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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AY692802; SV 1; linear; genomic DNA; STD; FUN; 843 BP.
XX
AC   AY692802;
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DT   11-AUG-2004 (Rel. 80, Created)
DT   01-MAY-2007 (Rel. 91, Last updated, Version 2)
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DE   Saccharomyces cerevisiae clone FLH158284.01X YGL224C gene, complete cds.
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KW   Yeast ORF Project.
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OS   Saccharomyces cerevisiae (baker's yeast)
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
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RN   [1]
RP   1-843
RX   DOI; 10.1101/gr.6037607
RX   PUBMED; 17322287.
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae";
RL   Genome Res. 17(4):536-543(2007).
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RN   [2]
RP   1-843
RA   Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E.,
RA   Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., Zuo D.,
RA   Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., Gonzaga L.,
RA   Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., LaBaer J.;
RT   "Creation of the YFLEX clone resource: cloning of Saccharomyces cerevisiae
RT   ORFs in the Gateway recombinational cloning system";
RL   Unpublished.
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RN   [3]
RP   1-843
RA   Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E.,
RA   Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., Zuo D.,
RA   Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., Gonzaga L.,
RA   Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., LaBaer J.;
RT   ;
RL   Submitted (20-JUL-2004) to the EMBL/GenBank/DDBJ databases.
RL   Biological Chemistry and Molecular Pharmacology, Harvard Institute of
RL   Proteomics, 320 Charles st., Cambridge, MA 02141, USA
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DR   ImaGenes; RZPDe861F04127D.
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CC   This clone is part of a collection of Saccharomyces cerevisiae
CC   full-length ORF clones generated by the Harvard Institute of
CC   Proteomics. Each CDS has been cloned with its native stop-codon.
CC   The CDS has been directionally cloned using the Gateway  cloning
CC   system into the donor vectors pDONR 201 or pDONR 221. Additional
CC   sequences in the clone:  'TCCAGCTGACCACC' after the attL1 site and
CC   before the 'ATG' (from Research Genetics primers used to amplify
CC   the ORFs, including a Kozak consensus sequence);
CC   'ATCCCCGGGAATTGCCATG' after the stop codon and before the attL2
CC   site (from the Research Genetics primers used to amplify the ORFs).
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FH   Key             Location/Qualifiers
FH
FT   source          1..843
FT                   /organism="Saccharomyces cerevisiae"
FT                   /lab_host="DH5alpha T1 resistant"
FT                   /mol_type="genomic DNA"
FT                   /clone="FLH158284.01X"
FT                   /db_xref="taxon:4932"
FT   mRNA            <1..>843
FT                   /product="YGL224C"
FT   CDS             1..843
FT                   /codon_start=1
FT                   /product="YGL224C"
FT                   /db_xref="GOA:P53078"
FT                   /db_xref="InterPro:IPR010237"
FT                   /db_xref="SGD:S000003192"
FT                   /db_xref="UniProtKB/Swiss-Prot:P53078"
FT                   /protein_id="AAT92821.1"
FT                   /translation="MTVEYTASDLATYQNEVNEQIAKNKAHLESLTHPGSKVTFPIDQD
FT                   ISATPQNPNLKVFFFDIDNCLYKSSTRIHDLMQQSILRFFQTHLKLSPEDAHVLNNSYY
FT                   KEYGLAIRGLVMFHKVNALEYNRLVDDSLPLQDILKPDIPLRNMLLRLRQSGKIDKLWL
FT                   FTNAYKNHAIRCLRLLGIADLFDGLTYCDYSRTDTLVCKPHVKAFEKAMKESGLARYEN
FT                   AYFIDDSGKNIETGIKLGMKTCIHLVENEVNEILGQTPEGAIVISDILELPHVVSDLF"
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SQ   Sequence 843 BP; 280 A; 154 C; 169 G; 240 T; 0 other;
     atgactgttg aatatactgc atccgatttg gcaacctatc agaatgaggt gaacgaacaa        60
     attgccaaga ataaagcaca tcttgaatca ttgactcatc caggttcgaa agtgacattt       120
     ccgatcgatc aagatatttc tgctacacct caaaacccga acttaaaagt tttctttttt       180
     gatatcgaca actgtctcta taaatcatcc acgagaattc acgatcttat gcaacaatct       240
     atactgaggt tctttcaaac acacttgaaa ctatcacctg aagatgctca cgttttgaat       300
     aactcttact acaaagagta tggacttgcc ataaggggac ttgtaatgtt ccacaaagtg       360
     aacgcactgg aatataaccg gcttgtggat gattccttac cactacaaga tatcttgaaa       420
     cctgatatac cattaaggaa tatgctgttg agattgagac aatcgggaaa gattgataaa       480
     ttgtggcttt tcaccaacgc ttataaaaac cacgcaatac gttgcctgag attgttgggt       540
     atagcggatt tatttgatgg attgacatat tgtgactact ctaggacaga tacgctggtc       600
     tgcaaaccac atgttaaagc atttgaaaaa gccatgaagg aaagcgggtt ggcaagatac       660
     gagaatgctt attttataga tgacagtggg aaaaacatcg agacaggtat caaattgggc       720
     atgaaaacat gtattcattt agttgagaat gaagtaaatg aaattttggg gcaaacacca       780
     gaaggcgcta ttgtcattag tgatatattg gagttaccac acgttgtgtc cgacctgttc       840
     taa                                                                     843
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