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EBI DbfetchID BC008638; SV 1; linear; mRNA; STD; MUS; 2152 BP. XX AC BC008638; XX DT 08-JUN-2001 (Rel. 68, Created) DT 24-SEP-2008 (Rel. 97, Last updated, Version 11) XX DE Mus musculus aspartyl-tRNA synthetase, mRNA (cDNA clone MGC:6719 DE IMAGE:3586278), complete cds. XX KW MGC. XX OS Mus musculus (house mouse) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; OC Muridae; Murinae; Mus. XX RN [1] RP 1-2152 RX DOI; 10.1073/pnas.242603899 RX PUBMED; 12477932. RG Mammalian Gene Collection Program Team RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., RA Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., RA Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., RA Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K., RA Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F., RA Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S., RA Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J., RA Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J., RA Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M., RA Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., RA Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., RA Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C., RA Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I., RA Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human and RT mouse cDNA sequences"; RL Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002). XX RN [2] RC NIH-MGC Project URL: http://mgc.nci.nih.gov RP 1-2152 RG NIH MGC Project RA ; RT ; RL Submitted (25-MAY-2001) to the EMBL/GenBank/DDBJ databases. RL National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda, RL MD 20892-2590, USA XX DR ASTD; TRAN00000121062. DR Ensembl-Gn; ENSMUSG00000026356; Mus_musculus. DR Ensembl-Tr; ENSMUST00000064309; Mus_musculus. DR ImaGenes; IMAGp998J078747Q. DR ImaGenes; IRAKp961N2310Q. DR ImaGenes; IRAVp968D0114D. DR ImaGenes; IRAVp968G024Q. XX CC Contact: MGC help desk CC Email: cgapbs-r@mail.nih.gov CC Tissue Procurement: Gilbert Smith, Ph.D. CC cDNA Library Preparation: Life Technologies, Inc. CC cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) CC DNA Sequencing by: Baylor College of Medicine Human Genome CC Sequencing Center CC Center code: BCM-HGSC CC Web site: http://www.hgsc.bcm.tmc.edu/cdna/ CC Contact: amg@bcm.tmc.edu CC Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H., CC Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati, CC A.N., Gibbs, R.A. CC Clone distribution: MGC clone distribution information can be found CC through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov CC Series: IRAK Plate: 10 Row: n Column: 23 CC This clone was selected for full length sequencing because it CC passed the following selection criteria: matched mRNA gi: 21703997. CC Differences found between this sequence and the mouse C57BL/6J CC genome (build 36) are described in misc_difference features below. XX FH Key Location/Qualifiers FH FT source 1..2152 FT /organism="Mus musculus" FT /lab_host="DH10B" FT /strain="FVB/N" FT /mol_type="mRNA" FT /clone_lib="NCI_CGAP_Mam1" FT /clone="MGC:6719 IMAGE:3586278" FT /tissue_type="Mammary tumor. Metallothionien-TGF alpha FT model. 10 month old virgin mouse. Taken by biopsy." FT /note="Vector: pCMV-SPORT6" FT /db_xref="taxon:10090" FT gene 1..2152 FT /gene="Dars" FT CDS 98..1603 FT /codon_start=1 FT /gene="Dars" FT /product="aspartyl-tRNA synthetase" FT /db_xref="GOA:Q922B2" FT /db_xref="HSSP:1ASZ" FT /db_xref="InterPro:IPR004365" FT /db_xref="MGI:2442544" FT /db_xref="UniProtKB/Swiss-Prot:Q922B2" FT /protein_id="AAH08638.1" FT /translation="MPSANASRKGQEKPREIVDAAEDYAKERYGISSMIQSQEKPDRVL FT VRVKDLTVQKADDVVWVRARVHTSRAKGKQCFLVLRQQQFNVQALVAVGDHASKQMVKF FT AANINKESIIDVEGVVRKVNQKIGSCTQQDVELHVQKIYVISLAEPRLPLQLDDAIRPE FT VEGEEDGRATVNQDTRLDNRVIDLRTSTSQAIFRLQSGICHLFRETLINKGFVEIQTPK FT IISAASEGGANVFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFCIGPVFRAEDSNTHR FT HLTEFVGLDIEMAFNYHYHEVVEEIADTLVQIFKGLQERFQTEIQTVSKQFPCEPFKFL FT EPTLRLEYCEALAMLREAGVEMDDEEDLSTPNEKLLGRLVKEKYDTDFYVLDKYPLAVR FT PFYTMPDPRNPKQSNSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDS FT FRFGAPPHAGGGIGLERVTMLFLGLHNVRQTSMFPRDPKRLTP" FT misc_difference 107 FT /gene="Dars" FT /note="'G' in cDNA is 'A' in the mouse genome; amino acid FT difference: 'A' in cDNA, 'T' in the mouse genome." FT misc_difference 217 FT /gene="Dars" FT /note="'A' in cDNA is 'G' in the mouse genome; no amino FT acid change." FT misc_difference 412 FT /gene="Dars" FT /note="'A' in cDNA is 'T' in the mouse genome; no amino FT acid change." FT misc_difference 616 FT /gene="Dars" FT /note="'C' in cDNA is 'G' in the mouse genome; no amino FT acid change." FT misc_difference 766 FT /gene="Dars" FT /note="'T' in cDNA is 'A' in the mouse genome; no amino FT acid change." FT misc_difference 1138 FT /gene="Dars" FT /note="'A' in cDNA is 'G' in the mouse genome; no amino FT acid change." FT misc_difference 1771 FT /gene="Dars" FT /note="'T' in cDNA is 'A' in the mouse genome." FT misc_difference 1827 FT /gene="Dars" FT /note="'T' in cDNA is 'C' in the mouse genome." FT misc_difference 1877 FT /gene="Dars" FT /note="'C' in cDNA is 'T' in the mouse genome." FT misc_difference 1975^1976 FT /gene="Dars" FT /note="1 base in the mouse genome, T, is not found in FT cDNA." FT misc_difference 2029 FT /gene="Dars" FT /note="1 base in cDNA is not found in the mouse genome." FT misc_difference 2083..2084 FT /gene="Dars" FT /note="in the mouse genome : CA." FT misc_difference 2131..2152 FT /gene="Dars" FT /note="polyA tail: 22 bases do not align to the mouse FT genome." XX SQ Sequence 2152 BP; 681 A; 391 C; 477 G; 603 T; 0 other; gggagggcga gactgtgcgg gagggagccg cttcttagcc tgtcctacgg aagcctgcga 60 gggagggtgg tatccactcc ccaaatccag tgtcctgatg cccagcgcca acgccagccg 120 taagggtcag gagaagccgc gggagatcgt ggacgcggcg gaagattatg ctaaagagag 180 atatgggata tcttctatga tacaatcaca agaaaaacca gatagagttt tggttcgagt 240 taaggacctg acagttcaaa aagctgatga tgttgtttgg gtccgtgcaa gagttcatac 300 aagcagagca aaagggaagc agtgcttctt agtcctgagg cagcagcagt ttaatgtcca 360 ggctcttgtg gcggtgggag accatgcaag caagcagatg gttaaatttg cagccaacat 420 caacaaagaa agtattattg acgtagaagg tgttgtgagg aaagtcaatc agaaaattgg 480 aagctgtacc cagcaggatg ttgagttgca tgtccagaag atttatgtga tcagcttggc 540 tgaacctcgc ctgcccttgc agctggatga tgccattcgg cctgaagtgg aaggagaaga 600 ggacggaaga gctaccgtta accaggatac aagattagac aacagagtca ttgaccttag 660 gacatcaact agtcaggcca tcttccgtct ccagtccggc atctgccatc tcttccgaga 720 aactttaatt aacaaaggtt ttgtggaaat ccaaactcct aaaattattt cagctgccag 780 tgaaggagga gccaatgttt ttactgtgtc ctattttaaa aataatgcct accttgctca 840 gtctccacaa ttgtataagc agatgtgcat ttgtgctgat tttgagaagg ttttctgcat 900 tggaccagtt ttcagagctg aagactccaa tacccataga catctgactg aatttgttgg 960 tttggatatt gaaatggcct ttaattatca ttaccatgaa gttgtagaag agattgctga 1020 cactttggtg caaatattca aaggacttca agaaaggttt cagactgaaa ttcaaactgt 1080 gagtaaacag ttcccatgtg agccgttcaa atttttggag ccaactttaa gactggaata 1140 ttgcgaggct ttggcaatgc ttagagaagc tggagttgaa atggacgatg aggaagatct 1200 aagtacacca aatgaaaaac tgttgggtcg tttggtgaag gaaaagtatg atacagactt 1260 ttatgttctt gataaatatc cattggctgt aagacctttc tataccatgc ctgacccaag 1320 aaatccgaaa cagtccaact cttacgatat gttcatgaga ggagaagaaa tattgtctgg 1380 agcacaaaga atccatgatc ctcagttgct tacagagaga gctttacacc atggaattga 1440 tttggagaaa atcaaagctt acattgattc cttccgcttt ggagctcctc ctcatgctgg 1500 tggtggcatt gggctggaaa gggtgacgat gctgttttta ggattgcata acgtacgcca 1560 gacctcaatg ttccctcgtg accccaaacg actcacgcct taaagccaca ctttttattc 1620 tctccaggaa cctgctattg attaggttat acctgaggta cttaaaatat gcagtagaat 1680 taatgtctta ctaaagtgcc acaattattt tttaagtagc tcagtacaaa ttaatttaag 1740 taaaaaacgt tttagaattt caaatatcct taaataggaa atacttgaac gttttaatga 1800 gaaatttcta tggttgaaat tcatagtctt tcactatagg aaatgttaag aaaaatttaa 1860 acagtttaag attatacaat ttttttttct tttctatatt gaggtggcca ccatactaat 1920 attctaaatt ttcaatattt gaacttttaa ctcttgacat tgaaaaaaaa ggattggaga 1980 aattaccaag attttaacat ctataaaatt ggtatgtctt aaaagttact ttcaaaaatc 2040 ctgtgaagca tctgaaactg gctagaacca gtaacactga gatgttgtta ccagtcctga 2100 ggctgagatt caataaaaac ttacagctga aaaaaaaaaa aaaaaaaaaa aa 2152 // |
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