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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AY558104; SV 1; linear; genomic DNA; STD; FUN; 808 BP.
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AC   AY558104;
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DT   16-MAR-2004 (Rel. 79, Created)
DT   01-MAY-2007 (Rel. 91, Last updated, Version 2)
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DE   Saccharomyces cerevisiae clone FLH113403.01X YHL026C gene, complete cds.
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KW   Yeast ORF Project.
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OS   Saccharomyces cerevisiae (baker's yeast)
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
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RN   [1]
RP   1-808
RX   DOI; 10.1101/gr.6037607
RX   PUBMED; 17322287.
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae";
RL   Genome Res. 17(4):536-543(2007).
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RN   [2]
RP   1-808
RA   Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E.,
RA   Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., Zuo D.,
RA   Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., Gonzaga L.,
RA   Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., LaBaer J.;
RT   "Creation of the YFLEX clone resource: cloning of Saccharomyces cerevisiae
RT   ORFs in the Gateway recombinational cloning system";
RL   Unpublished.
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RN   [3]
RP   1-808
RA   Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E.,
RA   Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., Zuo D.,
RA   Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., Gonzaga L.,
RA   Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., LaBaer J.;
RT   ;
RL   Submitted (17-FEB-2004) to the EMBL/GenBank/DDBJ databases.
RL   Biological Chemistry and Molecular Pharmacology, Harvard Institute of
RL   Proteomics, 320 Charles St., Cambridge, MA 02141, USA
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DR   ImaGenes; RZPDo838G04112D.
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CC   This clone is part of a collection of Saccharomyces cerevisiae
CC   full-length ORF clones generated by the Harvard Institute of
CC   Proteomics. Each CDS has been cloned with its native stop-codon.
CC   The CDS has been directionally cloned using the Gateway cloning
CC   system into the donor vectors pDONR 201 or pDONR 221. Additional
CC   sequences in the clone:  'TCCAGCTGACCACC' after the attL1 site and
CC   before the 'ATG' (from Research Genetics primers used to amplify
CC   the ORFs, including a Kozak consensus sequence);
CC   'ATCCCCGGGAATTGCCATG' after the stop codon and before the attL2
CC   site (from the Research Genetics primers used to amplify the ORFs).
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FH   Key             Location/Qualifiers
FH
FT   source          1..808
FT                   /organism="Saccharomyces cerevisiae"
FT                   /lab_host="Escherichia coli DH5alpha T1 resistant"
FT                   /mol_type="genomic DNA"
FT                   /clone="FLH113403.01X"
FT                   /db_xref="taxon:4932"
FT   mRNA            <1..>807
FT                   /product="YHL026C"
FT   CDS             1..807
FT                   /codon_start=1
FT                   /product="YHL026C"
FT                   /db_xref="GOA:P38740"
FT                   /db_xref="InterPro:IPR018852"
FT                   /db_xref="SGD:S000001018"
FT                   /db_xref="UniProtKB/Swiss-Prot:P38740"
FT                   /protein_id="AAS56430.1"
FT                   /translation="MYHSRVDLITLWAFPHTISGDCALSLFIQVGLTWASEEILVGFDD
FT                   YKRPVFRLNKWITKPSPLKTESNEEIPPPKKRFIVDYFESKDNVVAKQNTLYHKHNWLF
FT                   GYLEVNRGIIPKGKEATLKGFLTSQFIHDSTQSKFMNFIEWFVQKFIRSMILAIAMFIV
FT                   IWPVTMGILAGIGHKVGSHDYYFNDYPLPQVMKLIYAVVIAFVCTPVAIIVIVLRNQFH
FT                   EELYYEGLANGTLQQDQEVCSTGNRSSGSTDQDISTTKQQSQEAVA"
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SQ   Sequence 808 BP; 233 A; 179 C; 167 G; 229 T; 0 other;
     atgtaccatt cccgtgtaga tcttatcacc ttatgggcat ttcctcacac catttcaggc        60
     gattgtgcac taagtttatt catccaggtg ggtcttacat gggctagcga ggaaatttta       120
     gttggattcg atgactacaa gaggcctgtt ttcagattga ataaatggat tacgaaaccg       180
     tcaccactga aaaccgaatc taacgaagaa ataccacccc ctaaaaagcg tttcattgtg       240
     gattacttcg aatccaaaga caacgtcgtt gcgaagcaga acactctgta tcataagcat       300
     aattggctct ttggctattt agaggtcaat agaggaatca ttcccaaagg taaagaagct       360
     acgttgaaag gttttctcac atctcagttc attcatgatt ctactcagtc caaattcatg       420
     aactttatag aatggtttgt gcagaaattc atcagatcta tgatcctagc cattgccatg       480
     ttcattgtca tttggccagt cactatgggt atccttgctg gtataggcca taaggtcggg       540
     tcccacgact actacttcaa cgattatcca ctaccgcagg tcatgaagtt gatttatgcc       600
     gttgtaattg cctttgtatg cactccagtg gccatcatcg tcattgtgtt gagaaaccag       660
     tttcacgagg aactttacta tgaaggctta gcaaacggta cgctacagca ggatcaagaa       720
     gtttgctcca cgggtaatcg cagctcggga tcaaccgatc aagatatcag tactaccaaa       780
     caacagtctc aagaagcggt agcgtgac                                          808
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