 |
|
EBI DbfetchID AY692644; SV 1; linear; genomic DNA; STD; FUN; 1401 BP. XX AC AY692644; XX DT 11-AUG-2004 (Rel. 80, Created) DT 01-MAY-2007 (Rel. 91, Last updated, Version 3) XX DE Saccharomyces cerevisiae clone FLH158228.01X YBL086C gene, complete cds. XX KW Yeast ORF Project. XX OS Saccharomyces cerevisiae (baker's yeast) OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. XX RN [1] RP 1-1401 RX DOI; 10.1101/gr.6037607 RX PUBMED; 17322287. RA Hu Y., Rolfs A., Bhullar B., Murthy T.V., Zhu C., Berger M.F., RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J., RA Simpson A., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.; RT "Approaching a complete repository of sequence-verified protein-encoding RT clones for Saccharomyces cerevisiae"; RL Genome Res. 17(4):536-543(2007). XX RN [2] RP 1-1401 RA Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E., RA Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., Zuo D., RA Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., Gonzaga L., RA Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., LaBaer J.; RT "Creation of the YFLEX clone resource: cloning of Saccharomyces cerevisiae RT ORFs in the Gateway recombinational cloning system"; RL Unpublished. XX RN [3] RP 1-1401 RA Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E., RA Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., Zuo D., RA Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., Gonzaga L., RA Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., LaBaer J.; RT ; RL Submitted (20-JUL-2004) to the EMBL/GenBank/DDBJ databases. RL Biological Chemistry and Molecular Pharmacology, Harvard Institute of RL Proteomics, 320 Charles st., Cambridge, MA 02141, USA XX DR ImaGenes; RZPDe861D09126D. XX CC This clone is part of a collection of Saccharomyces cerevisiae CC full-length ORF clones generated by the Harvard Institute of CC Proteomics. Each CDS has been cloned with its native stop-codon. CC The CDS has been directionally cloned using the Gateway cloning CC system into the donor vectors pDONR 201 or pDONR 221. Additional CC sequences in the clone: 'TCCAGCTGACCACC' after the attL1 site and CC before the 'ATG' (from Research Genetics primers used to amplify CC the ORFs, including a Kozak consensus sequence); CC 'ATCCCCGGGAATTGCCATG' after the stop codon and before the attL2 CC site (from the Research Genetics primers used to amplify the ORFs). XX FH Key Location/Qualifiers FH FT source 1..1401 FT /organism="Saccharomyces cerevisiae" FT /lab_host="DH5alpha T1 resistant" FT /mol_type="genomic DNA" FT /clone="FLH158228.01X" FT /db_xref="taxon:4932" FT mRNA <1..>1401 FT /product="YBL086C" FT CDS 1..1401 FT /codon_start=1 FT /product="YBL086C" FT /db_xref="InterPro:IPR019448" FT /db_xref="SGD:S000000182" FT /db_xref="UniProtKB/Swiss-Prot:P38177" FT /protein_id="AAT92663.1" FT /translation="MPFNHNSKAKRPKFLLDLQIKELVNIPQSSGYCYTKWRLKDGTGT FT SGHKVALDGEHQTTSTQSRGTTKHVHVQHHRAQWNYSLDKPILVKLHLDKNGRFLKKIL FT VLDVFFEFADANSSLTSSSSPNGKVKKTTYANATALTATGNNSYSQKITGKLLLGTVDI FT DITEYVKEDETPTTNRFLLKHSKVNSIINVSLQLKLVRGSYEDFNISKSFTNGQLANYR FT PGINTILDNTSELSSPTSTTNQMSPKNTFSNFNGIGTTVAKPGTNATGNSTSIKSPTST FT NHKSSEMTTKPGLSTTISSSMSPLIESLYQKTFKLPRDPRPGEFTPRECVEDILQGGNG FT WAKNEKGINLIDLQALRLNEMEEEYYNPNYGNNLGNKASSWPPNPSDDGYSTMGKREYL FT EKKQNWSHMSRAQRAKLRTHNDEDNENTANDKGSDKDNNSVEDNNPTDFLTDRIRENKN FT WSIITPSG" FT variation 952 FT /replace="t" FT /note="compared to SGD sequence" XX SQ Sequence 1401 BP; 511 A; 290 C; 288 G; 312 T; 0 other; atgccattta atcataatag caaggcaaaa agaccaaagt ttctactgga cctgcaaatc 60 aaagaattgg taaatattcc tcaatcttct ggctattgct acacgaaatg gcgtttgaag 120 gatggaacag gtacgtcggg acacaaagtg gctctggacg gtgagcatca aaccacttcg 180 acccagagta gaggtacaac aaaacacgtg catgtgcaac atcatagagc acaatggaat 240 tattcattag acaaaccaat cctggtgaag ctccacttgg ataaaaacgg cagattcttg 300 aagaaaattt tggtgttgga tgtttttttt gagtttgctg atgcgaattc ctcgttgact 360 tctagttcta gtcccaatgg caaagtgaag aaaacgacct atgctaacgc cactgcacta 420 acggccacag gaaacaattc atattctcaa aagataacgg gtaaacttct attgggaaca 480 gtagacatag atataacaga atacgtcaaa gaagacgaaa ctcccacaac aaacagattt 540 ctcctaaagc actcaaaggt caattctata atcaacgtct cattgcagtt gaagctagtt 600 agaggctctt acgaagattt caacatttcc aaatcgttta ctaatggcca gctcgctaat 660 tacaggccag gaataaatac tatactggat aacacgtctg aactatctag ccccacatca 720 acaacaaacc agatgtcacc caagaataca ttctccaatt ttaacggtat tggaacaact 780 gtagcgaaac ccggtacaaa tgcgacagga aacagcacaa gtattaaatc accgacttct 840 accaaccaca agagtagcga aatgacaaca aagccagggc tgtcaacaac aatatcttcc 900 tctatgagcc cactgattga gagcttgtac cagaaaacgt ttaaattacc gcgggatcct 960 cgaccaggtg aatttacgcc aagagaatgc gtggaagata ttttacaagg cggaaacggt 1020 tgggctaaga acgagaaggg cataaattta atcgatttac aggcattgag attgaacgaa 1080 atggaagaag agtactacaa tccaaattat ggcaataacc ttgggaacaa ggcaagtagt 1140 tggcctccaa atccaagcga tgacggctac agcacaatgg gcaaaagaga gtatttggag 1200 aaaaagcaga attggagtca tatgtcacga gcacaacgtg caaaactgag gactcataat 1260 gacgaggata acgaaaatac ggctaatgat aaagggagcg acaaggataa taactctgtc 1320 gaagacaata accccacgga ctttttaaca gatagaataa gagaaaacaa aaactggtct 1380 ataataaccc catcgggcta g 1401 // |
|