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EBI Dbfetch

ID   BC027718; SV 2; linear; mRNA; HTC; HUM; 1450 BP.
XX
AC   BC027718;
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DT   03-MAY-2002 (Rel. 71, Created)
DT   15-OCT-2008 (Rel. 97, Last updated, Version 11)
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DE   Homo sapiens cDNA clone IMAGE:3842599, containing frame-shift errors.
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KW   HTC.
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OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
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RN   [1]
RP   1-1450
RX   DOI; 10.1073/pnas.242603899
RX   PUBMED; 12477932.
RG   Mammalian Gene Collection Program Team
RA   Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D.,
RA   Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F.,
RA   Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H.,
RA   Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K.,
RA   Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F.,
RA   Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S.,
RA   Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J.,
RA   Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J.,
RA   Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M.,
RA   Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X.,
RA   Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA   Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C.,
RA   Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I.,
RA   Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.;
RT   "Generation and initial analysis of more than 15,000 full-length human and
RT   mouse cDNA sequences";
RL   Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002).
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RN   [2]
RC   NIH-MGC Project URL: http://mgc.nci.nih.gov
RP   1-1450
RG   NIH MGC Project
RA   ;
RT   ;
RL   Submitted (08-APR-2002) to the EMBL/GenBank/DDBJ databases.
RL   National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda,
RL   MD 20892-2590, USA
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DR   Ensembl-Gn; ENSG00000152348; Homo_sapiens.
DR   Ensembl-Tr; ENST00000355178; Homo_sapiens.
DR   GOA; Q9H0Y0.
DR   H-InvDB; HIT000050924.
DR   HGNC; 20315; ATG10.
DR   ImaGenes; IMAGp958B08536Q.
DR   ImaGenes; IRALp962F1628Q.
DR   ImaGenes; IRAUp969F02101D.
DR   ImaGenes; IRAUp969F0293D.
DR   InterPro; IPR007135; Autophagy-rel_prot_3.
DR   UniProtKB/Swiss-Prot; Q9H0Y0; ATG10_HUMAN.
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CC   On Aug 19, 2003 this sequence version replaced gi:20379489.
CC   Contact: MGC help desk
CC   Email: cgapbs-r@mail.nih.gov
CC   Tissue Procurement: ATCC
CC   cDNA Library Preparation: Rubin Laboratory
CC   cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
CC   DNA Sequencing by: National Institutes of Health Intramural
CC   Sequencing Center (NISC),
CC   Gaithersburg, Maryland;
CC   Web site: http://www.nisc.nih.gov/
CC   Contact: nisc_mgc@nhgri.nih.gov
CC   Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
CC   Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
CC   Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
CC   Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
CC   Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
CC   McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
CC   Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
CC   Young,A., Zhang,L.-H. and Green,E.D.
CC   Clone distribution: MGC clone distribution information can be found
CC   through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
CC   Series: IRAL Plate: 28 Row: f Column: 16
CC   This clone was selected for full length sequencing because it
CC   passed the following selection criteria: matched mRNA gi: 33589825
CC   This clone has the following problem: frame shifted.
CC   Differences found between this sequence and the human reference
CC   genome (build 36) are described in misc_difference features below
CC   and these differences were also compared to chimpanzee genome
CC   (build 1).
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FH   Key             Location/Qualifiers
FH
FT   source          1..1450
FT                   /organism="Homo sapiens"
FT                   /lab_host="DH10B-R"
FT                   /mol_type="mRNA"
FT                   /clone_lib="NIH_MGC_21"
FT                   /clone="IMAGE:3842599"
FT                   /tissue_type="Placenta, choriocarcinoma"
FT                   /note="Vector: pOTB7"
FT                   /db_xref="taxon:9606"
FT   misc_difference 332^333
FT                   /note="2 bases in the human genome, GA, are not found in
FT                   cDNA. The chimpanzee genome agrees with the human genomic
FT                   sequence and not the cDNA."
FT   misc_difference 891
FT                   /note="'T' in cDNA is 'C' in the human genome."
FT   misc_difference 915
FT                   /note="'A' in cDNA is 'C' in the human genome. The
FT                   chimpanzee genome agrees with the cDNA sequence, suggesting
FT                   that this difference is unlikely to be due to an artifact."
FT   misc_difference 1080
FT                   /note="'A' in cDNA is 'G' in the human genome. The
FT                   chimpanzee genome agrees with the cDNA sequence, suggesting
FT                   that this difference is unlikely to be due to an artifact."
FT   misc_difference 1244
FT                   /note="'T' in cDNA is 'C' in the human genome. The
FT                   chimpanzee genome agrees with the human genomic sequence
FT                   and not the cDNA."
FT   misc_difference 1294
FT                   /note="'G' in cDNA is 'A' in the human genome. The
FT                   chimpanzee genome agrees with the human genomic sequence
FT                   and not the cDNA."
FT   misc_difference 1418..1450
FT                   /note="polyA tail: 33 bases do not align to the human
FT                   genome."
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SQ   Sequence 1450 BP; 397 A; 329 C; 372 G; 352 T; 0 other;
     ctgaaggagg ccgcggacct gactgaagga ggccacggcc acttctggtt ggcctcgggg        60
     cgcgctggct cggctcttcc tccgccctcg aggcccccgc agtcccatca ttcagttccg       120
     tagggtcacc ggcgcggcag tggcctcgca gggcgctggg tccctctccc cagctctcct       180
     ccccctggcc ccgtcgcccc gccctcgccg ggctgggctg cggggtcagg ggccgagcgg       240
     agagggttat catttaacat ggaagaagat gagttcattg gagaaaaaac attccaacgt       300
     tattgtgcag aattcattaa acattcacaa cataggtgat agttgggaat ggagaccatc       360
     aaaggactgt tctgatggct acatgtgcaa aatacacttt caaattaaga atgggtctgt       420
     gatgtcacat ctaggagcat ctacccatgg acagacatgt cttcccatgg aggaggcttt       480
     cgagctaccc ttggatgatt gtgaagtgat tgaaactgca gcagcgtccg aagtgattaa       540
     atatgagtat catgtcttat attcctgtag ctaccaagtg cctgtacttt actttagggc       600
     aagcttttta gatgggagac ctttaactct gaaggacata tgggaaggag ttcatgagtg       660
     ctataagatg cgactgctac agggaccatg ggacactatt acgcaacagg aacatccaat       720
     acttgggcaa cccttttttg tacttcatcc ctgcaagacg aatgaattca tgactcctgt       780
     attaaagaat tctcagaaaa tcaataagaa tgtcaactat atcacatcat ggctgagcat       840
     tgtagggcca gttgttgggc tgaatctacc tctgagttat gccaaagcaa tgtctcagga       900
     tgaacgaaat gtccattaac aagattcttc tattgagttt aggaattgcg gcacgaagaa       960
     tgccaagagt ttacctggcc agccctggct ttaataggac tgataccatg gaatatttca      1020
     tctcaccaag atgtgacatg gattattttt cccttggaca caaatgtcta cagcaactga      1080
     tgtttgatag gctgaatgtt tagaagaaac acttcaaagg gatacatcat ggccaggcat      1140
     ggtggctcac acctgtaatc caagcacttt gggaggccaa ggtgggagca tcacttgatc      1200
     ctgggagttc gagaccagcc tgggcaacat ggtgaaaccc tgttggtaca aaaaaataca      1260
     aaaatttgcc tgtttatggt ggtgtgttcc tgtggtccca gctccccagg aggctgaggt      1320
     gggaggttgg ctttaaccca ggaggcagag gttgcagtga gctgagactg tgccactgca      1380
     gtccagcctg ggtgacagag ccagacactg tctcgggaaa aaaaaaaaaa aaaaaaaaaa      1440
     aaaaaaaaaa                                                             1450
//


  
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