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EBI DbfetchID BC019464; SV 1; linear; mRNA; STD; MUS; 1427 BP. XX AC BC019464; XX DT 04-JAN-2002 (Rel. 70, Created) DT 24-SEP-2008 (Rel. 97, Last updated, Version 13) XX DE Mus musculus ubiquitin-conjugating enzyme E2D 1, UBC4/5 homolog (yeast), DE mRNA (cDNA clone MGC:28550 IMAGE:4205941), complete cds. XX KW MGC. XX OS Mus musculus (house mouse) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; OC Muridae; Murinae; Mus. XX RN [1] RP 1-1427 RX DOI; 10.1073/pnas.242603899 RX PUBMED; 12477932. RG Mammalian Gene Collection Program Team RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., RA Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., RA Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., RA Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K., RA Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F., RA Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S., RA Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J., RA Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J., RA Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M., RA Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., RA Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., RA Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C., RA Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I., RA Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human and RT mouse cDNA sequences"; RL Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002). XX RN [2] RC NIH-MGC Project URL: http://mgc.nci.nih.gov RP 1-1427 RG NIH MGC Project RA ; RT ; RL Submitted (19-DEC-2001) to the EMBL/GenBank/DDBJ databases. RL National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda, RL MD 20892-2590, USA XX DR ASTD; TRAN00000160832. DR Ensembl-Gn; ENSMUSG00000019927; Mus_musculus. DR Ensembl-Tr; ENSMUST00000020085; Mus_musculus. DR ImaGenes; IMAGp998E149766Q. DR ImaGenes; IRAKp961G1237Q. DR ImaGenes; IRAVp968D0234D. DR ImaGenes; IRAVp968G049Q. XX CC Contact: MGC help desk CC Email: cgapbs-r@mail.nih.gov CC Tissue Procurement: Jeffrey E. Green, M.D. CC cDNA Library Preparation: Life Technologies, Inc. CC cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) CC DNA Sequencing by: Baylor College of Medicine Human Genome CC Sequencing Center CC Center code: BCM-HGSC CC Web site: http://www.hgsc.bcm.tmc.edu/cdna/ CC Contact: amg@bcm.tmc.edu CC Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H., CC Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati, CC A.N., Gibbs, R.A. CC Clone distribution: MGC clone distribution information can be found CC through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov CC Series: IRAK Plate: 37 Row: g Column: 12 CC This clone was selected for full length sequencing because it CC passed the following selection criteria: Similarity but not CC identity to protein. CC Differences found between this sequence and the mouse C57BL/6J CC genome (build 36) are described in misc_difference features below. XX FH Key Location/Qualifiers FH FT source 1..1427 FT /organism="Mus musculus" FT /lab_host="DH10B" FT /strain="FVB/N" FT /mol_type="mRNA" FT /clone_lib="NCI_CGAP_Co24" FT /clone="MGC:28550 IMAGE:4205941" FT /tissue_type="Colon, normal. 5 month old male mouse." FT /note="Vector: pCMV-SPORT6" FT /db_xref="taxon:10090" FT gene 1..1427 FT /gene="Ube2d1" FT /note="synonyms: UBCH5, MGC28550" FT CDS 110..553 FT /codon_start=1 FT /gene="Ube2d1" FT /product="ubiquitin-conjugating enzyme E2D 1, UBC4/5 FT homolog (yeast)" FT /db_xref="GOA:P61080" FT /db_xref="InterPro:IPR000608" FT /db_xref="MGI:2384911" FT /db_xref="UniProtKB/Swiss-Prot:P61080" FT /protein_id="AAH19464.1" FT /translation="MALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAY FT QGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLL FT SICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYAM" FT misc_difference 1284^1285 FT /gene="Ube2d1" FT /note="1 base in the mouse genome, T, is not found in FT cDNA." FT misc_difference 1319 FT /gene="Ube2d1" FT /note="1 base in cDNA is not found in the mouse genome." FT misc_difference 1403..1427 FT /gene="Ube2d1" FT /note="polyA tail: 25 bases do not align to the mouse FT genome." XX SQ Sequence 1427 BP; 376 A; 366 C; 275 G; 410 T; 0 other; ggcgccgccg cccgcacact cgcgcaccgc gcccgggcgc acacggagca gccctgcgag 60 gcggcagccc gggacacgcg gacactccgc agcccccgcc gcctgcccca tggcgctgaa 120 gcggatccag aaagaattaa gtgatttaca gcgtgatcca cctgcccact gctcagcggg 180 acccgtggga gatgacttgt tccactggca agcaaccatc atggggcccc ctgacagcgc 240 ctatcaaggt ggagtcttct tcctcactgt ccactttccg acagactatc cctttaaacc 300 accaaagatt gctttcacaa caaaaatcta ccacccaaat ataaacagca acgggagtat 360 ttgtcttgac atcctgaggt cccagtggtc gcccgctttg actgtatcga aagtcttgct 420 gtccatatgc tcgctgctct gtgatcctaa tccagacgat cccttagtac cagatattgc 480 acagatctat aaatcagaca aagaaaaata caacaggcac gcaagagaat ggactcagaa 540 atatgcaatg taaagtttca aacactttca cttacgccag agtactgtaa aatctatgtc 600 ttttccagcg ttagcattaa gccgggcgcc gtttgccgtg tccttcgttc cacgagccct 660 ttggttttta ctcattttaa gtgtgtttct gaagcaagac aaaacaaact tccaagaata 720 ccctggaaac tctgctgaga gcatttagta ttctgtgctc attgaagagg acctttcttt 780 cggtttccga gacacttgga ccccctgcat ctccagcaag atctgtgacg tgactgagaa 840 agcaaccaga cgcttttctt tctctgaaac gagacgtttt tcacccgaga aacattgtat 900 gtgccgtcca agatgtcgtc acttttatac atctggattc agatctcatt cttcgttagc 960 tcactttata atttgtattt ttttactgta tagactaaat atattctatg tcaactcctg 1020 actcctcctc cccaccccca tcccccgtga acagtattga agctcccaac agctgatgat 1080 gaattaactg cccccgcccc ttgtcttagc atattctgtg ggttgaatgc gtatttctta 1140 aaccggaact gatctgcaca ctgtatttct cagtatactg attagaatgg agaaacactt 1200 gtttgggttt tattgtactt gacttaactt tattgcaatg tgaattaatt gcactgttag 1260 gtaggaaggt gtgtgacttt ttttgttgtt gctcttcacc tttgaccttt tttttttttc 1320 tgtataggca atattatatt gacacctttt acagatctga ctgtagcctt ttccatataa 1380 ataaaatgct ttttctacta ttaaaaaaaa aaaaaaaaaa aaaaaaa 1427 // |
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