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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AY557940; SV 1; linear; genomic DNA; STD; FUN; 702 BP.
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AC   AY557940;
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DT   16-MAR-2004 (Rel. 79, Created)
DT   01-MAY-2007 (Rel. 91, Last updated, Version 2)
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DE   Saccharomyces cerevisiae clone FLH004569.01X YLL060C gene, complete cds.
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KW   Yeast ORF Project.
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OS   Saccharomyces cerevisiae (baker's yeast)
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
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RN   [1]
RP   1-702
RX   DOI; 10.1101/gr.6037607
RX   PUBMED; 17322287.
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae";
RL   Genome Res. 17(4):536-543(2007).
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RN   [2]
RP   1-702
RA   Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E.,
RA   Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., Zuo D.,
RA   Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., Gonzaga L.,
RA   Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., LaBaer J.;
RT   "Creation of the YFLEX clone resource: cloning of Saccharomyces cerevisiae
RT   ORFs in the Gateway recombinational cloning system";
RL   Unpublished.
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RN   [3]
RP   1-702
RA   Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E.,
RA   Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., Zuo D.,
RA   Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., Gonzaga L.,
RA   Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., LaBaer J.;
RT   ;
RL   Submitted (17-FEB-2004) to the EMBL/GenBank/DDBJ databases.
RL   Biological Chemistry and Molecular Pharmacology, Harvard Institute of
RL   Proteomics, 320 Charles St., Cambridge, MA 02141, USA
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DR   ImaGenes; RZPDo838H04101D.
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CC   This clone is part of a collection of Saccharomyces cerevisiae
CC   full-length ORF clones generated by the Harvard Institute of
CC   Proteomics. Each CDS has been cloned with its native stop-codon.
CC   The CDS has been directionally cloned using the Gateway cloning
CC   system into the donor vectors pDONR 201 or pDONR 221. Additional
CC   sequences in the clone:  'TCCAGCTGACCACC' after the attL1 site and
CC   before the 'ATG' (from Research Genetics primers used to amplify
CC   the ORFs, including a Kozak consensus sequence);
CC   'ATCCCCGGGAATTGCCATG' after the stop codon and before the attL2
CC   site (from the Research Genetics primers used to amplify the ORFs).
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FH   Key             Location/Qualifiers
FH
FT   source          1..702
FT                   /organism="Saccharomyces cerevisiae"
FT                   /lab_host="Escherichia coli DH5alpha T1 resistant"
FT                   /mol_type="genomic DNA"
FT                   /clone="FLH004569.01X"
FT                   /db_xref="taxon:4932"
FT   mRNA            <1..>702
FT                   /product="YLL060C"
FT   CDS             1..702
FT                   /codon_start=1
FT                   /product="YLL060C"
FT                   /note="GTT2"
FT                   /db_xref="GOA:Q12390"
FT                   /db_xref="HSSP:1E6B"
FT                   /db_xref="InterPro:IPR012335"
FT                   /db_xref="SGD:S000003983"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q12390"
FT                   /protein_id="AAS56266.1"
FT                   /translation="MNGRGFLIYNGGEKMKQKMIIYDTPAGPYPARVRIALAEKNMLSS
FT                   VQFVRINLWKGEHKKPEFLAKNYSGTVPVLELDDGTLIAECTAITEYIDALDGTPTLTG
FT                   KTPLEKGVIHMMNKRAELELLDPVSVYFHHATPGLGPEVELYQNKEWGLRQRDKALHGM
FT                   HYFDTVLRERPYVAGDSFSMADITVIAGLIFAAIVKLQVPEECEALRAWYKRMQQRPSV
FT                   KKLLEIRSKSS"
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SQ   Sequence 702 BP; 212 A; 157 C; 167 G; 166 T; 0 other;
     atgaatggca gaggtttcct gatttacaat ggaggtgaaa agatgaaaca aaaaatgata        60
     atatatgaca cacccgcagg gccttatccg gcccgagtcc gcattgcctt ggctgagaag       120
     aacatgctat caagtgtgca atttgtgagg atcaacctct ggaagggaga gcacaagaag       180
     cctgaatttc ttgccaagaa ctattcaggc acagtgccag tgcttgaact tgatgacggg       240
     actttaatcg ctgaatgcac agccattact gaatacattg atgcacttga tggtacaccc       300
     actcttaccg gcaaaacacc gctggaaaaa ggcgtaatcc acatgatgaa caaacgcgca       360
     gagttggaac tgctcgaccc tgttagtgtt tattttcacc atgctacacc tggattgggg       420
     cctgaagtcg agctttacca aaacaaagag tggggacttc gccagcgcga caaagcccta       480
     catggaatgc attattttga taccgttctc agagaacgcc catatgttgc tggtgattca       540
     ttctcaatgg ctgacatcac agtaatagct ggtctaatat ttgctgcaat tgtaaaacta       600
     caagtgccgg aagagtgcga ggcgcttcga gcttggtata agagaatgca acagcgcccc       660
     agcgtgaaga aactgctaga aatccgttca aaatcctcgt aa                          702
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