EBI Dbfetch

ID   BC008116; SV 1; linear; mRNA; STD; MUS; 1500 BP.
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AC   BC008116;
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DT   31-MAY-2001 (Rel. 67, Created)
DT   24-SEP-2008 (Rel. 97, Last updated, Version 18)
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DE   Mus musculus GDP-mannose pyrophosphorylase A, mRNA (cDNA clone MGC:7719
DE   IMAGE:3498021), complete cds.
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KW   MGC.
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OS   Mus musculus (house mouse)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea;
OC   Muridae; Murinae; Mus.
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RN   [1]
RP   1-1500
RX   DOI; 10.1073/pnas.242603899
RX   PUBMED; 12477932.
RG   Mammalian Gene Collection Program Team
RA   Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D.,
RA   Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F.,
RA   Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H.,
RA   Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K.,
RA   Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F.,
RA   Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S.,
RA   Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J.,
RA   Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J.,
RA   Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M.,
RA   Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X.,
RA   Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA   Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C.,
RA   Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I.,
RA   Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.;
RT   "Generation and initial analysis of more than 15,000 full-length human and
RT   mouse cDNA sequences";
RL   Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002).
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RN   [2]
RC   NIH-MGC Project URL: http://mgc.nci.nih.gov
RP   1-1500
RG   NIH MGC Project
RA   ;
RT   ;
RL   Submitted (22-MAY-2001) to the EMBL/GenBank/DDBJ databases.
RL   National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda,
RL   MD 20892-2590, USA
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DR   ASTD; TRAN00000174919.
DR   ASTD; TRAN00000174920.
DR   ASTD; TRAN00000174921.
DR   Ensembl-Gn; ENSMUSG00000033021; Mus_musculus.
DR   Ensembl-Tr; ENSMUST00000113584; Mus_musculus.
DR   ImaGenes; IMAGp998L228552Q.
DR   ImaGenes; IRAKp961C099Q.
DR   ImaGenes; IRAVp968D0413D.
DR   ImaGenes; IRAVp968G074Q.
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CC   Contact: MGC help desk
CC   Email: cgapbs-r@mail.nih.gov
CC   Tissue Procurement: Jeffrey Green M.D.
CC   cDNA Library Preparation: Life Technologies, Inc.
CC   cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
CC   DNA Sequencing by: Baylor College of Medicine Human Genome
CC   Sequencing Center
CC   Center code: BCM-HGSC
CC   Web site: http://www.hgsc.bcm.tmc.edu/cdna/
CC   Contact: amg@bcm.tmc.edu
CC   Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
CC   Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
CC   A.N., Gibbs, R.A.
CC   Clone distribution: MGC clone distribution information can be found
CC   through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
CC   Series: IRAK Plate: 9 Row: c Column: 9
CC   This clone was selected for full length sequencing because it
CC   passed the following selection criteria: matched mRNA gi: 19526883.
CC   Differences found between this sequence and the mouse C57BL/6J
CC   genome (build 36) are described in misc_difference features below.
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FH   Key             Location/Qualifiers
FH
FT   source          1..1500
FT                   /organism="Mus musculus"
FT                   /lab_host="DH10B"
FT                   /strain="FVB/N"
FT                   /mol_type="mRNA"
FT                   /clone_lib="NCI_CGAP_Mam6"
FT                   /clone="MGC:7719 IMAGE:3498021"
FT                   /tissue_type="Mammary tumor. C3(1)-Tag model. Infiltrating
FT                   ductal carcinoma. 5 month old virgin mouse."
FT                   /note="Vector: pCMV-SPORT6"
FT                   /db_xref="taxon:10090"
FT   gene            1..1500
FT                   /gene="Gmppa"
FT   misc_difference 39
FT                   /gene="Gmppa"
FT                   /note="'C' in cDNA is 'T' in the mouse genome."
FT   CDS             50..1312
FT                   /codon_start=1
FT                   /gene="Gmppa"
FT                   /product="GDP-mannose pyrophosphorylase A"
FT                   /db_xref="GOA:Q922H4"
FT                   /db_xref="InterPro:IPR005835"
FT                   /db_xref="MGI:1916330"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q922H4"
FT                   /protein_id="AAH08116.1"
FT                   /translation="MLKAVILIGGPQKGTRFRPLSFEVPKPLFPVAGVPMIQHHIEACA
FT                   QVPGMQEILLIGFYQPDEALTQFLEAAQQEFNLPVRYLQEFAPLGTGGGLYHFRDQILA
FT                   GAPEAFFVLNADVCSDFPLSAMLEAHRRQRHPFLLLGTTANRTQSLNYGCIVENPQTHE
FT                   VLHYVEKPSTFISDIINCGIYLFSPEALKPLRDVFQRNQQDGQLEESPGSWPGAGTIRL
FT                   EQDVFSALAGQGQIYVHLTDGIWSQIKSAGSALYASRLYLGRYQITHPERLARHTPGGP
FT                   RIRGNVYIHPTAKVAPSAVLGPNVSIGKGVTIGEGVRLRESIVLHGATLQEHTCVLHSI
FT                   VGWGSTVGRWARVEGTPNDPNPNDPRARMDSESLFKDGKLLPAITILGCRVRIPAEVLI
FT                   LNSIVLPHKELSRSFTNQIIL"
FT   misc_difference 1255
FT                   /gene="Gmppa"
FT                   /note="'G' in cDNA is 'A' in the mouse genome; no amino
FT                   acid change."
FT   misc_difference 1485..1500
FT                   /gene="Gmppa"
FT                   /note="polyA tail: 16 bases do not align to the mouse
FT                   genome."
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SQ   Sequence 1500 BP; 326 A; 457 C; 393 G; 324 T; 0 other;
     ggcagagctg gcgtaaaggg agctagtggt ttaggtaaca gcgctcatca tgctcaaagc        60
     tgtgattctc attggaggcc cccagaaggg gactcgcttc aggcctttgt cttttgaggt       120
     gcccaaacct ctgtttcctg tggcaggcgt tcccatgatc cagcaccata tagaagcctg       180
     tgcccaggtc ccagggatgc aggagattct tctcattggc ttctaccagc ctgatgaggc       240
     cctcacccag ttcctggaag ctgcccagca ggagtttaac cttccagtca ggtacctgca       300
     ggagtttgcc cccctcggca cagggggtgg cctctaccat tttcgggacc agatcctggc       360
     tggggcacct gaggccttct tcgtgctcaa tgctgacgtc tgctctgact tccccttgag       420
     cgccatgttg gaggctcaca ggcgccagcg ccaccctttc ttactccttg gcaccacggc       480
     taacaggaca caatccctca actacggctg catcgttgag aatccacaga ctcatgaggt       540
     tctgcactat gtggagaaac ccagcacctt tatcagtgac atcatcaact gtggcatcta       600
     ccttttctcc ccagaagccc tgaagcctct ccgggatgtt ttccagcgta accaacagga       660
     tgggcaactg gaggagtccc caggctcgtg gcctggcgca gggaccatcc gactagaaca       720
     ggacgtgttc tcagccctgg ctgggcaggg ccaaatctac gtgcacctca ccgatggtat       780
     ctggagccag atcaagtctg caggctcagc cctttatgcc tcccgcctct atctggggcg       840
     ataccagatc actcacccag aacggctagc cagacacact ccagggggcc cacgcatcag       900
     aggaaatgtt tacatccatc caaccgctaa agtggcccca tcagctgtgc tgggccccaa       960
     tgtctccatt gggaaggggg tgaccatagg cgagggtgtg cgtctgcgag agagcattgt      1020
     cctccatgga gccactttgc aggaacacac ctgcgtcctt cacagcattg tgggctgggg      1080
     gagcactgtg gggcgctggg cccgtgtaga ggggactccc aatgacccca atccgaatga      1140
     cccccgagct cgcatggaca gtgagagcct cttcaaagat ggaaagctgc ttcctgccat      1200
     cactatcctg ggctgccgtg ttcggatccc tgccgaggtg ctcatcttga actcgattgt      1260
     tctaccacat aaggagctaa gtcgaagctt caccaaccaa atcatcctgt gaggatgctg      1320
     ccaaaaggcc cccaggactc ccgcccacac tcctttgggc tgctgcctgc ctggctagct      1380
     ctgtccagat aggacctgag gaagccacct tgtagggagc cttgtgtgtg gccagggaca      1440
     cctggcttcc ctccctactg ccaaataaac ccctatgaac cttgaaaaaa aaaaaaaaaa      1500
//