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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AY557786; SV 1; linear; genomic DNA; STD; FUN; 1017 BP.
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AC   AY557786;
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DT   16-MAR-2004 (Rel. 79, Created)
DT   01-MAY-2007 (Rel. 91, Last updated, Version 2)
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DE   Saccharomyces cerevisiae clone FLH111561.01X YER156C gene, complete cds.
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KW   Yeast ORF Project.
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OS   Saccharomyces cerevisiae (baker's yeast)
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
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RN   [1]
RP   1-1017
RX   DOI; 10.1101/gr.6037607
RX   PUBMED; 17322287.
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae";
RL   Genome Res. 17(4):536-543(2007).
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RN   [2]
RP   1-1017
RA   Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E.,
RA   Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., Zuo D.,
RA   Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., Gonzaga L.,
RA   Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., LaBaer J.;
RT   "Creation of the YFLEX clone resource: cloning of Saccharomyces cerevisiae
RT   ORFs in the Gateway recombinational cloning system";
RL   Unpublished.
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RN   [3]
RP   1-1017
RA   Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E.,
RA   Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., Zuo D.,
RA   Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., Gonzaga L.,
RA   Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., LaBaer J.;
RT   ;
RL   Submitted (17-FEB-2004) to the EMBL/GenBank/DDBJ databases.
RL   Biological Chemistry and Molecular Pharmacology, Harvard Institute of
RL   Proteomics, 320 Charles St., Cambridge, MA 02141, USA
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DR   ImaGenes; RZPDo838B11109D.
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CC   This clone is part of a collection of Saccharomyces cerevisiae
CC   full-length ORF clones generated by the Harvard Institute of
CC   Proteomics. Each CDS has been cloned with its native stop-codon.
CC   The CDS has been directionally cloned using the Gateway cloning
CC   system into the donor vectors pDONR 201 or pDONR 221. Additional
CC   sequences in the clone:  'TCCAGCTGACCACC' after the attL1 site and
CC   before the 'ATG' (from Research Genetics primers used to amplify
CC   the ORFs, including a Kozak consensus sequence);
CC   'ATCCCCGGGAATTGCCATG' after the stop codon and before the attL2
CC   site (from the Research Genetics primers used to amplify the ORFs).
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FH   Key             Location/Qualifiers
FH
FT   source          1..1017
FT                   /organism="Saccharomyces cerevisiae"
FT                   /lab_host="Escherichia coli DH5alpha T1 resistant"
FT                   /mol_type="genomic DNA"
FT                   /clone="FLH111561.01X"
FT                   /db_xref="taxon:4932"
FT   mRNA            <1..>1017
FT                   /product="YER156C"
FT   CDS             1..1017
FT                   /codon_start=1
FT                   /product="YER156C"
FT                   /db_xref="GOA:P40093"
FT                   /db_xref="InterPro:IPR003226"
FT                   /db_xref="SGD:S000000958"
FT                   /db_xref="UniProtKB/Swiss-Prot:P40093"
FT                   /protein_id="AAS56112.1"
FT                   /translation="MNSVKRVKLNSKMSKQICTHSGSFHADESLAVYMLRLLPEFKDAK
FT                   LVRSRNPKDWEASDILVDVGAQYDGVKFFDHHQRGFFETFNEKYKTKLSSAGLIFKHYG
FT                   RDIIKTILNNKVSSSDLDLLYDKVYKQFVEALDANDNGISKYTIPKDSNLEPNFRDNAI
FT                   SIPGIISGMNPNWNEDTSDESFDRCFARASEFIGGVFVTLVRGYGQSWLPAKALVAQAI
FT                   DERMDVDKSGKIIVLPQFCPWKEHLYELEREKNIEKQIEFVLFTDSSGAWRVSTVPINS
FT                   TSFQFRRGLPEPLRGLRDEELSTKSGVPGCIFIHAAGFIGGAKSKEAVYELAKMSLA"
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SQ   Sequence 1017 BP; 299 A; 207 C; 249 G; 262 T; 0 other;
     atgaatagcg taaaaagagt aaagctaaat tcaaaaatgt ccaaacaaat atgcacgcat        60
     tccggatctt ttcacgcgga cgagtcgttg gcggtataca tgttaaggct actacccgaa       120
     tttaaagatg ctaagttggt tagatcaaga aatcccaagg actgggaggc tagtgacatt       180
     ctagtggatg taggagctca gtatgacggc gtgaaatttt ttgaccacca ccagcgtgga       240
     tttttcgaaa ctttcaacga gaaatacaag actaagctct caagtgctgg tctgatcttc       300
     aaacactacg gtcgtgacat tatcaaaaca attttgaaca acaaggttag cagctctgat       360
     ttagatctgc tctatgacaa ggtatacaaa caatttgtcg aggcactgga tgcaaacgac       420
     aacggtatta gcaagtacac gattcctaag gattccaact tggaaccgaa tttcagggat       480
     aacgccatca gcattcccgg gattatttca ggtatgaatc ctaactggaa cgaagacact       540
     tccgatgagt cttttgacag atgctttgct cgcgcaagtg agtttatcgg tggggtcttt       600
     gtcacattgg taagaggcta cggtcaatcg tggttgccag ccaaggccct tgtggcgcaa       660
     gccattgatg aaagaatgga tgttgataag agtggcaaaa tcattgtctt gccccaattc       720
     tgtccatgga aggagcatct atacgagctg gaaagagaga agaacattga aaagcaaatc       780
     gagtttgttc tcttcacaga ctcttctggt gcctggaggg tatctactgt accaatcaac       840
     tccacctcct tccagtttag gagaggtttg ccggagccat taagaggcct cagagacgaa       900
     gagctgagca ccaagagcgg tgtgccagga tgtatcttca ttcatgcagc aggtttcatt       960
     ggaggtgcca agtccaaaga ggctgtatat gagctggcta agatgtcttt ggcctag         1017
//
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