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EBI DbfetchID BC031752; SV 1; linear; mRNA; STD; MUS; 2988 BP. XX AC BC031752; XX DT 10-SEP-2002 (Rel. 73, Created) DT 24-SEP-2008 (Rel. 97, Last updated, Version 10) XX DE Mus musculus mannoside acetylglucosaminyltransferase 1, mRNA (cDNA clone DE MGC:25377 IMAGE:4483519), complete cds. XX KW MGC. XX OS Mus musculus (house mouse) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; OC Muridae; Murinae; Mus. XX RN [1] RP 1-2988 RX DOI; 10.1073/pnas.242603899 RX PUBMED; 12477932. RG Mammalian Gene Collection Program Team RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., RA Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., RA Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., RA Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K., RA Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F., RA Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S., RA Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J., RA Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J., RA Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M., RA Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., RA Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., RA Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C., RA Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I., RA Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human and RT mouse cDNA sequences"; RL Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002). XX RN [2] RC NIH-MGC Project URL: http://mgc.nci.nih.gov RP 1-2988 RG NIH MGC Project RA ; RT ; RL Submitted (06-JUN-2002) to the EMBL/GenBank/DDBJ databases. RL National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda, RL MD 20892-2590, USA XX DR ASTD; TRAN00000159335. DR Ensembl-Gn; ENSMUSG00000020346; Mus_musculus. DR Ensembl-Tr; ENSMUST00000081794; Mus_musculus. DR ImaGenes; IMAGp998C0810322Q. DR ImaGenes; IRAKp961I2231Q. DR ImaGenes; IRAVp968D1151D. XX CC Contact: MGC help desk CC Email: cgapbs-r@mail.nih.gov CC Tissue Procurement: Gilbert Smith, Ph.D. CC cDNA Library Preparation: Life Technologies, Inc. CC cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) CC DNA Sequencing by: Baylor College of Medicine Human Genome CC Sequencing Center CC Center code: BCM-HGSC CC Web site: http://www.hgsc.bcm.tmc.edu/cdna/ CC Contact: amg@bcm.tmc.edu CC Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H., CC Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati, CC A.N., Gibbs, R.A. CC Clone distribution: MGC clone distribution information can be found CC through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov CC Series: IRAK Plate: 31 Row: i Column: 22 CC This clone was selected for full length sequencing because it CC passed the following selection criteria: matched mRNA gi: 31981619. CC Differences found between this sequence and the mouse C57BL/6J CC genome (build 36) are described in misc_difference features below. XX FH Key Location/Qualifiers FH FT source 1..2988 FT /organism="Mus musculus" FT /lab_host="DH10B" FT /strain="FVB/N" FT /mol_type="mRNA" FT /clone_lib="NCI_CGAP_Mam1" FT /clone="MGC:25377 IMAGE:4483519" FT /tissue_type="Mammary tumor. Metallothionien-TGF alpha FT model. 10 month old virgin mouse. Taken by biopsy." FT /note="Vector: pCMV-SPORT6" FT /db_xref="taxon:10090" FT gene 1..2988 FT /gene="Mgat1" FT CDS 641..1984 FT /codon_start=1 FT /gene="Mgat1" FT /product="mannoside acetylglucosaminyltransferase 1" FT /db_xref="GOA:Q8CIC9" FT /db_xref="HSSP:1FOA" FT /db_xref="InterPro:IPR004139" FT /db_xref="MGI:96973" FT /db_xref="UniProtKB/TrEMBL:Q8CIC9" FT /protein_id="AAH31752.1" FT /translation="MLKKQTAGLVLWGAIIFVGWNALLLLFFWTRPAPGRLPSDSALGD FT DPASLTREVIHLAEDAEAELERQRGLLQQIKEHYALWRQRWRVPTVAPPAWPRVPVTPS FT PVQIPILVIACDRSTVRRCLDKLLHYRPSAERFPIIVSQDCGHEETAQVIASYGTAVTH FT IRQPDLSNIAVQPDHRKFQGYYKIARHYRWALGQIFNKFKFPAAVVVEDDLEVAPDFFE FT YFQATYPLLRTDPSLWCVSAWNDNGKEQMVDSSKPELLYRTDFFPGLGWLLLADLWAEL FT EPKWPKAFWDDWMRRPEQRKGRACIRPEISRTMTFGRKGVSHGQFFDQHLKFIKLNQQF FT VPFTQLDLSYLQQEAYDRDFLAQVYGAPQLQVENLRTNDQKELGEVRVQYTSRDSFKAF FT AKALGVMDDLKSGVPRAGYRGIVTFQFRGRRVHLAPPQTWTGYDPSWN" FT misc_difference 1762..1763 FT /gene="Mgat1" FT /note="in the mouse genome : AG." FT misc_difference 2607 FT /gene="Mgat1" FT /note="'G' in cDNA is 'T' in the mouse genome." FT misc_difference 2921 FT /gene="Mgat1" FT /note="1 base in cDNA is not found in the mouse genome." FT misc_difference 2978..2988 FT /gene="Mgat1" FT /note="polyA tail: 11 bases do not align to the mouse FT genome." XX SQ Sequence 2988 BP; 602 A; 814 C; 881 G; 691 T; 0 other; ggtggggcct gcgggcggca gggacctggc cccggcctgg atctggagcc gggagggcgg 60 aggctcccag cccggaccga tgggcggggc ccaggttccc acagagatca gcctcccggg 120 ccatgttggt gttaaagcct gtttcttctc ctgagccccg gcagaacaca agtggaccag 180 tggatgactt ctcttgtctg agcaagagat ggcctaatcc ctgcaatctc tctaccactg 240 gcctctgagc tgtaaaagcc agctggatga agggatgtaa cacgcgcctg ggagggacac 300 tgaagtcagg agtggggggg gaggggcccg gacacacccc ttcccccccc agactcctcc 360 ccatcagggt gcacgtggcc atggactttg gacctggata agtggagaga agctgtactt 420 tgggggatct gattctcagt tttggaaatg aagtattagg ttgggggagg gggcggggtt 480 gatgatcttc agggaagcga cagagagaag aaaagaaagg tacatggctt cttcctactc 540 tcagcctaga agaataaacc cttagtgtgg ggacctgagc caggcaagcc aaaggcagcc 600 ttgagccctc cctcccctgc cctcccctgc gggggccagg atgctgaaga agcagactgc 660 agggcttgtg ctttggggtg ctatcatctt tgtgggctgg aatgccctgc tgcttctctt 720 cttctggaca cgcccagcac ctggcaggct gccctcagac agcgcccttg gtgatgaccc 780 tgccagcctc acccgtgagg tcatccacct ggccgaggac gctgaggcgg agttggagcg 840 gcagagggga ctgttgcagc aaatcaagga gcattatgct ttgtggaggc agaggtggag 900 agttcccact gtggcccctc cagcctggcc ccgtgtgcct gtgaccccct caccagtgca 960 gatccccatc ctggtcattg cctgtgaccg cagcactgtc cggcgctgct tggataagtt 1020 gttgcactat cggccctcag ctgagcgttt ccccattatt gtcagtcagg actgtgggca 1080 tgaagagaca gcacaggtca ttgcttccta tggcactgca gtcacacaca tccggcagcc 1140 ggacctgagt aacattgccg tgcagccaga ccaccgcaag ttccagggtt actacaagat 1200 tgccaggcac taccgctggg cactaggcca gatcttcaac aagttcaagt tcccggccgc 1260 tgtggtagtg gaggatgatc tggaagtggc accagacttc tttgagtact tccaggccac 1320 ctacccactg ctgagaacag acccctccct ttggtgtgtg tctgcttgga atgacaatgg 1380 caaggagcag atggtagact caagcaaacc tgagctgctc tatcgaacag acttttttcc 1440 tggccttgga tggctgctgt tggctgatct gtgggcagag ctggagccca agtggcccaa 1500 ggccttttgg gacgactgga tgcgccgacc tgagcagcgg aaaggacggg cttgtattcg 1560 tccagaaatt tcaagaacta tgacctttgg tcgcaagggt gtgagccacg ggcagttctt 1620 tgaccagcat cttaagttca tcaagctgaa ccagcagttc gtccccttca cccagttgga 1680 cctgtcgtac ctgcagcagg aggcctatga ccgggacttc ctcgcccagg tctatggtgc 1740 cccccagcta caggtggaga acttaaggac caatgatcag aaggagctgg gggaggtgcg 1800 ggtacagtac actagcagag acagcttcaa ggcctttgct aaggccctgg gtgtcatgga 1860 tgacctcaag tctggtgtcc ccagggctgg ctaccgtggc attgtcactt tccagtttcg 1920 gggccggcgt gtgcacctgg cacccccaca gacatggact ggctatgatc ctagctggaa 1980 ttagcagcac ctgcctttcc ctcctgggtc tccttgccgc gtgctgagcc gaggcaggcc 2040 tgcagggcct gggctacacc atcctgccag tgtctccctc tcagtgtctc cctcttggat 2100 ctgtagatct tccctttgtg tcctggccag cccccaaatg gcattctagt gcacaaatca 2160 taggatgaga gttatactcc tgttgtcaag ggagtattgt gtggtatgtt cggggcatat 2220 tgaacaagaa accactgtgt ggtatgggga gggttgggcc tgttggggcc aggtttgtaa 2280 tgtctccaag ggcatctgca gagagcttgg cagctccagc tctcctcagc aggcctctcc 2340 accctgatct ggcccctgtt agcccatgag ccctctttat gtacccatcc tccctttctg 2400 aaccttccct cagggtgggg ctggattatg gaggaaggaa tggatctttg gtcagatgag 2460 actaacagag ggactcactg tcagagttag cgtggattgc tgctgccggg gcactacctc 2520 ctggcttctc tccctgctga cgtggctctt gtcctggagc agagcagtgc gttgttggga 2580 gatggaaagt ctatgcgtgg gtacccggtc ttcttgcctg tggcccccct cctttctgaa 2640 agttagttct ctccgcattc tttaggagga ttcaggggcg gcctagtgga agacagtggg 2700 ctccatgcct ctttcccctg gggtttgctt caggggctcc tccccgaggg cttgctcctg 2760 gtggcagcag ctgagatcag gacatctgtg gccctgagcc ggctctggtc ctcggccttt 2820 cccagccttc ccacatcttc ccaggccaag atgcaggggg caggctgaag agaaatttgt 2880 gtgtccttgt ttgttgcctg atcttagttt catggaagaa gaatggaatc tacagaatta 2940 ttttcaaaaa taaagtaaag gctgaattgc tgaaaaaaaa aaaaaaaa 2988 // |
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