVAAWGAALFISWNAILLLYLMSRSRGTDHSDLTAHVIQL FT AEEAEAELEKQKGLLQQIHYYSGLLNHQQPPSHVRPEPITPNMSFPSPSPVGSGPLPLV FT IPILVVACDRPSVRRCLDSLLKYRPSAENFPIIVSQDCGHEETSKIIDSYGDAVTHIKQ FT PDLSEVAVPPEHRKFQGYYKISRHYRWALNQIFKTMGYKAAIVVEDDLEVAPDFYEYFQ FT TTISLLQKDRMLWCVSAWNDNGKEALIDPGGSSLLYRSDFFPGLGWLLLLELWEELEPK FT WPSAFWDDWVRRPEQRLGRACVRPELSRTRTFGRKGVSQGQFFDQHLRFIKLNQDPVAF FT TKMDLSYLLKDTYDPWFRKQVYGAPKARAEEVLHGQVPGGRTVRVEYTTKDTFKAMARA FT FGVMEDLKSGVARAAYKGVVSFTHRGRRVFLAPPKDWAGYDPSWT" FT misc_feature 590..1891 FT /note="GNT-I; Region: GNT-I family. FT Alpha-1,3-mannosyl-glycoprotein FT beta-1,2-N-acetylglucosaminyltransferase (GNT-I, GLCNAC-T FT I) EC:2.4.1.101 transfers N-acetyl-D-glucosamine from UDP FT to high-mannose glycoprotein N-oligosaccharide. This is an FT essential step in the synthesis of complex or hybrid-type FT N-linked oligosaccharides. The enzyme is an integral FT membrane protein localised to the Golgi apparatus, and is FT probably distributed in all tissues. The catalytic domain FT is located at the C-terminus" XX SQ Sequence 2243 BP; 665 A; 460 C; 538 G; 580 T; 0 other; atggatatgg tgtggacgga gggaggaaga gcgaagaaat tgcagaaatc catcgattaa 60 cacgtgacct cgaggtctgg catgtgaagt aagaatgagg tcatacagaa aactgggaag 120 gtggagaaca gaattaactg acttcccgag aagcaaagca ggaaggagag gggtagtcca 180 actcatttgt gaaggaagca ctgattacag aggaagtata aaaacacatt tctaggaaag 240 gaaagagacg acaaaaaaag tgcagagaat aagcatagtt tgaaatgtgc tagctgtgag 300 atcggcaaaa gaagactaga agtcacttgg agagcaacag gtgcctagat gtaattttta 360 ttccaggaga aaacatattt atctgctaat cctgctggat gatccactta atggccacgg 420 tggtctcttg ctttggcctg catctcttta attgaaaaaa acaagaacct tgacctgttt 480 tcttttcagc ttcagaagtg caatacttaa gtctctaaac ccccaatcac tttccatact 540 ggatcaggga ccccccaggg gaatgccgcg caaggtcagc gtggcggcct ggggggcagc 600 cctctttatc tcatggaatg ctattctact cctctacctc atgagcaggt ctcgtggcac 660 tgatcattct gatcttactg ctcatgttat ccagttggct gaagaagcag aagctgaact 720 tgaaaaacag aagggattac ttcaacaaat acactattat agtggcctac ttaaccatca 780 gcagcctcct tcacatgtaa ggcctgaacc tataacacct aatatgtcat tcccttctcc 840 atctcctgtg ggatctggtc ctctcccttt ggttattcca atactagtgg tggcatgtga 900 ccgcccctct gtgaggagat gcctggattc attgttaaaa taccgaccct cggcagaaaa 960 ttttcccatt atagtgagcc aagactgtgg tcatgaggag acttcaaaga tcatagactc 1020 ctatggagat gccgtaactc atatcaaaca acctgatctc tcggaggtgg ctgtcccacc 1080 agaacaccgg aagtttcaag gctattataa aatttcccgc cattacaggt gggcactcaa 1140 ccagattttc aaaaccatgg gttacaaagc agccattgtg gtcgaagatg acctggaggt 1200 ggctccagat ttttatgagt attttcagac aactatctcc cttctgcaaa aggaccgtat 1260 gctatggtgt gtctcagcct ggaatgacaa tggcaaggaa gctttgattg atcctggggg 1320 tagttccctt ctttaccgct cagacttctt tcctggactt ggctggttgc tactcctgga 1380 gctgtgggag gagcttgaac ctaaatggcc ctcagcattc tgggatgact gggttaggcg 1440 tcctgaacaa aggttgggta gagcttgtgt aagaccagag ctgtctcgca cacggacctt 1500 tgggagaaaa ggtgttagcc aaggacaatt ttttgatcag cacctacgat tcatcaaact 1560 taatcaggac ccagtggcct tcactaagat ggacctctcc taccttttga aagatactta 1620 tgatccttgg ttccgtaagc aggtttatgg ggcacccaaa gcacgggcag aggaggtgct 1680 acacgggcaa gtgcccgggg gcaggacggt aagagtggag tatacaacta aagacacttt 1740 taaggctatg gccagggcct ttggcgtgat ggaggactta aagtctgggg tggcgagagc 1800 ggcttacaaa ggagttgtgt cctttactca cagagggaga agggtgttct tggctcctcc 1860 caaagactgg gcaggctatg acccatcgtg gacctaaatg gatctctttt ttccaaatag 1920 tggggacgag actttatttt aataagcaaa gcaagggtaa ttatatggag aaataaggag 1980 taattatggc ttttatacaa aagaaatttt gttccaatgg tgctgtattg tctaaaaaca 2040 gagataagca ctcaaaacaa aaatgataaa gtaatagagc ttttctttgt aagagatctt 2100 aggacaccaa aatatgtgct agcaacattg aatgatttca gccaaatttt tttttgcagc 2160 agaaggcttt tccccgtttt aatggtattg cctaaataaa gtgaccttta ttgccaaaaa 2220 aaaaaaaaaa aaaaaaaaaa aaa 2243 // |
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