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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AY693065; SV 1; linear; genomic DNA; STD; FUN; 963 BP.
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AC   AY693065;
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DT   11-AUG-2004 (Rel. 80, Created)
DT   01-MAY-2007 (Rel. 91, Last updated, Version 2)
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DE   Saccharomyces cerevisiae clone FLH113113.01X YMR180C gene, complete cds.
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KW   Yeast ORF Project.
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OS   Saccharomyces cerevisiae (baker's yeast)
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
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RN   [1]
RP   1-963
RX   DOI; 10.1101/gr.6037607
RX   PUBMED; 17322287.
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae";
RL   Genome Res. 17(4):536-543(2007).
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RN   [2]
RP   1-963
RA   Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E.,
RA   Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., Zuo D.,
RA   Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., Gonzaga L.,
RA   Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., LaBaer J.;
RT   "Creation of the YFLEX clone resource: cloning of Saccharomyces cerevisiae
RT   ORFs in the Gateway recombinational cloning system";
RL   Unpublished.
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RN   [3]
RP   1-963
RA   Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E.,
RA   Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., Zuo D.,
RA   Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., Gonzaga L.,
RA   Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., LaBaer J.;
RT   ;
RL   Submitted (20-JUL-2004) to the EMBL/GenBank/DDBJ databases.
RL   Biological Chemistry and Molecular Pharmacology, Harvard Institute of
RL   Proteomics, 320 Charles st., Cambridge, MA 02141, USA
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DR   ImaGenes; RZPDe861F01124D.
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CC   This clone is part of a collection of Saccharomyces cerevisiae
CC   full-length ORF clones generated by the Harvard Institute of
CC   Proteomics. Each CDS has been cloned with its native stop-codon.
CC   The CDS has been directionally cloned using the Gateway  cloning
CC   system into the donor vectors pDONR 201 or pDONR 221. Additional
CC   sequences in the clone:  'TCCAGCTGACCACC' after the attL1 site and
CC   before the 'ATG' (from Research Genetics primers used to amplify
CC   the ORFs, including a Kozak consensus sequence);
CC   'ATCCCCGGGAATTGCCATG' after the stop codon and before the attL2
CC   site (from the Research Genetics primers used to amplify the ORFs).
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FH   Key             Location/Qualifiers
FH
FT   source          1..963
FT                   /organism="Saccharomyces cerevisiae"
FT                   /lab_host="DH5alpha T1 resistant"
FT                   /mol_type="genomic DNA"
FT                   /clone="FLH113113.01X"
FT                   /db_xref="taxon:4932"
FT   mRNA            <1..>963
FT                   /product="YMR180C"
FT   CDS             1..963
FT                   /codon_start=1
FT                   /product="YMR180C"
FT                   /db_xref="GOA:Q03220"
FT                   /db_xref="HSSP:1D8I"
FT                   /db_xref="InterPro:IPR004206"
FT                   /db_xref="SGD:S000004792"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q03220"
FT                   /protein_id="AAT93084.1"
FT                   /translation="MSDQPETPSNSRNSHENVGAKKADANVASKFRSLHISETTKPLTS
FT                   TRALYKTTRNNSRGATEFHKHVCKLAWKYLACIDKSSISHIEIEMKFGVITDKRTHRRM
FT                   TPHNKPFIVQNRNGRLVSNVPEQMFSSFQELLRSKSENPSKCAPRVVKQVQKYTKDSIY
FT                   NCNNASKVGKLTSWRCSEDLRNKELKLTYIKKVRVKDFLIRYPQSSLDAKISISLEVPE
FT                   YETSAAFRNGFILQRTKSRSTYTFNDKMPLHLDLTKVTTTRRNSHQYTSHEVEVEMDPI
FT                   FKETISANDREKFNEYMCSFLNASDLIRKAAERDNMLTT"
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SQ   Sequence 963 BP; 335 A; 201 C; 180 G; 247 T; 0 other;
     atgtctgacc aacccgagac tccttccaat agtagaaatt ctcatgaaaa tgttggcgca        60
     aagaaagctg acgctaatgt tgcctcgaaa ttcagaagct tgcatatctc tgaaaccact       120
     aagccattaa catcaactag agctttatac aaaaccacaa gaaacaattc aagaggtgcg       180
     acagaattcc ataaacatgt ttgtaagtta gcatggaaat atcttgcttg tattgataaa       240
     tcgtcgatat cacacattga aatcgaaatg aaatttggtg ttataacaga caaaaggaca       300
     catcgcagga tgacaccaca caacaagccc tttattgttc aaaacagaaa tggccgctta       360
     gtgtctaacg ttcctgagca aatgttctcg agtttccagg agctactccg ctcaaaatcg       420
     gagaatccaa gtaaatgtgc cccaagagta gtaaaacagg tacaaaagta tacgaaagat       480
     tctatatata actgcaacaa tgcctcgaaa gttggcaaat taacttcatg gcgttgttcg       540
     gaagacctta gaaacaaaga acttaaactt acttacatca aaaaggtccg ggtcaaggat       600
     tttctcattc gctatcctca aagttcatta gacgctaaaa ttagcatcag ccttgaagtg       660
     cctgaatacg aaacttctgc cgctttcagg aatggcttta ttttacaaag aaccaaaagc       720
     cgttccacct acacctttaa tgataaaatg cctttacact tggatttgac aaaggttact       780
     accacgcgaa ggaactccca tcaatacacg tcccatgaag tggaggtaga aatggatcct       840
     atattcaagg agacaatttc cgctaatgat agggagaagt ttaatgagta tatgtgctcg       900
     tttttgaatg ccagcgacct tatacgtaag gctgcagaac gcgataacat gttaacgaca       960
     tga                                                                     963
//
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