EBI Dbfetch

ID   BC006882; SV 1; linear; mRNA; STD; MUS; 1590 BP.
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AC   BC006882;
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DT   02-MAY-2001 (Rel. 67, Created)
DT   24-SEP-2008 (Rel. 97, Last updated, Version 16)
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DE   Mus musculus pyrroline-5-carboxylate reductase family, member 2, mRNA (cDNA
DE   clone MGC:6271 IMAGE:2647612), complete cds.
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KW   MGC.
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OS   Mus musculus (house mouse)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea;
OC   Muridae; Murinae; Mus.
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RN   [1]
RP   1-1590
RX   DOI; 10.1073/pnas.242603899
RX   PUBMED; 12477932.
RG   Mammalian Gene Collection Program Team
RA   Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D.,
RA   Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F.,
RA   Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H.,
RA   Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K.,
RA   Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F.,
RA   Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S.,
RA   Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J.,
RA   Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J.,
RA   Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M.,
RA   Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X.,
RA   Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA   Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C.,
RA   Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I.,
RA   Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., Marra M.A.;
RT   "Generation and initial analysis of more than 15,000 full-length human and
RT   mouse cDNA sequences";
RL   Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002).
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RN   [2]
RC   NIH-MGC Project URL: http://mgc.nci.nih.gov
RP   1-1590
RG   NIH MGC Project
RA   ;
RT   ;
RL   Submitted (27-APR-2001) to the EMBL/GenBank/DDBJ databases.
RL   National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda,
RL   MD 20892-2590, USA
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DR   ASTD; TRAN00000173754.
DR   Ensembl-Gn; ENSMUSG00000026520; Mus_musculus.
DR   Ensembl-Tr; ENSMUST00000027802; Mus_musculus.
DR   ImaGenes; IMAGp998C056607Q.
DR   ImaGenes; IRAKp961A215Q.
DR   ImaGenes; IRAVp968A0610D.
DR   ImaGenes; IRAVp968A123Q.
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CC   Contact: MGC help desk
CC   Email: cgapbs-r@mail.nih.gov
CC   Tissue Procurement: Gilbert Smith, Ph.D.
CC   cDNA Library Preparation: Life Technologies, Inc.
CC   cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
CC   DNA Sequencing by: Baylor College of Medicine Human Genome
CC   Sequencing Center
CC   Center code: BCM-HGSC
CC   Web site: http://www.hgsc.bcm.tmc.edu/cdna/
CC   Contact: amg@bcm.tmc.edu
CC   Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
CC   Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
CC   A.N., Gibbs, R.A.
CC   Clone distribution: MGC clone distribution information can be found
CC   through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
CC   Series: IRAK Plate: 5 Row: a Column: 21
CC   This clone was selected for full length sequencing because it
CC   passed the following selection criteria: matched mRNA gi: 19526877.
CC   Differences found between this sequence and the mouse C57BL/6J
CC   genome (build 36) are described in misc_difference features below.
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FH   Key             Location/Qualifiers
FH
FT   source          1..1590
FT                   /organism="Mus musculus"
FT                   /lab_host="DH10B"
FT                   /strain="FVB/N"
FT                   /mol_type="mRNA"
FT                   /clone_lib="NCI_CGAP_Mam1"
FT                   /clone="MGC:6271 IMAGE:2647612"
FT                   /tissue_type="Mammary tumor. Metallothionien-TGF alpha
FT                   model. 10 month old virgin mouse. Taken by biopsy."
FT                   /note="Vector: pCMV-SPORT6"
FT                   /db_xref="taxon:10090"
FT   gene            1..1590
FT                   /gene="Pycr2"
FT   misc_difference 1..11
FT                   /gene="Pycr2"
FT                   /note="11 bases at the 5' end do not align to the mouse
FT                   genome."
FT   CDS             167..1129
FT                   /codon_start=1
FT                   /gene="Pycr2"
FT                   /product="pyrroline-5-carboxylate reductase family, member
FT                   2"
FT                   /db_xref="GOA:Q922Q4"
FT                   /db_xref="InterPro:IPR000304"
FT                   /db_xref="InterPro:IPR004455"
FT                   /db_xref="InterPro:IPR016040"
FT                   /db_xref="MGI:1277956"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q922Q4"
FT                   /protein_id="AAH06882.1"
FT                   /translation="MSVGFIGAGQLACALARGFTAAGVLSAHKIIASSPDMDLPTVSAL
FT                   RRMGVNLTRSNKDTVRHSDVLFLAVKPHIIPFILDEIGADVQERHIVVSCAAGVTISSV
FT                   EKKLMAFQPAPKVIRCMTNTPVVVREGATVYATGTHALVEDGKLLEQLMSSVGFCTEVE
FT                   EDLIDAITGLSGSGPAYAFMALDALADGGVKMGVPRRLAVRLGAQALLGAAKMLLDSED
FT                   HPGQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTRELQSMADQEKVSPAA
FT                   LKKTLLDRVKLESPTVSTLAPPSSGKLLTRNPAQGSKKE"
FT   misc_difference 1576..1590
FT                   /gene="Pycr2"
FT                   /note="polyA tail: 15 bases do not align to the mouse
FT                   genome."
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SQ   Sequence 1590 BP; 325 A; 436 C; 496 G; 333 T; 0 other;
     cccacgcgtc cgctctggga tacttggggc ctctctagca gttcatctgg tgcatcccgg        60
     tttgaaagct gttccgagca attcgggtcc atctttttcc acgcgcggac actgaccccg       120
     ggatccccag agtccccgga tcggcgctcg ccgggatcct gcggccatga gcgtgggttt       180
     catcggcgca ggccagctgg cctgtgctct ggcgcggggc ttcacggccg cgggcgtcct       240
     gtcggctcac aagataatag ccagctcccc ggatatggac ctgcccacgg tgtctgcgct       300
     caggaggatg ggtgtgaatc tgacccgaag caataaggac actgtgcggc acagcgacgt       360
     cctgttcctg gctgtgaagc cccacatcat ccccttcatc ctggatgaga ttggagccga       420
     cgtgcaggag aggcacatcg tggtctcctg tgcagccggg gtcaccatca gttctgtgga       480
     gaagaagctg atggctttcc agccggcccc caaagtgatc cgctgcatga ccaacacgcc       540
     cgtggtggtc cgagaggggg ccaccgtgta tgccacgggc acccatgctc tggtggaaga       600
     cggcaagctt ttggagcagc tgatgagcag tgtgggcttc tgcacagagg tggaggaaga       660
     cctcattgat gctatcaccg ggctcagcgg cagcgggcct gcctatgcat tcatggccct       720
     ggacgccctg gcagatggtg gggtgaagat gggcgtgccg cggcgcctgg cggtcagact       780
     gggagctcag gcgttgctgg gagcagctaa gatgctgctg gactcagagg accatccagg       840
     ccagctcaag gacaacgtct gctcccctgg gggggccacc atccatgccc tgcacttcct       900
     ggagagtggg ggcttccgct ctctgctcat caatgcggtt gaggcctcct gtatccgaac       960
     cagagaactg cagtccatgg ctgaccaaga aaaggtgtct cctgctgccc ttaagaagac      1020
     ccttctggac agagtgaagc tggagtcacc cacggtgtcc acgctggccc cacccagctc      1080
     agggaaactc cttacaagaa accccgccca gggaagcaag aaggagtgag gaggactctg      1140
     tagggtgtcc ctgtcccctg tagtacaggg aggggcaggc tcaggtctgg gggtgaagct      1200
     gcttacatct gttcacctgg tgccaccgag tccttctcct gtcccgtggc agaaggtgct      1260
     ctgaagaggc ggctgatgcc agaagtcagc atgcctctct gagagaggtg gaggccttag      1320
     agttagcatc cccctcagcg gaagtctgtg gagagcttta gttagatccg aggctgcttc      1380
     agattgatct ccaggtgagg tgagaggaaa cgggaatcct ctgaattaaa aacacgcaaa      1440
     gaagcctgtg tcttggtgtt gcaggtggaa gtgtggggag tcatcctgtg acctcttgct      1500
     taactcttct ggacaggtga cccattagaa ggtgatgttc agggctgctt taataaagat      1560
     cttatttatt tttgaaaaaa aaaaaaaaag                                       1590
//